... functions of DMS and compounds on SPHK1 and SPHK2 activity A, Inhibition of SPHK1 activity by various concentrations of compounds and DMS is shown B, Inhibition of SPHK2 activity by various concentrations ... understanding of the roleof SPHK in the process of stem cells differentiation will be studied as well In this chapter, only the compounds synthesis and evaluation are discussed Compounds function on ... Development and Evaluation of Human SPHK Inhibitors figures Stimulation was stopped by adding v/v of ice-cold 1x PBS The cell samples were collected (by centrifugation) and lysed (Section 2.1.2.1.3 Cell...
... Osteogenic Differentiation In human BM- and AD- MSCs osteogenic differentiation study, hFOB cells were used as control cells, as this cell line is capable of osteoblastic differentiationand bone formation ... (hFOB) Cell Line The hFOB cell line is a clonal, conditionally immortalized human fetal cell line that is capable of osteoblastic differentiationand bone formation It was used as a positive control ... Osteocalcin, Osteopontin, and Osteonectin Expression of DMS/CP6 treated Stem Cells in the Osteogenic Differentiation Besides detecting the degree of the osteogenic differentiationof human BM- and ADMSCs...
... 10 cell growth 120% culture conditions 131 Chapter Roleof S1P in human BM- and AD- MSCs proliferation Figure 4.6 Function of S1P and serum in human BM- and AD- MSCs proliferation Human BM -and ... culture conditions Figure 4.7 S1P and PDGF-AB function in human BM- and AD- MSCs proliferation Human BM- and AD- MSCs were cultured in different culture conditions and cells proliferation were ... took them too long for the cells grew confluent, only the cells from DMEM+10%FBS and 136 Chapter Roleof S1P in human BM- and AD- MSCs proliferation DMEM+10%FBS+1μM S1P culture conditions were analyzed...
... expanded to study other stem cells and other differentiations pathways, to further validate and confirm the potential usage of SPHK inhibitors in stem cell differentiation, and S1P roles on stem ... more controlled and facilitate differentiationof stem cells, for future 154 Chapter Conclusion and Future Work therapeutic applications to balance the abnormal portions of osteoblasts and adipocytes ... MSCs differentiation in the mixture of both osteogenic differentiation media and adipogenic differentiation media, which may simulate a more in vivo environment By optimizing the concentrations of...
... "Functional neuronal differentiationof bone marrowderived mesenchymal stem cells." Stem Cells 24(12): 2868-76 Tseng, S C and D Q Li (1996) "Comparison of protein kinase C subtype expression between ... "Isolation and expansion of adult cardiac stem cells from human and murine heart." Circ Res 95(9): 911-21 Mets, T and G Verdonk (1981) "In vitro aging of human bone marrow derived stromal cells." ... muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: effect of dexamethasone." J Cell Biol 106(6): 2139-51 Hait, Nitai C., Andrea Bellamy, et al...
... stem celldifferentiation A number of studies have reported a role for S1P in cellproliferationand differentiation, but not much has been reported for a role for SPHK in cell differentiation, ... applications and significance of using SPHK and S1P in stem cells research, derived from the current understanding on human stem cells and the strategies used for stem cell expansion and differentiation, ... physiological and pathophysiological processes Activation of SPHK and accumulation of S1P are believed to be involved in cell proliferation, including in embryonic stem cellproliferation (Pébay...
... FGF-2 and BMP-2 are required at different stages of bone repair Table Mineralization of cells with BMP-2 Conclusions These data demonstrate the de -differentiation (reduction of mineralization genes) ... mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related ... mineralization stage is associated with the sequential expression of genes that support biosynthesis, organization and mineralization of the bone extracellular matrix Mineralization requires expression of...
... FGF-2 and BMP-2 are required at different stages of bone repair Table Mineralization of cells with BMP-2 Conclusions These data demonstrate the de -differentiation (reduction of mineralization genes) ... mineralization Figure FGF-2 and BMP-2, the yin and yang of mineralization: Contrast of effect of 24 hours of treatment with FGF-2 or BMP2 on fold increase in abundance of mineralization-related ... mineralization stage is associated with the sequential expression of genes that support biosynthesis, organization and mineralization of the bone extracellular matrix Mineralization requires expression of...
... cytoskeleton 2.6 Effects of stiffness of substrate on focal adhesion 2.7 Focal adhesion points in relation to cellproliferation 2.8 Formation of focal adhesion points 2.8.1 Marker of focal adhesions ... Initial contact ofcell with solid substrate (B) Formation of bonds between cell surface receptors andcell adhesion ligands (C) Cytoskeletal reorganization with progressive spreading of the cellon ... background information on the wide biomedical applications of PCL and the cellular responses such as growth, proliferationand also focal adhesion contact points when cells are seeded onto a substrate...
... Inhibition of PAR-2 expression augments H pyloriinduced cell death and DNA fragmentation in gastric epithelial cells To investigate the relations of PAR-2 expression, cell death, and DNA fragmentation, ... epithelial cells To determine the roleof PAR-2 on the activation of MAPK, the activation of three major MAPK involved in cellproliferationand apoptosis were assessed by Western blotting of phospho-specific ... the cells without transfection and cultured in the presence of H pylori Figure Inhibition of PAR-2 expression augments H pyloriinduced cell death and DNA fragmentation in AGS cells (A) AGS cells...
... toxicity of each borax concentration on immune cellproliferation (lymphocyte proliferation) by MTT assay and study about the genotoxicity effect were done by ANOVA test Figure The correlation between ... duplicate fashion Mean absorbance was calculate for the control wells and for each borax concentration in the test wells The degree of immune cellproliferation (lymphocyte proliferation) sensitivity ... Borax effects on immune cellproliferation (lymphocyte proliferation) the number of living lymphocytes The results indicated that at the borax concentrations of 0.15, 0.2, 0.3 and 0.6 mg/ml,...
... isolation and characterization of MSCs; MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T -cell proliferation; design of ... study; and manuscript preparation TM and HK contributed to MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; and analysis of T -cell proliferation ... expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T -cell inhibition The involvement of iNOS and COX-2 in inhibition of T -cell proliferation was demonstrated...
... THE ROLEOF MICRORNAS IN EMBRYONIC STEM CELL DEVELOPMENT ANDDIFFERENTIATION TAY MEI SIAN YVONNE B.Sc (Hons), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY NUS Graduate ... demonstrate a high degree of correlation 3.7 MiR-134 enhances RA- and N2B27-mediated mESC differentiation 48 3.8 Discussion 51 MICRORNA TARGET PREDICTION 53 4.1 Introduction 53 4.2 Validation of ... 8.2.4 Construction of pLuc-MRE plasmids 122 8.2.5 Construction of gene-specific RNAi plasmids 123 8.2.6 Construction of Nanog-CDS plasmids 124 8.3 RNA and protein work 126 8.3.1 RNA extraction &...
... effect of high concentrations of glucose on the growth, survival, proliferationand cell- fate specification of NSCs isolated from the telencephalon of embryonic mice, andon the expression of some ... 2.3 Cell fate specification of NSCs exposed to high glucose ···························80 2.4 Cellproliferationand neuronal differentiationof NSCs in the telencephalon of E11.5 embryos of diabetic ... EFFECTS OF HIGH GLUCOSE CONCENTRATIONS ON THE EXPRESSION OF GENES INVOLVED IN PROLIFERATIONAND CELL- FATE SPECIFICATION OF MOUSE EMBRYONIC NEURAL STEM CELLS FU JIANG (MD, MMed)...
... function is generally required for cellproliferationandcell growth or whether its essential role in proliferation is restricted to specific cell types Control ofCellProliferationand Growth ... induction of cyclin D2 by Myc contributes to cell cycle progression and sequestration of p27 EMBO J 18:5321–5333 Control ofCellProliferationand Growth by Myc Proteins 339 Chen D, Kon N, Li ... deletion of the mnt gene, which encodes a member of the Mad family of antagonists of Myc, restores proliferation to c-myc deleted cells, suggesting that the balance of expression of members of the...
... EDUCATION AND PRODUCTION FUNCTIONS ances of A with log Y, but we have information on the relationship of A to S If we ignore A and estimate a truncated relationship of log Y on S for each region ... education 15 See Griliches [261 and Lancaster (411 for a recent survey and exposition of such an approach 90 EDUCATION AND PRODUCTION FUNCTIONS sional version of such an approach.16 Each person ... level of aggregation • • I - — I 82 EDUCATION AND PRODUCTION FUNCTIONS TABLE Education and Skill Variables in Aggregate Production Function Studies Industry, Unit of Observation Period and Sample...
... proliferation (Eur J Biochem 270) 109 cellular events that are susceptible to inhibition by MIBG and should permit further examination of the roleof mono-ADP-ribosylation in cell cycle control andcell ... phenotypic modulation, and consequently proliferationof SMC Furthermore, Thyberg et al [20] concluded that poly(ADP-ribosyl)ation and mono-ADP-ribosylation were essential for the SMC response to mitogenic ... negative regulator of this Ó FEBS 2003 ADP-ribosylation and SMC proliferation (Eur J Biochem 270) 107 Fig Effect of MIBG on prostaglandin E2-stimulated gene expression and intracellular signalling...
... proliferationof NPCs under hypoxia, and the effect of overexpression or knockdown of HIF-1a on NPC proliferation Results Hypoxia promotes the proliferationof NPCs Neurosphere formation It has ... O2 concentration, exposure time, and type of cells In general, O2 concentrations over 1%, rather than arresting the growth of most kinds of cells, promote the proliferationof some types of cells ... the proliferationof NPCs Standard conditions for culture of mammalian cells employ about 20% O2 in vitro Exposure of cells to O2 deprivation in vitro has been shown to reduce proliferation and...
... agent concentration on number of cells per unit volume Average Cell Size (mm) 1.5 0.5 10 12 Blowing Agent Concentration (phr) Figure 3: Effect of blowing agent concentration on average cell size ... correlation between the number of cells per unit volume and the average cell size An increase in the blowing agent concentration resulted in smaller, finer, and more uniform cells The decomposition of ... Relationship between force and displacement at different blowing agent concentrations Table 3: Effect of blowing agent concentration on the absorbed energy and toughness Blowing Agent Concentration...
... on current knowledge of the regulation of the HIF pathway, and then consider the potential roleofhypoxia in the pathogenesis of RA Why is hypoxia a feature of rheumatoid arthritis? Tissue hypoxia ... recent generation of mice with specific global or conditional inactivation of each of the three PHD enzymes is very promising and will promote better understanding of the functions of the enzymes ... heterodimerization and transcriptional activity of HIF [27] The crystal structure of FIH reveals it to be a homodimeric protein [29], and disruption of dimerization of FIH, by use of site-directed...