... Ten proteins were detected as IgE-binding proteins and 8 proteins as IgG4-binding proteins, with high IgE de-tection rate and specificity. Among the IgE-binding proteins, the proteins with ... changes in fat intake since the amount of dietary fat (30% of total energy) was main-tained constant during the intervention. Similarly, in a 4-week randomized dietary intervention trial of male ... food intake and gastric emptying [69]. These effects were attributed in part to an increase in circulating levels of cholecystokinin. Similarly, in rats fed a hy-percholesterolemic diet, ingestion...
... echo-planar MR imaging andADC mapping in the differential diag-nosis of ovarian cystic masses: useful-ness of detecting keratinoid substances in mature cystic teratomas. J MagnReson Imaging 22:271–27824. ... the future of MRI in patients withgynecological diseases.Examination of the female pelvic region using DWIDWI is obtained by measuring signal loss after a series of two motion-providing gradient ... useful in detecting pelvic lymph node metastasis in patients withcervical and uterine cancers. However, the method com-bining size and relative ADC values was proposed by usinga combination of...
... targeting of CYP2E1The roleof PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches. Thefirst approach involved measuring the level of mito-chondrial targeting of wild-type ... importance of PKA in protein trafficking [47,48]. In this study, we usedPKA knockout mutants to determine the roleof PKA in mitochondrial targeting of CYP2E1. In mutantslacking PKA, CYP2E1 was ... were resuspended in SEMbuffer containing indicated concentrations of digitonin(0.05% to 0.4%, w ⁄ v) for 30 min on ice with occasionalmixing. Digitonin-soluble and digitonin-insoluble fractionswere...
... double-chaininsulin is synthesized in vivo as a single-chain precursor(preproinsulin) and folded as proinsulin, in which aconnecting peptide of 35 residues links the C terminus of the B chain and ... Sciences, Chinese Academy of Sciences, Shanghai, ChinaWe have investigated the in vitro refolding process of humanproinsulin (HPI) and an artificial mini-C derivative of HPI(porcine insulin precursor, ... character-ization of the unfolding of insulin and proinsulin using thereductive unfolding method has not been thoroughlyinvestigated. We have characterized the unfolding process of an artificial porcine insulin...
... fosfomycin binding due to charge–chargerepulsions. Most interestingly, the uncharged valine sidechain completely abolishes fosfomycin binding in theabsence of UDPNAG. Clearly, UDPNAG binding createsadditional ... notinteract with R124 in the ternary complex [20]. Hence, themechanism of binding of fosfomycin by reinforcement of aninitial binding site (mainly K22 and R397) through recruit-ment of secondary ... Lysine 22 and arginine 120 in MurA (Eur. J. Biochem. 271) 2685 the positively charged side chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin...
... that the N-terminal domain is involved in protein–protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspeciesinteractions are incompatible.From ... thebovine complex a large subdomain is made up of 20integral membrane proteins contributing > 60 transmem-brane segments. Some of these must be intimately involved in proton pumping. Another ... one might expect a protein to be insertedinto the inner membrane, but it is missing a major portion of the domain localized in the intermembrane space. Acomparison of all the known mammalian...
... family of thiolate-ligatedconstitutive or inducible hemoprotein isoforms [281],exhibiting insign ificant sequence identity with P450s in the heme-binding region [282], but bearing a C-terminalflavoprotein ... B. (1994) The pteridine b inding site of brain nitric oxidesynthase. Tetrahydrobiopterin binding k inetics, specificity, andallosteric interaction w ith the substrate d omain. J. Biol. Chem.269, ... cis-dihydroxylation of a romaticrings, in itiating aerobic degradation of aromatic compounds in soil bacteria, and are targets for bioengineering in bioremediation [204]. They a re a consortium of two o...
... periods of times (0–60 min)at 37 °C. The concentration of the biotinylated Ig remainingon the cell surface was then quantified by measuring thebinding of [125I]Streptavidin. In the absence of ... Theywere then incubated with 0.25 mL of incubation mediumcontaining 2 lgặmL)1 of biotinylated R5T-34 monoclonalIg [30] at 4 C for 30 min. In some cases, monensin 40 lMwas added to the incubation ... domain and devoid of any tag was notfunctional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag wasfound constitutively activated [29]. In agreement...
... UniversityRegional Learning Alliance – workforce development and training/collaborations with business and industry; meeting the training and education needs of businesses within 60 mile radius in Northern ... $28,000 in average annual salary)Preserving ValueContinuing education is important to preserving the market value of specialized education – particularly in professional fieldsContinuing education ... UniversitySmall Business Initiative in the Frehn Center for Management; offering information and assistance to individuals and groups planning to start a new business Indirect Contributions...
... thisprotease at pH9 [10]. Further interest in the aromaticstacking is in the roleof the electrostatic properties in enzymatic catalysis of API, and in distinguishing thefunctionally catalytic ... CH/, p interaction in thepacking of the adenine ring in protein structures. Advan. ProteinChem. 39, 125–189.20. Brandl, M., Weiss, M.S., Jabs, A.S., á hnel,J.&Hilgenfeld,R.(2001) CH p-interaction ... a new way of thinking aboutthe functional roleof the former ionizable aromatic aminoacid. The pH optimum mechanism in API results from twothings: (a) positively charged His210 interacts with...
... respectively. By introducing an Ala residue in the place of Gly it is expected that the conformational flexibility of themain chain can be constrained with a minimum perturba-tion of the local structure, ... our incomplete under-standing of the relation between enzyme properties andconformational flexibility limits the exploitation of the fullpotential of protein engineering in the redesign of psychro-philic ... the basis of side-chain packing criteriafrom a modelled structure of the enzyme resulted in restor-ation of the energy of activation to the levels of the nativeenzyme and in an increased stability...
... FactorProcessing information (perceiving, retrieving,manipulating, transmitting)Compiling information, importance of 0.90 Seeing (DOT) 0.66 Work with complex thingsCombining information, importance of ... importance of 0.36Practical problem solvingAdvising, importance of 0.86 Supervising nonemployees, importance of 0.64 Catering to peoplePlanning and scheduling, amount of 0.83 Catering and serving, ... Catering to peopleMath, level of (DOT) 0.79Math, level of 0.70Quantitative information, extent of use 0.68Coding and decoding, importance of 0.68Oral information, extent of use 0.68Talking...
... in DptF is GLDSV,indicating that this putative ACP domain is one of thefew ACPs in which valine replaces isoleucine (I) orleucine (L) in the conserved sequence. To determinewhether DptF is ... remains to be clarified whether all of the fatty acids activated by DptE can be incorpo-rated into the final product or whether there is aninterfering specificity of the CIIIdomain of the initia-tion ... buffer A in 15 min, followed by a lineargradient down to 5% buffer A in 2 min. 5% buffer A washeld for an additional 2 min and followed by a lineargradient up to 95% buffer A in 6 min.Determination...
... clearwhether or not H39 is dominantly tuning the VH⁄ VLinteraction of other Fvs, including HyHEL-10. In addition, the effect of the H39 mutation on antigenbinding has not yet been analyzed. ... improved the antigen-binding affinity of Fv,similarly to the level of scFv (Table 2).Some single VHdomains of anti-protein IgG, inclu-ding HyHEL-10 and D1.3, were known to retain thespecificity ... determining the interdomain inter-action strength.ResultsConstruction of an FR2 combinatorial libraryTo investigate the possible relationship between anti-gen-binding affinity and VH⁄ VLinteraction...
... Cardie, Vincent Ng, David Pierce,Chris Buckley. Ex-amining the roleof statistical and linguistic knowledgesources in a general-knowledge que stion answering sys-tem. In Proceedings of the 6th ... By relying on large, open-domain lin-guistic resources such as WordNet we enabled amore precise approach of searching and mininganswers from large collections of texts. Evalua-tions indicate ... parse.By implementing our own version of the pub-licly available Collins parser (Collins, 1996), wealso learned a dependency model that enables themapping of parse trees into sets of binary rela-tions...