role of a security manager in enhancing security in an organization
Tài liệu Báo cáo khoa học: Local stability identification and the role of a key aromatic amino acid residue in staphylococcal nuclease refolding pdf
Ngày tải lên :
20/02/2014, 01:20
... investigated. Two point-mutated proteins, with a single
base substitution of alanine for tryptophan (W14 0A)
and alanine for lysine (K13 3A) , and two truncated
fragment proteins, with fragments ... that tryptophan at position
140 plays an important role in maintaining protein ter-
tiary integrity. Figure 4A shows how tryptophan (loop
1) may interact with loop 2 and loop 3. These inter-
actions ... that contains one
domain and no disulfide bonds. Its stability in the native state may be
maintained mainly by key amino acids. In this study, two point-mutated
proteins each with a single base substitution...
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Tài liệu Báo cáo khoa học: Role of transcription factor activator protein 1 (AP1) in epidermal growth factor-mediated protection against apoptosis induced by a DNA-damaging agent doc
Ngày tải lên :
19/02/2014, 06:20
... and isola-
ted TAM1 and TAM2 cells, in either of which TAM67
was detected (Fig. 6A) . Because TAM67 lacks the
transactivation domain of c-Jun (amino acids 1–122),
but retains the DNA binding and ... expression of c-Fos and c-Jun, via the MAP
kinase signaling pathway.
TAM67 retains the DNA binding and leucine-zipper
region of c-Jun, but it lacks the transactivation domain
of c-Jun (amino acids 1–122). ... ADR-induced apop-
tosis, suggesting that EGF transmitted the antiapoptotic signal in such a
way that it activated AP1 via a MAP kinase signaling pathway. TMK-1
cells stably transfected with TAM67,...
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Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
Ngày tải lên :
22/02/2014, 04:20
... in cold adaptation
of an alkaline phosphatase
Konstantinos Mavromatis
1,
*, Iason Tsigos
2,
*, Maria Tzanodaskalaki
2
, Michael Kokkinidis
1,3
and Vassilis Bouriotis
1,2
1
Department of Biology, ... group of Ala261 side-chain could produce steric
clashes with the aromatic ring of Tyr269, and these
unfavorable interactions could lead to a decrease of local
flexibility and an increased E
a
value.
The ... mutant alkaline phosphatases.
(A) Temperature dependence of k
cat
of TAB5 (d), mutants G26 1A
(r), G26 1A/ Y26 9A (j)andE.coli (·) alkaline phosphatases at
temperature range 5–25 °C. k
cat
values...
- 6
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- 0
Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... amplication
betweenC7UandC8L.Primersinitalicswereusedinthegenotyping
of allele M.
C1U
5Â GAG AGG AAC TGA ACA GAA CAT TG 3Â
C1L 5Â CAA CTG CGT ATT AGT GAA GAA TG 3Â
C2U 5Â AAT CAA ATT TTA TTA TAA GAC C 3Â
C2L
5Â AAT AGC TAA TTA GAG ACC AT 3Â
C3U 5Â GGT GTC AAA ... 5Â ATA CAC ACA AAC TCA CAA GG 3Â
MWU 5Â CAA CAT ATT TTA AAT AAA ATT GAC AAT 3Â
C9LM* 5Â ATA AAA ATG GTA TAC CTC ACT TGT*C 3Â
C9UM1 5Â TAA CAA TGA TTC TCT TTC TTT TAG 3Â
C9LM1 5Â AAT CTT TAT TTT ... TGG CAC AAC ATT GTA CAT TCT TGG G 3Â
C9U 5Â GTA TGG AAG TGT GGA ATA GTT T 3Â
C9L 5Â GGA CAC CAC AGA TAT CCA ATA G 3Â
C1011U 5Â CAT AAA ACT AAC AAT ACA TGT 3Â
C1011L 5Â TAG CAG ATA TTG AAA AGG AG...
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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf
Ngày tải lên :
07/03/2014, 03:20
... lg of DNA per 100 mm dish and
2 lg of DNA per 30 mm dish. The ratio of DNA coding
donor to DNA coding acceptor was 1 : 1 or 1 : 2.
Membrane preparation and radioligand
binding assay
For binding ... the
arginine-rich epitope (217RRRRKR222) of the third intra-
cellular loop were exchanged (D
2
R1: 217AARRKR222,
D
2
R2: 217AAAAKR222, D
2
R3: 217AAAAAA222), as well
as one variant of the dopamine ... density of the D
1
MUT (404AA405) was
Table 1. Binding parameters for the dopamine receptors. For dopa-
mine D
2
receptor binding, the statistical significance was evaluated
using a one-way ANOVA,...
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Báo cáo khoa học: Role of different moieties from the lipooligosaccharide molecule in biological activities of the Moraxella catarrhalis outer membrane pot
Ngày tải lên :
07/03/2014, 05:20
... guxx@nidcd.nih.gov
Present addresses
*School of Veterinary Medicine,
Yangzhou University, Yangzhou,
Jiangsu, China
†Defence Research and Development
Canada-Suffield, Medicine Hat, Alberta,
Canada
Database
The nucleotide ... were
obtained via 3070xl DNA analyzer (Applied Biosystems,
Foster City, CA) and analyzed with dnastar software
(DNASTAR Inc., Madison, WI).
Transposon mutagenesis and identification
of lgt3 gene
In ... Hansen for providing strain O35E,
Wenzhou Hong for assisting in the animal challenge,
and Robert Morell and Yandan Yang for helping in
DNA sequencing. This research was supported by the
Intramural...
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Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
Ngày tải lên :
07/03/2014, 11:20
... of
A
DNA binding domain
B Ligand binding domain
Fig. 1. Sequence alignment. (A) Alignment
of DNA binding domain (C domain) and its
C-terminal extension. (B) Alignment of ligand
binding domain ... gene assay in in vivo results.
Experimental procedures
Parasites
The NMRI strain of S. mansoni was maintained in snails
(Biomphalaria glabrata) and Syrian golden hamsters
(Mesocricetus auratus ... Yeast one and two-hybrid assays. (A) Yeast one hybrid
assay showing that SmNR1 contains an autonomous transactivation
function in A ⁄ B domain. Individual AH109 yeast colonies obtained
from an...
- 16
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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx
Ngày tải lên :
07/03/2014, 12:20
... site and plays a
critical role in substrate binding [41]. Substrate or sub-
strate analogues may anchor this loop upon binding,
making a substantial conformational change compared
to the apo structure.
Table ... B, Sawka-Dobrowolska W, Gancarz
R, Kucharska-Zon
˜
M, Latajka R, Amrhein N, Miziak
P & Szczepanik W (2004) Experimental and ab initio
calculated structures of 2-aminoindane-2-phosphonic
acid, ... Molecular
phenotyping of the PAL1 and PAL2 mutants of Arabid-
opsis thaliana reveals far-reaching consequences on
phenylpropanoid, amino acid, and carbohydrate meta-
bolism. Plant Cell 16, 2749–2771.
Tyr-loop in...
- 16
- 502
- 0
Báo cáo khoa học: Identification of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx
Ngày tải lên :
08/03/2014, 02:20
... presence of an additional phosphate group (1032), and additional PEtn moiety (1075), an additional phosphate group and an additional PEtn moiety (1155) and one additional phosphate group and
two additional ... 227–
244.
24. Aspinall, G.O., Monteiro, M .A. & Pang, H. (1995) Lipo-oligo-
saccharide of Campylobacter lari type strain ATCC 35221.
Structure of the liberated oligosaccharide and an associated
extracellular ... The remaining resolved residues in the
a- anomeric region at 5.07 and 5.39 p.p.m. and a minor
signal at 5.50 p.p.m. were determined to be gluco-
pyranose amino sugars, from the appearance of their
spin...
- 8
- 361
- 1
Báo cáo khoa học: Identification of a novel binding site for calmodulin in ammodytoxin A, a neurotoxic group IIA phospholipase A2 docx
Ngày tải lên :
17/03/2014, 03:20
... G
GAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC
3+
wt
GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC
3+
YIRN
GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG
3– GGAATTCTGCAGTTAGCATTTgagCTCtccCTTGCACAAgAAGTCCGG
Ó FEBS 2003 A novel ... GGGCAAgcttgCTaTTcCCTCCTACTCCTcTTACGGATGCTACTGCGGCtgGGGGGG
2a+ GACTGCAaCCCCAAAtCGGACAG
2b+ CAAATACaAgCGGGtGAACGGGGCTATCGTCTGTGaAAAAGGC
2 G
GAATTCGCgGCcGCCtTGTCACACTCACAAATCCGATTCTC
3+
wt
GGGCAAGCTTGCgGCcGCAATCTGCTTTCGAcAGAATCTGAAcACATAttcgAAAAAGTATATGC
3+
YIRN
GGGCAAGCTTGCgGCcGCAATCTGCTTCCGAcAGAATCTGAAcACATACAgCTATATATATAGG
3– ... study, interaction of group IIA
sPLA
2
toxins with CaM was evaluated using a set of AtxA
mutants, including this quadruple (KKML) mutant, as well
as recombinant DPLA
2
and its quadruple reciprocal
(YIRN)...
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Báo cáo khoa học: Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids Kohsuke Honda, Michihiko Kataoka, Eiji Sakuradani and Sakayu Shimizu doc
Ngày tải lên :
17/03/2014, 09:20
... (GAGCTGAATTCTTTGGTCA) containing an
EcoRI site (in italics), and dch-II (ATGTAAGATCTGA
ACTGGACTTCG) containing a BglII site (in italics),
cloned into pT7Blue, excised with EcoRI and BglII, and
then ... primer, GGATCCGGACCAGTTGGT
GATTTT, and antisense primer, GGATCCTTAGAAAA
ACTCATCGAGC (BamHI recognition are shown in
italics). The amplified Km
R
was excised with BamHI and
then inserted into the ... PCR using pADH10 and
the following two oligonucleotides as a template and as
primers, respectively. The sense primer (CAGGAGA
ATTCAAAATGGG) contained an EcoRI recognition site
(in italics), and...
- 9
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- 0
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