... họ gen avrBs1 chỉ tìm thấy ở Xanthomonas campestis pv. campestris, còn họ gen avrBs3/pth xuất hiện phổ biến ở loài Xanthomonasoryzae pv. oryzae [21]. Ở loài Xanthomonas axonopodis pv. citri ... l a trồng và l a hoang. Mười một trong số ñó là gen lặn bao gồm: xa5, xa5(t), xa8, xa13, xa15, xa19, xa20, xa24, xa28, xa31 và xa32. Có 6 gen ñã ñược tách dòng là: Xa1, xa5, xa13, Xa21 Xa26 ... IRBB54 (xa5+Xa21); IRBB55 (xa13+Xa21); - Bốn dòng mang 3 gen kháng là: IRBB59 (xa5+xa13+Xa21); IRBB61 (Xa4+xa5+Xa7); IRBB62 (Xa4+Xa7+Xa21); IRBB63 (xa5+Xa7+xa13); - Hai dòng mang 4 gen kháng...
... Keywords: Isolation, multiplex PCR, specific primer, Xanthomonasoryzae pv. oryzae Title: Isolation and identification of bacteria caught bacterial leaf blight (Xanthomonas oryzae pv. oryzae ) by ... Xanthomonasoryzae pv. oryzae. The pair of primers XO290R-F was designed to amplify a 290bp fragment based on rhs gene family of Xanthomonasoryzae pv. oryzae because these genes are structurally ... A THÀNH PHẦN Nguyễn Thị Liên1, Trần Thị Xuân Mai1 và Nguyễn Thị Pha1 ABTRACT Bacterial leaf blight of rice, caused by Xanthomonasoryzae pv. oryzae, is a harmful disease of rice plants...
... possible combinations: (B ¼ b-Rha, A ¼ a- Rha, A ¼ a- Fuc3NAc (1fi2) a- Rha)B A, B– A B A A, B A A, B A A, B A A B AA A, B AA A, B AA A, B AA A, B AA A, B– A A A, B AA A, B AA A Several of these ... modelled were AA B AA B A A, AA B A A B A A, AA B A AvB AA and A A B– A A B AA (B: b-Rhap ,A: a-Rhap, A: a- Fucp3NAc(1fi2 )a- Rhap). The phi and psi angles aredefined by H1–C1–O1–CX and C1–O1–CX–HX, ... away towardsthe terminal end. The following fragments were assignable(the assigned a- Rha being bold):B A B A, B A B– A, B AA A, B AA A, B AA A/ B, AA A, AA B, A A B, B A B, B A A, B A A, ...
... toan open reading frame (ORF) of EhPGDH was amplifiedby PCR using a cDNA library [26] as a template, andoligonucleotide primers (5¢-caGGATCCaagatagttgtgataaccga-3¢ and 5¢-caCTCGAGttagaacttattgacttggaa-3¢), ... protozoan parasite Entamoeba histolytica. Mol. Biochem.Parasitol. 97, 33–44.27. Nozaki, T., Asai, T., Sanchez, L.B., Kobayashi, S., Nakazawa, M.& Takeuchi, T. (1999) Characterization of ... methionine c-lyase from Entamoeba histolytica: a key enzyme ofsulfur-amino acid degradation in an anaerobic parasitic protistthat lacks forward and reverse transsulfuration pathways. J. Biol.Chem....
... clearly showed thatthe majority of the material constituted of a tetrasaccha-ride and a smaller amount of a tetrasaccharide-ribitol.The data shows that the AAT residue is indeed anacetamido-amino ... was clear that the oligosaccharidefraction was a mixture and that it was the same mixture asthat obtained from pneumococcal C-polysaccharide whentreated with aqueous 48% HF under same conditions. ... Thephosphoethanolamine and phosphate groups were absentas a result of that all phosphate ester linkages were broken.From the1H-NMR spectrum it was clear that the fractioncontained a major and a minor...
... CAGGCTCGTGGTGCTAAATGCCCGAACTGCCTGTGCTGTG3f GTAAGTACGGCTTCTGCGGTTCTGGTGACGCTTACTGTGG4f CGCTGGTTCTTGCCAGTCTCAGTGCCGTGGTTGCTAGGGAT1r TTTAGCACCACGAGCCTGGTCACCGCAACGCTGAGC2r CGCAGAAGCCGTACTTACCACAGCACAGGCAGTTCG3r ... CGCAGAAGCCGTACTTACCACAGCACAGGCAGTTCG3r GACTGGCAAGAACCAGCGCCACAGTAAGCGTCACCAReverse GCTAGGATCCCTAGCAACCACGGCACTable 2. Antifungal activity of WAMP- 1a. IC50is the concentrationnecessary for 50% growth inhibition.Fungi ... kiharae seeds with aunique 10-cysteine motifTatyana I. Odintsova1, Alexander A. Vassilevski2, Anna A. Slavokhotova1,Alexander K. Musolyamov2, Ekaterina I. Finkina2, Natalia V. Khadeeva1,...
... ofcarotenoids.AbbreviationsCCD, carotenoid cleavage dioxygenase; GST, glutathione S-transferase; NIST, National Institute of Standards and Technology; OsCCD1,Oryza sativa carotenoid cleavage ... Dun EA, Pillot JP, Letisse F, Matusova R, DanounS, Portais JC et al. (2008) Strigolactone inhibition ofshoot branching. Nature 455, 189–194.11 Umehara M, Hanada A, Yoshida S, Akiyama K, AriteT, ... 3-OH-c-car-otene and lycopene. The values represent ratios of the C17and C19dialdehydes in the sum of their peak areas calculated by integratingeach peak at its individual kmax. Data represent...
... molecular mass of118 kDa and a pI of 4.85, displayed a multidomain organ-ization bearing a canonical family 48 catalytic domain, a bacterial type 3a cellulose-binding module, and a putativefibronectin-III ... cellulase among BacillalesMarta M. Sa´nchez, F. I. Javier Pastor and Pilar DiazDepartment of Microbiology, Faculty of Biology, University of Barcelona, SpainSequence analysis of a Paenibacillus ... domain. The cloned cellulase, unique amongBacillales and designated Cel48C, was purified through a nity chromatography using its ability to bind Avicel.Maximum activity was achieved at 45 °C and...
... inositolphosphate, calcium, and tyrosine kinase signalingpathways. Proc Natl Acad Sci USA 91, 38–42.44 Hundt M, Tabata H, Jeon MS, Hayashi K, Tanaka Y,Krishna R, De Giorgio L, Liu YC, Fukata M &Altman ... signaling, andultimately, mammalian target of rapamycin (mTOR)-dependent translation of select mRNA ([24] andunpublished data) (Fig. 3). In particular, IL-2R signal-ing leads to Akt and mTOR activation, ... indysregulated differentiation. Acta Haematol 122 , 230–237.64 Nakamichi S, Senga Y, Inoue H, Emi A, Matsuki Y,Watanabe E, Hiramatsu R, Ogawa W & Kasuga M(2009) Role of the E3 ubiquitin ligase...
... withprotparam (http://www.expasy.org).Removal of the His-tag A factor Xa cleavage site was created between the lastamino acid (valine) and the His-tag. Cleavage by factor Xaoccurs after an arginine, ... Havukainen H, Haataja S, Kauko A, Pulliainen AT,Salminen A, Haikarainen T, Finne J & PapageorgiouAC (2008) Structural basis of the zinc- and terbium-mediated inhibition of ferroxidase activity ... at 25 °C in air-equilibrated 50 mmMops (pH 7.0). The software datlab 4.2, furnished by themanufacturers, was used for data acquisition and analysis.Analytical ultracentrifugationSedimentation...
... was amplified by PCR from theplasmid pCY167 (Suzuki H & Yamada C, Unpublished),using forward primer 5¢-CATATGGATGAGTACAAACAAGTAGATG-3¢ and reverse primer 5¢-GGATCCTCGAGCTCATTTACGTTTTAAATTAATGCCGAT-3¢ ... 2433–2439.11 Wada K, Hiratake J, Irie M, Okada T, Yamada C,Kumagai H, Suzuki H & Fukuyama K (2008) Crystalstructures of Escherichia coli c-glutamyltranspeptidasein complex with azaserine and acivicin: ... metabolism, and its reaction intermediate.Proc Natl Acad Sci USA 103, 6471–6476.9 Boanca G, Sand A, Okada T, Suzuki H, Kumagai H,Fukuyama K & Barycki JJ (2007) Autoprocessing ofHelicobacter...
... reverse-transcribed and PCR-amplified using proofreading DNApolymerase (Invitrogen), forward primer 5¢-TTCCTGCAGTGGAAACCGGCAGTGAGGCCA-3¢ and reverseprimer 5¢-CGGAAAACCATCGCTAACAACTAAGCTTGGG-3¢. ... molecu-lar mass of the a- chain from a purified sample wasalso similar to that of human a2 (Fig. 4A) . Third,by putative amino acid sequence alignment, the deer a- chain contains a tandem repeat that ... Putative amino acid sequence analysis and divergence of mammal Hps. (A, B) Amino acid sequence alignment of the a- and b-chainsof human and deer. Variable regions are shaded in black. The cDNA...