resident and infiltrating immune cells
Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
Ngày tải lên :
30/03/2014, 15:20
... hydrolase promoter in
human immune cells and neuronal cells by leptin and progesterone
Mauro Maccarrone
1,2
, Valeria Gasperi
3
, Filomena Fezza
1
, Alessandro Finazzi-Agro
`
3
and Antonello Rossi
3
1
Department ... seems noteworthy that leptin [42,43] and
progesterone [44,45] play roles in survival, death and
proliferation of immune and neuronal cells; processes in
which AEA and the endocannabinoid system are ... of AMT, NAT and PLD in the
same cells (Figs 1A,B,D and data not shown). It should be
recalled that also CHP100 cells are d evoid o f c annabinoid
receptors [18].
Both U937 and CHP100 cells have...
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- 0
Báo cáo y học: " Effect of Weight Reduction on Cardiovascular Risk Factors and CD34-positive Cells in Circulatio"
Ngày tải lên :
25/10/2012, 10:51
... of cells after the diet to the percentage
of cells before the diet) and the percentage of body fat
lost by each subject during the study.
A correlation also exists between CD34+ cells
and ... weight adults, and the colony-forming capac-
ity of these cells is blunted [17, 18]. Alterations in en-
dothelial cells and EPC function associated with obe-
sity precede atherosclerosis and thrombosis ... blood–derived cells from which endothelial
cells in culture were developed were described as
cells expressing CD34 antigen. It has been hypothe-
sized that endothelial progenitor cells and hemato-
poietic...
- 8
- 594
- 0
Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-specific blood vascular and lymphatic endothelial cells of the mouse pdf
Ngày tải lên :
18/02/2014, 17:20
... dishes and cultured at 33 °C (day 0 in Fig. 1).
For the initial 2 weeks, primary cells, including both
tsA58T Ag-negative cells (primarily fibroblasts) and
tsA58T Ag-positive cells, proliferated, and ... isolation and
maintenance of murine endothelial cells from various
developmental stages and locations is important for
dissecting molecular and cellular mechanisms of endo-
thelial development and ... in these cells. Mes-
enteric BECs and LECs (Fig. 5A,B) were also obtained
by the same strategy as illustrated in Figs 1 and 4, and
were treated with VEGF-A or VEGF-C (Fig. 5C).
MAPK and Akt phosphorylation...
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- 0
Tài liệu Báo cáo khoa học: A peptide containing a novel FPGN CD40-binding sequence enhances adenoviral infection of murine and human dendritic cells doc
Ngày tải lên :
20/02/2014, 11:20
... detectable
homology to CD40 ligand (data not shown) and that
enhances adenoviral infection of CD40-positive cells of both
human and murine origin, including DC and B cells
resistant to infection ... as human and murine B cells. DC,LCL,L10A,Blasts:theselabels
refer, respectively, to day 8 human DC, EBV-immortalized human B
cells, murine B lymphoma L10A cells and murine LPS blasts. Cells
(10
5
) ... that
binds to CD40 and enhances adenoviral infection of mouse
and human DC and B lineage cells.
Materials and methods
Cell lines
Epstein–Barr virus- (EBV-) transformed human B cells were
provided...
- 8
- 458
- 0
Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer pdf
Ngày tải lên :
21/02/2014, 00:20
... dopamine,
R-(–)propylnorapomorphine, and raclopride did not affect
oligomerization of D
2L
and D
2S
in Sf9 and HEK293 cells.
Human D
2
dopamine receptors can therefore form consti-
tutive oligomers in Sf9 cells and in HEK293 cells ... the dopamine
D
2L
and D
2S
receptors can form constitutive homo- and
hetero-oligomers in two expression systems (Sf9 and
HEK293 cells) and these are not regulated by receptor
ligands. Our study ... can
also form hetero-oligomers in Sf9 cells. Time-resolved
FRET with europium and XL665-labelled antibodies was
appliedtowholeSf9 cellsandtomembranesfromSf9 cells
expressing epitope-tagged D
2
receptors....
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- 618
- 0
Tài liệu Báo cáo khoa học: Infrared spectroscopy as a tool for discrimination between sensitive and multiresistant K562 cells doc
Ngày tải lên :
21/02/2014, 03:20
... to the asymmetric stretching of CH
3
,and
the band at 2 873 cm
)1
to its symmetric mode. T he bands at
2926 and 2853 cm
)1
can be a ssigned to an asymmetric and
symmetric stretching mode of CH
2
, ... between 1300 and 900 cm
)1
arise mainly
from phosphate associated with nucleic acids, i.e. DNA
and RNA. The a bsorption bands a t 1245 a nd 1087 cm
)1
are c haracteristic o f asymmetric and symmetric ... plot of PCA-reduced s pectra of K5 62 cells.
Resistant K562 cells (39 spectra, black stars), sensitive K562 cells
(39 sp ectra, circles) o f the training (full) and test s et (empty). The
percentage...
- 6
- 555
- 0
Control of Innate and Adaptive Immune Responses during Infectious Diseases ppt
Ngày tải lên :
05/03/2014, 23:20
... 1626–9.
12
J. Aliberti
NK cells, JG CD3
+
T cells, noncytolytic NK cells, lymphoid tissue inducer cells, and
skin-homing IL-13
+
T cells. The receptor for IL-22 is distinct and consists of IL-22R, ... transmission. On the other hand, hosts need a robust and extended
immune response in order to expand memory cells. This critical balance is where
the co-evolution between host and pathogens lies. This ... activates NK cells to produce IFN-J and
drives the proliferation of type 1 CD4
+
and CD8
+
T cells, which produce even more
IFN-J. IFN-J-producing cells is a central component to induce and maintain...
- 189
- 1.4K
- 1
Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
Ngày tải lên :
18/03/2014, 01:20
... was detected in KM12,
KM20L2 and WiDr cells. COX-1 was highly expressed in
HT29 and WiDr cells and weakly expressed in KM20L2
cells. COX-2 was detected only in WiDr cells. The two
terminal PGE
2
-biosynthetic ... mast cells, human mast
cells developed in the presence of SCF and IL-6 from cord
blood cells [38] expressed only sPLA
2
-IID, but not the other
sPLA
2
s including -IB, -IIA, -IIE, -IIF, -V and ... by sPLA
2
-IIA and -V
(net 4–5%) (Fig. 3A).
When cells expressing sPLA
2
-IID were cocultured with
those coexpressing COX-2 and mPGES and then stimulated
(transcellular prostaglandin biosynthesis...
- 10
- 334
- 0
Báo cáo khoa học: Transport of the phosphonodipeptide alafosfalin by the H+/peptide cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells doc
Ngày tải lên :
23/03/2014, 12:20
... H
+
/peptide
cotransporters PEPT1 and PEPT2 in intestinal and renal epithelial cells
Jana Neumann
1
, Mandy Bruch
1
, Sabine Gebauer
2
and Matthias Brandsch
1
1
Membrane Transport Group, Biozentrum and
2
Institute ... inhibition constants and the competitive nature of
inhibition were confirmed in Dixon-type experiments. Caco-
2 cellsand SKPT cells were also cultured on permeable filters:
apical uptake and transepithelial ... plus tracer binding, of 19.2% and 5.1% were
determined with an excess amount of unlabeled Gly-Sar
(31.6 m
M
Gly-Sar for Caco-2 cells and 20 m
M
Gly-Sar for
SKPT cells) and subtracted from total uptake...
- 6
- 359
- 0
Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx
Ngày tải lên :
28/03/2014, 22:21
... conventional
CD4
+
and CD8
+
T cells, and normal or increased
development of CD4
+
and CD8
+
T cells, which have
an activated memory-cell-like phenotype ([8–12],
reviewed in [13] and [14]). These cells have ... devel-
opment and function of iNKT cells and cd T cells
seems to be quite different from conventional ab
T cells. This minireview focuses on this aspect of Itk,
its role in the development and function ... iNKT and
NKT-like cd T cells.
Itk and i NKT cell development
NKT cells are a subset of innate T cells characterized by
their expression of the NK1.1 marker along with the ab
TCR. Although these cells...
- 10
- 454
- 0
Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
Ngày tải lên :
31/03/2014, 07:20
... cord.
NPFF-related peptides from cells and tissues were extracted
and purified by RP-HPLC, assessed by radioimmunoassay
and identified by using on-line capillary HPLC/nanospray
ion trap tandem mass spectrometry ... at
94 °Cand30sat68°C. PCR products were cloned in
pEGFPn3 (Clontech) and sequenced on both strands.
Tissue extraction
Mouse spinal cord tissue. Animals were handled in
accordance with standard ... should help to understand their role
in neurotransmision. Thus, we investigated the processing of
proNPFF
A
in SH-SY5Y human neuroblastoma cells and
COS-7 (nonneuronal) cells transiently transfected...
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- 0
Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx
Ngày tải lên :
24/03/2014, 04:21
... between 48 and 60 h post-transfection. Blood
cells and cell lines were labeled with calcein-AM (5 mL per
10
8
cells) for 30 min at 37 8C. Red blood cells, mixed white
blood cells, Ramos and Daudi ... other blood cells
including CD281 cells and CD31 cells (data not shown).
The antibody was also used to probe Western blots of
extracts from several cell lines and purified peripheral blood
cells. The ... with
adherent transfected COS7 cells, a binding assay with
adherent endothelial cells was also performed. HUVEC
cells (ATCC) were plated and grown to confluence in
96-well plates. COS7 cells were transfected...
- 14
- 540
- 0
Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
Ngày tải lên :
25/10/2012, 11:18
... Cytb gene and Hp in-
duced mutations in D-Loop region were closely re-
lated to the bacterial virulence and the endogenous
ROS level.
Materials and metho ds
Cells and Hp Strains
AGS cells were ...
DHR-123 and 50 µmo l/ L H
2
O
2
for 24 h.
Detection of ROS using Flow cytometry
Cells were washed with PBS once. After trypsin
digestion, AGS cells were re-suspended in PBS and
1×10
4
viable cells ... for both the heavy and light strands of the
mtDNA, and contains essential transcription and rep -
lication elements. The D-loop region is a hot spot for
mtDNA alterations and the nucleotide...
- 12
- 557
- 2
- listening and speaking
- mode sensors and switches
- voltage supply and bu
- mechanisms and mechanical devices sourcebook
- hiv1 efficiently infects susceptible cells and causes aids in humans although hiv can also enter the cells of old world monkeys
- and often undergo chemical modifications of their base moieties as modified nucleotides may have deleterious effects on cells
- table views and cells
- and an increase in the presentation of the endogenous lectin galectin1 sensing these changes on the surface of p16 ink4a expressing pancreatic carcinoma cells capan1
- role of glial cells in the formation and maintenance of synapses
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