relationship between prostate specific antigen sex hormone binding globulin and androgen receptor cag repeat polymorphisms in subfertile and fertile men
Trinucleotide (CAG) repeat polymorphism of the androgen receptor gene in human disease
... E.L Yong (2001) Relationship between prostate- specific antigen, testosterone, sex- hormone binding globulin and androgen receptor CAG repeat polymorphisms in subfertile and normal men Molecular ... REPEAT POLYMORPHISMS IN SUBFERTILE AND FERTILE MEN INTRODUCTION 119 Androgenic parameters important for prostate cancer 120 1.1 Prostate specific antigen 120 1.2 Sex hormone- binding globulin 121 ... PROSTATESPECIFIC ANTIGEN, SEX HORMONE BINDING GLOBULIN AND ANDROGEN RECEPTOR CAG REPEAT POLYMORPHISM IN SUBFERTILE AND FERTILE MEN Table1 Descriptive statistics of the various parameters measured in the...
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Báo cáo Y học: Transient activation of the c-Jun N-terminal kinase (JNK) activity by ligation of the tetraspan CD53 antigen in different cell types pptx
... proteins containing the GAL4 DNAbinding domain and the c-Jun activation domain (residues 1–223) in wild-type and mutant forms (not phosphorylatable by mutation of both serines 63 and 73 to alanine) ... of T cells with CD2 augments TCR-CD3-mediated activation of protein tyrosine kinase p72syk, resulting in incresed tyrosine phosphorylation of adapter proteins, Shc and Cbl Int Immunol 10, 833–845 ... M.E (1997) A novel link between integrins, transmembrane-4-superfamily proteins (CD63 and CD81), and phosphatidylinositol 4-kinase J Biol Chem 272, 2595–2598 Chan, E.D., Winston, B.W., Uh, S.T.,...
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Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf
... VRK2B protein was more abundant in some cell lines, such as C4-I, HeLa, MCF7 or the colon carcinoma WiDr, and detected in smaller amounts in the remaining carcinoma cell lines (Fig 3A) In lymphoma ... protein family might play a role in proliferation because they are highly expressed in early haematopoietic development in murine embryos [18], in tissues containing proliferative cells and in ... signal and is detected in the nucleus in some cell lines and in transfected cells [11,13], but it is also present in the cytosol in other cell lines [15], particularly in some types of adenocarcinoma...
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báo cáo khoa học: " Identification of tissue-specific, abiotic stressresponsive gene expression patterns in wine grape (Vitis vinifera L.) based on curation and mining of large-scale EST data sets" docx
... processes including berry development in wine grape [30,45] and in table grape [46], tissue -specific gene expression [6,42], the fulfillment of chilling requirements in dormant grape buds [34], and ... “stress-responsive” (17%) including overrepresented transcripts encoding xyloglucan endotransglucosylase/ hydrolases, a DEAD box RNA helicase, and seed storage proteins including albumins and globulins and several ... duplication pattern as seen as the inchoate diagonals between plates and (pink) and between and (purple) in Figure 1E, and all four plates (purple) in Figure 1F In other cases, a plate did not match...
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Báo cáo y học: " Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology" pptx
... al R26.6 Figure and CG7236 minibrain show cell line -specific phenotypes CG7236 and minibrain show cell line -specific phenotypes (a) Silencing of the cdc2-related kinase CG7236 in S2R+ cells gives ... 5% bovine serum albumin in PBS-T for 15 minutes Cells were incubated with primary antibody (-Tubulin) in PBS containing 1% bovine serum albumin overnight at 4°C Cells were then washed and incubated ... according to kinase families Total Serine/threonine kinase Neuronal % Hemocyte % 146 20 13.7 22 15.1 Tyrosine kinase 49 10.2 12.2 Protein kinase 26 11.5 3.8 Lipid kinase 23 13.0 8.7 Guanylate kinase...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... HO-2 mRNA and protein and induced the expression of HO-1 mRNA and protein In contrast, the scrambled HO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins in HeLa and HepG2 ... cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma) YN-1 cells were maintained in Iscove’s modified Dulbecco’s medium (Sigma), and K562, KG1 and Jurkat cells were maintained in RPMI-1640 ... maintained in HO-2– ⁄ – mice through appropriate resetting of HO-1 expression In contrast with HO-1 expression, expression of HO-2 mRNA and protein was not increased in several human cell lines...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
... N39K–cystatin C, lacks inhibitory activity against the C13 family cysteine protease, legumain, because of removal of the key amino acid in the legumain -binding site of cystatin C [22] The amino acid ... cysteine proteinases, the cysteine protease inhibitory capacity of the cell lysate was determined by measuring the inhibition of (A) papain and (B) cathepsin B As positive and negative controls, instead ... (Fig 7B), carrying inactivating substitutions of key amino acid side chains important for target enzyme binding, (R9G,L9G, V10G,W106G)–cystatin C and N39K–cystatin C Both protein variants, which...
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Báo cáo khoa học: A pathway through interferon-c is the main pathway for induction of nitric oxide upon stimulation with bacterial lipopolysaccharide in mouse peritoneal cells pot
... results were obtained in two other independent experiments N.D., not determined at the 2-h time-point and maintained up to the 18-h timepoint (Fig 6A) Specificity of IRF-1 binding to the DNA probe ... Ig, of LPS-induced NO production in murine PEC but not in the RAW264.7 murine macrophage cell line Cells of murine PEC and of the RAW264.7 murine macrophage cell line were cultured and stimulated ... which lacks the binding ability owing to substitution of two AA residues in the IRF-1 binding motif to GG, and also because the bands were supershifted out of the range by preincubation of the...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... antinociceptive, neuroprotective and anti -in ammatory properties in different experimental models [30–35] Ebselen inhibits leukocyte in ltration and activation, leading to elimination of H2O2 in ... 1.11.1.6), and low molecular weight antioxidants, such as vitamins and various biological dyes, can reduce ROS in vivo [16–19] GPX is an important oxidative enzyme and a selenoprotein, incorporating ... selenocysteine residue at the active site [20–22] It catalyzes the reduction of H2O2, and a variety of organic hydroperoxides, resulting in effective elimination of various ROS in vivo and in vitro...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... establishment of doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression of the original ... selection and functional testing of individual clones In contrast, plasmids with an Epstein–Barr virus (EBV) origin of replication oriP in the presence of EBV-encoded EBNA-1 protein are continuously ... GAATTCTGCAGAT TCGA gccacc ATGCGGGA (polylinker in bold, Kozak motif in lower case, cDNA underlined); the 3¢ interface is ATTTCTAGA TCCAGCAC For pEBTetD ⁄ ETTh, the 5¢ interface is identical; the 3¢ interface...
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báo cáo hóa học:" Preclinical evaluation of dasatinib, a potent Src kinase inhibitor, in melanoma cell lines" pptx
... advanced melanoma and the reported relationship between Src kinase and melanoma progression, we examined the preclinical activity of Src inhibition, using dasatinib, alone and in combination with ... dasatinib in combination with epirubicin and taxotere were also examined in HT144 and Lox-IMVI (See additional file 3: Combination assays of dasatinib with epirubicin or taxotere) In both HT144 and ... Src kinase, in addition to BCR-Abl, c-KIT, PDGFR and ephrin-A receptor kinases It is the most potent Src kinase inhibitor currently in clinical development with an IC50 of 0.5 nM for Src kinase...
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Báo cáo sinh học: " Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met receptor in the RMS cell lines used in our experiments (RD, CW9019 and RH30), ... score was calculated from the staining intensity and percentage of positive staining cells The staining intensity was scored as (very weak), (weak), (moderate) and (intense) The positive rate score ... HE staining and IHC staining of myogenin and phospho-c-Met were shown Case is phospho-c-Met negative whereas case is phospho-c-Met positive Positive staining of phospho-c-Met was observed in both...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
... obtained with vemurafenib treatment, the blue line with metformin, and the red line with combination treatment b) Combination index for the combination of μM vemurafenib and 10 mM metformin of ... combination only in a subset of BRAFV600E mutant cell lines and none of the BRAF wild type cells Interestingly, single agent metformin treatment in the NRASQ61K mutant SKMEL173 resulted in increased ... stimulating the growth of one cell line in our panel, and the antagonistic effects of the combination of metformin and vemurafenib in several cell lines, the combination of vemurafenib and metformin...
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Báo cáo hóa học: " Expression of frog virus 3 genes is impaired in mammalian cell lines" pot
... expressed in mammalian cell lines However, essential viral genes must express in mammalian cell lines since the virus is able to infect and successfully replicate in many cell lines, including rodent, ... various time points post-infection, cells were fixed in 3.7% paraformaldehyde in phosphate buffer saline (PBS) for 10 minutes, and permeabilized in a 0.1% Triton X-100 solution for minutes Indirect ... with 10% fetal bovine serum (FBS; HyClone, Ottawa, ON), penicillin (100 U/mL) and streptomycin (100 g/mL) at 30°C BGMK and HeLa cells were obtained from ATCC and maintained in Dulbecco's modified...
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báo cáo hóa học:" Inhibition of phosphorylated c-Met in rhabdomyosarcoma cell lines by a small molecule inhibitor SU11274" pdf
... c-Met receptor coding sequence [28,30-32] There were no activating mutations in the tyrosine kinase region of the c-Met receptor in the RMS cell lines used in our experiments (RD, CW9019 and RH30), ... score was calculated from the staining intensity and percentage of positive staining cells The staining intensity was scored as (very weak), (weak), (moderate) and (intense) The positive rate score ... HE staining and IHC staining of myogenin and phospho-c-Met were shown Case is phospho-c-Met negative whereas case is phospho-c-Met positive Positive staining of phospho-c-Met was observed in both...
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Báo cáo y học: " DNA methylation patterns associate with genetic and gene expression variation in HapMap cell lines" ppt
... in CGIs, histone modifications, and TF binding sites 1.0 Methylation 0.8 0.6 0.4 0.2 0.0 In Out CGIs In Out H3K27ac In Out H3K4me3 In Out H3K9ac In Out TF binding sites Figure Distribution of ... reported in our study were also observed in human brain samples [5] These findings provide a framework to help the interpretation of GWAS findings and improve our understanding of the underlying biology ... and imprinting effects can be sexspecific [45], raising the possibility that some of the meQTLs may act in a sex- dependent manner However, we did not find compelling genome-wide significant sexspecific...
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báo cáo khoa học: "Effect of arginase II on L-arginine depletion and cell growth in murine cell lines of renal cell carcinoma" pptx
... HPLC after deproteinization with methanol and derivatization with OPA for (A) L-arginine and (B) L-ornithine and (C) L-glutamine Standards of L-arginine, L-ornithine and Lglutamine in methanol were ... Figure L-arginine, L-ornithine and L-glutamine levels L-arginine, L-ornithine and L-glutamine levels Tissue culture supernatants from CL-2, CL-19, and Renca cells were collected at 24, 48, and 72 ... different tissues, including kidney, brain, and prostate [6,9,10] Arginase I, is primarily involved in the detoxification of ammonia and urea synthesis, whereas arginase II is involved in the synthesis...
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top-antitop cross section measurement as a function of the jet multiplicity in the final state and beyond the standard model top-antitop resonances search at the atlas detector at cern
... understanding of the Standard Model, particularly the QCD interaction It also helps understanding and verifying the Standard Model description of this quark More information can be found in a review ... creating a “colour flow” connecting the partons and showering the partons in this colour flow independently The parton shower in this procedure uses transverse momentum ordering, instead of using ... numerically equal to its energy in its rest frame mc2 and its inverse Compton wavelength mc/ The Einstein summation convention of summing repeated indices in vectors and tensors is adopted Part...
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- the relationship between tree adjoining grammars
- relationship between data and information ppt
- relationship between data and information with examples
- the picture of dorian gray relationship between art and life
- relationship between reliability and test length
- relationship between power and energy
- relationship between power and energy physics
- relationship between power and energy equation
- state the relationship between energy power and time
- relationship between power energy and time
- relationship between molar mass and formula weight
- relationship between work power and time
- relationship between energy power and time
- approach to the patient with a prostate nodule or elevated prostate specific antigen level
- parathyroid hormone calcitonin calcium and phosphate metabolism vitamin d bone and teeth
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