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11/04/2014, 10:32
... residues, and finally, carboxyl i H R Bourne, D A Sanders, and F McCormick, Nature (London) 348, 125 (1990) H R Bourne, D A Sanders, and F McCormick, Nature (London) 349, 117 (1990) -~R Khosravi-Far and ... N-Ras, and lamin B, have C A A X boxes ending in methionine (CVIM, CI1M, CVVM, and CALM, respectively) The C A A X boxes of the transducin ,/ subunit and H-Ras end in serine (CVIS and CVLS), and ... containing 0.5% (w/v) sodium cholate and protease inhibitors, then briefly sonicated and recentrifuged The supernatants from the first and second centrifugation are combined and centrifuged at 100,000...