regulation of lectin production by the human pathogens pseudomonas aeruginosa and chromobacterium violaceum effects of choline trehalose and ethanol
The 11th form non-english majors’ level of satisfaction with their reading comprehension lessons at phan boi chau specialized upper secondary school, nghe an
... procedure of the study: At the beginning of the experiment, a pre-test was given to both groups The primary goal of the pre-test was to measure and compare the two groups in term of the students’ ... proficiency before and after the experiment Thus, the results of these tests were analyzes using the Excel Spread Program to calculate the Independent t-test 1.1 The results and discussion of the ... almost the same level of vocabulary ability at the onset of the experiment 1.2 The results and discussion of the post-test scores of the two groups - 28 - After weeks of the experiment, both...
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THE 11TH FORM NON ENGLISH MAJORS’ LEVEL OF SATISFACTION WITH THEIR READING COMPREHENSION LESSONS AT PHAN BOI CHAU SPECIALIZED UP
... French and Russian The school selects all the best teachers and students from school in the province The main duty of the school is to train and foster talents for the province and the country The ... suitable and relevant the teaching and learning styles are to the students’ interests, habits and learning ability…, the more they can get from the lesson Therefore, they are more satisfied with their ... since the students were in grade 10 They are different in age, teaching experience and qualification Two of them are 27 years old One of them is 30 and the rest is 34 years old Therefore, they...
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Tài liệu Báo cáo Y học: Interaction of decorin with CNBr peptides from collagens I and II Evidence for multiple binding sites and essential lysyl residues in collagen pot
... presence of chondroitin/dermatan sulfate PGs at the d and e bands in the gap zone of the fibrils formed by the quarter staggered array of type I collagen molecules, and the presence of keratan ... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of the ... On the contrary, no influence of detergents was found, as determined by the addition of 1% Triton to the binding solution To further characterize the nature of the interaction between decorin and...
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Báo cáo khoa học: Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia pdf
... lacked the N-terminal regions Arrowheads show the sites cleaved and the molecular masses of the rest of the cleaved peptides Arrows show the N-termini of the 35 kDa forms of annexin A1 and annexin ... (B) These two controls are shown as thin dotted lines in (C) and (D) The result obtained in the presence of dicalcin and all of the annexins (E) was compared with the calculated sum of each of the ... annexin A4 and annexin A5 to identify which annexins were in the fraction of the 38 kDa and the 35 kDa proteins Cloning of annexin cDNAs On the basis of the partial amino acid sequences of the proteolytic...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... represent the limited case of six triplet sequences, the specificity of the observed effects confirms the higher selectivity of DAPI for the minor-groove of the ATT-triplet with respect to all the other ... also supported by the observation that the first 10 bases of the GC-rich random sequence of the AAT- and ATT-templates are copied without any pausing in relation to the drug-free syntheses This provides ... attributed to the (ATT)10 region (located at the 5¢-end of the ATT-template) folding into a hairpin-like structure The double helix of the hairpin stem, stabilized by the binding of DAPI into the minor-groove...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... to 1% of the material used in the pulldown experiment The presence of the TAP-Vg1RBP band in the anti-Staufen blot is a result of the strong binding of secondary anti-rabbit serum to the protein-A ... pathway In the nucleus, Vg1 is recognized by the RNA-binding proteins Vg1RBP and hnRNP I [7,21]; the export of this complex into the cytoplasm is followed by a structural reorganization of the RNP ... should be part of one and the same complex; on the basis of the experimental results obtained in the present study, we cannot exclude the possibility that ePAB and FRGY2 are indeed part of different...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... that of the higher binding site by NMR, even if the sugar-binding ability of one of the two binding sites is much higher than that of the other However, the hemagglutinating activity of lectin ... particularly H4, and barely interacts with the region of H1 and the O-methyl group The crystal structure of EW29Ch with lactose showed that the O2, O3 and O4 atoms of the galactose residue of lactose ... describing the binding of one ligand molecule to a single protein site using the Solver function of 15 EXCEL 2002 The weighted average of the H and N chemical-shift changes of Gly122 given by Dav...
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Báo cáo Y học: Exploration of the diaphorase activity of neutrophil NADPH oxidase Critical assessment of the interaction of iodonitrotetrazolium with the oxidase redox components doc
... [13], either by measurement of the rate of production of O2– or the rate of O2 uptake INT diaphorase activity was assayed by the rate of reduction of INT into formazan in the presence of superoxide ... but rather as the product of reduction of O2 by INT, and the SOD-dependent inhibition of INT reduction appears to be an indirect effect On the other hand, the INT radical may generate by dismutation ... additions of INT (cf Fig 8), the difference spectra relative to the Soret band were recorded and the extent of reoxidation was assessed by the decrease of the peak of absorbancy by reference to a base-line...
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Báo cáo y học: "Direct interaction of immunoglobulins with synovial fibroblasts: a missing link in the pathogenesis of rheumatoid arthritis" potx
... transformation), that leads to alteration in their apoptotic response, the attachment of these cells to articular cartilage and subsequently to the degradation of the cartilage matrix [11, 13] This notion ... present research on the interaction of RA-IgG and RASFs as well as other recent data, however, may change the picture It has been reported by Huizinga and colleagues that in a cohort of patients with ... IL-16 in RASFs, the present research from Smith and Cruikshank extends the panel of signals involved in the regulation of IL-16 at least under pathologic conditions Although the study does not...
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báo cáo khoa học: " Gene expression profiling in susceptible interaction of grapevine with its fungal pathogen Eutypa lata: Extending MapMan ontology for grapevine" pdf
... advantage of using MapMan is no doubt the ease and speed of the analysis The visualization of the genes distribution among the phytohormones confirms the large involvement of genes linked to auxins and ... Figure and 2: an overview of ripening regulation and the phenylpropanoid pathway The representation of berry development regulation provided by MapMan immediately highlights the large number of genes ... analysis The general trend of these classes is the same as reported in Figure of [21], in which it is evident that all of them are prevalently induced from stage A to B and then mostly repressed The...
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Interaction of EcoRI with noncognate DNA sequences computational investigation of dynamics of protein water and DNA conformation
... “inheritance is based on the production of specific particles (“seeds”) by all parts of the body and transmission of these at the time of conception” [1] If this were the case, then children would ... showing the exclusion of water molecules at the interface of the protein and DNA during the formation of the specific complex (Adopted from [92]) binding [60] The structures of the specific and nonspecific ... the operon in 1961 [8] Their theory was based on their observations of induction of the lac gene The isolation and characterization of the lac repressor, a protein, and the discovery that it actually...
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Interaction of scribble with zonula occludens and intermediate filament proteins
... contribution of reagents but also his support during the course of my project and advice throughout the writing and revision of the publication of my work My candidature in the Institute of Molecular and ... promotes the hydrolysis of GTP to GDP by the intrinsic GTPase activity of the G-protein (Jaffe and Hall, 2005) The recruitment and activation of Cdc42 and Rac1 is vital for initiation of epithelial ... Conversely, the basal activity of the Scrib complex is antagonized by the Par and Crb complexes apically and therefore restricted to the basal domain As such, opposing functions of the Par-Crb and Scrib...
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Báo cáo khoa học: DNA mediated disassembly of hRad51 and hRad52 proteins and recruitment of hRad51 to ssDNA by hRad52 pot
... the formation of large complex in the presence of hRad51, hRad52 and ssDNA presence of hRad52 renders much better binding of hRad51 to ssDNA even at lower concentrations of the latter, thereby ... concentrations of DNA as described in (A) in the absence of any nucleotide cofactors and later subjected to centrifugation and the resulting pellet and supernatant were analysed by SDS PAGE followed by silver ... to study the effect of DNA on hydrodynamic radius (Rh) of hRad51 hRad51 (1 lM) was incubated with 0, 1, 3, and lM of oligo PUC+ (in the absence of any nucleotide cofactor) followed by the measurement...
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Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray
... elucidation of the splicing mechanism and the identification of the key amino acid residues involved in the scission and ligation of the peptide bonds have facilitated the molecular engineering of artificial ... rather than bond cleavage and resynthesis and is catalyzed entirely by the amino acid residues contained in the intein.[1] The biochemical mechanism of protein splicing includes the formation of ... ASN O Figure Mechanism of intein splicing at the C-terminal junction of the Ssp DnaB intein, with Asn as the last amino acid of the intein and Cys as the first residue of the target protein Self-cleavage...
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Báo cáo y học: " Antagonistic interaction of HIV-1 Vpr with Hsf-mediated cellular heat shock response and Hsp16 in fission yeast (Schizosaccharomyces pombe)" potx
... noticed in the examination of two Vpr mutations The R77Q and I74A mutants, which separate the apoptosis and G2 arrest induced by Vpr [24,54], showed no separation between the G2 induction and apoptosis ... arrest and cell killing, both in fission yeast and human cells ([27]; our unpublished data) However, the suppressive effect of yeast Hsp16 and human HSP27 on Vpr are not identical Overproduction of ... fluorescence (B) Comparison of the Hsp16 protein levels in the presence and absence of Vpr as shown by Western blot analysis The vpr or vpr’-expressing cells were collected at the same time as in panel...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... consequence of the mutagenic replacements Effects of the His120Asn and His145Asn mutations on the affinity of metal binding to arginase II To further examine the effects of the His120fiAsn and His145fiAsn ... coordinated by His120 and His145, which are equivalent to the histidines at position 101 and 126 in the sequence of arginase I [10] However, they are clearly differentiated by the consequences of their ... explained by electrostatic repulsion between the a-carboxyl group of the amino acid and the introduced aspartic residue at position 149 On the other hand, as agmatine lacks the a-carboxyl group, there...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... peak at 7.65 mL on the elution profile Increase of the temperature of incubation was accompanied by the simultaneous increase of the peak eluted at 7.65 mL and decrease of the peak eluted at 8.65 ... Increase of the time of heating at 43 °C leading to decrease of parameter A up to 2.2–2.3 was accompanied by further decrease of the initial rate of polymerization When parameter A was in the range of ... was sedimented Therefore, by measuring the quantity of actin in the pellet we were able to estimate the chaperone activity of HSP25 and its mutants In the absence of HSP25 about 90% of heated actin...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... interaction in the presence of mm ATP On the other hand, 2-oxoglutarate did not influence the GlnK–TnrA interaction, either alone, in the absence of divalent metals, or in combination with ATP and Mg2+ ... lower panels show the binding isotherm and the best-fit curve according to the three sequential binding sites model BIAcore analysis of the GS–TnrA interaction The results of the immunoprecipitation ... interact with the C-terminus of TnrA, and may play a role in the regulation of TnrA activity and its proteolysis [15] To test this assumption, various truncations of TnrA (lacking six, 20 and 35 amino...
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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
... Consequently, the molecular studies of the interaction of SAgs with their specifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to the development ... treatment of several pathologies and because of the potential use of the toxins as biological weapons Alteration of their MHC and TCR binding capacity by site directed mutagenesis could be useful in the ... species; reduction and alkylation, and mutation of the Cys implicated in the intermolecular disulfide bridge Analysis of the three-dimensional structure of SSA [31] to determine which of the remaining...
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Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... from H2O2 production during the autoxidation of dopamine following the addition of varying amounts of the diphenol into solutions of NaCl ⁄ Pi (pH 7.4) that lacked catalase (A and B) and those ... peroxidase activities, and the studies by Wood et al [20] showing the enzyme to be activated by low concentrations of H2O2 Unquestionably, the generation of H2O2 and other ROI during tyrosinase-mediated ... circumvented by the enzyme employing these molecules to metabolizing substrates A likely scenario for the production of these molecules involves the partial reduction of O2 caused by 2412 Fig...
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