receptor atrial natriuretic peptide nitric oxide synthase and estrogen receptor mrnas expression in rat
Báo cáo y học: "Dynamic compression counteracts IL-1β induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs" pdf
Ngày tải lên :
09/08/2014, 10:23
... demonstrated a role for the integrins in mediating the mechanotransduction process, including downregulation of NO and PGE2 release, both in the presence and absence of IL-1β, utilizing iNOS and ... protein kinase; UT, untreated IL-1β induced iNOS and COX-2 and inhibited aggrecan expression Figure illustrates the effects of IL-1β on the relative expression levels of iNOS, COX-2, aggrecan and ... Nelson AH, Barone FC, Kumar S: SB203580 inhibits p38 mitogenactivated protein kinase, nitric oxide production, and inducible nitric oxide synthase in bovine cartilage-derived chondrocytes J Immunol...
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Báo cáo y học: "Protective effects of total fraction of avocado/soybean unsaponifiables on the structural changes in experimental dog osteoarthritis: inhibition of nitric oxide synthase and matrix metalloproteinase-13" docx
Ngày tải lên :
09/08/2014, 14:20
... Higashiyama H, Hirata Y, Kanno K, Saito I, Miyasaka N: Nitric oxide production and inducible nitric oxide synthase expression in inflammatory arthritides J Clin Invest 1995, 96:2357-2363 41 McInnes IB, ... an alpha2delta ligand, reduces the development of experimental osteoarthritis by inhibiting metalloproteinases and inducible nitric oxide synthase gene expression and synthesis in cartilage chondrocytes ... cartilage in the postcontusive model of OA in rabbits [8] and to improve the subchondral bone structure in an ovine OA model induced by meniscectomy [9] In addition to the above findings, and most interesting...
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Báo cáo y học: "Aciculatin inhibits lipopolysaccharide-mediated inducible nitric oxide synthase and cyclooxygenase-2 expression via suppressing NF-B and JNK/p38 MAPK activation pathways" pot
Ngày tải lên :
10/08/2014, 05:21
... Signal 2001, 13:85-94 Goodwin DC, Landino LM, Marnett LJ: Effects of nitric oxide and nitric oxide- derived species on prostaglandin endoperoxide synthase and prostaglandin biosynthesis FASEB J 1999, ... According to our results, aciculatin inhibited LPS-induced NO and PGE2 production in a concentration-dependent manner by decreasing the expression of iNOS and COX-2 at both gene and protein level in ... assessed using the MTT assay (Figure 2C) Aciculatin Inhibits LPS-Induced iNOS and COX-2 Gene and Protein Expression We next to determine whether the inhibitory effect of aciculatin in NO and PGE...
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Báo cáo y học: "Hyaluronan modulates accumulation of hypoxiainducible factor-1 alpha, inducible nitric oxide synthase, and matrix metalloproteinase-3 in the synovium of rat adjuvant-induced arthritis model" potx
Ngày tải lên :
12/08/2014, 17:21
... macrophages, mainly in inflammatory infiltrate and invasive pannus of the inflamed synovial joint Synovial lining cells and some chondrocytes were also found to be positive for HIF-1a, iNOS, and MMP3 ... lubricating agent but its exogenous administration can suppress the expression of inflammatory cytokines, MMPs, and free oxygen radicals to reduce inflammation in a post-laminectomy rat model [20] and ... characterized by acute cytokineinduced inflammation [25] Owing to infiltration of the injured tissues by immune cells and responses, swelling is a major sign during acute inflammation and might also be...
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Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
Ngày tải lên :
07/03/2014, 05:20
... lines on the autoradiogram The origin was marked with an ink spot Argininosuccinate is the immediate precursor to arginine in the urea cycle, and is converted to arginine and fumarate by argininosuccinate ... arginine that mimics an NOS reaction This reaction, however, produces argininosuccinate, not citrulline, and requires fumarate, indicating that ASL is catalyzing the reaction Because argininosuccinate ... assay Citrulline Hydroxyarginine Argininosuccinate Ornithine Origin 1-D 2-D Fig Two-dimensional TLC analysis of reaction product A reaction with [14C]arginine, desalted protein extract and ADF was...
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Báo cáo khoa học: " Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl3-induced toxicity in the rat forebrain cortex" doc
Ngày tải lên :
07/08/2014, 23:22
... apparently involved in neurodegeneration by different mechanisms, including oxidative stress and activation of intracellular signaling mechanisms [19] Al affects NO production in the brain Al decreases ... AlCl3 administration; (4) L-NAME group (n = 10) - animals were -4 treated with L-NAME with single dose (1 × 10 g dissolved in saline) before saline administration Using a stereotaxic instrument ... prepared in 10% sulfosalicylic acid for GSH determination [1] The protein content in the rat brain homogenates (forebrain cortex, ipsi- and contralateral) was measured by the Lowry method using bovine...
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Báo cáo y học: " Overexpression of endothelial nitric oxide synthase suppresses features of allergic asthma in mice" potx
Ngày tải lên :
12/08/2014, 16:20
... placed in a whole-body chamber, and basal readings were obtained and averaged for Aerosolized saline, followed by increasing doubling concentrations (1.56 – 25 mg/ml saline) of methacholine, were ... GU: Randomised double-blind placebo-controlled study of the effect of inhibition of nitric oxide synthesis in bradykinin-induced asthma Lancet 1996, 348:374-377 Redington AE, Meng QH, Springall ... measured in lung tissue by the Larginine to L-citrulline conversion assay using a nitric oxide synthase assay kit (Calbiochem, La Jolla, CA, USA; cat no 482700) according to the manufacturer's instructions...
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Traumatic brain injury and the effects of diazepam, diltiazem, and MK-801 on GABA-A receptor subunit expression in rat hippocampus ppt
Ngày tải lên :
10/08/2014, 05:21
... GABAAR Increases in α1 mRNA and protein expression during development correspond to increases in BZ binding and altered zinc sensitivity [54], while reduced α1 mRNA and protein expression in the ... NMDA receptors, increasing [Ca2+]i and subsequently activating CaMKII, resulting in altered GABAAR function due to phosphorylation of receptor proteins Page of 11 Although there is in vitro and indirect ... in an incomplete understanding of their contribution to injury-induced pathology, but have indicated that the receptor is affected by injury Sihver et al [12] found a decrease in GABAAR binding...
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Báo cáo y học: "Atrial natriuretic peptide infusion and nitric oxide inhalation in patients with acute respiratory distress syndrome" ppt
Ngày tải lên :
12/08/2014, 18:21
... S: Atrial natriuretic peptide in acute hypoxia-induced pulmonary hypertension in rats J Appl Physiol 1991, 71:807–814 Ljusegren ME, Andersson RG: Hypoxia induces release of atrial natriuretic peptide ... arterial and mixed venous concentrations of cGMP increased significantly during NO inhalation EVLWI did not change either during ANP infusion or during NO inhalation In patients who responded to NO inhalation ... two indicators: ice-cold water and indocyanin green (ICG) Cold distributes to both intravascular and extravascular volumes, whereas ICG remains intravascular Both indicators are injected into...
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Báo cáo khoa học: Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy ppt
Ngày tải lên :
07/03/2014, 03:20
... single-transmembrane polypeptide, each containing an extracellular ANPbinding domain (ECD), a transmembrane domain, and an intracellular domain consisting of an ATP-binding regulatory domain and ... Grammer RT, Rigby JW & Inagami T (1985) Photoaffinity labeling of atrial natriuretic factor Natriuretic peptide receptor signaling mechanism 21 22 23 24 25 receptor in bovine and rat adrenal cortical ... coordinates C-type natriuretic peptide and mitogen-signaling pathways in fibroblasts J Biol Chem 274, 4293–4299 Suenobu N, Shichiri M, Iwashina M, Marumo F & Hirata Y (1999) Natriuretic peptides and...
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Báo cáo sinh học: " Plasmid-encoded NP73-102 modulates atrial natriuretic peptide receptor signaling and plays a critical role in inducing tolerogenic dendritic cells" pdf
Ngày tải lên :
14/08/2014, 19:22
... The natriuretic peptide family comprises atrial natriuretic peptide, ANP, brain natriuretic peptide, BNP, C-type natriuretic peptide, CNP, Dendroaspis natriuretic peptide, DNP, and urodilatin ... Toll-like receptor- 2 (TLR2) and suppressor of cytokine synthesis-3 (SOCS3) are key players in integrating NPRA signaling with innate immunity and in the induction of tolerogenic DCs Materials and methods ... NPRA signaling on innate and adaptive immunity occur through NPRA-mediated alterations in gene expression in DCs Little is known about the role of NPRA signaling in innate immunity and about the...
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Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf
Ngày tải lên :
18/02/2014, 11:20
... in place of the flavoprotein, and then redox cycles within NOS; and (d) heme–NO binding is an intrinsic feature of catalysis that constrains the rate of heme reduction by the flavoprotein domain ... AI, autoinhibitory insert; B2R, bradykinin receptor B2; CaM, calmodulin; CT, C-terminal tail; HSP-90, heat-shock protein 90; iNOS, inducible nitric oxide synthase; ND, not determined; NA, not applicable; ... The kinetics of interflavin electron transfer (FAD and FMN) in dual-flavin enzymes has been studied using a T-jump method [79] and by observing rates of flavin semiquinone formation (FADH• and ⁄...
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Báo cáo khoa học: Space, time and nitric oxide – neuronal nitric oxide synthase generates signal pulses pptx
Ngày tải lên :
07/03/2014, 00:20
... were initiated by mixing 3–6 lm solutions of nNOS, mm arginine and 200 lm NADPH in air-saturated BTP at pH 7.5 with 120 lm calmodulin and mm CaCl2, or by mixing lm solutions of nNOS, mm arginine, ... nitric oxide synthase indicating a neural role for nitric oxide Nature 347, 768–770 Knowles RG, Merrett M, Salter M & Moncada S (1990) Differential induction of brain, lung and liver nitric oxide ... Structure of nitric oxide synthase oxygenase dimer with pterin and substrate Science 279, 2121–2126 36 Ghosh DK & Salerno JC (2003) Nitric oxide synthases: domain structure and alignment in enzyme...
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Báo cáo khoa học: Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs potx
Ngày tải lên :
16/03/2014, 13:20
... CaM-binding peptide reported in [21] Amino acids in bold represent conserved residues in the respective CaM-binding domains CaM-binding domain CaM amino acids in contact with eNOS peptide eNOS iNOS ... dichroism and 1H NMR studies on the structures of peptides derived from the calmodulin-binding domains of inducible and endothelial nitric- oxide synthase in solution and in complex with calmodulin J ... multidomain C-terminal reductase region and an N-terminal oxygenase domain The reductase domains bind NADPH, FAD, and FMN, and the oxygenase domain contains binding sites for heme, tetrahydrobiopterin...
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Báo cáo khoa học: Relationship between the structure of guanidines and N-hydroxyguanidines, their binding to inducible nitric oxide synthase (iNOS) and their iNOS-catalysed oxidation to NO pptx
Ngày tải lên :
16/03/2014, 22:20
... domain of inducible nitric- oxide synthase identifies a glutamate involved in arginine binding Biochemistry 36, 5097–5103 McMillan K & Masters BS (1995) Prokaryotic expression of the heme- and flavin-binding ... to the binding of l-Arg [10–14] and NOHA [10], nothing is known about the thermodynamics and kinetics of the binding of guanidines and N-hydroxyguanidines to iNOS Removal of the a-amino or a-carboxylate ... 0.4 binding of an N-hydroxyguanidine moiety in the iNOS heme pocket thus roughly involves the same binding energy as binding of the guanidine moiety, and the equilibrium constants are mainly...
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Báo cáo khoa học: Atrial natriuretic peptide-dependent photolabeling of a regulatory ATP-binding site on the natriuretic peptide receptor-A pptx
Ngày tải lên :
16/03/2014, 23:20
... were incubated in the dark at 37 °C for 12 in the presence of theophylline, IBMX, creatine phosphate, creatine kinase, GTP, 0.1 lM atrial natriuretic peptide (ANP) and MgCl2, as described in the ... Photolabeling competition experiments suggest that binding of ATP to the KHD is a highly cooperative event Interestingly, GC and ANP-binding studies using 8-azido-App(NH)p gave surprising results, reminiscent ... the guanylin peptides and heat-stable enterotoxin Bhandari et al showed that binding of an antibody raised against the KHD domain of GC-C was reduced when receptor was preincubated in the presence...
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Báo cáo khoa học: Rapamycin inhibits lipopolysaccharide induction of granulocyte-colony stimulating factor and inducible nitric oxide synthase expression in macrophages by reducing the levels of octamer-binding factor-2 doc
Ngày tải lên :
22/03/2014, 17:20
... expression of iNOS and G-CSF protein and mRNA were evaluated in macrophages, and the involvement of Oct-2 in LPS ⁄ mTOR-induced iNOS and G-CSF expression was investigated further Results Rapamycin inhibits ... G-CSF expression in response to LPS Oct-2 is directly involved in LPS-induced iNOS and G-CSF expression in RAW264.7 macrophages To further examine the involvement of Oct-2 in LPSinduced iNOS and ... inhibits LPS-induced G-CSF and iNOS expression G-CSF promoter iNOS promoter Oct-2 Oct-2 IgG A β-actin Input LPS LY Akt -in Rapa LPS Rapa Fig Rapamycin inhibits the binding of Oct-2 to the iNOS and G-CSF...
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Báo cáo khoa học: Cupiennin 1a, an antimicrobial peptide from the venom of the neotropical wandering spider Cupiennius salei, also inhibits the formation of nitric oxide by neuronal nitric oxide synthase pptx
Ngày tải lên :
23/03/2014, 09:21
... sequence, including the C-terminal and N-terminal domains This means that a substantial change in conformation occurs for the regulatory protein upon binding of cupiennin 1a to Ca2+-CaM, indicating ... and l-arginine; (b) a C-terminal reductase domain that binds FMN, FAD and NADPH; and (c) an intervening CaM-binding region that regulates electronic communication between the oxygenase and reductase ... features in common with certain amphibian peptides that inhibit the formation of NO by neuronal nitric oxide synthase (nNOS) [10] These particular amphibian peptides (e.g the caerins and splendipherin...
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Báo cáo khoa học: Homologous desensitization of guanylyl cyclase A, the receptor for atrial natriuretic peptide, is associated with a complex phosphorylation pattern pot
Ngày tải lên :
29/03/2014, 09:20
... membrane-spanning region (21 aa) and an intracellular portion (567 aa), containing a kinase homology (KH) domain, the dimerization domain and the C-terminal catalytic GC domain [1,7] In the absence of ligand, ... on binding of the ligands atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) to its extracellular domain The NP ⁄ GC-A ⁄ cGMP system has important endocrine functions in the ... protein was eluted in Tris buffer containing 600 lgÆmL)1 2450 synthetic triple FLAG peptide (Sigma-Aldrich) The protein concentration was determined by the bicinchoninic acid protein assay (Interchim,...
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Báo cáo khoa học: In vivo degradation of nitric oxide synthase (NOS) and heat shock protein 90 (HSP90) by calpain is modulated by the formation of a NOS–HSP90 heterocomplex pot
Ngày tải lên :
30/03/2014, 04:20
... al In vivo degradation of NOS and HSP90 by calpain higher than in aorta, resulting in a much higher HSP90 to NOS ratio in brain (Fig 1C) A Calpain activation in rat brain and aorta following ... of inducible nitric oxide synthase (iNOS) by calpain I Biochim Biophys Acta 1568, 216– 224 23 Su Y & Block ER (2000) Role of calpain in hypoxic inhibition of nitric oxide synthase activity in ... immunoblotting Desmin and talin were detected with specific mAbs involved in such complexes by submitting samples of crude extracts of rat brain and aorta to gel filtration chromatography As shown in Fig...
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