... 5Â-GGAGCAGCTGGAGTCATG-3Â and 5Â-GGTCATCCTGTATCCTTCA-3Â (the 5Âend of these primers corresponds to the positions 16 3 and 270, respectively, of [3 510 47 45] from the Rattus norvegicusWGS trace database). ... samemethod using the following first primer(CTTGTCTTCACTCCTTGGCTGGCTCCCAT) and the adaptor AP1 primer, followed by a second nestedPCR with the second nested primer (CATCTGGGAGGGGACCTTCAACATCGAC). ... codistributed at the cellular level. It is unlikely that mAb ED3, the anti-B thatwe used, detected the related pseudo-B or Gala1,3Galepitope as the antibody did not react with the syntheticdisaccharide...
... motivation and reten-tion of these mid-level cadres, we must begin measuring and monitoring the key factors within their work environ-ment that affect their performance. The role of organizational ... shortage [12 ].Several studies have shown the link between these organ-izational attributes and job satisfaction [13 -15 ], burnout [16 ], retention and recruitment [12 ,17 ], decreased mortal-ity and ... plans to seek other employment and plans to leave their jobs within the next 12 months.These findings not only confirm the relationship betweenorganizational attributes and job satisfaction and...
... traffic and collisions. In other words, the older pilots took longer to catch on to the new test at first,but they outperformed younger pilots when it came to doing what was most important—keeping ... after gathering more than forty years of data, it was clear that as the women moved into middle age, their moods got better, not worse. At the same time, according toSoto, they also “became more ... at them now . . . I was struck by the fact that even though I am devoted to yoga and eat and get loads of rest and take vitamins and do all the other things you’re supposed to do to maintain the lustrous...
... proofreading the manuscript.ix 12 introductionEven more useful to us, the introductory letters often connect the worksintroduced to other, previous works by Archimedes. Thus the author of the Archimedean ... Department at StanfordUniversity. I am grateful to all these institutions for their faith in the importance of this long-term project.Perhaps the greatest pleasure in working on this book was the ... ascribed to Archimedes because they start with a letterby Archimedes, introducing the work by placing it in context: assuming theseare not forgeries (and their sober style suggests authenticity), they...
... accumulation of the OXI1 transcript inresponse to oxidative stress was stronger than that of the PTI1 -4 transcript.If OXI1 and PTI1 -4 function together in Arabidop-sis, the expression pattern of the ... (A) Yeasttwo-hybrid assays with OXI1 fused to the GAL4 DNA-bindingdomain orthe empty vector pBD, with PTI1 -1 or PTI1 -4 fused to the activation domain orthe empty vector pAD. (B) Yeast two-hybrid ... required for the interaction with PTI1 -4. OXI1 interacts with PTI1 -4 in vivoBecause various PTIs interact with AGCs VIII in vi-tro, the interaction between OXI1 and PTI1 -4 proteinswas tested in...
... binding to nDer p 1 was twice as potent than to the recombinant protein (2.2 mean ratio; 95% conđdenceinterval 2.0 to 2 .4) . Endo H treatment did not alter the results signiđcantly (n 14 ; not shown), ... yeast-derived proteases facilitated the maturationprocess.Both the propeptide a nd the m ature sequence o f Der p 1 contain a putative N-glycosylation site, although Jacquetet al. h ave reported ... der Lecq and others for t heir quick and excellent work on the protein s equences (Sequentie centrum, Utrecht, the Netherlands), and Dr Maurits de Planque for his explanations,time, and help,...
... 5Â-GCGAGGACCAAGGGGATTCTGGAGCTGAACAAGGTGCAATTGTTGTACGAACAGGTGTGCCAGTCCTCC/5Â-GGAGGACTGGCACACCTGTTCGTACAATAATTGCACCTTGTTCAGCTCCAGAATCCCCTTTGGCCTCGC and 5 ¢-CTTGCTAGAACCAAAGGATTTCTGGGGTTGAACAAAATAAAAGGGCTGGCTCGGCAAATGGGATCAAACGCAGAC/5¢-GTCTGCGTTTGATCCCATTTGTCGAGC CAGCCCTTTTATTTTGTTCAACCCCAGAAATCCTTTGGTTCTAGCAAG. The mammalian ... ACTCAAGGGATTGTAGCCTTCCGGCAGCATCTACAGAATCTCGCGAGGACCAAGGGGTTCCTG/5Â-CAGGAACCCCTTGGTCCTCGCGAGATTCTGTAGATGCTGCCGGAAGGCTACAATCCCTTGAGTGAGAGACGTATC and 5Â-GATACGTCCCTTACACAAGGACTTAAGGCATTTAGACAACAGCTTCGGAAGAATGCTAGAACCAAAGGATTTCTG/5Â- CAGAAATCCTTTGGTTCTAGCATTCTTCCGAAGCTGTTGTCTAAATGCCTTAAGTCCTTGTGTAAGGGACGTATC, respectively. The ... SIK1 and SIK2 were mutated with 5Â-GATACGTCTCTC ACTCAAGGGATTGTAGCCTTCCGGCAGCATCTACAGAATCTCGCGAGGACCAAGGGGTTCCTG/5Â-CAGGAACCCCTTGGTCCTCGCGAGATTCTGTAGATGCTGCCGGAAGGCTACAATCCCTTGAGTGAGAGACGTATC...
... available. The reason for this is unclear—what accounts for the morbidity and resultant mortality? In an attempt to understand and identify the leading causes and factors implicated in this morbidity ... undertaken in the hope that it will afford the patient pain relief andthe possibility, or opportunity, to return to pre-injury mobilisation levels. Despite the best efforts of the surgical and ... on the care of patients with fragility fractures. It illustrates the need to look upon the original admission as a opportunity to identify at risk patients and institute measures to optimise...
... becoming the targets of widespread antiretroviral therapy. As the epi-demic and treatment efforts mature, there is the expecta-tion that differences in resistance patterns may emergebetween divergent ... HIV -1- infected patients. Antivir Ther 2002, 7 :12 3 -12 9. Abstract 13 . Meynard JL, Vray M, Morand-Joubert L, et al.: Phenotypic or geno-typic resistance testing for choosing antiretroviral therapyafter treatment ... enzymes, the reversetranscriptase (RT) andthe protease. Antiretroviral drugresistance is common as treatment efforts intensify, and isboth a cause and a result of virologic treatment failure and incomplete...
... IndiaS.S. HasanEditorIndian Institute of Astrophysics, Bangalore, IndiaR.J. RuttenEditorSterrekundig Instituut, Utrecht University, Utrecht, The NetherlandsInstitutt for Teoretisk Astrofysikk, ... with the setting of the second slitat the correct wavelength. So, although spectroheliograms were obtained in 19 05 and 19 06, the instrument did not perform to its full potential. It was left to ... calculate the actual pressure from spectral diag-nostics and it turned out that the pressure in the solar atmosphere was only about atenth of that in the Earth’s atmosphere. The solution to the...
... from the details to "see the forest for the trees." But, unfortunately, for most of us when we step back we just see "lots of trees." We pick our favorite one or two and ... there" kept them from seeing the contradictions in their 17 . září 20 04 11 8 ze 41 2 than taking a bus. This quickly attracted so many new customers that, by the third quarter of 19 82, ... this at first, and kept returning to the airline. Thus, there was no apparent penalty for poor service. But during 19 84 and 19 85, 17 . září 20 04 12 2 ze 41 2 13 5 own policies and strategies....