rabbits as an animal model for stress fractures
Báo cáo y học: " Response of a simian immunodeficiency virus (SIVmac251) to raltegravir: a basis for a new treatment for simian AIDS and an animal model for studying lentiviral persistence during antiretroviral therapy" pptx
... Welfare Act and The Guide for the Care and Use of Page 14 of 19 Laboratory Animals, as well as according to animal care standards deemed acceptable by the Association for the Assessment and Accreditation ... pellet, and the supernatant was poured off The cell pellet was lysed with ml of DNASTAT for 10 250 μl of chloroform was added and the mixture was vortexed The samples were spun at 13,000 for 60 and ... treatment for simian AIDS and an animal model for studying lentiviral persistence during antiretroviral therapy Retrovirology 2010 7:21 Submit your next manuscript to BioMed Central and take full advantage...
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Báo cáo y học: "Hepatic oxidative stress in an animal model of sleep apnoea: effects of different duration of exposure" potx
... the animal studies DPR and JGS collected tissues and performed analyses DPR and DM wrote the manuscript JNP, NPM and DM reviewed the manuscript DPR and DM designed the study and reviewed the manuscript ... enzymes aspartate aminotransferase (AST), alanine amino transferase (ALT), and alkaline phosphatase (ALP), parameters of liver damage, showed no significant difference between the IH-21 group and ... heparinised glass capillary [26] to complete the hepatic integrity (AST, ALT and ALP) test and comet assay We removed the liver of animals for histological analysis; the rest were frozen -80°C for later...
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Báo cáo sinh học: "Univariate and multivariate parameter estimates for milk production traits using an animal model. I. Description and results of REML analyses" pptx
... matrices and more than random effects in the linear model For the analyses described above, only random effects (animal and residual) were fitted, so that a &dquo;standard&dquo; canonical transformation ... selection (if any) has acted on these data; 2) analyses using a repeatability model for each of M, F and P in lactations and For this model it was assumed that the genetic correlation of performance between ... (co)variances estimated for lactation and will be biased if culling was based on performance in the first lactation For all BV analyses the fixed effect structure was different for both traits For...
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Báo cáo sinh học: "Univariate and multivariate parameter estimates for milk production traits using an animal model. II. Efficiency of selection when using simplified covariance structures" docx
... choice for the transformation matrix h V is a canonical transformation on M F and P This transformation was l , , 1 calculated (see Hayes and Hill, 1980 ; and lMeycr, 1985, for computations) and ... scaling factor for genetic variances for the transformed scale (transformation such that new variates are uncorrelated) in lactation was 1.74, and the estimate of the average scaling factor for environmental ... Univariate and multivariate parameter estimates for milk production traits using an animal model I: Description and results of REML analyses Genet Sel Evol, 24, 415-430 Wiggans GR, Misztal I, Van Vleck...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 1
... dedication and passion for research I would like to thank the students and staff of BIOMAT Centre, past and present, for their help and the pleasure of their company: Chee Kong, Fenghao, Kay Siang, ... Solution to Organ Shortage Organ transplantation has become increasingly common in the past 30 years and has saved many lives, while having improved the quality of lives of thousands of other ... Choolani and S-C Wang (2009) "Microgel Iron Oxide Nanoparticles For Tracking Human Fetal Mesenchymal Stem Cells Through Magnetic Resonance Imaging." Stem Cells (In press) Tan K, Tang K, Huang...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 2
... same medium, fibroblastic cells quickly grew out from fetal liver mononuclear cells, and failed to express endothelial phenotype 122 Chapter Isolation and Characterisation Of Vascular Progenitors ... from cord blood (a, b) In contrast, outgrowth colonies from fetal blood (c) were heterogeneous in nature, adopting morphologies ranging from cobblestone, fibroblastic to spindle (d - f) 123 ... Characterisation Of Vascular Progenitors (a) (b) (c) (d) (e) (f) Figure 4-4: CFU assay Adherent colonies appeared after ten days of culture, and were stained with crystal violet to facilitate visualization Cobblestone...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 3
... cultures for haematopoietic colony forming assays as an assessment of the retention of haematopoietic character following expansion As seen in Figure 4-10, Colony forming assays of expanded FL133+ ... demonstrate an ability to take up acLDL at passage three (92.3%) 4.3.2.2.2 Increased vasculogenic potential of FB EPC Matrigel tube formation assays are commonly used as an in vitro assay of endothelial ... on endosomes, as well as on the plasma membranes (Ewan 2006) Finally, vWF is found to be highly localised in granular ultrastructures corresponding to Weibel-Palade bodies In the case of FB-EPC,...
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Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 4
... contacting surfaces has been studied in vascular tissue engineering, which can be broadly classified under three categories: adhesion proteins, anticoagulants and antibodies I had chosen a candidate from ... selection of CD34 antibodies as a suitable conjugand, and I detail here my studies on cell adhesion, as well as blood compatibility Finally, I studied the assembly of cells and film to form a layered ... haemangioblastic cells, demonstrating capacity for haematopoietic, as well as angioblastic differentiation Gehling et al previously demonstrated the isolation of similar putative haemangioblasts...
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An asthma allergen specific animal model for the study of responses to dust mite allergen induced asthma
... results of animal studies to clinical applications as asthma in humans appear to be a far more heterogeneous disease than in animal models The use of surrogate allergen like OVA in animal studies ... Basophils, eosinophils and mast cells release immune mediators that can modulate the immune response Eosinophils can also degranulate to release an array of cytotoxic granule proteins that can ... contribute to AHR and airway remodeling Elevated levels of enzymes released by neutrophils such as matrix metalloproteinase-9 (MMP-9) and elastase were found in BAL and sputum of asthmatic patients...
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Pharmacological interventions in heat stress based on an animal model
... protease of caspase 3, were obtained from Santa Cruz Biotechnology and were used as the primary antibody Bovine anti-goat IgG-HRP from Santa Cruz Biotechnology was used as the secondary antibody ... Further, costs and/or handling of such animals not encourage their use in routine studies Rats and rabbits are the most convenient and readily available standard laboratory animal species and they ... PHARMACOLOGICAL INTERVENTIONS IN HEAT STRESS BASED ON AN ANIMAL MODEL SHANKAR BABU SACHIDHANANDAM B.Sc Pharm (Hons, NUS) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE DEPARTMENT OF PHARMACOLOGY...
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The phenomenon of evaporative cooling from a humid surface as an alternative method for air-conditioning
... comfort area defined by ASHRAE (Figure 30) As well as the other two cases, cooling is performed as a consequence of an increase in the humidity level, though in this case it is more effective for ... economic solution than installing an only system In 1986, Anderson tested an air conditioning system using a direct and an indirect evaporative cooler combined with an expansion battery, and compared ... advantages and disadvantages and indicating and establishing some basis about their design, was developed by Dr John R Watt, who worked for the Research Laboratory of the U.S Navy He built and...
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Tài liệu Báo cáo khoa học: "An Unsupervised Model for Joint Phrase Alignment and Extraction" ppt
... contains itself as its base measure, and stochastic process models that include themselves as base measures are deficient, as noted in Cohen et al (2010) Practically, while the Pitman-Yor process ... distributions, and thus the parameters for the Pitman-Yor process will be different for each distribution Further, as ll and lr must be smaller than l, Pt,l no longer contains itself as a base measure, and ... Language Translation Percy Liang, Ben Taskar, and Dan Klein 2006 Alignment by agreement In Proceedings of the Human Language Technology Conference - North American Chapter of the Association for...
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Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation pptx
... Glaubitz and Andreas Engel for valuable discussions, and we thank Walter Rosenthal for the cDNA of human ETB We further ´ thank Robert Tampe and Katrin Schulze for their help with SPR analysis ... Switzerland), and 0.1% (w ⁄ v) Triton X-100 Horseradish peroxidase-conjugated T7-tag antibody (Merck Biosciences) was diluted : 5000 and incubated for h with the membrane Washed blots were analyzed ... Homo-oligomerization of rhodopsin-like GPCRs is an increasingly recognized mechanism, and might represent an important platform for the modulation of GPCR activities such as ligand binding, signaling or trafficking...
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Báo cáo Y học: An alternative model for photosystem II/light harvesting complex II in grana membranes based on cryo-electron microscopy studies pptx
... software and Dr S Prince, Dr S V Rue and Prof G Garab for useful suggestions and debate T D Flint is thanked for plant growth and specimen preparation as well as L Child and P McPhie for expert ... alternative model, if correct, has several implications for understanding PSII function ranging from light Fig Models for grana ultrastucture (a) Existing, widely accepted model of thylakoid ultrastructure ... Tris/urea-treated membranes as determined by SDS/PAGE and Coomassie staining The left lane shows band a and the right lane band c Molecular mass markers are indicated on the left of the panel Tris/urea-treated...
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Báo cáo khoa học: "An Unsupervised Model for Statistically Determining Coordinate Phrase Attachment" pptx
... development the model will be tested on a testing corpus When evaluating the effectiveness of an unsupervised model, it is helpful to compare its performance to that of an analogous supervised model The ... increase the usefulness of the training data Acknowledgements We thank Mitch Marcus and Dennis Erlick for making this research possible, Mike Col]in.~ for his guidance, and Adwait Ratnaparkhi and ... subsidiaries_NNS and_CC consulting_VBG armsANNS specializing_VBG in_IN tradeANN ,_, lobbying_NN and_CC other_JJ areas_NNS _ From there, it is passed to the chunker yielding: IBecause this tagger trained on annotated...
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nanocarriers as an emerging platform for cancer therapy
... reduced toxicity and enhanced anti-metastatic effects in two different mouse tumour models, emphasizing the advantages of a mechanism-based design for targeted nanocarriers84 Organic nanoparticles ... selectivity can be increased through ligand dimerization or by screening for conformational-sensitive targeting agents such as affibodies, avimers and nanobodies, as well as intact antibodies and their fragments ... Box Rational design of nanocarriers for cancer therapy Nanocarriers can offer many advantages over free drugs They: For rapid and effective clinical translation, the nanocarrier should: • • •...
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Servant-leadership An effective model for Project Management
... opportunity and the foundation for continuing education, many thanks To my daughters Michelle and JoAnne, thanks for your understanding and I hope this work will provide encouragement, for you both, ... project management has experienced a shift toward a stronger emphasis and focus on the organizational and human aspects of project work This is in comparison to the past, where the emphasis was more ... change amidst constantly changing world conditions His model of transformational leadership refers to a transformation in the assumptions and thoughts of followers while creating a commitment for...
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Báo cáo hóa học: "Generalized cerebral atrophy seen on MRI in a naturally exposed animal model for creutzfeldtjakob disease" pot
... in various stages of disease For this reason, these animals are considered a good model to study MRI findings in scrapie as a model for the TSE diseases Our objective was to study the consistent ... [12,13] Each animal was scanned live under general anaesthesia and then recovered with the exception of the three most clinically affected animals, which were euthanized after the MRI exam, and one ... sheep The quantification of certain brain parameters on MR images, such as the V/C ratio as used in this study, may be considered as an ante-mortem tool for live animals at risk for scrapie,...
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Báo cáo sinh học: " Recombinant HPV16 E7 assembled into particles induces an immune response and specific tumour protection administered without adjuvant in an animal model Linda Petrone1, Maria G Ammendolia2, Armando" ppt
... article as: Petrone et al.: Recombinant HPV16 E7 assembled into particles induces an immune response and specific tumour protection administered without adjuvant in an animal model Journal of Translational ... 66Zn and 68Zn analytical masses The GST protein was produced in pGEX-2T transformed E coli and purified by glutathione affinity chromatography (PIERCE) Measurements were performed by means of ... discussing this manuscript, Mr Andrea Giacomelli and Mrs Monica Gabrielli of the MIPI -animal care unit, and Mr Valter Tranquilli for assistance in computer artwork The work was supported by the Project...
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báo cáo hóa học: "Infrared thermography as an access pathway for individuals with severe motor impairments" docx
... placement This participant had an awkward position in his wheelchair (See Figure 3(b)) which forced us to position the thermal camera at an angle and distance from the participant that was not consistent ... Kiguchi M, Kanazawa T: A communication means for totally locked-in ALS patients based on changes in cerebral blood volume measured with near-infrared light IEICE Transactions on Information and Systems, ... face region If one can voluntary control mouth open and close action, sip and puff technology, EMG based switches, and computer vision based switches can also be used The advantage of the proposed...
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