... were digested with EcoRI and SalI, and inserted into the p-GBK vector (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the SalI and BamHI sites in the vector The DNA sequences of all fusion ... construction To fuse emerin, Btf or fragments thereof with the twohybrid vectors, the desired cDNAs were PCR-amplified using the primers and templates in Tables and PCR products were digested with NdeI and ... specificity of the antibody Total HeLa cell extracts (total) and corresponding cytoplasmic (cytosol) and nuclear (nucleus) fractions were resolved by SDS/ PAGE, and immunoblotted with either affinity-purified...
... hành động học sinh theo chiến lược hợp lý cho học sinh tự chủ chiếm lĩnh tri thức cho theo chiến lược hợp lý từ để học sinh chủ động chiếm lĩnh tri thức cho lực trí tuệ nhân cách to n diện họ bước ... thực tổ chức hoạt động dạy học theo quy trình hợp lý.Khi thiết kế tiến trình hoạt động dạy học theo hướng xây dựng tình học tập định hướng hoạt động nhận thức học sinh theo tiến trình xây dựng tri ... khoa học, thức tiến trình dạy học theo pha theo tiến trình nghiên cứu tình học tập hướng dẫn học sinh giải tình học tập Tiến trình dạy học gồm ba pha (hình 1.3b), theo thể hình 1.3 25 2.1.2 Tiến...
... chromosome from their mothers and a Y chromosome from their fathers The X-Y combination makes them male Girls inherit two X chromosomes, one from their mothers and one from their fathers The X-X combination ... of musculardystrophyThe other major types of musculardystrophy include: • • • • • Limb-girdle musculardystrophyCongenitalmusculardystrophy Oculopharyngeal musculardystrophy Distal muscular ... Distal musculardystrophy This group involves the muscles farthest away fromthe center of the body — those of the hands, forearms, feet and lower legs The severity is generally less than for other...
... structure of the fibril andthe b-strand arrangements remains to be determined Yet, knowledge of the structure and biophysical analysis of the fibrils alone will not suffice for understanding the disease ... propensity of the N-(+7)Ala variant to adopt the b-cross state Fibrils of N-WT and N-(+7)Ala also differ with respect tothe fibril growth rates In the case of N-WT, the conversion tothe fibrillar ... and N-(+7)Ala were also observed at the level of fibril morphology A likely interpretation is that the number of alanines determines the arrangement of the b-strands leading to variations in the...
... shown) and protein disulfide isomerase (PDI) (Fig 6), suggesting they were located in the cytoplasm and therefore excluded fromthe ER and thus unable to be transported tothe nuclear envelope These ... The tryptic peptides fromto 176 emerin were extracted fromthe gel slices, micropurified by Fe3+ IMAC and introduced into the MALDITOF mass spectrometer All the protein bands extracted had a ... pBluescript KS- to synthesis the emerin S49A and S49E mutants [9] The restriction enzyme sites HindIII and BamHI were incorporated at the 5¢- and 3¢-ends, respectively The PCR fragments generated were...
... DAD), as well as support fromtheMuscularDystrophy Association (to DCG and JMP) andthe American Heart Association (EIA 0740040N to PMLJ) The authors declare that they have no competing interests ... contributions DAD performed the histology, bred and genotyped the dko mice YX performed the muscle experiments and analyzed the data JP designed the treatment regimen, treated the mice, and performed EMSA ... cut fromthe tissue blocks and used for the following staining procedures Page of 10 For viewing of gross histology, sections were fixed in 100% ethanol and then stained with hematoxylin and...
... DAD), as well as support fromtheMuscularDystrophy Association (to DCG and JMP) andthe American Heart Association (EIA 0740040N to PMLJ) The authors declare that they have no competing interests ... contributions DAD performed the histology, bred and genotyped the dko mice YX performed the muscle experiments and analyzed the data JP designed the treatment regimen, treated the mice, and performed EMSA ... cut fromthe tissue blocks and used for the following staining procedures Page of 10 For viewing of gross histology, sections were fixed in 100% ethanol and then stained with hematoxylin and...
... BVN is the first author and is responsible for the conception and design of the case report Contributing authors SD, AS, SS, JK and DKM made substantial contributions tothe design of the manuscript ... myofibers (0.5% to 0.9%) The discovery of the donor’s mesenchymal cells in the patient’s muscle tissue and bone marrow 13 years after the transplant raised the hope that BMT may play a role in the treatment ... improved motor activity and in laboratory parameters seen by reduction in CPK levels, increase in dystrophin-positive muscle fibers and Conclusion Tothe best of our knowledge, we report the first...
... responsible for the format and revisions of the manuscript IA and MP were the treating anaesthesiologists while TP and AF were the interventional radiologists who performed the embolization TP and AF ... involved in the diagnostic work-up and management of the patient IZ also performed the retrograde study and wrote the case presentation section KP is the head of the Department of Urology and was ... a ureteral catheter into the right pelvis was carried out in order to selectively obtain a sample for urine cytology and perform a retrograde study Cystoscopy and upper tract cytology were both...
... treatments to alter the course of the disease and improve the quality of life for patients Previous studies have shown that two thirds of patients receive the mutation from their mothers andthe other ... of the genetic X- linked inheritance) According to Mendelian inheritance, there is 50% chance a mother who carries the DMD gene can pass the X chromosome carrying DMD mutated genetothe sons and ... include: gene therapy, read-through stop codon strategies, stem cell therapy, vival vectors and utrophin [59] Nonetheless, these methods are only of partial support for patients and they are not the...
... mother who carries the DMD gene can pass the X chromosome carrying DMD mutated genetothe sons and they will develop disease and 50% chance that her daughters will carry thegene A carrier mother ... treatments to alter the course of the disease and improve the quality of life for patients Previous studies have shown that two thirds of patients receive the mutation from their mothers andthe other ... rapid, sensitive and accurate The long term objective of this research is to apply MLPA to detect DMD mutated gene in carriers andfrom which, we aim to further develop the project into early diagnosis...
... tothe toiling and dying types who made it over to America And once in America, people divided once again: you could say they became the poor andthe rich The losers and winners The artists and ... traces the progression, in ‘‘[moving] fromthe organic growth of the plant (the great British oak) tothe countryside, the country house andthe georgic ideal of retirement, the estate, the aristocratic ... kind of stories that get told and retold about the Irish, so as to reveal both the regularity of English colonial discourse on Ireland andthe Irish andthe mutations to which that discourse is...
... tổ chức máy kế to n xí nghiệp giầy thể thao xuất kiêu kỵ - gia lâm Kế to n trưởng kiêm kế to n tổng hợp - Kế to n to n tiền lương Kế to n nguyên vật liệu, công cụ, dụng cụ - Kế to n giá thành ... hạch to n để hạch to n hàng ngày tình hình nhập - xuất - tồn kho Yêu cầu trình độ quản lý, trình độ kế to n cán kế to n doanh nghiệp tơng đối cao 15 Sơ đồ kế to n chi tiết nguyên liệu, vật liệu theo ... kế to n xí nghiệp Do đặc điểm tổ chức sản xuất nh đặc điểm quản lý xí nghiệp nên máy kế to n xí nghiệp giầy đợc tổ chúc theo hình thức kế to n tập trung, công tác kế to n đợc thực phận kế to n...
... line The customs formalities need to be renewed to facilitate export and import The General Department of Customs, the Ministry of Trade and examining bodies, andthe Bureau of Standards and Measures ... in the Mekong Delta linking to Cambodia The total length of the inland waterway (IW) system is about 40,998 km, mainly in the Red River Delta andthe Mekong Delta In the past few years, inland ... above are the keys for Vietnam freight forwarding industry to open the door of development now and in the future How they use those keys properly to open the door? It depends on other factors, which...
... from customer’s information in the past and then automatically classify the customers and group them properly Then the results will be directly sent back tothe customers It is enclosed by a bank’s ... meeting the demand and needs of the market economy with the unceasing diversification and development in the demand to use finance-banking products, services of the customers, aiming to prepare ... pay the debts, the bank can sell the assets by order of the court immediately It is not essential to take each other tothe court It is costly and possible to create loss The Government need to...
... cáo kế to n Kế to n trởng ngời tham mu cho Giám đốc việc áp dụng chế độ quản lý nhà nớc ban hành cho phù hợp với tình hình sản xuất xí nghiệp * Kế to n to n kế to n lơng:Theo dõi vấn đề to n, ... tổ chức máy kế to n xí nghiệp giầy thể thao xuất kiêu kỵ - gia lâm 38 Kế to n trưởng kiêm kế to n tổng hợp - Kế to n to n tiền lương Kế to n nguyên vật liệu, công cụ, dụng cụ - Kế to n giá thành ... thuế GTGT theo phơng pháp trực tiếp (*) Cơ sở kinh doanh nộp thuế GTGT theo phơng pháp Hệ thống sổ khấu trừ sử dụng kế to n tổng hợp vật liệu: Mẫu sổ tuân kế to n thủ theo chế độ kế to n áp dụng...
... người Ở đây, tác giả muốn đề cập đến người phát triển to n diện mặt đức - trí – thể - mỹ Nếu thiếu mặt “con người to n diện” Bác Hồ lời kêu gọi to n dân tập thể dục, người cho thấy rằng: “Giữ gìn ... thời giúp cho người tập luyện TDTT thường xuyên có thể hình (1) p, đẹ cân đối Chris Gratton - Sport, Health and Economic Benefit– Sport Industry Research Centre, Sheffield Hallm University, 2004 ... vậy, TDTT có vai trò to lớn người xã hội Trong thập niên gần đây, nước công nghiệp phát triển, người dân có nhận thức rõ ràng vai trò việc rèn luyện thân thể chất lượng sống Theo số thống kê tài...