pyruvate lactate acetate glycerol and ethanol have all been measured to have increased gene expression in grr1δ cells grown on high glucose concentrations 2 159 the chang

Báo cáo khoa học: Efﬁcient inhibition of b-secretase gene expression in HEK293 cells by tRNAVal-driven and CTE-helicase associated hammerhead ribozymes doc

Ngày tải lên : 08/03/2014, 08:20

... protein level in HEK293 cells The test cells were transfected with Rz-1 and Rz -2 encoding plasmids and 36 h postincubation the protein fraction was isolated from the cells and subjected to Western ... Rz-1 and Rz -2, in comparison to the level of a control experiment with lipofectin only transfected cells A further decrease of the Ab level up to 20 % was observed in the following 12 h However, the ... mRNA in Rz-1 transfected HEK293 cells was also monitored in the course of time under single gene ampliﬁcation RT/PCR conditions (Fig 4) Cells were lysed after 6, 12, 24 , 36 and 48 h of the postincubation...

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Báo cáo y học: "Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte" pptx

Ngày tải lên : 09/08/2014, 06:22

... Disruption of the actin cytoskeleton results in nuclear factorkappaB activation and inflammatory mediator production in cultured human intestinal epithelial cells J Cell Physiol 20 04, 20 0:71-81 ... rounded/cuboidal to more flattened and spread cells [29 ] These morphological changes are accompanied by changes in the organization of the actin cytoskeleton [30] Coincident with the change in chondrocyte ... regulating the aggrecan and type II collagen genes and in remodeling of the cytoskeleton in response to factors present during inflammation The MAPK/ERK pathway plays an important role in directing...

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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"

Ngày tải lên : 02/11/2012, 11:08

... IL5: the interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: ... upregulated genes there were the a-myb oncogene, the interleukin receptor gene (IL4R), the gene of the transporter protein TAP2, the gene of protease calpain small subunit (capn4) and other (in case ... Cytokine gene transcription in SIV and HIV-associated non-Hodgkin's lymphomas AIDS Res Hum Retrovir 1997; 13: 1589 -1596 Int J Med Sci 20 05 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 ...

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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt

Ngày tải lên : 18/02/2014, 18:20

... glia cells found in the PNS In addition to their roles in myelination, trophic support and axon regeneration, SCs exhibit potential immune functions, similar to the non-myelinating glia of the ... upregulation [5,7 ,22 ,23 ], mediates the rapid induction of cytokines and adhesion molecules that are implicated in immune and in ammatory responses [24 ,25 ] Mitogen-activated protein kinases (MAPKs) ... ICAM-1–integrin interactions mediated adhesion of leukocytes to the vascular endothelium, revealing a key role in migration of leukocytes to in ammation sites [28 –30] In the control rats, most of the...

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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

Ngày tải lên : 19/02/2014, 16:20

... (1996) Inhibitory effect of fish oil n-3 polyunsaturated fatty acids on the expression of endothelial Ó FEBS 20 04 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Anti-mitogenic action of EPA and DHA ... mitogen and effective co-mitogen acting in conjunction with other growth factors [2] The present study confirms that the incorporation of AA increases the proliferation of SMC [3,4], but only in ... IL-1b-induced binding of these factors In addition, the expression of genes, associated with atherosclerosis, that contain overlapping GC-box expression are controlled at the transcriptional level...

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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt

Ngày tải lên : 06/03/2014, 19:20

... mitochondria in eWAT (Figures 2A and 2B) and iBAT (Figures 2C and 2D), respectively, and the analyses of mitochondria number (Figure 2E) and area (Figure 2F) In the PM2.5-exposed group, the mitochondrial ... 39.5 23 .1 Co Br 10.0 6.5 7.3 3.3 35 .2 33.9 21 .9 12. 7 Se 8.1 5.6 28 .2 15.0 I 5.7 15.7 28 .2 55.6 V 8.5 5.0 27 .2 12. 9 Mn 4.1 6.0 24 .9 18.8 Ti 1.8 2. 4 22 .2 20.0 Pb 4.4 6.4 21 .5 21 .8 As Rb 4.3 4.5 5 .2 ... decreased in all defined adipose depots in response to PM2.5 exposure The levels of down-regulation of both these genes were pronounced in iBAT and mBAT in comparison with the WAT depots The gene expression...

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Báo cáo khoa học: Diverging regulation of pyruvate dehydrogenase kinase isoform gene expression in cultured human muscle cells pot

Ngày tải lên : 07/03/2014, 17:20

... by insulin through directly altering the expression of the human PDK2 and PDK4 isoforms In addition to our ﬁndings, insulin has been shown to decrease the mRNA for PDK2 and PDK4 in 7800C1 hepatoma ... by insulin were used Two distinct phosphatidylinositol 3-kinase (PtdIns3K) inhibitors, wortmannin and 2- (4-morpholinyl)-8-phenyl-4H-1-benzopyan-4-one (LY2940 02) [25 ,26 ], were used to examine the ... linked to changes in expression of metabolic genes, by their in uence as transcriptional activators FEBS Journal 27 2 (20 05) 3004–3014 ª 20 05 FEBS Regulation of hPDK2 and hPDK4 gene expression...

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Báo cáo Y học: The proximal cis-acting elements Sp1, Sp3 and E2F regulate mouse mer gene transcription in Sertoli cells potx

Ngày tải lên : 08/03/2014, 23:20

... Axl/Rse expression may be due to the increasing number of germ cells or to the onset of the in uence of hormones involved in spermatogenesis The possible reasons for modulating the expression of these ... interaction of E2F with Sp1 to suppress the transcription of Mer in Sertoli cells Other studies have shown the involvement of TATAless genes containing E2F and Sp1 binding sites in DNA replication and ... Sertoli cells were transfected with the wild-type pGL3/() 321 /+ 126 ) reporter gene constructs or the constructs containing double point mutations at the Sp1 and E2F cis-acting motifs as shown in the...

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Báo cáo khoa học: The intrinsic structure of glucose transporter isoforms Glut1 and Glut3 regulates their differential distribution to detergent-resistant membrane domains in nonpolarized mammalian cells potx

Ngày tải lên : 23/03/2014, 09:20

... procedures These cDNA constructs were then ligated into the expression vector pCMV and used for transient expression by lipofection in CHO cells Initially, we examined whether FG1 and FG3 are able to ... for the DRM distribution, additional FLAG-tagged chimeric constructs, A and B, were generated (Fig 5A) In A, amino acids 2 12 and 27 2–4 92 of Glut1, which include the N-terminal cytosolic tail and ... proteins are preferentially distributed to these microdomains, including glycosylphosphatidylinositolanchored protein, the Src-family tyrosine kinases, FEBS Journal 27 4 (20 07) 28 43 28 53 ª 20 07 The...

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Báo cáo khoa học: Differential gene expression in periportal and perivenous mouse hepatocytes potx

Ngày tải lên : 23/03/2014, 10:20

... gluconeogenesis and ketogenesis in periportal 5060 15 16 17 18 19 20 21 22 23 and perivenous rat hepatocytes Heterogeneity of hormone action and of the mitochondrial redox state Biochem J 25 6, ... through the vena cava and then immediately ﬂushed out from the opposite direction To obtain perivenous hepatocytes, the digitonin solution was infused through the portal vein After digitonin treatment, ... according to their preferential perivenous or preferential periportal expression, using as discriminators a Œlog2 expression ratio Œ ‡ (corresponding to a ‡ 2- fold difference in expression) and...

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Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot

Ngày tải lên : 30/03/2014, 13:20

... polyclonal poneratoxin-speciﬁc antibody For the poneratoxin expression, two recombinant baculoviruses with the poneratoxin gene were engineered, one containing a signal peptide and another without ... virus with the poneratoxin gene (Px) and the third virus with the poneratoxin gene preceded Fig Confocal microscopy of the Sf21 cells expressing recombinant poneratoxin Baculovirus-infected cells ... observed 25 min components might also be neurotoxins Recombinant poneratoxin was more toxic than the synthetic one It should be borne in mind that the recombinant poneratoxin has the 21 amino acid...

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báo cáo hóa học:" Attitudes to routine HIV counselling and testing, and knowledge about prevention of mother to child transmission of HIV in eastern Uganda: a cross-sectional survey among antenatal attendees" docx

Ngày tải lên : 20/06/2014, 08:20

... antenatal clinic 308 (79.4) 80 (20 .6) the handling by clinic staff 344 (88.7) 44 (11.3) the total waiting time in clinic 28 6 (73.7)a 1 02 (26 .3)b the clinic facilities the health education talk 322 (83.0) ... [30,31] and Lilongwe, Malawi [ 32] The availability of rapid HIV testing in the clinic and the giving of same-day HIV test results may have contributed to the high participation in the HIV testing ... the drafting on the manuscript TT participated in the conception and design of the study, interpretation and drafting the manuscript All authors read and approved the final manuscript Competing...

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báo cáo hóa học:" Usefulness of five-item and three-item Mental Health Inventories to screen for depressive symptoms in the general population of Japan" ppt

Ngày tải lên : 20/06/2014, 15:20

... (18.63) 72. 32 (19.55) 619 (20 ) 506 (16) 665 (21 ) 617 (20 ) 479 (15) 22 1 (7) 70.17 (18.47) 72. 50 (17.47) 72. 38 (20 .28 ) 74 .22 (18.60) 75 .21 (19.11) 73 .23 (21 .09) 385 ( 12) 670 (22 ) 685 (22 ) 648 (21 ) 22 8 ... 69.95 (20 .88) 622 (20 ) 22 27 ( 72) 28 (1) 65 (2) 1 52 (5) 13 (0) 70.04 (18.89) 73.74 (18.8) 75.43 (16.86) 68.66 (20 .91) 72. 18 (22 .27 ) 70.00 (19.71) 1610 ( 52) 29 9 (10) 164 (5) 171 (6) 533 (17) 22 6 (7) ... the SF-36, the MHI-5 was administered as a paper -and- pencil questionnaire The instrument contains the following questions: 'How much of the time during the last month have you: (i) been a very...

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Báo cáo hóa học: " Effective harvesting, detection, and conversion of IR radiation due to quantum dots with built-in charge" docx

Ngày tải lên : 20/06/2014, 22:20

... simulation tool self-consistently solves Schrödinger, Poisson, and current equations for electrons and holes The conduction and valence bands of the structures are defined within a single-band or ... approximately 42% , in the case of concentrator cells Technological limitations are determined by the need to match crystalline lattices, thermal expansion coefficients, and the most difficult, to match all ... improve the performance of IR photodetectors and solar cells These improvements are due to effective harvesting of IR radiation via intra-band QD transitions and transitions from QDs to the conducting...

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Báo cáo hóa học: " Multidimensional characterization, Landau levels and Density of States in epitaxial graphene grown on SiC substrates" pdf

Ngày tải lên : 21/06/2014, 05:20

... ratio I2D/IG between the integrated intensities of the 2D-band and the G-band is high, which suggests weak residual doping in the order of to × 10 12 cm -2 [18] Altogether, these Raman and microscopy ... temperatures, there is an additional contribution to the conductivity, which is visible in Figure 5b as a change in the slope We attribute this additional contribution to hopping In principle, from the ... fields in the vicinity of the RK /2 plateau The activation energy Ea varies from 0.7 to 3.3 meV between B = 10 and 13 T, which remains much smaller than the distance between the first and the second...

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Báo cáo hóa học: "Alloying and Strain Relaxation in SiGe Islands Grown on Pit-Patterned Si(001) Substrates Probed by Nanotomography" doc

Ngày tải lên : 21/06/2014, 20:20

... Spatially resolved 3D contours of the Ge content, xGe, within the islands were obtained by measuring the same surface areas at increasing etching times [11] The lateral and vertical resolution in ... Conclusions In conclusion, an AFM-based nanotomography approach was used to gather in- depth information about the alloying and relaxation mechanism on both flat and pit-patterned substrates The 3D ... a major role in determining strain relaxation and therefore in defining the compositional profiles of the islands Experimental Procedure The sample considered here consists of 8.5 monolayer of Ge...

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Báo cáo y học: "All-trans retinoic acid suppresses interleukin-6 expression in interleukin-1-stimulated synovial fibroblasts by inhibition of ERK1/2 pathway independently of RAR activation" ppsx

Ngày tải lên : 09/08/2014, 01:22

... 16 17 18 19 20 21 22 23 24 25 26 27 28 Tan PL, Farmiloe S, Yeoman S, Watson JD: Expression of the interleukin gene in rheumatoid synovial fibroblasts J Rheumatol 1990, 17:1608-16 12 Miyazawa K, ... thought to contribute to the inhibitory effect of ATRA on IL-1induced expression of cytokines in chondrocytes [34], these data highlight that ATRA may act in a cell-dependent manner In contrast, ... transcription factors in the control of many inflammatory mediators, liganded RAR complexes can repress a broad spectrum of genes, including inflammatory proteins, cytokines, or matrix metalloproteases...

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Báo cáo y học: "Histone deacetylase inhibitors modulate metalloproteinase gene expression in chondrocytes and block cartilage resorption" ppt

Ngày tải lên : 09/08/2014, 06:22

... compounds have pleiotropic effects on gene expression Conceptually, the action of HDAC inhibitors leading to an increase in histone acetylation should induce expression of susceptible genes, but in ... vivo [41, 42] and these factors induce the expression of a number of metalloproteinase genes in chondrocyte cell lines [46] The addition of either of two chemically distinct HDAC inhibitors to cartilage ... activity against class I and II HDACs, but not the SIR2 family Addition of these reagents to cells should therefore block histone deacetylation and result in increased acetylation of histones on susceptible...

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Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx

Ngày tải lên : 09/08/2014, 10:21

... Tlr 12 Toll-like receptor 12 3.3 381549 Zfp69 Zinc finger protein 69 3 .2 3.3 23 0779 Serinc2 Serine incorporator 2. 2 16818 Lck Lymphocyte protein tyrosine kinase 2. 9 122 60 C1qb Complement component ... the strains Gene expression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the gene expression profiling has been on identifying genes in the two NOD derived congenic ... After log2 transforming the intensities, replacing missing values and collapsing probes to genes, the gene lists were filtered by minimum standard deviation = 0.7 Finally, the genes in the list...

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