purification and assays of homodimeric and heterodimeric forms of rnase iii from bacterial extremophiles and mesophiles
Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... Eco -RNase HI [34], RNase H of Moloney murine leukemia virus reverse transcriptase (MMLV RNase H) [35] and Bacillus stearothermophilus RNase HIII (Bst -RNase HIII) [36] Eco -RNase HI and Bst -RNase ... the RNase H domain of Tma -RNase HI shows relatively low amino acid sequence identities of 20% to Hsa -RNase H1, 19% to Eco -RNase HI, 18% to SIB1 HBD -RNase HI, SceRNase H1 and Bha -RNase HI, and ... acid sequence of Tma -RNase HI (Tma) is compared with those of Bha -RNase HI (Bha), SIB1 HBD -RNase HI (SIB1HBD), Sce -RNase H1 (Sce), Hsa -RNase H1 (Hsa), Eco -RNase HI (Eco) and HIV-1 RNase H (HIV1)...
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Báo cáo khoa học: Identification of RNase HII from psychrotrophic bacterium, Shewanella sp. SIB1 as a high-activity type RNase H pot
... The two RNases H from each strain differ greatly in metal ion preference and specific activity One of the pair of these RNases H, such as E coli RNase HI, BsuRNase HIII, and Bst -RNase HIII, prefers ... E coli RNase HII, Bsu -RNase HII, and Bst -RNase HII, prefer Mn2+ to Mg2+ for activity The specific activities of E coli RNase HI, Bsu -RNase HIII, and Bst -RNase HIII are higher than E coli RNase ... contain RNases HI and HII, yeast and human cells contain RNases H1 and H2, and Bacillus subtilis and Bacillus stearothermophilus cells contain RNases HII and HIII The physiological significance of...
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Purification and cloning of PCR products
... ordering process and the cost of synthesis is relatively cheap An example of appropriate design of primers to add attB1 and B2 sites to the 5′ and 3′ ends respectively of a gene of interest are ... N-glycosidic bonds between uracil and deoxyribose leading to a loss of the base The loss of bases leads to disruption of base pairing and therefore exposure of single strands that can be annealed to ... ethidium bromide and UV irradiation for detection of DNA in gels, thereby preventing DNA damage of the long molecules and enhancing isolation of full-length clones The range of cloning kits and vectors...
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Tài liệu Báo cáo khoa học: Purification and sequence identification of anserinase ppt
... shown) Cloning of anserinase cDNA PCR was performed using a set of primers (A and B) and the first-strand cDNA for 3¢ rapid amplification of cDNA ends (RACE), prepared from total RNA of 6004 Nile ... molecular identification of anserinase Results and Discussion Purification and characterization of anserinase from Nile tilapia brain The procedure for the purification of the enzyme from Nile tilapia ... including 56 bp of 5¢ UTR, 1482 bp of ORF, and 299 bp of 3¢ UTR The coding region of the sequence was translated into 494 amino acids, which included a typical signal peptide of 18 amino acids and two...
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Báo cáo khoa học: Purification of three aminotransferases from Hydrogenobacter thermophilusTK-6 – novel types of alanine or glycine aminotransferase Enzymes and catalysis pot
... specific activity of AT1 was less than one-fifth of that of AT2, both specific activities were higher than those of reported GGTs (such as 5.71 UÆmg)1 from A thaliana and 3.25 UÆmg)1 from Rhodopseudomonas ... description of the characterization of bacterial GGT and AGT at an enzymatic and gene level Comparison of the amino acid sequences with known enzymes showed the phylogenetic position of 1880 each ... purification, suggesting that the four kinds of activity in CFE were derived from only the three enzymes Purified AT1, AT2 and AT3 gave single bands of 44, 42 and 45 kDa on SDS ⁄ PAGE, respectively...
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Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
... alignment of fragments obtained from MS/MS analyses of FIII-1 and FIII-2 from P excavatus with lumbrokinase and its precursor from L rubellus (sp:P83298 and U25647) and E fetida (gpu:EU167737 and AY438624), ... proteins Generally, the two-step chromatography of AEX and HIC was sufficient for the purification of FIII-1, FIII-2, and FII, since their SEC and SDS-PAGE profiles represented pure proteins Fraction ... subunits of fibrinogen within 10 minutes, and FIII-1 and FIII-2 could even cleave off the g subunit The hydrolytic ability of fraction FIII-2 was the highest since almost no protein bands were...
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Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...
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Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps
... Journal 2010, 7:241 http://www.virologyj.com/content/7/1/241 Page of Figure Expression and purification of the tgK protein M represented standard protein molecular weight markers The arrow marked the ... http://www.virologyj.com/content/7/1/241 Page of Figure Purification of the rabbit anti-tgK polyclonal antibody and Western blot assay M represented standard protein molecular weight markers; M1 ... diluted twofold from 1:1 to 1:64 The 200TCID 50 virus, which was diluted from the virus stock suspension (TCID50 = 10-5.567), in a 25 μl volume was mixed with an equal volume of serum dilution and...
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Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc
... Strains, plasmids, and enzymes Expression and purification of LSV coat protein Figure Expression and purification of LSV coat protein A, Expression and purification of LSV CP 15% SDS-PAGE of cultures ... Alignment of the amino acid sequence of coat protein of different LSV isolates and other carlavirus Figure Alignment of the amino acid sequence of coat protein of different LSV isolates and other ... Laboratory of Arid and Grassland Agroecology of Ministry of Education, School of Life Sciences, Lanzhou University, Lanzhou 730000, China and 4Department of Bacteriology, Graduate School of Medical Science,...
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PURIFICATION AND CATALYTIC REFORMING OF METHANE a NEW INSIGHT INTO CARBON ADSORBENT AND MEIC MEMBRANE REACTOR
... of LBFZ, YSZ and YSZ60-LBFZ40 96 Figure 5.4 TPR profiles of LBFZ, CGO60-LBFZ40 and YSZ60-LBFZ40 98 Figure 5.5 XRD patterns of LBFZ, CGO60-LBFZ40 and YSZ60-LBFZ40 samples before and after TPR ... of LBFZ, YSZ60-LBFZ40 and 99 xii CGO60-LBFZ40 membranes as a function of temperature Figure 5.7 O2-TPD profiles of LBFZ, YSZ60-LBFZ40 and CGO60-LBFZ40 samples 101 Figure 5.8 SEM micrographs of ... properties of porous AC and preparation methods of mesoporous AC, and mixed conductive ceramic membrane including the background of mixed conduction, mechanism of oxygen separation, POM into syngas and...
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Identifying protein co factors of oct4, an essential stemness transcription factor, by affinity purification and mass spectrometry
... Sp1 Affinity Purification/ Tandem MS/MS - - Affinity Purification/ Tandem MS/MS (Wang et al., 2006) - - Affinity Purification/ Tandem MS/MS (Wang et al., 2006) - - Affinity Purification/ Tandem MS/MS ... expression profile and knockout phenotype of Nanog points to a degree of interconnectedness between the roles of Nanog and Oct4 – an Oct4 transgene knockdown results in differentiation of ES cells ... mechanism of DNA methylation in ES cells, the loss of Dnmt3a and Dnmt3b also resulted in a loss of methylation at the key promoters of Pou5f1 and Nanog examined (Li et al., 2007), and a concomitant...
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Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... Structures and molecular weights of soyasapogenol B, C and B1 98 Fig 28 LC-MS Chromatogram of standard and purified sample of soyasapogenol B Panels (A and C) are authentic standard and samples of ... materials and methods 88 Fig 25 The effect of acid and base hydrolysis, environmental temperature and both methanol viii and anhydrous methanol on the generation of soyasaponin I and III ... Analysis of Soyasaponins and Soy isoflavones 79 6.2.1.6 LC/MS Analysis of Total soyasaponins and Soy isoflavones 79 6.2.2 Comparison of Soyasaponin Concentration Methods 80 iii 6.2.2.1...
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purification and characterizations of phytase enzyme form aspergillus niger
... I Introduction II Materials and methods 2.1 Materials 2.2 Methods III Results and Discussion 3.1 Extraction of phytase from A niger fresh biomass 3.2 Purification of phytase 3.2.1 Precipitation ... hexakisphosphate) is the primary storage form of phosphorus in plants They occupy about 1-3% of the seeds of grains and legumes, and 60-80% of the total amount of plant phosphorus (Nelson, 1967) Phosphorus ... Lab of Enzyme Technology, Biotechnology R&D Institute (Nguyen Nhat Khoa Tran, 2012) As the continuation of this research, the thesis on "Purification and characterizations of phytase enzyme from...
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Optimization of expression and purification of HSPA6 protein from Camelus dromedarius in E. Coli
... shown in Fig 7b, the level of cHSPA6 expression reached at its maximal level within 1h of induction and incubation at 37 oC The level of cHSPA6 remained unchanged up to 24h of post-induction incubation ... 12% SDS–PAGE (b) Analysis of purity of eluted protein from Superdex 75 column by silver staining Lane 1, low molecular weight marker; lane 2, Pool of fractions obtained from size exclusion chromatography ... protein was quantified by Bradford method (Bradford, 1976) 1.5 Extraction and purification of cHSPA6 Homogenous preparation of cHSPA6 in two chromatographic steps 1.5.1 Ni–NTA chromatography HisTrap...
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Characteristics of hydrogen production from food waste and waste activated sludge
... was supplemented with 200 mg of KH2PO4, 14 mg of MgCl2•4H2O, mg of Na2MoO4•4H2O, mg of CaCl2•2H2O, 2.5 mg of MnCl2•6H2O, and 10 mg of FeCl2•4H2O, which was modified from Lay et al (1999) NaHCO3 ... produce moles of hydrogen with mole of n-butyrate or moles of hydrogen with mole of acetate from mole of hexose In most cases using soluble defined substrates, hydrogen production yield and major ... M1-0203-00-0063 from the National Research Laboratory Program of the Korean Ministry of Science and Technology REFERENCES APHA, AWWA, WEF, 1998 Standard Methods for the examination of Water and Wastewater,...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... secretory RNases from the zebrash share the RNase A scaffold [7] Small-molecule inhibitors of these RNases could be used to investigate their broad biological functions The afnity of RNase A for ... Ii(h)| Rh RiIi(h), where Ii(h) and I(h) are the ith and the mean measurements of the intensity of reection h, respectively Rcryst = Rh |Fo ) Fc| RhFo, where Fo and Fc are the observed and calculated ... phosphoryl group Results and Discussion Sulfonimides as inhibitors of RNase A We began by determining the ability of three backbone analogs of RNA to inhibit catalysis by RNase A These analogs have...
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Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... removed from the superposition No of corresponding CA atoms RNase RNase RNase RNase RNase Fig Ribbon diagram of RNase Sa2 ˚ with the largest differences (up to 3.6 A) at the N-terminus and in ... Differences in physicochemical properties of RNase Sa2 and RNase Sa No of amino acids 97 96 RNase Sa2 RNase Sa a From [33] b Sequence identity (%) 53 From [34] c From [56] pIa Tm (°C) 5.3 3.5 d Catalytic ... 41.1c 47.1d From [25] kcat between RNase Sa2 and RNase Sa, and to better understand the function of the amino acids involved in catalysis, we analyzed the active sites of RNase Sa2, RNase Sa (2SAR,...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... mRNA and also of its own transcript For this reason, the level of mRNA for the wildtype form of MCPIP and the level of mRNA for IL-1b (Fig 5A, lane 3) were lower in comparison to the level of mRNA ... UTR of IL-6 mRNA [4,17] The presence of such a sequence may account for the degra- MCPIP protein as an RNase dation of both types of IL-1b transcripts, with and without AREs The influence of MCPIP ... overexpression and 25% for siRNA), the overall conclusion from the experiment with fibroblasts is that MCPIP regulates the amount of IL-1b mRNA Involvement of PIN domain of MCPIP in the stability of IL-1b...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... s)1, Km of 0.148 ± 0.028 mm and kcat ⁄ Km of 5.2 · 104 m)1Æs)1 toward shikimate, and a kcat of 7.1 ± 0.7 s)1, Km of 0.182 ± 0.027 mm and kcat ⁄ Km of 3.9 · 104 m)1Æs)1 toward NADP Different from ... profile of HpSDH It is found that the pH optimum of 4686 Fig Temperature profile of HpSDH enzyme activity Fig pH profile of HpSDH enzyme activity HpSDH is between 8.0 and 9.0, and the pH optimum of ... b-sheet, b-turn, and random coil in HpSDH are, respectively, 16.6, 49.2, 1.5, and 32.6% processed by jasco secondary structure estimation software The percentage for random coil of HpSDH is similar...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... sequences of VsEndA (Fig 1) The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA Expression and purification From L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and ... Endonuclease I from V salmonicida and V cholerae Here we report the cloning, expression and purification of the endonuclease I enzymes from the psychrophile V salmonicida (VsEndA) and the mesophile ... characterization of the enzymes, we carried out an analysis of the NaCl requirements and pH optimum of VsEndA and VcEndA The optimum NaCl concentrations for activity were found to be 425 mm for VsEndA and...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose