protein interaction of class b mads box genes from dendrobium moniliforme
Conceptualizing the interaction of class and gender
... Consciousness = a + B1 (Class)< /b> + B2 (Gender) + B3 (Class6< /b> Gender) The coef®cients B1 , B2 , and B3 indicate something about the magnitude of < /b> the effects of < /b> each term in the equation on consciousness The interaction < /b> ... example, a number of < /b> contributors to the ``domestic labor debate'' of < /b> the 1970s (e.g Secombe 1974; Gardiner 1975) argued that the subordination of < /b> women is rooted in the sexual division of < /b> labor in ... turn is to be explained by the fact that the unpaid domestic labor of < /b> women raises the rate of < /b> pro®t by lowering the costs of < /b> reproducing labor power (since part of < /b> the consumption of < /b> workers...
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Báo cáo khoa học: Regulated interaction of endothelin B receptor with caveolin-1 potx
... ETBR from caveolae and ETBR by immunoblotting (Fig 4A) The ETBR expressed in HEK293 cells was also observed as two bands in immunoblotting Fig 4B shows the extents of < /b> caveolin-1 bound to ETBR, ... Fig 5B, while Fig 5C represents the averaged band intensities of < /b> ETBR observed in Fig 5B from five repeated experiments, relative to the band intensity of < /b> total ETBR [band I (full-length isoform) ... membrane solubilization before immunoprecipitation, which allowed the recovery of < /b> ligandfree ETBR, as described later, and the solubilization of < /b> many Triton-insoluble proteins [31] The eluates from...
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Identification and characterisation of a mads box gene from rafflesia cantleyi solms laubach (rafflesiaceae)
... MADS- box proteins such as MADS1 from Populus tomentosa, MPF2 and MPF4 from Physalis pubescens, IbMADS4 from Ipomoea batatas, StMADS11 and StMADS16 from Solanum tuberosum, and SVP and AGL24 from ... and T The classes of < /b> MADS- box genes which control flower formation are known as floral MADS- box genes (Nam et al., 2003) 2.2.1 Floral organ identity genes The best studied plant MADS- box transcription ... designed based on the conserved MADS box of < /b> MADS box genes The primers used were are listed in Table 3.1 PCR reactions were performed using 20 Table 3.1 Degenerate primers used in cloning MADS- box genes...
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Tài liệu Báo cáo khoa học: Three-dimensional structures of thermophilic b-1,4-xylanases from Chaetomium thermophilum and Nonomuraea flexuosa Comparison of twelve xylanases in relation to their thermal stability pdf
... the number of < /b> salt bridges varies between and 12 (Table 6) There is one completely conserved salt bridge between the C-terminal Glu (or Asp) of < /b> b- strand B6 (BAX and BCX have Asp) and Arg of < /b> the ... additional hydrogen bonding with b- strand A5 and the extension of < /b> b- strand B3 interacts with a b- strand B4 BAX also has a longer C-terminal b- strand A4 and a short additional b- strand after that ... the loop between B3 and A5 combined with extended C-terminus of < /b> DTX gives additional hydrogen bonding and hydrophobic Ó FEBS 2003 Thermophilic b- 1,4-xylanases (Eur J Biochem 270) 1407 Table Total...
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Báo cáo khoa học: Spectroscopic investigation of the reaction mechanism of CopB-B, the catalytic fragment from an archaeal thermophilic ATP-driven heavy metal transporter potx
... ATP and Table Binding of < /b> nucleotides to the catalytic fragments of < /b> CPXATPase CopB The interaction < /b> is quantied from apparent binding constants obtained by competitive binding titration of < /b> mant-ATP ... Ras, Ran, Rab, Rap and Rho, which exhibit vibrational uncoupling of < /b> the phosphate resonances and signicant shifts of < /b> the a, b and c absorbance bands, resulting from strong interactions of < /b> phosphate ... bound ATP becomes immobilized by other molecular parts of < /b> the nucleotide, presumably the purine moiety, which apparently protrudes into a binding pocket formed by CopB -B as seen in Fig CopB-B...
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Báo cáo khoa học: Purification and characterization of cathepsin B-like cysteine protease from cotyledons of daikon radish, Raphanus sativus docx
... Alignment of < /b> occluding loop sequences of < /b> plant CBCP from daikon (DDBJ accession number AB377273), A thaliana (DDBJ accession number AF370193), rice (DDBJ accession number AY916493), barley (DDBJ accession ... imbibition From cotyledons obtained days after imbibition, a faint 28 kDa band and additional bands were also detected A clear 28 kDa band and additional bands were observed from cotyledons obtained ... Monopeptidyl carboxypeptidase activity of < /b> CBCP Characterization of < /b> daikon CBCP Table Effects of < /b> various cysteine protease inhibitors on the activity of < /b> CBCP Activities toward Boc-Leu-Arg-Arg-MCA...
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báo cáo khoa học: "Expectation of variance due to mitochondrial genes from several mating designs" ppsx
... Evolution of < /b> genes and proteins, 147-164, Sinauer Associates, Sunderland, Mass BELL B. R., Mc DANIEL B. T., R O.W., 1985 Effect of < /b> cytoplasmic inheritance on producOBISON tion traits of < /b> dairy cattle ... estimation of < /b> the others components, both in terms of < /b> sampling variance (tabl 3) and of < /b> actual estimate This is illustrated by reanalyzing the data of < /b> R et al., (1985a) The results, given in table 4, ... (AviSE & RSOT U , ANSMAN L 1983 ; BO & BONHOMME, 1986) cytoplasmic effects includes differences in traits from N O OBIS et KIN S ERE , OUCHBERRY , RUMBY (B 1960 ; B & T 1966 ; R al., O P 1981 ;...
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Báo cáo y học: "A scale of functional divergence for yeast duplicated genes revealed from analysis of the protein-protein interaction network" doc
... classification of < /b> proteins for the prediction of < /b> cellular function from a protein-< /b> protein interaction < /b> network Genome Biol 2003, 5:R6 Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry ... Table Summary of < /b> the behaviors of < /b> the 41 duplicated genes Classification behaviors Number of < /b> duplicated pairs I Same class,< /b> same biological process 26 (63%) II Different classes, same biological ... (48) BMH2 BMH1 + + BOI1 BOI2 + + Energy derivation by oxidation of < /b> organic compounds, polysaccharide metabolism, carbohydrate metabolism (6) Nuclear organization and biogenesis (22), nucleobase...
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Tài liệu Báo cáo khoa học: ¨ Induction of Kruppel-like factor 4 by high-density lipoproteins promotes the expression of scavenger receptor class B type I pptx
... real-time PCR (B) Protein < /b> levels of < /b> KLF4 were determined by western blot (C) mRNA levels of < /b> hSR-BI were determined by real-time PCR (D) Protein < /b> levels of < /b> hSR-BI were determined by western blot The ... immunoreactive bands were visualized using diaminobenzidine (DAB) (Boster Biological Technology, Wuhan, Hubei, China) The following antibodies were used: rabbit SRBI polyclonal antibody (1 : 1000, Abcam, ... Mutant probe, competition with mutant cold probe (200-fold excess concentration); KLF4 Ab, supershift group by KLF4 antibody (B) Recruitment of < /b> KLF4 to the binding element of < /b> the SR-BI promoter...
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Tài liệu Báo cáo khoa học: Interaction of the small heat shock protein with molecular mass 25 kDa (hsp25) with actin doc
... other by mAu Ó FEBS 2003 Actin and small heat shock protein < /b> (Eur J Biochem 270) 895 Fig Fluorescence spectra of < /b> 140 lM ANS in the absence of < /b> added proteins (1) and in the presence of < /b> 2.25 lM of < /b> ... stopped by the addition of < /b> phenylmethanesulfonyl fluoride, followed by boiling in the sample buffer The samples obtained were subjected to quantitative SDS gel electrophoresis (B) Time course of < /b> the ... (Fig 4B, curve 1) If actin heated at 60 °C was subjected to trypsinolysis under the same conditions, the band of < /b> actin disappeared in the first 15–30 s (Fig 4B, curve 2) and a number of < /b> faint bands...
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Tài liệu Báo cáo Y học: A family of expressed antifreeze protein genes from the moth, Choristoneura fumiferana ppt
... UTR isoform class < /b> based on its nucleic acid sequence and the potential size of < /b> its mRNA Molecular modeling of < /b> this new, putative isoform, based on the NMR-derived structure of < /b> the 337 isoform, ... approximately 17 bands (consistent with the number of < /b> hybridizing plaques/genome in the library screens) of < /b> various intensities could be resolved in the SstI, HindIII and EcoRI digests of < /b> the DNA from individual ... copies of < /b> the gene hybridizing with the 337 probe (one of < /b> which would correspond to Afp-Lu1) and two copies hybridizing to the Ó FEBS 2002 Antifreeze protein < /b> genes in Choristoneura (Eur J Biochem...
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Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
... coefficient P(x), number of < /b> correct predictions in class < /b> x/number total predictions in class < /b> Q(x), number of < /b> correct predictions in class < /b> x/number total observed in class < /b> x 60 Number of < /b> Proteins Contact ... values of < /b> reliability index, by comparing with the presently available data base of < /b> interacting complexes the accuracy may be biased by the lack of < /b> knowledge of < /b> all the possible protein < /b> interactions ... determined by its position in the Ó FEBS 2002 1358 P Fariselli et al (Eur J Biochem 269) Table Scoring the efficiency of < /b> the neural network-based predictor Q2, number of < /b> correct predictions/number of < /b> total...
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Tài liệu Báo cáo Y học: Proteolysis of bovine b-lactoglobulin during thermal treatment in subdenaturing conditions highlights some structural features of the temperature-modified protein and yields fragments with low immunoreactivity pptx
... one of < /b> the goals of < /b> this work was to take advantage of < /b> the interplay of < /b> treatment conditions and enzyme action to produce fragments of < /b> sizable mass, but unable to be recognized by specific antibodies ... of < /b> beta-structural domains of < /b> beta-lactoglobulin liberated by limited proteolysis J Prot Chem 12, 613–625 14 Madsen, J.S., Ahmt, T.Ø., Otte, J., Halkier, T & Qvist, K .B (1997) Hydrolysis of < /b> beta-lactoglobulin ... Ó FEBS 2002 1368 S Iametti et al (Eur J Biochem 269) Table Molecular parameters for large-sized fragments obtained from hydrolysis of < /b> BLG Fragments were obtained by size-exclusion HPLC of < /b> digests...
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Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... the ATP levels, B subtilis GlnK was shown in vitro to be soluble or membrane-bound: mm ATP caused almost full solubilization of < /b> GlnK [12] In wild-type B subtilis, TnrA was shown to bind specifically ... Germany) equilibrated with 10 column volumes (10 · 0.2 mL) of < /b> buffer B, with subsequent washing four times with five volumes of < /b> the same buffer Proteins were eluted with buffer E (buffer B supplemented ... remains bound to GlnK and is therefore protected from proteolysis This suggests a role of < /b> AmtB in dissociation of < /b> the GlnK–TnrA complex A possible regulatory role of < /b> AmtB proteins has been suggested...
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Báo cáo khoa học: The F13 residue is critical for interaction among the coat protein subunits of papaya mosaic virus doc
... Erickson JW, Bancroft JB & Horne RW (1976) The assembly of < /b> papaya mosaic virus protein < /b> Virology 72, 514–517 Abouhaidar M & Bancroft JB (1978) The initiation of < /b> papaya mosaic virus assembly Virology ... supernatant (disks) of < /b> the purified proteins was incubated with 165 fmol of < /b> an RNA probe labeled with c-32P made from an 80 nucleotide RNA transcript from the 5¢-end of < /b> PapMV The disks of < /b> CP6-215 and ... M E Laliberte Gagne et al F13 critical for interaction < /b> among the CP subunits A Fig EMSA with high-speed supernatant of < /b> recombinant disks obtained from the disruption of < /b> the NLPs by use of < /b> the...
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Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... determined by band fitting of < /b> the absorbance spectrum of < /b> Fig 4A, are shown in Fig and Table From Table 2, the sum of < /b> individual amide I¢ intensity and the intensity percent of < /b> each peak from human Ó FEBS ... implied that PKIb was a thermostable protein < /b> and part of < /b> the protein < /b> may coprecipitate with other denatured proteins Starting from the L of < /b> bacterial culture, 1.52 mg of < /b> purified PKIb was obtained with ... regulation of < /b> the PKI and PKI isoforms of < /b> the inhibitor protein < /b> of < /b> the cAMP-dependent protein < /b> kinase J Biol Chem 272, 20011–20020 Byler, D.M & Susi, H (1986) Examination of < /b> the secondary structure of < /b> proteins...
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Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... scatter contribution was derived from acrylamide titration of < /b> a vesicle blank Ksv, which is a measure of < /b> the accessibility of < /b> Trp to acrylamide, was obtained from the slope of < /b> the plots of < /b> F0 ⁄ F ... study of < /b> the interaction < /b> of < /b> penetratin peptides with model membranes Eur J Biochem 269, 2918–2926 Lobley A, Whitmore L & Wallace BA (2002) DICHROWEB: an interactive website for the analysis of < /b> protein < /b> ... peptide–membrane interaction,< /b> and combined analysis of < /b> the data obtained provides insights into HGV ⁄ GBV-C–membrane interaction < /b> Results The E2 peptides synthesized are amphiphilic because of < /b> the...
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Báo cáo khoa học: Interaction of human stefin B in the prefibrillar oligomeric form with membranes Correlation with cellular toxicity doc
... composed of < /b> loosely bound oligomeric blocks can be seen and, at pH 3.3 (Fig 1B) , necklacelike structures built from basic ellipsoid blocks (similar to protofibrils) are observed At pH 7.3, oligomers of < /b> ... stefin B prefibrillar oligomers of < /b> vesicles made of < /b> acidic phospholipids resembles pore formation by A -b [27] and liposome permeabilization of < /b> a-synuclein [43] The toxic activity exerted by prefibrillar ... Loosely bound vesicles were eluted from the chip by three injections of < /b> 100 mm NaOH Unspecific binding sites were blocked by one injection of < /b> 0.1 mgÆmL)1 bovine serum albumin For the binding experiment...
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Báo cáo khoa học: Flexible nets The roles of intrinsic disorder in protein interaction networks potx
... number of < /b> protein< /b> protein < /b> interactions (inter.) only Proteins are ordered by percentage of < /b> disorder BIND, the Biomolecular Interaction < /b> Network Database (http://bind.ca/) [201] DIP, the Database ... about 93% of < /b> the total number of < /b> proteins, only about 21% of < /b> them are essential to cell survival By contrast, only 0.7% of < /b> yeast proteins are known to have more than 15 links (i.e., hubs), but ... regions of < /b> intrinsic disorder One example of < /b> an ordered hub, calmodulin, makes use of < /b> a flexible hinge to facilitate binding diversity Each of < /b> these classes and their roles in protein< /b> protein < /b> interaction...
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Báo cáo Y học: Interaction of the anterior fat body protein with the hexamerin receptor in the blowfly Calliphora vicina pot
... strong interaction < /b> between AFP and ABP130 and ABP96, but no interaction < /b> with ABP64 (Table 1) Ó FEBS 2002 Calliphora anterior fat body protein < /b> (Eur J Biochem 269) 957 Table Interaction < /b> of < /b> AFP ... were identified by screening the prey library with ABP130 (once) or ABP96 (twice) as baits We also examined the ability of < /b> AFP to interact with ABP130, ABP96, and ABP64 in the two-hybrid assay We ... most detailed investigations of < /b> fat body proteins has been the metabolism of < /b> the storage protein < /b> arylphorin, which belongs to the class < /b> of < /b> hexamerins [5–9] These proteins are synthesized in a...
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