... the properties of the three protein classes, with the main focus being on RNAchaperones and annealer proteins.Specifically binding proteinsA specific protein cofactor binds to its RNA targetthrough ... mRNA-binding protein hnRNP A1 [61], theXenopus laevis protein X1rbpa [54], the trypanosomeguideRNA-binding protein RBP16 [62], and theEscherichia coli protein StpA [63], an inverse or miss-ing correlation ... easily gain access to theRNA protein interface to increase the dissociationprocess. The promiscuity often reported for proteinsM. Doetsch et al. Transient RNAprotein interactions in RNA foldingFEBS...
... epidemics by pyrrolizidine alkaloids have occurred in thedeveloping world. In the United States, poisonings, includingdeaths, have been associated with use of herbal teas containingcomfrey (Huxtable, ... main breakdown com-pound of glucobrassicin, a glucosinolate that is present in largeamounts in vegetables of the Brassica genus, including broc-coli, and gives rise to the potent Ah binder indole ... 4-methylcatechol, methylhydrazine, monocrotaline, pentanal methylformylhydrazone, petasitenine, quercetin, reserpine,safrole, senkirkine, sesamol, symphytineNoncarcinogens:N = 34Atropine, benzyl alcohol,...
... analysing for contaminants in water, that samples are taken into a preparedvessel in order to minimise contamination from environmental sources. When sampling in domesticpremises, drinking water ... leading toincreased concentrations in the water in the domestic system.3.18 Flushing of the mains distribution system to remove the contaminated water resulted in the disturbanceof old mains ... 50: Usual intakes of aluminium from food and water and potential intakes from medicines (mg/kg bw/day) 181Table 51: Summary of margin of safety (MoS) for aluminium after the pollution incident...
... 53–56.34 Tollin G (1995) Use of flavin photochemistry to probeintraprotein and interprotein electron transfer mecha-nisms. J Bioenerg Biomembr 27, 303–309.Supporting informationThe following supplementary ... structure of the cobalamin-binding domainHomocysteineMethionineCoMethylcob(III)alaminCob(I)alaminPrimaryturnovercycleHCH3H4folateAdoHycCob(I)alaminCob(II)alaminH4folateCoNADPHAdoMete–FADFMNNADPHNADP+e–ReactivationCoFig. ... CH3-H4-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark grey circle).hMS is inactivated approximately every 200–1000 catalytic turnovers [owing to...
... determine the calcium-binding domain ofthe protein. (A) Schematic drawing of the recombinant proteins. SixGST-fused recombinant proteins containing the three distinctivedomains of tandem repeat ... matrix proteinsinduce aragonite crystals [4–7]. We therefore exam-ined proteins contained in aggregates within theotolith matrix and identified a protein contained in the HMW protein glycosaminoglycan ... Pi, and specificbinding of the antibody was detected by incubating thesections in a solution of 10 mm diaminobenzidine in 10 mm NaCl ⁄ Pi.H. Tohse et al. A novel proteinin the otolith matrix...
... neuroepithelialcells into astrocytes, by binding and hyper activatingthe promoter of glial fibrillary acidic protein (GFAP). In parallel, BMP signals lead to enhanced expressionof ID proteins, which in turn bind ... protein- inducedT. U. Wagner BMP signaling in stem cellsFEBS Journal 274 (2007) 29682976 ê 2007 The Author Journal compilation ª 2007 FEBS 2975 MINIREVIEWBone morphogenetic protein signaling ... of ligand-receptor affinity ) usually bybinding to ligand dimersDimeric secreted proteins such as noggin,chordin, follistatin and GDF3 bind and therbyinactivate BMP dimers [16]Cell membrane...
... structure ofheparin (file 1hpn [35]).Amino acids known to be part of the heparin-binding site in these three crystallized protein heparin complexes weregiven as starting point for the docking search ... observation thatheparin does not inhibit FVa inactivation by recombinantAPC variants that are unable to bind heparin supports themechanism in which heparin binding to the basic cluster in APC is responsible ... deficient in heparin bindingTo verify that the inhibition of APC-mediated FVainactivation by heparin is related to the ability of heparinto bind APC, we used recombinant mutants of APCharboring...
... listed in Fig. 4). Again both nfeAFP2 and nfeAFP6 wereexpressed with C-terminal lysines. We also expressednfeAFP6minusLys66 (nfeAFP6DLys; SP group) toexamine the effect of the C-terminal lysine ... solution was placed in thecooling pot ()7 °C) of the instrument; (b) the frosty probewas put manually into the supercooled sample to initiatewater freezing; (c) the increase in solution temperaturecaused ... (1996) A natural variant of type I antifreeze protein with four ice-binding repeats is a particularly potentantifreeze. Protein Sci 5, 1150–1156.29 Leinala EK Davies PL Doucet D Tyshenko MGWalker...
... ofperishable foods. P24 Understanding interactions of food (ingredients) and processing at themolecular and cellular levelHow can we understand better what is happening within the food during food processing, ... products or in thedevelopment of new packaging formats adapted to specific consumersegments. A synergetic effect in maintaining food quality and increasingsustainability is obtained by means ... re-construction of the food chain from the consumer point of view, the reversed food chain thinking.This reconstruction of the food chain from the consumer perspective served in the following as the reference...
... rapamycin;p70S6K, p70 ribosomal protein S6 kinase; PDK1, 3-phosphoinositide-dependent protein kinase; PKB, protein kinase B; PP2A, protein phosphatase 2A.*Present address: GlaxoSmithKline Biologicals,Research ... shownschematically in Fig. 8, based on the available data in theliterature and integrating the results obtained in the presentstudy.ACC is known to be activated by incubating hepatocyteswith certain amino ... Hemmings, B.A. &Thomas, G. (1999) Protein kinase B localization and activationdifferentially affect S6 kinase 1 activity and eukaryotic translationinitiation factor 4E-binding protein 1...
... Low-Carb Diets and the Atkins Revolution Industry Opinion Survey, April 2004 Business Insights According to Janine Wilson, an independent nutritionist based in the UK: “The rising levels of obesity ... marketing streams based on a firm understanding of the major consumer health concerns influencing purchasing decisions and eating habits. Identifying the main health-related issues influencing ... extremely interesting to note that this trend is not expected to continue with such high levels of success. Kids remain the focus in the food and drinks industry – even when it comes to healthy food...
... thepresent study indicate that adipophilin protein expression in muscle isinvolved in maintaining insulin sensitivity.AbbreviationsAdfp, adipophilin; CLB, classical lysis buffer; FA, fatty ... is implicated in insulin resistance. As shown in the present study,palmitic acid indeed impaired insulin signaling in C2C12 cells, which is in line with numerous studiesaddressing the effect ... in the glycolysis, were reduced in the palmiticacid-treated cells. Additionally, the protein level ofprohibitin was increased in palmitic acid-treated cells.Prohibitin is involved in the inhibition...
... Genetic Manipulation of DNA and Protein – Examples from Current Research 12DNA binding domain and a domain for its interaction with the silencing linker protein called Net1. Net1 recruits ... specific alteration of amino acid residues in proteins, gene fusions, cysteine- and alanine-scanning mutagenesis, recombineering, cloning by “capturing” large segments of DNA, transposable elements, ... discussed in more detail later. Crystal structures of Terminator proteins: The first crystal structure of a terminator apoprotein, namely that of RTP, showed that the protein was a symmetrical winged...
... glycine, l-alanine,l-proline, l-lysine, l-serine, b-alanine, taurine, sarcosine,dimethylglycine, glycine betaine, trimethylamine N-oxide,and cytidine 2Â-3Â cyclic monophosphate were also obtainedfrom ... stabilize proteins[3,4], but they also induce refolding of misfoldedproteins [5–8] and remove protein aggregation [9–12].Mechanisms of protein osmolyte interactions, theeffect of osmolytes on protein ... alanine, proline, serine, lysine, b-alanine andtaurine) that have no significant effects on both DGD°and kcat. (b) Class II represents methylamines (sarco-sine, dimethylglycine, betaine,...
... contains three TPR motifsforming the docking site for Hsp90-bound preproteins. AtToc12 (12)exposes a J-domain (J) into the intermembrane space and has arole in anchoring Hsp70, thereby assisting ... accumulation of many proteins involved in photosyn-thesis (termed ‘photosynthetic proteins’), suggestingthat both atToc33 and atToc159 are involved in theimport of photosynthetic proteins. However, ... photosyn-thetic preproteins (down-regulated in the mutants) isunclear. However, many proteins that are not involved in photosynthesis (termed ‘housekeeping proteins’)accumulate normally in both ppi1...