protein bovine serum albumin
Enzymes in the Environment: Activity, Ecology and Applications - Chapter 11 ppsx
Ngày tải lên :
11/08/2014, 15:20
... chemistry of human serum albumin Nature 358: 209–214, 1992 DC Carter, JX Ho Structure of serum albumin Adv Protein Chem 45:155–203, 1994 A Kondo, K Higashitani Adsorption of model proteins with wide ... contact between a single protein molecule and the clay surface at different pH and ionic strengths Conformational Changes on Adsorption of a Soft Protein, Bovine Serum Albumin a Description of ... involved in interactions of proteins with hydrophilic mineral surfaces An example is given by the higher affinity of a hydrophobic methylated derivative of bovine serum albumin (BSA) for the hydrophilic...
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Tài liệu Báo cáo khoa học: Crystal structures of a bacterial 6-phosphogluconate dehydrogenase reveal aspects of specificity, mechanism and mode of inhibition by analogues of high-energy reaction intermediates pdf
Ngày tải lên :
19/02/2014, 05:20
... incubating protein with ligands in a : ratio Complex III was prepared by mixing the protein, NADP+ and inhibitor in a ratio of : : 10 Crystals were grown in hanging drops constructed from lL of protein ... > ⁄ Mosaicity (°) R-sym (%)a ˚ Wilson B (A2) No of protein residues ⁄ solvent molecules R-work b ⁄ R-free c (%) ˚ Average B (A2) Overall ⁄ protein ⁄ solvent rmsd ˚ Bond lengths (A) ⁄ bond angles ... Systematic analysis of conformational domain motions in proteins from conformational change: new results on citrate synthase and T4 lysozyme Proteins 30, 144–154 Hanau S, Dallocchio F & Rippa M...
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Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
Ngày tải lên :
20/02/2014, 03:20
... 8.0 Bound proteins were eluted by a linear gradient from to m NaCl in the same buffer Fractions containing PelB were pooled and concentrated (Filtron Technology Corp.; 30-kDa cut-off) Protein concentrations ... brisolvens from the bovine rumen J Gen Microbiol 131, 20532058 33 Wojciechowicz M, Heinrichova K & Ziolecki A (1982) An exopectate lyase of Butyrivibrio brisolvens from the bovine rumen J Gen ... treatment of the cell-free extract, followed by anionexchange chromatography, during which the protein was eluted at 0.6 m of NaCl Analysis of PelB by gel ltration resulted in a peak with an...
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Báo cáo khoa học: Assembly and molecular mode of action of the Helicobacter pylori Cag type IV secretion apparatus doc
Ngày tải lên :
06/03/2014, 00:21
... TL (2006) Protein Protein interactions among Helicobacter pylori Cag proteins J Bacteriol 188, 4787–4800 Mossey P, Hudacek A & Das A (2010) Agrobacterium tumefaciens type IV secretion protein VirB3 ... CagA-positive strains, was shown to encode proteins with sequence similarity to Agrobacterium tumefaciens Vir proteins, and it was further demonstrated that these proteins are necessary for inducing ... compilation ª 2011 FEBS 1205 Cag protein functions W Fischer Table Overview of characteristics and functions of cagPAI-encoded proteins Gene designations and protein sizes are in accordance with...
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Báo cáo khoa học: Structural and thermodynamic insights into the binding mode of five novel inhibitors of lumazine synthase from Mycobacterium tuberculosis pptx
Ngày tải lên :
07/03/2014, 11:20
... lacks four direct contacts with protein atoms made by the hydroxyl groups of the ribityl chain in the other structures Moreover, several direct contacts with the protein chain by the nitrogen atoms ... methods for building protein models in electron density maps and the location of errors in the model Acta Crystallogr A47, 110119 Kleywegt GJ & Jones TA (1998) Databases in protein crystallography ... (%) Overall I r Rsym overall (%)a Wilson plot (A2) Renement Resolution range (A) Non hydrogen protein atoms Non hydrogen inhibitor atoms Solvent molecules Solvent ions Rcryst overall (%)b Rfree...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx
Ngày tải lên :
07/03/2014, 16:20
... physiological role of the PAT2 transporter is currently not known We previously reported the PAT2 protein expression pattern in the mouse brain [10] and demonstrated that it is highly expressed ... possible insertion of PAT2 into the neuronal plasma membrane and we proposed PAT2 as a candidate protein for the still missing Na+-independent low affinity glycine transport system in the central ... other tissues than the nervous system, cellular and subcellular expression pattern of the PAT2 protein have not been examined so far, although the mRNA is expressed in various organs such as...
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Báo cáo khoa học: Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23 A unique type of cellulase among Bacillales ppt
Ngày tải lên :
08/03/2014, 02:21
... For analysis of bound proteins, the last pellets were eluted using 0.2 M glucose, M NaCl, 1.5 M urea or H2O before loading onto SDS–polyacrylamide gels (10% acrylamide) for protein analysis and ... buffer and gently re-suspended for removal of unbound proteins A final wash was performed with 10 mM phosphate buffer pH 6.0 Elution of bound proteins was achieved by addition of vol water, followed ... terminator was found after the stop codon of cel48C, acting as a signal structure for protein synthesis termination The protein deduced from cel48C contained 1091 amino acids and showed a predicted molecular...
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Báo cáo khoa học: The twin-arginine translocation (Tat) systems from Bacillus subtilis display a conserved mode of complex organization and similar substrate recognition requirements doc
Ngày tải lên :
16/03/2014, 04:20
... indispensable for productive protein translocation Several of the mutated precursor proteins associate strongly with the membrane in the absence of efficient export It is possible that the proteins are associating ... References Cline K, Ettinger WF & Theg SM (1992) Protein- specific energy requirements for protein transport across or into thylakoid membranes Two lumenal proteins are transported in the absence of ... C, Berks BC & Palmer T (2000) TatD is a cytoplasmic protein with DNase activity No requirement for TatD family proteins in sec-independent protein export J Biol Chem 275, 16717–16722 10 Jack...
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Báo cáo khoa học: The sulfur atoms of the substrate CoA and the catalytic cysteine are required for a productive mode of substrate binding in bacterial biosynthetic thiolase, a thioester-dependent enzyme doc
Ngày tải lên :
16/03/2014, 04:20
... mitochondrial trifunctional protein and its inactivation study for medicine development Biochim Biophys Acta 1784, 1742–1749 33 Fersht A (1999) Structure and Mechanism in Protein Science: A Guide ... Automated protein model building combined with iterative structure refinement Nat Struct Biol 6, 458–463 46 Jones TA, Zou J, Cowan SW & Kjeldgaard M (1991) Improved methods for building protein ... seen that the binding mode is the same as that for OPP; furthermore, the structural changes of the protein part described for the OPP complex are seen as well in the Ac-OPP complex Due to low occupancy...
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Báo cáo khoa học: Structures and mode of membrane interaction of a short a helical lytic peptide and its diastereomer determined by NMR, FTIR, and fluorescence spectroscopy pdf
Ngày tải lên :
17/03/2014, 11:20
... barley serine proteinase inhibitor Protein Eng 2, 27–38 32 Laskowski, R.A., MacArthur, M.W., Moss, D.S & J.M.Thornton (1993) PROCHECK: a program to check the stereochemical quality of protein structure ... constants in proteins Biochem Biophys Res Commun 113, 967–974 31 Nilges, M., Gronenborn, A.M., Brunger, A.T & Clore, G.M (1988) Determination of three-dimensional structures of proteins by simulated ... single-quantum NMR spectroscopy of aqueous solutions J Biomol NMR 2, 661–665 27 Wuthrich, K (1986) NMR of Proteins and Nucleic Acids John Wiley & Sons, New York 28 Davis, D.G & Bax, A (1985) Assignment...
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Báo cáo khoa học: Peptides that bind the HIV-1 integrase and modulate its enzymatic activity – kinetic studies and mode of action pptx
Ngày tải lên :
22/03/2014, 16:21
... (1999) Miniaturized proteins: the backbone cyclic proteinomimetic approach J Mol Biol 292, 421429 32 Murray JK & Gellman SH (2007) Targeting protein protein interactions: lessons from p53 MDM2 Biopolymers ... protein: characterization of the karyophilic properties and inhibition of Rev-induced gene expression Biochemistry 44, 1155511566 31 Kasher R, Oren DA, Barda Y & Gilon C (1999) Miniaturized proteins: ... tetramers and associates with LEDGF p75 protein in human cells J Biol Chem 278, 372381 45 Turlure F, Devroe E, Silver PA & Engelman A (2004) Human cell proteins and human immunodeciency virus...
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Báo cáo khoa học: Common mode of DNA binding to cold shock domains Crystal structure of hexathymidine bound to the domain-swapped form of a major cold shock protein from Bacillus caldolyticus pot
Ngày tải lên :
23/03/2014, 09:21
... structure the two protein chains form a swapped dimer with two globular functional units which are composed of residues 1–35 from one protein chain and residues 38–65 from another protein chain (Figs ... DNA binding is dominated by stacking interactions between nucleobases and aromatic protein side chains for both proteins Based on this observation and on binding assays using a set of heptapyrimidines, ... amphipathic protein surface Several residues participating in ligand binding are located in regions designated as RNP motifs I and II, which can also be found in other RNA-binding proteins [26,27]...
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Báo cáo khoa học: Contribution of a central proline in model amphipathic a-helical peptides to self-association, interaction with phospholipids, and antimicrobial mode of action ppt
Ngày tải lên :
23/03/2014, 10:21
... translocation of proteins across membranes Biochem J 347, 601–612 56 Nouwen N, Tommassen J & de Kruijff B (1994) Requirement for conformational flexibility in the signal sequence of precursor protein J ... antibacterial peptides Curr Drug Targets 3, 101–106 19 Otvos L Jr (2005) Antibacterial peptides and proteins with multiple cellular targets J Pept Sci 11, 697–706 20 Park CB, Yi KS, Matsuzaki K, Kim ... alpha-helices J Mol Biol 284, 867–873 Lau SY, Taneja AK & Hodges RS (1984) Synthesis of a model protein of defined secondary and quaternary structure Effect of chain length on the stabilization...
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Báo cáo Y học: Isolation, enzymatic properties, and mode of action of an exo-1,3-b-glucanase from Trichoderma viride doc
Ngày tải lên :
24/03/2014, 04:21
... lane 2, protein standards – phosphorylase a (98 kDa), BSA (67 kDa), ovalbumin (45 kDa), a-chymotrypsin (25 kDa) had a specific activity of 63 U:mg21 (Table 1) In SDS/ PAGE analysis the protein ... Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227, 680–685 32 Lowry, O.H., Rosenbrough, N.J., Farr, A.L & Randall, R.J (1951) Protein measurements with ... preparation was loaded on a DEAE-Sephadex column (50 Â 200 mm) equilibrated with buffer A and bound protein was eluted with M NaCl in the same buffer Following concentration to 25 mL on an Amicon...
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Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx
Ngày tải lên :
30/03/2014, 15:20
... 3H-labelled UTP (0.5 lL of 50 CiÆmmol)1, mCiÆmL)1) and different concentrations of antibiotics Protein biosynthesis was assayed by incubating 10 lL of plasmolyzed cells in a total volume of 50 ... solution of trichloroacetic acid in water was added and the mixtures were incubated at °C for 30 (for protein biosynthesis assays, after the addition of trichloroacetic acid, samples were preincubated ... few minutes, in the same manner as the rifampicin control, while no effect is observed on DNA or protein synthesis Thus, the specificity of action observed with purified enzymes (Table 1) is confirmed...
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Báo cáo Y học: Exploring the primary electron acceptor (QA)-site of the bacterial reaction center from Rhodobacter sphaeroides Binding mode of vitamin K derivatives pptx
Ngày tải lên :
31/03/2014, 21:21
... designed for the docking of small to medium sized organic molecules into protein binding sites [36] During the docking procedure, the protein is considered as rigid, whereas the ligand conformation is ... resulting from the response of the protein to the QA-reduction Hence, these absorption bands are indicative for the interaction between the quinone and the protein binding pocket Therefore, structural ... identical leading to the same protein environment formed predominantly by the side chains of HisM219, IleM223, MetM262 and IleM265 (Table 3) We have constructed hypothetical protein- ligand complexes...
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stenersen - chemical pesticides - mode of action and toxicology (crc, 2004)
Ngày tải lên :
03/04/2014, 12:11
... 2.1.7 Strong electrophiles, alkalis, acids, oxidants, or reductants that destroy tissue, DNA, or proteins 18 2.2 How to measure toxicity 18 2.2.1 Endpoints 18 2.2.1.1 Endpoints ... convince me that the fly has increased the nutritional value of the soup by adding vitamins and proteins, I will not have the soup Our comprehension of food quality and a pleasant environment ... and Klaassen (2001) 2.1.1 Enzyme inhibitors The toxicant may react with an enzyme or a transport protein and inhibit its normal function Enzymes may be inhibited by a compound that has a similar,...
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