... cleft, the
putative catalytic zinc being at thebottomofthe cleft
and a lobe containing the structural zinc at the mouth
of the cleft and thus exposed to the solvent [8]. Grad-
ual depletion ofthe ... Novagen.
Expression of Pt-gdh under the control of araB promoter
was induced for 4 h at 30 °C by the addition of 0.2% ara-
binose when the A
600
of the growing culture reached 0.5.
The cells from a 1-L ... tested, i.e. from 0.05 to 1 mm. The influence
of Zn
2+
on the pH stability of GdhA is most evident
after incubation (1 h, 55 °C) at pH 3.5, where, in the
presence ofthe metal ion at 0.1 mm, there was...
... c, with contributions fromthe formyl groups
and fromthe geranyl side chain expected from heme a and
a
3
. In addition to the signals ofthe hemes, the reorgan-
ization ofthe polypeptide backbone ... and
on this basis the contributions ofthe reorganization of the
polypeptide backbone, of individual amino acids and of the
hemes c, a and a
3
upon electron transfer to /from the redox
active centers ... particular problem ofthe assignment in the difference
spectra is the superposition of signals from different
constituents ofthe oxidase, which can lead to the possibility
of multi component...
... ofthe structure of NADP
+
-bound FeR
revealed that the nicotinamide moiety of NADP
+
faces the re-side ofthe isoalloxazine ring, and that the
2¢-P-AMP moiety is held in the groove between the ... direction along the b10 ofthe other mono-
mer, forming an anti-parallel intermolecular b sheet
(Fig. 2A). The subsequent residues ofthe loop (Ala7–
Pro13) turn into the interior ofthe dimer interface,
and ... fulgidus
FeR is one ofthe candidates for the ferric ion-binding
residue based on the structure ofthe mercury-bound
derivative, although a ferric ion was not found at the
site of either the native or...
... allowing
the assessment ofthe patient’s QoL both fromthe parent’s
and fromthe patient’s perspective. The possibility to assess
QoL from different perspectives is a promising character-
istic of ... mainly consists of items fromthe sub-
domains IRA and TA, which together form the Risk avoid-
ance domain. High loadings ofthe second factor came almost
exclusively fromthe EC domain. The third ... 10.1007/s12402-011-0066-y
also for the change from baseline. The same was found to
be true for the Comfort domain regarding the correlation of
the domain with the total score. The Achievement domain,
the Satisfaction...
... ongoing initiatives in
the FATF and the OECD, the Report ofthe OFCs Working Group ofthe
Financial Stability Forum (FSF) has made recommendations to enhance OFCs’
observance of international standards ... characterized by the high mobility of funds and the
rapid development of new payment tools. To secure the benefits ofthe
international financial system, we, the Finance Ministers ofthe G-7 countries, ... programs and the integrity ofthe IFIs
themselves. Thus, we urge the IMF and the World Bank to continue to conduct
an authoritative review of their financial procedures and controls and those of
recipients,...
... presenting the pulse of Src activity in the plane
of the inactive and active fractions, s
i
and s
1
. If the
duration ofthe stimulus exceeds the critical value, the
trajectory in the (s
i
, ... 2009 The Authors Journal compilation ª 2009 FEBS
parameters and, for clarity of exposition, we present
the analysis ofthe Src cycle in terms of Eqns (6–8).
Intrinsic regulatory propertiesofthe ... steady states ofthe Src cycle. When there are three
intersections, the steady state O
1
at the lower branch of
the Z-shaped curve (i.e. low Src activity) and the state
O
3
at the upper branch...
... analyses ofthe predicted
amino acid sequence showed that the C-terminal half
of the RepA ofthe pIT3 plasmid is sequence-similar to
the helicases ofthe phage-encoded superfamily III pro-
teins. The ... comparable
with the two-metal-ion mechanism of both RNA and
DNA synthesis [17].
In a previous study, we reported the findings of an
analysis ofthe complete sequence ofthe cryptic plas-
mid pIT3 isolated from ... is located at the
bottomofthe Zn-stem). However, a MD simulation
on the Rep245 model showed that the absence of
the two disulfide bridges did not affect the overall
protein fold. The Zn-binding...
... transfer
from CDH to cytochrome c, Phe166, which is located in the cytochrome
domain and approaches one of propionates of heme, was mutated to Tyr,
and the thermodynamic and kinetic propertiesofthe ... spectra of heme in CDH (A) and cytochrome c
(B) for comparison ofthe isosbestic points. The spectra ofthe oxi-
dized (solid line) and reduced (dashed line) forms are compared in
the range of 450–650 ... This
was expected fromthe thermodynamic result that the
redox potential of heme in F166Y was lower than that
of WT. However, when the electron transfer rate k
(s
)1
) and the driving force DG...
... years ago
and supports the notion of a universal occurrence of
hemocyanins in the Myriapoda [15,16]. At least three
hemocyanin subunits were present at the time of divergence
of the Chilopoda and ... the determination ofthe N-terminal sequences, C.
Hunzinger for the MALDI-TOF analyses, C. Bache and J. Hermanns
for their help with the cloning experiments, and J. R. Harris for
correcting the ... kDa, the isoelectric
points (pI) range from 5 to 6.5 (Fig. 2). MALDI-TOF
analyses of tryptic peptides show that the spots represent
four distinct subunit types, as deduced fromthe pattern of
identical...
... was good, for the owners both of the
Negro team and the local team. The attendance for the annual game was
often the highest ofthe year.
✶
Parallel with serious Negro ball were the black clown ... Introduction
Jerry Malloy 1 The Birth ofthe Cuban Giants:
The Origins of Black Professional Baseball
Lee Lowenfish 15 When All Heaven Rejoiced: Branch
Rickey and the Origins ofthe Breaking of
the Color Line ... that imitated the standards ofthe white
community that rejected them, while maintaining the unique African
American heritage that was the root cause of that exclusion. In the birth
of the Cuban...
... ielded from solution by
the rest ofthe protein. There is no correlation between the
complex stability and the rate of its formation.
The Ni(II) for Cu(II) exchange rates f or peptides are o f
the ... the
rapid formation ofthe Cu(II) species, and there is little
assistance fromthe buffer components. There is no accel-
eration for DAHKam, compared to DAHam, in accord-
ance with the lack of ... albumins. On the other hand, the direct
thermodynamic and kinetic characterization of Ni(II)
binding at site I in HSA and BSA was obtained. These
data can be very useful in further studies ofthe toxicolo-
gically...
... inserted between the third letter of one codon and
the first letter ofthe following codon.
Molecular modeling of the
C. sepium
b-amylase
The amino-acid sequence ofthe b-amylase from C. sepium
exhibits ... built for the b-amylase
from C. sepium using the X-ray coordinates ofthe soybean
b-amylase (Fig. 4). According to the Ramachandran plot of
this model the f and c angles of most ofthe residues ... sequence of the
protein is underlined. The arrowheads indicate the
positions ofthe intron sequences. (B) Comparison
of the amino-acid sequences of b-amylase from
C. sepium (this work) with those from...