progression what are we learning from clinical experience and where are we going
Báo cáo khoa học: cAMP increases mitochondrial cholesterol transport through the induction of arachidonic acid release inside this organelle in Leydig cells pdf
... cycloheximide (CHX) and Waymouth MB752 ⁄ cell culture media were purchased from Sigma Chemical Co (St Louis, MO, USA) Nordihydroguaiaretic acid (NDGA) and AA-CoA were from Fluka (Buchs, Switzerland) Sera, ... washing the cells, they were scraped and nuclear and mitochondrial fractions were obtained as described in the Experimental procedures The fractions were sonicated and lipids were extracted with ... sucrose, mm EDTA, 10 mm KCl and 1.5 mm MgCl2 containing 500 ng of unlabeled AA, and were then sonicated Protein concentration was measured and lipids were extracted from equal amounts of nuclear...
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Salts Transport in Alkali Soil Reclamation by Gypsum and Prediction of Na Leaching in Field in China
... emissions from fossil fuel combustion are estimated to lie between 2,050 and 2,445 million tons of CO2 per year CO2 emissions from industrial processes are estimated to lie between 81 and 104 million ... application rates of 0.5 wt% and 1.0 wt% Alkali soil and gypsum were mixed uniformly and were packed in an acrylic ring with an outer diameter of 60 mm, inner diameter of 50 mm, and height of 20 mm at ... rate were higher than those at the 0.5 wt% application rate From Fig 3, it could be confirmed that the hydraulic conductivity (K1-4) between TD and TD increased rapidly and then decreased, from...
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Spatial moment analysis of colloid facilitated radionuclide transport in a coupled fracture-matrix system
... radionuclides and colloids are presented in Table and The base case data pertaining to radionuclides is adopted from from Li et al [19] and colloids from Abdel-Salam and Chrysikopolous [32] Table Parameters ... sorption between a radionuclide and both mobile and filtered colloids In this work, the adsorption behavior was represented by equilibrium distribution coefficients and sensitivity studies were performed ... J., and Gera, F Nuclear waste disposal: Understanding what happens underground IAEA BULLETIN 1985 35-40 [2] Wickham, S.M., Bennett,D.G., and Higgo,J.J.W Evaluation of colloid transport issues and...
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Tài liệu Women, Development and Transport in Rural Eastern Cape, South Africa ppt
... situation: [M]any of us know that we are so sick when we start walking to the clinic and we are lucky if we are going to come back alive we should get help when we first get sick but it is not ... discipline in South Africa and although areas of interest include gender and health, gender and education, gender and public policy, gender and masculinity, and gender and leadership, the issues ... to and from the river, minutes of travelling for primary production purposes, and 52 minutes of travelling to and from the field In addition to this, 74 minutes are spent travelling to and from...
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Tài liệu Personal Exposure to Air Pollution for Various Modes of Transport in Auckland, New Zealand doc
... emissions are its major source and reliable portable technology exists for its measurement Average concentrations and commute times are compared between modes and the impact of activity level and travel ... one from the east (Glen Innes) and one from the west (Waterview) Fig (1) shows Auckland’s coastline, major roads and the routes chosen by the commuters for the study, constrained by the start and ... Auckland Region and the routes chosen for the study Note that while the start and end points were specified, the commuters were free to choose the specific route total, data from 88 commutes were...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx
... equation and the apparent KM and Vmax values were derived with their standard errors The competitive and noncompetitive Ki values were derived from the equations KMi ¼ KM (1 + [I] ⁄ Ki) and ⁄ VmaxI ... as a standard Reagents Anthocyanins were from Polyphenols Laboratories (Sandnes, Norway), biliverdin from Frontier Scientific Europe Ltd (Carnforth, UK) All other chemicals were purchased from Sigma-Aldrich ... purchased from Sigma-Aldrich and Carlo Erba (Milan, Italy), and were of the highest available grade Acknowledgements Thanks are due to Prof G.L Sottocasa and Dr Antonella Bandiera (University of Trieste)...
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Tài liệu Báo cáo khoa học: Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity docx
... 32-0432 were from Calbio¨ chem LY-294002, microcystin LR and wortmannin were obtained from Alexis Myristoylated pseudosubstrate peptide inhibitors for PKC-a/-b and PKC-k/-f were purchased from Biomol ... (C-17) and GLUT4 (C-20) were obtained from Santa Cruz Biotechnology, Inc Rabbit polyclonal antibody recognizing IRb-chain and mouse monoclonal integrin b1, Cbl and PKC-k were purchased from Transduction ... 3T3-L1 adipocytes were stimulated with agonists and immunoprecipitated using time, concentration and antibodies as indicated in the figure legends Cells were lysed and IRS-1 and p85 immunoprecipitates...
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Báo cáo khoa học: Protein transport in organelles: The Toc complex way of preprotein import pdf
... complex [12] Two additional components, Toc64 [13] and Toc12 [14], were identified later, and are implicated in preprotein targeting to the Toc complex and heat shock protein (Hsp) 70 recruitment to ... (At5g05000) AtToc120 and atToc132 are highly redundant and may be more selective for nonphotosynthetic, housekeeping preproteins However, mutant analyses not exclude a specificity overlap between atToc159 ... of regulation are on the ‘most wanted’ list of the chloroplast import research community (Fig 3): the list includes kinases and phosphates as well as co-GAPs and GDP ⁄ GTP GEFs We expect that...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... and some key functional domains are additionally indicated (Tic40 and Tic62); Toc components are not labelled The predicted transmembrane domains of Tic40 and Tic55 are shown as small columns protruding ... at Tic32 and are integrated at the Tic complex Conclusions Increasing evidence is accumulating to suggest that we experience not only the one Tic complex, but also that the composition and activity ... with channel function (e.g in ligand-gated and voltage-gated K+ channels) [16], and thus could provide an explanation for the observed channel activity of Tic110, as well as for the binding of transit...
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Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx
... recognition and interaction events between the LHCP substrate and the cpSRP subunits Binding between LHCP and cpSRP43 is mediated by a conserved 18 amino acid span, termed L18, positioned between TM2 and ... structures are four sequential ankyrin repeats that are located between CD1 and CD2 ⁄ CD3 [51] These ankyrin repeats (ANK 1–4) have been implicated in protein–protein interactions and are likely ... Aldridge et al case is formed; and (iii), the precursor is transported and released from the translocase into the lipid bilayer where the signal peptide is removed and the mature protein is released...
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Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt
... proteins to chloroplasts and the nucleus [7], to chloroplasts and the peroxisome [8,9], and, in Chlamydomonas reinhardtii, to chloroplasts and the endoplasmic reticulum [10] However, by far the greatest ... that they are rather similar to plastidic and mitochondrial targeting signals, in that they are enriched in positively charged residues, and significantly deficient in acidic residues and glycine ... glycine [19] However, there are no features detectable to date that could distinguish them as a group from location-specific targeted proteins They appear to fall between mitochondrial and chloroplastic...
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Event-to-Sink Reliable Transport in Wireless Sensor Networks docx
... also that we assume that as long as sensor nodes are within the coverage area and hence have readings of the event features, they packetize their readings and send them to the sink AKAN AND AKYILDIZ: ... radio range and IFQ (buffer) length were carefully chosen to mirror typical sensor mote values [5] One of these nodes was chosen as the sink to which all source were randomly chosen data were sent ... decision intervals and the state transitions; and only the values of and varied very slightly Therefore, we show one graph for each initial state The corresponding trace values and states are listed...
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STOCHASTIC DYNAMICS Modeling Solute Transport in Porous Media pptx
... dynamics We hope that the engineering and science students and researchers would be interested in this promising area of mathematics as well as in the problems we try to discuss here We explain ... become highly random and modeling that component using a Fickian model is not desirable We are left with the option of modeling velocity as a random variable, and the degree of randomness depends ... differentiable at any point Without going into details of how we computed this function- we will that in Chapter - we can see that the increments are irregular and we can not define a derivative according...
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Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx
... pellet and the supernatant were analyzed by SDS ⁄ PAGE and immunoblotting a-MGD1, a-Tic22, a-Tic110 and a-Toc75 were used for immunodecoration whereas the marker proteins Toc75 and Tic110 were ... [35S]pMGD1 and [35S]pSSU, [35S]pTic22 and [35S]pSSU and [35S]pTic22 and [35S]pMGD1 in the same reaction were performed After import, chloroplasts were reisolated on a Percoll cushion and all samples were ... ampicilin (and mm MgSO4 and 0.4% glucose for mOE33) to an D600 nm of 0.6 Expression was induced by mm isopropyl thio-bd-galactoside and cells were grown for h at 37 °C pOE33 and mOE33 were purified from...
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Báo cáo khoa học: cGMP and glutathione-conjugate transport in human erythrocytes The roles of the multidrug resistance-associated proteins, MRP1, MRP4 and MRP5 pot
... MRP4 and MRP5 in human erythrocytes and COR-L23 cells Inside-out membrane vesicles prepared from human erythrocytes, COR-L23/P and COR-L23/R cells, and crude lysates from MOR/ADR cells were size ... squares fits were obtained for specified values of n2 (Table 2) Acceptable fits (likelihood ratio ¼ 0.05 compared to the best fit) were obtained for values of n2 between and 1.48 Over the range from ... Tween 20, verapamil and vincristine were all obtained from Sigma Chemicals Calcein was purchased from Molecular Probes Staurosporine and bisindolylmaleimide IX methanesulfonate (Ro 31–8220) were...
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cross plane thermoelectric transport in p type la0.67sr0.33mno3 lamno3 oxide metal semiconductor superlattices
... Adam, and B Wees, “Thermally driven spin injection from a ferromagnet into a non-magnetic metal,” Nat Phys 6, 879 (2010) 33 F Bakker, A Slachter, J Adam, and B Wees, “Interplay of Peltier and Seebeck ... quality Highly aligned grains and in-plane epitaxy were confirmed by asymmetric 110 phi scans of LSMO on STO, which showed that all four 90 -separated film peaks were well-aligned with the substrate ... http://jap.aip.org/about/rights _and_ permissions 193702-4 Jha et al scans of superlattices, which showed that all four 90 separated film peaks of LSMO and LMO were well-aligned with the STO substrate...
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transport in multilayered nanostructures. the dynamical mean-field theory approach, 2006, p.343
... different bands When we construct the ground state, we find that for band metals, many of the bands are either totally filled or totally empty, and only a small number of the bands are partially ... If we consider a collection of N nuclei, whose momenta, position, and mass are denoted by capital letters (Pj, Rj, and Mi), and a collection of N' electrons, whose momenta, position, and mass are ... If we are interested in the lowenergy properties, we can restrict ourselves to consider those partially filled bands only The band structure can be parameterized by a tight-binding scheme, where...
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báo cáo hóa học:" Caveolin-1 enhances resveratrol-mediated cytotoxicity and transport in a hepatocellular carcinoma model" docx
... and caspase-3 protein levels were further determined by Western blot (B) – HepG2 variants were treated with 200 μM RES for 24 h; CAV-1, caspase-3 and MAPKs protein levels were determined by Western ... acid, t-FA), Diethylstilbestrol(DES), Tamoxifen citrate and all other reagents used for immunofluorescence and Western blots were from Sigma and of the highest grade available Plasmids The mammalian ... animal welfare regulations under an approved protocol by the Beijing Bureau of Science Animal 40 Balb/c-nu female nude mice weighing 17–20 g were randomly assigned to groups Xenografts were established...
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