principles of 1 h nmr spectroscopy in vivo
Báo cáo khoa học: Structural function of C-terminal amidation of endomorphin Conformational comparison ofl-selective endomorphin-2 with its C-terminal free acid, studied by 1 H-NMR spectroscopy, molecular calculation, and X-ray crystallography pot
... 2 .19 1. 93 2. 01 2 .16 1. 87 1. 90 15 4 15 5 15 4 15 7 14 6 17 4 16 5 15 8 14 8 13 9 13 9 16 7 14 8 16 7 17 3 17 2 16 2 13 8 14 5 16 4 15 6 14 6 17 0 16 4 x,y,z x +1, y,z x +1, y,z 1- x,y +1 ⁄ 2,-z x,y,z x,y,z x,y,z x,y,z x -1, y,z ... Phe4 NH Phe4 NH Phe4 NH Phe4 NH Medium Weak Weak Medium Medium Tyr1 2, 6H Pro2 b1 Phe3 b2 Phe3 2, 6H Phe3 2, 6H Phe3 3, 5H Phe3 3, 5H Phe3 Phe3 Phe4 Phe4 Phe4 Phe4 Phe4 a 2, 6H 2, 6H 2, 6H 3, 5H a 2, 6H ... 2, 6H Phe3 3, 5H Phe3 3, 5H Phe3 Phe4 Phe4 Phe4 Phe4 Phe4 a a 3, 5H 2, 6H a 2, 6H Weak Weak Medium Medium Medium Medium trans EM2OH Pro2 b1 Pro2 c1 Phe3 b1 Phe3 b2 Phe3 Phe3 Phe4 Phe4 NH 2, 6H 2, 6H NH...
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Báo cáo khoa học: Characterization of oligosaccharides from the chondroitin/dermatan sulfates 1 H-NMR and 13 C-NMR studies of reduced trisaccharides and hexasaccharides doc
... H2 H3 H4 H1 H2 H3 H4 H5 H6 H6 ¢ NAc H1 H2 H3 H4 H5 H1 H2 H3 H4 H5 H6 H6 ¢ NAc H1 H2 H3 H4 H5 H1 H1 ¢ H2 H3 H4 H5 H6 H6 ¢ NAc 5 .19 3* 3.796* 4 .10 8* 5.885* 4.593 4.027 3.949 4 .17 6 3.996 4. 218 4.242 ... (ring C) signals fall within 0.02 p.p.m of Proton CS#604 (p.p.m.) CS#606 (p.p.m.) GalNAc (C) H1 H2 H3 H4 H5 H6 H6 ¢ CH3 H1 H2 H3 H4 H5 H1 H1 ¢ H2 H3 H4 H5 H6 H6 ¢ CH3 4.558* 3. 917 * 3.743 3.988* 3.967* ... C4 C5 10 2.83 71. 54 67. 51 109. 41 147. 01 – 10 2.97 55.02 78.24 79.04 77. 41 63. 91 25.27 17 7.79 65. 61 74.58 73.04 82.89 76 .11 10 4.25 72.60 68.89 11 0 .11 14 7.62 17 2. 21 103.70 54 .12 82.50 70. 51 75.60...
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Báo cáo khoa học: Detergent-resistant membrane fractions contribute to the total 1 H NMR-visible lipid signal in cell potx
... contained higher levels of 18 :0, 18 :2 + 18 :3 and 18 :1 at the expense of 16 :0, 14 :0 and 16 :1, whereas there was a marked increase in the amount of 16 :0 and a decrease in the amount of 16 :1 and 17 :1 ... however, neither the choline headgroups of PtdCho and sphingomyelin (except for a small peak in THP -1) nor the sphingosine chain of sphingomyelin were visible in the NMR spectra of DRMs In bilayer environments, ... with
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Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx
... T cells* C14 >10 >10 >10 HIV-1Ba-L 1. 3 ± 01 HIV-1NDK 0.02 ± 0.0 HIV-1Ba-L 1. 3 ± 0 .1 HIV-1NDK 1. 8 ± 0 .1 HIV-1Ba-L 0.8 ± 0.0 HIV-1NDK 0.7 ± 0 .1 Enfuviritid (T20) >10 >10 >10 0.08 ± 0 .1 ± 0.5 0.3 ... infection of macrophages, DC and T cells Therefore, HIV -1 sensitive cells were incubated with R5-HIV-1Ba-L or X4-HIV-1NDK in the presence of increasing non-toxic doses of C14 (Table 1) C14 inhibited ... STDs in south-western Uganda Cult Health Sex 2000, 2(2) :19 7- 211 Lee-Huang S, Huang PL, Huang PL, Bourinbaiar AS, Chen HC, Kung HF: Inhibition of the integrase of human immunodeficiency virus (HIV)...
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Báo cáo khoa học: Calcium-independent phospholipase A2-mediated formation of 1,2-diarachidonoyl-glycerophosphoinositol in monocytes potx
... (19 86) Remodeling of arachidonate-containing phosphoglycerides within the human neutrophil J Biol Chem 2 61, 77 71 7777 Fonteh AN & Chilton FH (19 92) Rapid remodeling of arachidonate from phosphatidylcholine ... of 1, 2-diarachidonoylglycerophosphocholine in human neutrophils Biochem Biophys Res Commun 14 5, 11 26– 11 33 Kuwae T, Schmid PC & Schmid HH (19 96) Alterations of fatty acyl turnover in macrophage ... P388D1 macrophage-like cells, an event that paralleled the inhibition of AA incorporation into phospholipids [10 ,11 ,30–32] Given the above data showing that BEL blocks [ 2H] AA incorporation into...
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báo cáo hóa học: " The fixed point theorems of 1-set-contractive operators in Banach space" docx
... http://www.fixedpointtheoryandapplications.com/content/2 011 /1/ 15 Page of Remark 2 .16 Corollary 2 .15 extends Theorem of Li [6] Putting b = in Corollary 2 .15 , we can obtain Theorem of Li [6] Theorem 2 .17 Let E, Ω, A be the same as in Lemma 1. 1 Moreover, ... fixed point theorems for 1- set-contractive operators in Banach spaces Nonlinear Anal 67, 938–944 (2007) doi :10 .10 16/j.na.2006.06.0 51 doi :10 .11 86 /16 87 -18 12-2 011 -15 Cite this article as: Wang: The fixed ... Fixed Point Theory and Applications 2 011 , 2 011 :15 http://www.fixedpointtheoryandapplications.com/content/2 011 /1/ 15 Page of Suppose this is not true Then there exists x0 Î ∂Ω, t0 ≥ such that Ax0...
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Báo cáo khoa học: "The of near-infrared reflectance spectroscopy in litter decomposition studies" doc
... reflectance in the 11 00 -14 00 region caused by to a the increase of mineral component agrees with data from Paul (19 88) for soil contamination in silage and Windham et al (19 91) for increasing ash concentration ... results obtained with and without the detrending method (Bames et al, 19 89) were compared Stepwise is performed by selecting the wavelength that is the most highly correlated with the reference ... adding it to the equation The second wavelength is added by calculating all partial correlations with all other wavelengths and selecting the wavelength with the highest partial correlation The...
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báo cáo khoa học: " Intein-mediated site-specific conjugation of Quantum Dots to proteins in vivo" pdf
... microinjection together with the IC-QD's In vivo association of the intein halves in the cytosol triggered protein trans-splicing, resulting in the ligation of the QD to the target protein through a peptide ... confirmation of the conjugation in the intact embryo Briefly, we genetically tagged EGFP fusions of the PH domains of Akt and Btk with the N-terminus half of a split intein (IN) The complementary C-terminus ... with RNA encoding the target protein (in this case, Akt PH-EGFP -IN) The presence of EGFP on the PH domain allowed us to monitor and compare the distribution of the QD's vs the Akt-PH As shown in...
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Structural and equilibrium unfolding studies of sam domain of DLC1 by NMR spectroscopy
... spectroscopy 1. 2 .1. 1 NMR phenomenon 1. 2 .1. 2 Basic NMR parameters 1. 2 .1. 2 .1 Chemical shift 1. 2 .1. 2.2 J-coupling 1. 2 .1. 2.3 Nuclear Overhauser Effect (NOE) 1. 2 .1. 2.4 Chemical exchange 1. 2 .1. 2.5 Relaxation 1. 2.2 ... ABBREVIATIONS viii 11 1 11 1 11 6 12 0 12 7 13 1 14 1 14 7 14 9 15 1 15 3 16 7 LIST OF TABLES Table 3 .1 Table 3.2 Table 3.3 Experimental restraints and structural statistics for 10 lowest-energy NMR structures of SAM76 ... denaturant 1. 3.2.3 Three main spectroscopic techniques used for protein folding studies 1. 3.2.3 .1 Circular dichroism (CD) spectroscopy vi i iii iv ix x 1 5 10 10 11 12 12 13 14 14 16 17 18 18 20 21 23...
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Photoimmunotherapy of melanoma via combination of hypericin photodynamic therapy and in vivo stimulation of dendritic cells by PNGVL3 HFLEX plasmid DNA
... merocyanine C540 and photofrin II in achieving tumor control in vitro They attributed this finding to the mechanism of tumor destruction of Verteporfin which strongly depended on the high yield of singlet ... (meta-tetrahydroxyphenylchlorin) and Photofrin (Hematoporphyrin derivative) The most commonly used photosensitizer 11 presently is probably Photofrin However, this first generation photosenstizer has ... with the findings demonstrated by 29 Leunig et al., 19 94 in their study evaluating the effect of PDT-induced heating of melanoma in vivo Subsequently, several others have confirmed that PDT using...
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Báo cáo y học: "Expression of Human Globular Adiponectin-Glucagon-Like Peptide-1 Analog Fusion Protein and Its Assay of Glucose-Lowering Effect In Vivo"
... of China (No: 306 710 07, 3030 016 5) and the grant from the Traditional Chinese Medicine Administration of Zhejiang Province, China (No: 2 010 ZB075) 209 10 11 12 13 14 15 16 17 Chen J, Bai G, Cao ... 5, 14 ] Thus, http://www.medsci.org Int J Med Sci 2 011 , we connected the N-terminus of globular adiponectin and the C- terminus of GLP -1- A through the linker, which could make the N-terminus of ... glycine-rich linker is flexible, which allows the specific engineering of hinge regions into proteins to achieve desired functional motions [17 ] We used a glycine-rich short peptide including 15 amino...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... Fig 1H ,15 N-HSQC spectra of the M domain of human eRF1 The numbering of the residues corresponds to that of the full eRF1 protein (A) The Gly region of the 15 H, N-HSQC spectrum of the M domain of ... in the family of 25 NMR structures of the M domain of human eRF1 Ranges of torsion angles in whole familya Residue / Pro177 Lys178 Lys179 His180 Gly1 81 Arg182 Gly183 Gly184 Gln185 Ser186 Ala187 ... The C domain of eRF1 interacts with the C domain of eRF3 [15 18 ], and the binding of both factors is essential for fast kinetics of the termination of translation [1] However, in a simplified in...
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Báo cáo khoa học: Epitope mapping of the O-chain polysaccharide of Legionella pneumophila serogroup 1 lipopolysaccharide by saturation-transfer-difference NMR spectroscopy pot
... dependence of the N-methyl signals long-chain PSNH4OH/HF from wild-type RC1 was dissolved in 10 % deuterated water, the pH was adjusted within a range of pH to pH 11 with M HCl or 1 M NaOH and recording ... and short-chain PSNH4OH/HF from wildtype RC1 were investigated by 1D 1H NMR spectroscopy and signal integration was performed to calculate the average chain-length of the PSNH4OH/HF and the distribution ... separated in the corresponding 1D 1H NMR spectra Therefore, participation in binding of these residues located at the reducing end of PSNH4OH/HF could not be con®rmed by our experiments The 1D 1H NMR...
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Báo cáo Y học: In vivo activation of plasma membrane H+-ATPase hydrolytic activity by complex lipid-bound unsaturated fatty acids in Ustilago maydis docx
... and linoleic acid-containing phosphatidylcholine was added (82.9 ± 8.9% of the control) Conversely, when 1- palmytoyl-2-linoleyl-phosphatidylcholine was added to these vesicles, an increase in ATPase ... [33] In U maydis, changes in the plasma membrane 18 : 2 /18 : ratio followed the inhibition of growth rate, which was in uenced not only by the biosynthetic point affected but also by the method ... reports, the joint use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the in uence of direct effects, i.e sterol alteration, and indirect...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
... 6-phosphofructo -1- kinase and inhibit fructose -1, 6-bisphosphatase, which should have reduced the activity of the indirect pathway of glycogen synthesis, as it depends on flux through fructose -1, 6-bisphosphatase ... fructose -1, 6-bisphosphatase However, it is likely that the increased rate of 13 C label incorporation into the C6 position of glycogen in the ADM group is indicative of activated 6-phosphofructo -1- kinase, which ... clarified the in vivo action of Fru-2,6P2 on the 6-phosphofructo -1- kinase/fructose -1, 6-bisphosphatase cycle, suggesting that the activation of glycolysis predominates over the inhibition of gluconeogensis...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... O-chain polysaccharide 204.04 217 .03 218 . 01 3 91. 11 404 .11 405.08 649 .17 662 .11 663 .16 12 97.37 13 10.34 13 24.23 13 25 .18 203.08 216 .08 217 .06 390 .17 403 .17 404 .15 648.29 6 61. 29 662.27 12 96.58 13 09.58 ... used in most of 1D analogues of 2D NMR experiments The 1D 1H- NMR spectrum of CPS showed three resonances in the low-field region (5.3–4.4 p.p.m.) in the relative Ó FEBS 2004 ratio of : : 3, of which ... structure of the CPS and O-chain polysaccharide of A salmonicida strain 80204 -1 the previously identified O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11 ], confirming that the...
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Báo cáo khoa học: Solution structure of long neurotoxin NTX-1 from the venom of Naja naja oxiana by 2D-NMR spectroscopy pot
... modification of cationic residues in toxin a from King cobra (Ophiophagus hannah) venom J Protein Chem 15 , 95 10 1 Grishin, E.V., Sukhikh, A.P., Slobodyan, L.N., Ovchinnikov, Y.A & Sorokin, V.M (19 74) Amino ... venom: a proton NMR and molecular modeling study Biochemistry 31, 11 335 11 347 Ruan, K .H. , Stiles, B.G & Atassi, M.Z (19 91) The short neurotoxin binding regions on the a-chain of human and Torpedo ... Saenger, W (19 91) The refined crystal structure of a-cobratoxin 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 ˚ from Naja naja siamensis at the 2.4 A resolution J Biol Chem 266, 215 30– 215 36 Dewan,...
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Báo cáo khoa học: Hodgkin Reed–Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo Novel insight into the inflammatory features of classical Hodgkin lymphoma ppt
... with marked high eosinophilia However, there were also cases with high eosinophilia and weak staining of 15 -LO -1 in H- RS cells (Table 1) By contrast, no staining of 15 -LO -1 was observed in tumour ... medium eosinophilia (50 12 0 eosinophils per 10 HPF) or high eosinophilia (> 12 0 eosinophils per 10 HPF) The number of H- RS cells were classified as few (< H- RS cells per 10 HPFs), medium (5 10 H- RS ... The biopsies were then classified as low eosinophilia (< 50 eosinophils per ten HPF), medium eosinophilia (50 12 0 eosinophils per 10 HPF) or high eosinophilia (> 12 0 eosinophils per ten HPF) The...
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Báo cáo Y học: Expression of the uncoupling protein 1 from the aP2 gene promoter stimulates mitochondrial biogenesis in unilocular adipocytes in vivo potx
... explain the lack of the effect of the transgene on the size of the latter depot [16 ,17 ]; this is also associated with the differential effect of the transgene on in situ fatty acid synthesis in the ... D (19 94) In vitro interactions between nuclear proteins and uncoupling protein gene Ó FEBS 2002 10 11 12 13 14 15 16 17 18 19 20 21 promoter reveal several putative transactivating factors including ... during ageing in either genotypes However, as indicated by ANOVA, there was a main effect of genotype in both depots, with transgenic animals showing higher levels of the transcripts Within different...
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