primary neurons in culture and neuronal cell lines for in vitro neurotoxicological studies
Báo cáo y học: "Data from necropsy studies and in vitro tissue studies lead to a model for allometric scaling of basal metabolic rate" pot
Ngày tải lên :
13/08/2014, 23:20
... defined confidence interval for the scaling exponent Note that no information on metabolic rates in the studies of Heusner and Kleiber is used in generating the BMR predictions Discussion In ... midpoint of the values of ki plus the midpoint of the values of bi because values of ki are not available for certain tissues For scaling of the brain and the epithelial tissues of the skin and ... 0.56 and 0.91, respectively, and the MLE for α1/α2 is 0.57/0.43 The MLE estimates for β1 and β2 are close to the exponents in Equation (4) estimated from data gathered by necropsy and in vitro studies...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
Ngày tải lên :
18/06/2014, 19:20
... b-actin The PCR conditions were as follows: for c-IAP-1 and XIAP, 94°C for min, 62°C for min, and 72°C for min; for Bcl-2, 94°C for 30 s, 62°C for 30 s, 72°C for 10 s; and for b-actin: 94°C for ... of curcumin in DNR-insensitive CD34+ AML cell lines (KG1a and Kasumi-1) and in CD34+ primary AML samples We showed that curcumin selectively induced apoptosis in KG1a and Kasumi1 cell lines, as ... participated in its design and coordination DX contributed effort in designing, performing, and analyzing the results and conclusion All authors read and approved the final manuscript Competing interests...
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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx
Ngày tải lên :
07/03/2014, 11:20
... possible induction of cell stress in the skin by WÆkg)1 GSM-900 exposure for 48 h No apoptosis was induced in either skin cell type, in agreement with reports of other in vitro studies concluding ... the skin or skin cells did not use the same endpoints For instance, in vitro exposure at 900 or 1800 MHz was found to affect gene expression and induce DNA damage in a fibroblastic cell line and ... GSM-900 and cell stress in skin models S Sanchez et al expression under stress conditions has been reported in a number of cell types, including skin cells The major HSPs expressed in the skin [5]...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
Ngày tải lên :
07/03/2014, 16:20
... cancer cell lines express Cdt1 over 10-fold more than primary cell lines Geminin is hardly detectable in the primary cell lines, and accumulates to higher levels in the majority of cancer cell lines ... over-expression of the geminin protein differs between cell lines, resulting in gross differences in relative amounts of Cdt1 and its inhibitor in different cell lines It would be interesting to investigate ... two genes in different tumor cell lines and compare them to primary cells Cellular lysates were prepared from human foreskin fibroblasts, a primary cell line, and the tumorigenic cell lines Saos,...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... the major cellular site for ROS formation, reduces enzymatic and soluble intracellular antioxidant defences but not ROS flux in the studied q0 cells, and that there are cell line-to -cell line variations ... the cells were incubated in NaCl/Pi for 90 min, the intracellular fluorescence signal dramatically increased in all cases (Fig 9, Table 1) The increases, in comparison to the signals observed in ... expression and protein content of SOD (Figs 3–5) Antioxidant defences of q0 cell lines GSH-GSSG and GR We measured GSH and GSSG in exponentially growing cells, as GSH content changes in the growth and...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
Ngày tải lên :
17/03/2014, 23:20
... HTLV-1 and is deficient in the signaling IL-2Rb and c subunits [25] All cell lines were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, penicillin and streptomycin [11] ... subunits and HLA glycoproteins in T leukemia and lymphoma cell lines (A) Representative FRET efficiency (E, %) histograms measured on T lymphoma/leukemia cell lines, on cell- by -cell basis, using flow ... and cc chains, is associated with cholesterol- and glycosphingolipid-rich membrane microdomains (rafts) in cell lines of human adult T cell lymphoma/leukemia origin, independently of the ligand...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
Ngày tải lên :
22/03/2014, 21:20
... primers for ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing, China) ... Logan, UT, USA); MDA-MB-231 cells were maintained in 10% fetal bovine serum-supplemented L-15 (Gibco, Grand Island, NY, USA); SK-BR-3 cells were maintained in 10% fetal bovine serum-supplemented RPMI-1640 ... vector) for 24 h incubation (C) Single -cell suspensions containing 20 000 cells treated respectively were seeded in each well of 24-well plates at 37 °C as attached monolayers in DMEM containing...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
Ngày tải lên :
23/03/2014, 07:20
... values were determined using linear regression analysis of the dose–response curves Cell culture and cytotoxicity assay HaCaT cells, HEL cells and HEp-2 cells were cultured in DMEM supplemented ... selenoureas and selenoamides using three cell lines, HaCaT, HEL, and HEp-2, and PMNs, by the microtiter tetrazolium method [41–43] The cytotoxicities of the compounds in each cell line are shown in Fig ... compounds in some human cell lines and PMNs Cytotoxicity was determined by microtiter tetrazolium assay ‘Control’ contained cells plus cell culture medium supplemented with 2% fetal bovine serum...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... of human cell lines; primate and canine cell lines may also be used [5] It has been extensively documented that the plasmids are maintained episomally (5–10 copies per cell, e.g for 293 cells), ... plasmid pEBTetD for full establishment of doxycycline-inducible protein expression in human cell lines by just a single transfection For closely matching cellular backgrounds we use continuous expression ... our results for pEBTetD cell pools in continuous culture up to 10 weeks indicate useful factors of inducibility on the level of protein function Clearly, in many situations, e.g in RNA interference...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
Ngày tải lên :
18/06/2014, 15:20
... wound tissue in the OR and HE helped in logging de-identified clinical data and delivering the specimens to MTC MTC supervised OS and SV to carry out the culture the cells in- vitro and perform the ... "response margin" can be established in a wound Biopsies of tissue and their subsequent cell cultures would define this response margin and indicate further debridement For the surgeon, findings presented ... mycoplasma testing by PCR In Vitro Cell Dev Biol Anim 1998, 34:356-358 McGarrity GJ, Coriell LL: Detection of anaerobic mycoplasmas in cell cultures In Vitro 1973, 9:17-18 Fibroblast Cell Lines [http://ccr.coriell.org/Sections/Search/...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
Ngày tải lên :
18/06/2014, 19:20
... stimulating the growth of one cell line in our panel, and the antagonistic effects of the combination of metformin and vemurafenib in several cell lines, the combination of vemurafenib and metformin ... melanoma cells to each drug alone, both drugs were combined in constant ratios to each other Among the 11 BRAFV600E mutant cell lines, in cell lines the combination was antagonistic and in cell lines ... μM vemurafenib and 10 mM metformin of all melanoma cell lines tested The green cell lines harbor BRAFV600E, red shows NRASQ61 mutant cell lines, and black the wild type cell line M257 Propidium...
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Báo cáo sinh học: " High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" potx
Ngày tải lên :
18/06/2014, 22:20
... hematological cell lines using cell cycle analysis and cell death measures to determine sensitivity and resistance Cell lines that are early and moderate responders by cell cycle analysis with a Ymin/T0 ... lymphoma cell lines, of the cell lines were resistant to GSK1070916 and contained low chromosome number in the primary population of cells However, when in reviewing the ploidy content in the cell ... quantified Defining Cell Sensitivity An analysis of cell line sensitivity to GSK1070916 was performed with the data generated from screening cell lines in cellular proliferation assays and from cell cycle...
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Báo cáo hóa học: " Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray" docx
Ngày tải lên :
20/06/2014, 01:20
... transform human B cells in vitro [2], and EBV-harboring cell lines constitute a key research tool to study pathogenic events leading to lymphocyte transformation and oncogenesis As noted in studies ... Furthermore, in Namalwa cells, the EBV genome is integrated in the human chromosome [34], while the other BL cell lines harbor the virus in episomal form Finally, although BL cells in culture initially ... quantified during induction of lytic EBV infection in Akata cells by BCR cross-linking For each time point, treated and non-treated cells were harvested and subjected to qPCR Each point represents...
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o cáo hóa học:" High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" ppt
Ngày tải lên :
20/06/2014, 04:20
... hematological cell lines using cell cycle analysis and cell death measures to determine sensitivity and resistance Cell lines that are early and moderate responders by cell cycle analysis with a Ymin/T0 ... lymphoma cell lines, of the cell lines were resistant to GSK1070916 and contained low chromosome number in the primary population of cells However, when in reviewing the ploidy content in the cell ... quantified Defining Cell Sensitivity An analysis of cell line sensitivity to GSK1070916 was performed with the data generated from screening cell lines in cellular proliferation assays and from cell cycle...
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báo cáo hóa học:" Aberrant STYK1 expression in ovarian cancer tissues and cell lines" pdf
Ngày tải lên :
20/06/2014, 07:20
... 2receptors lines express STYK1 and variably express Ovarian tumor cell lines express STYK1 and variably express estrogen receptors HS832 is a benign ovarian cell line and the remaining cell lines were ... expression in ovarian cancer cell lines SKOV3, CaOv3, and OvCar3 (Fig 2A) Cell lines HS832, OvCar5, OvCar8 and IGROV had no detectable ER transcript While ER expression was weak for most of the cell lines, ... proliferated in media without serum or growth factors Inoculation of these cells into nude mice induced tumor formation within one week and the cells metastasized after weeks Introducing a tyrosine to...
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Báo cáo khoa học: "The combination effect of sodium butyrate and 5-Aza-2''''-deoxycytidine on radiosensitivity in RKO colorectal cancer and MCF-7 breast cancer cell lines" pot
Ngày tải lên :
09/08/2014, 04:21
... combined on radiosensitivity Materials and methods Cell line culture and reagents Human colon cancer cell lines RKO (ATCC, USA), breast cancer cell line MCF-7 (KCLB, Korea), and normal colon cell ... Diego, California, USA) combined with 10% fetal bovine serum and 1% penicillin/streptomycin using a humidified cultivator that maintained 37°C and 5% CO2 The normal cell line was cultivated using the ... 5-aza-DC and SB enhanced radiosensitivity in MCF-7 and RKO cell lines In RKO cell lines, which are in a relatively hypermethylated state, the effect of 5-aza-DC was similar to that of SB; in MCF-7 cell...
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Báo cáo khoa học: " Loratadine dysregulates cell cycle progression and enhances the effect of radiation in human tumor cell lines" pot
Ngày tải lên :
09/08/2014, 10:20
... grown in DMEM, and all other cell lines were grown in RPMI 1640 All media contained 10% heat-inactivated fetal bovine serum and antibiotics For cell survival studies, cells were plated (5 × 105 cells/ ... (3:1) and stained with crystal violet Colonies with >50 cells were scored and cell survival determined after correcting for the plating efficiency and for loratadine cytotoxicity alone For radiation ... transferred to nitrocellulose membrane using iBlot Dry Blotting System from Invitrogen (Carlsbad, CA) Non specific protein binding was blocked by incubating the membranes for hour in 3% blocking grade...
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Báo cáo khoa học: " The membrane targeted apoptosis modulators erucylphosphocholine and erucylphosphohomocholine increase the radiation response of human glioblastoma cell lines in vitro" pdf
Ngày tải lên :
09/08/2014, 10:20
... http://www.ro-journal.com/content/1/1/6 Cell lines, cell culture and cellular treatment T98G, U87MG and A172 astrocytoma/glioblastoma cell lines were from ATCC (Bethesda, Maryland, USA) For all experiments cells were grown in RPMI ... time- and concentrationdependent cell death in all cell lines with T98G and A172 cells being more sensitive than U87MG cells at all time points (Fig 1C–E) Human malignant glioma cell lines are ... AC/GBM cell lines to radiation induced cell death, in particular apoptosis To gain more insight into cytotoxic efficacy of ErPC/ErPC3 treatment alone and in combination with radiation, standard...
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