prevalence subtypes and gender differences
Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real-time PCR [8-11] For kinetic ... viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm we described previously...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... participated in design and coordination of the study and helped to draft the manuscript All authors read and approved the final manuscript Material and Methods Virus culture and virus detection ... http://www.virologyj.com/content/2/1/7 The respective MOI and time of cell culture are shown in table and were chosen to allow maximal infection as determined by immunofluorescence and real-time PCR [8-11] For kinetic ... viral RNA was performed to proof and monitor infection Thereafter the candidate reference genes were evaluated by the BestKeeper tool [6], the GeNorm tool [7] and the algorithm we described previously...
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Báo cáo y học: " Phosphodiesterase type 4 expression and anti-proliferative effects in human pulmonary artery smooth muscle cells" docx
... absence and presence of drugs at specified concentrations The medium was collected after 48 h and gelatinase (MMP-2 and MMP-9) activity visualized by zymography and measured using MMP-2 and MMP-9 ... lung and vasculature [11,12] PDE4 proteins are encoded by four genes (PDE4A, PDE4B, PDE4C and PDE4D), which produce numerous PDE4 variants [10,13] and studies on rat pulmonary arteries [14] and ... treatment of PASMCs with PMA (10-7 M) and TNF-α (10 ng/ml) and its inhibition by cicaprost and forskolin (A-B) Inhibitory effect of roflumilast, both alone (C) and in combination with a sub-maximal...
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Báo cáo y học: " Ancient, independent evolution and distinct molecular features of the novel human T-lymphotropic virus type 4" pot
... p30II, and p13II (where I and II denote ORFI and ORFII, respectively), have been identified in the pX region of HTLV-1 and are important in viral infectivity and replication, T-cell activation, and ... confidence intervals for the origin of HTLV-2 as it migrated out of Africa and Asia and into the Americas via the Bering land bridge and 40,000 – 60,000 ya as confidence intervals for the origin of HTLV-1 ... HTLV-2 and PTLV-3 sequences are drawn in red, blue and green respectively The branch leading to HTLV-4(1863LE), STLV-2, and to the divergent MarB43 strain are drawn in magenta, purple, and yellow...
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A comparative study of discourse structures and some major linguistic features of international declarations and international conventions on human rights part 4
... right to a standard of living adequate for the health and well-being of himself and of his family, including food, clothing, housing and medical care and necessary social services, and the right ... security and is entitled to realization, through national effort and international co-operation and in accordance with the organization and resources of each State, of the economic, social and cultural ... personality and to the strengthening of respect for human rights and fundamental freedoms It shall promote understanding, tolerance and friendship among all nations, racial or religious groups, and shall...
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Tài liệu E-Human Resource Management 4 pptx
... strategy and sharing of common values, communication and feedback processes, levels of trust and perceived organizational justice, employee commitment, and organizational readiness and flexibility ... have outlined above towards a new balance between innovation, trust, and learning on the one hand and new forms of discipline and control on the other will not be easy They will require a refocusing ... the financial and human resources required, the organizational structures, and the major systems and procedures These are the building blocks that can be redesigned, added to, and reconfigured...
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Báo cáo khoa học: The Saccharomyces cerevisiae orthologue of the human protein phosphatase 4 core regulatory subunit R2 confers resistance to the anticancer drug cisplatin pot
... supernatant and pellet fractions were analysed by SDS ⁄ PAGE and immunoblotting with MYC antibodies (A) YBL046w–MYC13 and HA3– Pph3p (B) YBL046w–MYC13 and HA3–Sit4p (C) YBL046w–MYC13 and HA3–Pph21p ... a null allele of YBL046w in AY925 and AY926 backgrounds and diploid strains homozygous for this allele in an AY927 background are viable and showed no growth differences from wild-type 3324 control ... nonessential gene and had overlapping properties with Pph21p and Pph22p, allowing limited growth in some genetic backgrounds when the PPH21 and PPH22 genes were deleted [3,4] In addition, Pph3p and the...
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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... °C and pH 7.5), there was no significant difference in the thermal stability of the wild-type and mutant enzymes; and (c) the ion exchange chromatographic properties on a DEAE–cellulose column and ... variant On the other hand, as a potential ligand for a primary amino group, Asp130 would contribute to the increased affinity of the mutant enzyme for agmatine and putrescine and, thus, to its ability ... that differences in this loop area are key factors in determining the differences in substrate specificity between arginase and agmatinase [31] Interestingly, when compared with rat liver and B...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... exclusion and actively translocate along a duplex J Biol Chem 278, 49171–49182 Bochman M & Schwacha A (2007) Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and ... MCM4/6/7 complex (120 ng) and SV40 T antigen (200 ng) in the presence of single-stranded DNA The radioactivity at the sites to which Pi and ATP migrated was measured, and the ratio of the released ... the MCM4/6/7 complex, SV40 T antigen and Werner protein, on the oligonucleotide displacement activity of replication protein A (RPA), and on the RNA priming and DNA polymerization activities of...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... L313M, G319E and R335H) are associated with peripheral forms of the disease In this study, all nine disease-causing mutations have been expressed in, and purified from, Escherichia coli, and their ... [27], and thus the observed activity probably reflects that of the native enzyme With the exception of L183P, all the mutant proteins could be expressed and purified using similar conditions and ... than threefold), whereas some mutants (especially G90E and V94M) showed large changes in kcat and in kcat ⁄ Km In contrast, two mutants (N34S and G319E) showed very little change in these parameters...
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Báo cáo sinh học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" pot
... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the ... in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI...
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Báo cáo sinh học: " The simultaneous presence and expression of human hepatitis C virus (HCV), human herpesvirus-6 (HHV-6), and human immunodeficiency virus-1 (HIV-1) in a single human T-cell" docx
... body and CIMM Authors' contributions K.A.S., A.G., and R.G performed biological work J.G.P and A.S.K performed the clinical work, recruitment of patients, and procurement of specimens K.A.S and ... fixation and processing Figure gels of PCR products Agarose3 Agarose gels of PCR products The DNA standard is a kb plus ladder (Invitrogen) Arrows indicate the expected band sizes Positive bands ... infections and other associated problems [4] Our effort is to understand the impact of multiple infections at a cellular level, both on virus reproduction and its characteristics and on their...
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Báo cáo sinh học: " Thiol-reactive reagents inhibits intracellular trafficking of human papillomavirus type 16 pseudovirions by binding to cysteine residues of major capsid protein L" pot
... the DE-loop and C225 and C229 are involved in forming the EF-loop Because the loops are generally flexible and because C146 and C225 are located at the surface region of the loops and C229 is ... (lanes and 8) and became resistant after the incubation at 37°C for h (lanes and 9) The cells inoculated with the 16PVs were further incubated with the growth medium at 37°C for 2, 4, 8, and 20 ... free thiols The DTNB-16PVs and the NEM-16PVs, whose C146, C225, and C229 carried DTNB and NEM as additional side chains, respectively, bound to HeLa cells less efficiently and were degraded rapidly...
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Báo cáo sinh học: " The E5 protein of the human papillomavirus type 16 down-regulates HLA-I surface expression in calnexin-expressing but not in calnexin-deficient c" pptx
... between the oncogenes HPV16 E5, E6 and E7 and different apoptotic pathways have already been identified [9] E6 and E7 modulate apoptosis by binding and inactivating p53 and the product of tumour suppressor ... CEM-C7 and CEM-NKR cell lines were electroporated using 1×107 cells in 200 µl PBS, 10 µg DNA and setting the pulser to 220 Volt and 960 µFarad (Bio-Rad GenePulser) Transfected CEM-C7 and CEM-NKR ... (N = 5) and the error bars comprise the corresponding standard deviations There were no differences between the total amounts of cellular HLA (N = 5; pKW = 0.87, Kruskal-Wallis test, and pA =...
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Báo cáo sinh học: " Human herpesvirus 6A induces apoptosis of primary human fetal astrocytes via both caspase-dependent and -independent pathways" potx
... and WXH designed and directed studies, and were involved in the interpretation of the data and writing of manuscript BG, GFZ and FZ carried out the cells culture and HHV-6 infection BG, JFW and ... assay and the western blot analysis BG, DJF and CZ performed the flow-cytometry experiments BG, JC and DDG performed the analysis of Caspase-8 and -9 activities and electron microscopy CLD and ... dehydrated in a series of 70%, 80% and 90% ethanol, and embedded in Epon Ultrathin sections were cut and mounted in nickel grids, stained with uranyl acetate and lead citrate, and examined by a transmission...
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Báo cáo sinh học: " Human herpesvirus 8 – A novel human pathogen" potx
... [34], in Uganda (38.7%) [34], 51%–62% [167]), and 55.5% in Cameroonian pregnant women [83] Similar HHV-8 prevalences were found for HIV+ persons in Uganda with between 45.7% and 71% HHV-8 prevalence ... particular isolate Data have shown that subtypes A and C are prevalent in Europe, the U.S.A., and northern Asia Subtypes B and A5 predominate in Africa and the D variant is found in the Pacific ... a prevalence of 80% [182] 6.A.d Europe In Europe, excluding Italy and its surrounding islands, the prevalence of HHV-8 in BDs was not above 6.5% in six countries: Hungary 0.83%–1.6%, Switzerland...
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nghiên cứu phát hiện sự gắn chèn gene e6 và e7 của human papilomavirus type 16 vào bộ gene người bằng kỹ thuật multiplex rt-pcr
... thi t k ánh giá m i Trong giai o n thi t k m i, qua tham kh o m t s tài li u v thi t k m i c a Andrew Wallace (1996), Alkami Biosystems (1999) , m t s tiêu chu n c a m i c rút sau: - Chi u dài...
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Nuclear Power Control, Reliability and Human Factors Part 4 docx
... Nuclear Power Systems 107 system-level information and commands, and spacecraft-level status and demands The effective integration of data and information at each level requires a well-defined ... (1999c) IEEE Standard 802.11a, Part 11: Wireless LAN Medium Access Control (MAC) and Physical Layer (PHY) Specifications High Speed Physical Layer in the GHz Band IEEE (2003) IEEE Standard 802.11g, ... (MAC) and Physical Layer (PHY) Specifications Further Higher Data Rate Extension in the 2.4 GHz Band IEEE (2005) IEEE Standard 802.11e, Part 11: Wireless LAN Medium Access Control (MAC) and Physical...
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Báo cáo hóa học: " Purification of infectious human herpesvirus 6A virions and association of host cell proteins" potx
... contributions MH and JA carried out the purification and analysis of the study with equal contribution and drafted the manuscript AF-H, SJ and HG participated in its design and coordination and helped ... shown Results are based on three experiments and presented as mean ± standard deviation (error bars) B and C Light microscopic analyses of HHV-6A- and mock-infected JJHAN cells at dpi Note the ... in HHV-6A infected cells D and E Detection of HHV-6 antigen gp60/110 in HHV-6A infected cells HHV-6A -and mockinfected cells were fixed and stained for gp60/110 (red) and counterstained with DAPI...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25