preparation of the substrate
Tài liệu EXPERIENCE OF THE INSTITUTE OF ENERGY IN THE PREPARATION OF THE RENEWABLE MASTER PLAN docx
Ngày tải lên :
24/01/2014, 03:20
... assessment.
Institute of Energy
RURAL RENEWABLE ENERGY WEEK IN HANOI
EXPERIENCE OF THE INSTITUTE
OF ENERGY IN THE
PREPARATION OF THE
RENEWABLE MASTER PLAN
Presented by: Nguyen Duc Cuong
Institute of Energy
Hanoi, ... MW
Geothermal energy and other types (tide, ocean waves )
¾ Potential of geothermal energy resources: 200- 340 MW.
¾ The renewable energy type such as tide and sea waves are
in the stage of potential ...
energy, and geothermal energy.
9 So far, even the high potential of RE resources, their contribution in electricity
production in Vietnam is still negligible.
9 The Government of VN recognized...
- 12
- 705
- 0
Tài liệu Báo cáo khoa học: An investigation of the substrate specificity of the xyloglucanase Cel74A from Hypocrea jecorina pdf
Ngày tải lên :
19/02/2014, 05:20
... sub-
contract from The Of ce of Biomass Program, within
the DOE Of ce of Energy Efficiency and Renewable
Energy. Tom Desmet holds a fellowship of the Insti-
tute for the Promotion of Innovation through ... presumably, contain the sites of O-glycosylation,
but we have not identified the specific site(s) or the
form of the O-glycans.
The CD spectrum of Cel74A looks a lot like that of
an unordered protein ... comparing the activity of Cel74A on 0.1 mm
XXXG, determined as described above, in the presence and
absence of 2 mm inhibitor. The inhibitors were not hydro-
lyzed in the time course of these experiments...
- 8
- 646
- 0
Tài liệu Báo cáo khoa học: Comparison of the substrate specificity of two potyvirus proteases doc
Ngày tải lên :
19/02/2014, 16:20
... underscoring the importance of
the interactions between the Tyr OH and the side
chains of Asn174 and Asp148 in TEV protease
(Fig. 4A). The importance of these interactions is fur-
ther supported by the ... effects
of the mutations. Hence, the results are consistent with
the predicted role for these residues (based on crystal
structure) in the interaction with substrate. The loss of
activity of the ... modeling, the Arg side chain of the substrate
may partially compensate for the loss of the Lys side
Table 1. Comparison of the specificity of TEV and TVMV proteases. The relative specificity constants...
- 10
- 523
- 0
Tài liệu Báo cáo "Effect of the preparation conditions on the properties of Fe-Pt nanoparticles produced by sonoelectrodeposition " pptx
Ngày tải lên :
13/02/2014, 03:20
... / VNU Journal of Science, Mathematics - Physics 25 (2009) 1-7
6
the magnetic squareness M
r
/M
s
reduced with J because of the presence of the Fe
3
Pt. Of cause, the kink
in the magnetic ... of the samples. J is the current density; t
on
is the deposition
time of a pulse; Fe, Pt are the atomic percent of Fe and Pt in the samples, respectively; H
c
is the coercivity; M
s
is
the ...
with diameter of 1.3 cm acted as both the cathode and ultrasound emitter (Sonics VCX 750). The
electroactive part of the sonoelectrode was the planar circular surface at the bottom of the Ti horn....
- 7
- 513
- 0
Tài liệu Báo cáo khoa học: Substrate specificity of the pseudouridine synthase RluD in Escherichia coli doc
Ngày tải lên :
18/02/2014, 16:20
... obtained with the transition at posi-
tions 1915 and 1917. These mutations had an effect on
the formation of pseudouridine exclusively at the
mutant position and did not affect either of the other
two ... by
Southern analysis. The rluD::cat strain had a full deletion
of both rluD and yfiH, together with their annotated pro-
moter sequences. The inserted cat cassette had a distance of
90 nt from the ... induces reorganization of the RNA [21]. An
ability of the RNA substrate to adopt the alternative
fold with a reverse Hoogsteen base pair is used by
RluA to recognize its substrate [21]. It is...
- 8
- 596
- 0
Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
Ngày tải lên :
18/02/2014, 16:20
... rigid
aromatic side chain at the P2 position of the substrate
in the case of papain [26,41].
Ervatamin-B shows Pro specificity at the P2 position
of the substrate. The S2 subsite of ervatamin-B is lined
with ... proton from the catalytic Cys pres-
ent at the N-terminus of the helix to the His of the
dyad [19,50,51]. The first stage of catalysis is mediated
by the highly active thiolate ion of the Cys. Biochemi-
cal ... erva-
tamin-A, the P3 moiety of both the molecules of the
asymmetric unit runs along the extended backbone of
residues 65–64 and is partly exposed to the solvent
(Fig. 2B). On the other hand, the P3...
- 14
- 634
- 0
Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
Ngày tải lên :
18/02/2014, 17:20
... with the substrate aglycone but do
not participate in the binding of the substrate glycone.
Therefore, an implication of these results is that the
interactions with aglycone do not affect the ... Thus, the effect
of the alteration of the glycone structure in the stabil-
ity of the ES
à
complex (DDG
à
) for the wild-type and
mutant Sfbgly was determined using the k
cat
⁄ K
m
for
the hydrolysis ... degree of polymerization equal to 1.
Linear free energy relationships
The effect of the alteration of the substrate structure on the
stability of the complex ES
à
was evaluated using the follow-
ing...
- 12
- 731
- 0
Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
Ngày tải lên :
19/02/2014, 02:20
... phosphorylation of the wild-type. Relative
phosphorylation expressed as a relation of the percentage of dThd
phosphorylation of the corresponding mutant is given in paren-
theses. The substrate concentration ... treatment of cancer and viral
diseases because they catalyse the addition of the first
phosphate group to the nucleoside analogue. This
primes the nucleoside for further phosphorylation to
the corresponding ... true, a profound
effect on the catalytic rate should be evident from
these mutations. Binding of the substrates to the
enzyme should not be altered to the same extent, as
reflected by the lower...
- 10
- 504
- 0
Tài liệu Báo cáo khoa học: Role of K22 and R120 in the covalent binding of the antibiotic fosfomycin and the substrate-induced conformational change in UDP-N-acetylglucosamine enol pyruvyl transferase docx
Ngày tải lên :
19/02/2014, 13:20
... wild-type enzyme, whereas the K22E mutant protein
failed to form the covalent adduct. On the other hand, the
K22V mutant protein requires the presence of UDPNAG
for the formation of the adduct indicating ... wall biosynthesis are
attractive targets for the development of new drugs [1]. The
biosynthesis of the cell wall component peptidoglycan (or
murein) commences with the transfer of the intact ... binding. The loop region of the upper domain
moves towards the active site of the enzyme, thus shielding
the substrates (and ligands) from bulk solvent. A detailed
thermodynamic study of this...
- 9
- 707
- 0
Tài liệu Báo cáo Y học: Importance of the amino-acid composition of the shutter region of plasminogen activator inhibitor-1 for its transitions to latent and substrate forms pdf
Ngày tải lên :
22/02/2014, 07:20
... 74% of the cases;
residue 186 an Asn in 87% of the cases; and residue 334 a
His in 80% of the cases [3]. In addition, residue 54 is a Pro in
89% of the cases. The importance of the identity of the
residues ... [4–6]. The subsequent RCL insertion into
b sheet A therefore results in an < 7-nm translocation of
the proteinase from the position of its initial encounter with
the RCL to the other pole of the ... we reach the
conclusion that the substitutions in the shutter region affect
the rate of latency transition by affecting the rate of passage
of the RCL through the gate region. Based on the amino-
acid...
- 10
- 431
- 0
Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
Ngày tải lên :
22/02/2014, 07:20
... different
flavonoids. The addition of a flavone (apigenin), of a
flavanonol (taxifolin) and of a flavan-3-ol (epicatechin)
(Fig. 8) did not alter the EPR spectra of the enzyme
indicating the absence of binding to the ... definition of some of the catalytic steps. Binding of
the various flavonols to the active site metal centre is found
not to require the presence of dioxygen and occurs without
formal reduction of the ... reaction
is rather unusual in that it involves the cleavage of two
carbon–carbon bonds and the concomitant production of
carbon monoxide. The stoichiometry of the process is such
that 2 mol of substrate...
- 9
- 517
- 0
Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
Ngày tải lên :
22/02/2014, 07:20
... For
comparison, the amount of GroEL was determined in the
same extracts using anti-GroEL Ig. It has been previously
shown that at the beginning of the stationary phase the rate
of synthesis of heat-shock ... respectively. In order to
estimate the ratio between the amounts of UP12 and the
total amount of protein in the extracts, a series of samples
containing determined amounts of the purified UP12 were
separated ... the deletion of the UP12 encoding gene increased
the sensitivity of the mutant to CCCP (Fig. 6), it did not have
any detectable effects on the sensitivity to mitomycin C,
unlike in the case of...
- 9
- 548
- 0
Báo cáo khoa học: Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per-Arnt-Sim domain kinase PASKIN ppt
Ngày tải lên :
06/03/2014, 00:21
... translocation of UDP-
glucose pyrophosphorylase 1 to the plasma membrane,
where it increases cell wall glucan synthesis at the
expense of glycogen storage. In the absence of PSKs,
glycogen rather than ... (data not shown).
On the basis of the known functions of the
PASKIN-related FixL oxygen sensor in bacteria and
the PASKIN orthologues in yeast, and considering the
lack of any obvious phenotype ... microarray of 1176 peptides in the presence of [c-
33
P]ATP. The magnified inset shows an example of the results obtained
after detection by phosphorimaging. (B) Peptide sequences of the 30 most...
- 12
- 353
- 0