pluripotent cell derived glial precursor cells for the delivery of therapeutic proteins to the central nervous system
Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders
... Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells share their ... source of cells and the potential to derive the cell type of interest together with the possibility to enrich for genetic modifications during the dividing stem cell state 1.1.1 Human pluripotent ... available, and most cells in the central nervous system are post-mitotic, which renders them unsuitable for genetic modifications However, the use of patient -derived stem cell populations offers an unlimited...
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Genetic engineering and surface modification of baculovirus derived vectors for improved gene delivery to the central nervous system
... vectors for gene delivery to the nervous system has been limited by their ability to transfer genes only to dividing cells, although they are well suited for on-site delivery to neural precursors for ... SURFACE MODIFICATION OF BACULOVIRUS DERIVED VECTORS FOR IMPROVED GENE DELIVERY TO THE CENTRAL NERVOUS SYSTEM YANG YI (B Eng.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY GRADUATE ... each of which has the potential to become protonated The branched form of PEI has yielded significantly greater success in terms of cell transfection, and is therefore the standard form of PEI for...
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Development of hybrid promoters and viral vectors for improved gene delivery to the central nervous system
... selection of promoters The gene delivery vector that carries and transfers the gene into the nuclei of target cell is another key Chapter Introduction issue of gene therapy The tropism of the vector ... nontarget cells Neurons are the major functional cells in the nervous system, carrying out the fundamental tasks of receiving, conducting and transmitting signals Therapeutic protection of these cells ... increase the difficulty of gene delivery In sight of these considerations, special attention has to be paid to the gene delivery system, especially to the safety issue which is related to the vector...
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Báo cáo khoa học: Planarian peptidylglycine-hydroxylating monooxygenase, a neuropeptide processing enzyme, colocalizes with cytochrome b561 along the central nervous system pptx
... Expression of PHM in the planarian eyes The planarian eye is composed of two types of cells, namely photoreceptor cells and pigmented cells [45] The pigment cells form an eye cup and the photorecepFEBS ... acquired the neuroendocrine vesicles equivalent to those seen in the present-day neurons of higher animals by the time of the emergence of the Plathelminthes in the evolutionary history The eye of the ... In the stalk region of the photoreceptor cells, neuropeptides may be synthesized and work to modulate the transmission of light stimulus To clarify the role of the amidated neuropeptides in the...
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báo cáo hóa học: " Temporal expression and cellular origin of CC chemokine receptors CCR1, CCR2 and CCR5 in the central nervous system: insight into mechanisms of MOG-induced EAE" pptx
... around inflammatory foci in the spinal cord of rats with EAE, thus identifying potential determinants for trafficking of these cells to the CNS These findings are discussed in relation to therapeutic ... form of the GSA/B4+ cells, indicating that these chemokine receptors are expressed by cells of the macrophage/microglia lineage, but not by T cells or astrocytes (Fig 4A, 4B, 4C) Distribution of ... phenotype of chemokine receptor mRNA expressing cells in MOG-EAE Cellular Cellular phenotype of chemokine receptor mRNA expressing cells in MOG-EAE High magnification bright-field photomicrographs of...
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Section III - Drugs Acting on the Central Nervous System docx
... in the Central Nervous System The capacity to integrate information from a variety of external and internal sources epitomizes the cardinal role of the CNS, namely to optimize the needs of the ... Nonneuronal cells include the macroglia, microglia, the cells of the vascular elements (including the intracerebral vasculature as well as the cerebrospinal fluid-forming cells of the choroid ... receptors (in the retina, inner ear, olfactory epithelium, tongue, or skin) transmit first to primary relay cells, then to secondary relay cells, and finally to the primary sensory fields of the cerebral...
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Báo cáo sinh học: "The long term effects of chemotherapy on the central nervous system" docx
... are planned that further reduce the dose of craniospinal radiation to 1,800 cGy, virtually no attention has been paid to the possible contribution of the chemotherapy to the cognitive decline ... were found to be toxic to neural progenitor cells even in low doses by Noble and colleagues [1] The greatest risk factor for radiation-induced cognitive decline is young age at the time of treatment ... instead For example, attempts to reduce the dose of radiation to the brain and spinal cord from 3,600 centiGray (cGy) to 2,400 cGy led to the development of a protocol in which reduced craniospinal...
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Báo cáo sinh học: "Systemic 5-fluorouracil treatment causes a syndrome of delayed myelin destruction in the central nervous system" doc
... TUNEL+ cells (which are apoptotic cells) were very rare, but such cells were frequently found in the SVZ, DG and CC of animals receiving chemotherapy In the SVZ and DG, the majority of TUNEL+ cells ... exposure to 5-FU In the CC, the reduction in apparent division of Olig2+ cells was proportionate to the overall reduction in all BrdU+ cells In the SVZ, there was an enhanced reduction of DCX+ cells ... contribute to the effects of chemotherapy on cognition The ability of irradiation to the CNS to suppress the generation of new neurons in the hippocampus has been suggested to be relevant to the understanding...
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Báo cáo sinh học: "How chemotherapy damages the central nervous system Christina A Meyers" ppsx
... hippocampus and the corpus callosum), primarily in progenitor cells and oligodendrocytes To determine whether these findings resulted in functional impairment, they investigated the auditory brainstem ... used chemotherapy agents (carmustine, cisplatin and cytosine arabinoside) were more toxic to CNS progenitor cells and nondividing oligodendrocytes than to many cancer cell lines in vitro They also ... injury due to cancer treatment The knowledge gained from this and other studies will guide the development of translational clinical research to protect the nervous system from injury, to better...
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Báo cáo khoa học: "Immunohistochemical study of constitutive neuronal and inducible nitric oxide synthase in the central nervous system of goat with natural listeriosis" potx
... of nNOS and iNOS in the nonneurological control brain Both nNOS and iNOS immunoreactivity was recognized in the brain of control goats No inflammatory cells were found in the brain sections of ... substituted (Fig 1, D) These findings suggest that neuronal cells including neurons, choroid plexus cells and some neuroglial cells may constitutively express both iNOS and nNOS in the non-neurological ... and autoimmune disease of the central nervous system Proc Natl Acad Sci U.S.A 1995, 92, 5312-5316 Johnson, G.C., Fales, W.H., Maddox, C.W and RamosVara, J.A Evaluation of laboratory test for confirming...
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báo cáo khoa học: "Isolated angiitis of the central nervous system with tumor-like lesion, mimicking brain malignant glioma: a case report and review of the literature" pdf
... presentation of primary angiitis of the central nervous system Ann Rheum Dis 2008, 67:1732-1735 doi:10.1186/1477-7819-9-97 Cite this article as: You et al.: Isolated angiitis of the central nervous system ... informed consent was obtained from the patient for publication of this Case report and any accompanying images A copy of the written consent is available for review by the Editor-in-Chief of ... (d) Page of non-tumoral texture was palpated by the surgeon, the lesion was subtotally resectted (Figure 2a) Intraoperative pathologic examination showed no tumor cells but inflammatory cells and...
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báo cáo khoa học: "In vivo observation of gold nanoparticles in the central nervous system of Blaberus discoidalis" potx
... dependence of gold nanoparticle uptake into mammalian cells Nano Letters 2006, 6:662-668 28 Begley DJ: Delivery of therapeutic agents to the central nervous system: The problems and the possibilities ... known that the nanoparticles will interact with the proteins present in the biological system, i.e the material surrounding the nanoparticles are proteins present in the nervous system of the cockroach ... surrounds the nanoparticles due to its negative surface charge In terms of defense mechanisms, when a foreign object enters the biological system, the response of the immune system is to block further...
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The role of hydrogen sulfide in the central nervous system implications in the treatment of alzheimers disease
... for the treatment of AD Majority of them are prescribed to alleviate neuropsychiatric symptoms without targeting underlying pathological mechanism Hence, a lot of efforts have been put into the ... positively linked to AC, stimulation of A2A receptors by HENECA resulted into the increase in intracellular cAMP levels Similar to the first part of the studies, NaHS inhibited the cAMP production ... 1.1 The trio of gasotransmitters In recent few decades, the scientific community has witnessed the rise of a whole new class of gaseous biological mediators in mammalian cells The size of this...
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Chemical drug assisted gene transfer a sensible approach to improve transgene expression in the central nervous system
... therapy: gene delivery systems (vectors), regulatory elements and therapeutic genes The vectors refer to the carriers of transgene into target cells, and they have been commonly divided into viral ... components to generate a safe and effective way for the delivery of genes into the patient and subsequent treatment of the disease by the transgene In the current stage of gene therapy, most efforts ... compared to other non-viral vectors Gene delivery using PEI involves condensation of DNA into compact particles, uptake into the cells, release from the endosomal compartment into the cytoplasm,...
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INVESTIGATION OF MEMBRANE PROPERTIES IN THE CENTRAL NERVOUS SYSTEM OF DROSOPHILA MELANOGASTER STUDIED BY FLUORESCENCE CORRELATION SPECTROSCOPY
... showing the arrangement of aCC/RP2 motor neurons within the central nervous system of stage-16 embryo FCS measurements were done on the upper and bottom membranes of aCC/RP2 motor neurons The bottom ... severe distortion to the autocorrelation curves [77] Strong deformation of the ACF curves at large delay time, τ, due to instability of the system such as movement, or slight displacement of the laser ... statistics, the variance is equal to the mean value Therefore, the standard deviation (σ) is equal to the square root of the mean value Although it is important to minimize the number of molecules...
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The study of the mediation of ohanin, a king cobra (ophiophagus hannah) toxin through the central nervous system
... mixtures of proteins and are stored in modified salivary glands at the back of the head These proteins can potentially be a mixture of neurotoxins (which attack the nervous system) , hemotoxins ... the brain, as this might be useful to the understanding of the control of locomotion and pain responses The identification of the receptor(s), and the identification of the specific regions of ... chemical information via their forked tongues, which pick up scent particles and transfer them to a special sensory receptor (Jacobson's Organ) located in the roof of its mouth When the scent of a...
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The central nervous system
... Brodmann performed an extensive study of the microscopic anatomy the cytoarchitecture of the cerebral cortex and divided the cortex into 52 separate regions on the basis of the histology of the cortex ... part of the auditory brain stem pathway Neurons of the inferior colliculus project to the thalamus, which then sends auditory information to the cerebrum for the conscious perception of sound The ... are the axons of these neurons The motor neuron that causes contraction of the big toe, for example, is located in the sacral spinal cord The axon that has to reach all the way to the belly of...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... of foot faults (SOFFF) in the forelimbs is defined as the number of faults of right and left forelimbs, whereas the differentiation of forelimb foot faults (DOFFF) is the difference between the ... coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant for four weeks, B: caspase III+ cells took the glial morphology ... related to modulation of the class I MHC, mediated by NSC -derived neurotrophins in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells...
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Use of IPs cell derived neural stem cells as a cellular vehicle for glioma and breast cancer therapy
... generation of large quantities of stem cells difficult One of the most-well studied is the adult hematopoietic stem cells, which give rise to all the blood cells Hematopoietic stem cells are in ... to cancer gene therapy 21 1.3.5 Current status of gene therapy for glioma and breast cancer 22 1.4 Stem cells 1.4.1 Adult stem cells 1.4.1.1 Neural stem cells 1.4.2 Induced pluripotent stem cells ... the inability of vectors carrying the suicide gene to reach invasive tumor cells distant from the tumor bulk as well as inefficient spread of the vectors within the tumor With the discovery of...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... cells [32] Moreover, heme induces the expression of the a-globin gene in K562 human erythroleukemia cells [33] and the number of hemoglobin-producing cells in YN-1 cells [27,34] Conversely, the ... are required to address the role of heme degradation in the population of hemoglobin-positive cells To the best of our knowledge, there has been no report on the promoter function of the human HO-2 ... It is therefore of significance to study the regulation of HO-2 expression in human cells under hypoxia In the present study, we have analyzed the effect of hypoxia on the expression levels of HO-1...
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