plant tissue culture sterilization methods
basic cell culture protocols methods in molecular biology - cheryl d. helgason, cindy l. miller
Ngày tải lên :
08/04/2014, 12:50
... immerse slides in
ethanol, and in the tissue culture hood, individually pull out and flame. Place
individually into each 24-well dish.
13. 24-Well tissue culture plates (Falcon, Becton Dickinson ... Culture Techniques 1
1
From:
Methods in Molecular Biology, vol. 290: Basic Cell Culture Protocols, Third Edition
Edited by: C. D. Helgason and C. L. Miller © Humana Press Inc., Totowa, NJ
1
Culture ... control DNA as internal standard (b). Cell cultures A,
C, and E are mycoplasma positive; cell culture B is mycoplasma negative. The analy-
sis of cell culture D is not evaluable because the internal...
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cell and tissue culture
Ngày tải lên :
10/04/2014, 11:12
... xvi
CELL
AND
TISSUE CULTURE
Angela Martin
National Cell and Tissue Culture Centre, Bioscience Ireland, School of
Biological Sciences, ... (1995)
The European Tissue Culture Society
(ETCS) initiative on quality control of cell
lines.
~xperi~~nts
in
Clinical Cuncer
~esearch:
4:
210-211.
CELL
AND
TISSUE CULTURE
v1
1.4
1.5 ... viability
of
cells in culture. In:
Jakoby
WB
&
Paston
IH
(eds)
Cell
Culture,
vol.
11,
pp.
141-151.
Academic
Press,
New
York.
CELL
AND
TISSUE CULTURE
X
5.3
5.4
5.5...
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introduction to cell and tissue culture
Ngày tải lên :
11/04/2014, 09:48
... 9
Chapter 2¡ª
Setting Up a Cell Culture Laboratory
Space Requirements
Ideally, the space allocated for the tissue culture laboratory should be one dedicated to tissue culture
functions exclusively, ... types of culture may stock a dozen or more different types of culture dishes
for specialized uses.
The type and configuration of culture vessel chosen will depend on the purpose of the culture. ... standard teaching laboratory for tissue culture using bench top
hoods and CO
2
cylinders. This setup requires a minimum of construction. A biosafety cabinet or tissue
culture hood, while useful,...
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tissue culture in the home kitchen
Ngày tải lên :
20/04/2014, 16:35
...
22101-0850 ISBN 0-947946-22-5 paperback
18. Methods in Plant Tissue Culture by Paul J. Bottino, 1981, 72 pages.
19. Experiments in Plant Tissue Culture by John H Dodds and Lorin W. Roberts, ... sow 3 or 4.
Transferring plantlets for further growth
Pinguicula heterophylla in culture
tranferring plantlets
plants in-vitro and also planted out
I have kept my cultures under coolish ... for home tissue culture, Part I, Brian Johnson, CPS Journal 14, 18-19.
2. Achieving the sterile state for home tissue culture, Part II, Brian Johnson, CPS Journal 16, 9-10.
3. Tissue culture...
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Plant cell culture
Ngày tải lên :
26/05/2014, 13:08
...
ISBN o-89603-549-2 (alk paper)
I Plant cell culture- Laboratory manuals 2 Plant tissue culture- Laboratory manuals
I Hall, Robert D (Robert David), l958- II Series Methods m molecular btology (Totowa, ...
high-frequency plant regeneration in rice tissue culture. 2 fur Pflanzenphysiol
113,315-323.
18. Raghava-Ram, N. V. and Nabors, M. W. (1985) Plant regeneration from tissue
cultures of Pokkali ... suspension cultures requires careful attention to the sub-
culture procedure. Regular subculture, usually at 7-d intervals and use of the
Introduction to Plant- Cell Culture
11
3.2. Sterilization...
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Báo cáo " Cell suspension culture Panax ginseng C. A. Meyer: Role of plant growth regulators and medium composition on biomass and ginsenoside production " docx
Ngày tải lên :
14/03/2014, 10:20
... Ushiyama. 1999. Commercial
production of ginseng by plant tissue culture
technology. In: T.J. Fun, G. Singh, W.R. Curtis
(Eds.), Plant Cell and Tissue Culture for the
Production of Food Ingredients, ... suspension
culture of ginseng cell and some attempts have
been made to increase biomass and ginsenoside
yield of ginseng cell culture.
2. Materials and methods
Stock cell culture and culture ... saponin in tissue culture
of ginseng (P. ginseng C. A. Meyer). Russ. J.
Plant Physiol., 40: 784-788.
[11] Yu K.W. 2000. Production of the useful
metabolites through bioreactor culture of...
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CULTURE OF CELLS FOR TISSUE ENGINEERING potx
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22/03/2014, 15:20
... 30
Culture of Cells for Tissue Engineering, edited by Gordana Vunjak-Novakovic and R. Ian Freshney
Copyright
2006 John Wiley & Sons, Inc.
23
CULTURE OF CELLS FOR
TISSUE ENGINEERING
Culture ... Cell Culture 4
2.1. Primary Explantation Versus Disaggregation 4
2.2. Proliferation Versus Differentiation 4
2.3. Organotypic Culture 7
2.4. Substrates and Matrices 9
3. Isolation of Cells for Culture ... Freshney
CULTURE OF CELLS FOR TISSUE ENGINEERING
Gordana Vunjak-Novakovic and R. Ian Freshney, Editors
CULTURE OF EPITHELIAL CELLS, SECOND EDITION
R. Ian Freshney and Mary G. Freshney, Editors
CULTURE...
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biopolymer methods in tissue engineering
Ngày tải lên :
10/04/2014, 11:11
... designed to improve transplantation of cultured autologous keratinocyte
Fig. 3. The HYAFF
®
11 membrane is optically transparent and allows microscopic
observation of cells cultured on it. The film ... 46, 273–282.
6. Pachence, J. M. and Kohn, J. (1997) Biodegradable polymers for tissue engineer-
ing, in Principles of Tissue Engineering (Lanza, R., Langer, R., Chick, W., eds.),
Academic Press, ... polymers for tissue engineering in orthopaedics. Materials Science
Forum 250, 115–228.
9. Thomson, R., Yaszemski, M., and Mikos, A. (1997) Polymer Scaffold process-
ing, in Principles of Tissue Engineering...
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cancer cell culture, methods and protocols
Ngày tải lên :
10/04/2014, 11:11
... centrifugation.
3.4. Culture and Maintenance
3.4.1. Culture Initiation
1. With minced tissue, place aliquots of the cell aggregates into multiple tissue culture flasks.
Add a maximum of 3–4 mL of tissue culture ... Lines 93
93
From:
Methods in Molecular Medicine, vol. 88: Cancer Cell Culture: Methods and Protocols
Edited by: S. P. Langdon © Humana Press Inc., Totowa, NJ
7
Isolation and Culture of Melanoma ... following features of key relevance for
successful culture: (1) nonenzymatic or minimal dissociation of tumor tissue, (2) seed-
ing of cultures as explant and at high cell densities, (3) removal of...
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plant functional genomics, methods and protocols
Ngày tải lên :
11/04/2014, 10:10
... Linux OS.
Methods in Molecular Biology
TM
Methods in Molecular Biology
TM
Edited by
Erich Grotewold
Plant Functional
Genomics
VOLUME 236
Edited by
Erich Grotewold
Plant Functional
Genomics
Plant ... Phytophthora-infected plant
tissue could be of either pathogen or host origin. Thus, the challenge is to dis-
tinguish between the plant and Phytophthora EST populations using sequence
analyses. In this case, plant ... tags or ESTs)
are ongoing for a wide variety of plants and plant- associated microbes. Simi-
larly, ESTs generated from mRNA isolated from plant tissue infected with
microbial pathogens have emerged...
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