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Báo cáo y học: " The Role of Catalase in Pulmonary Fibrosis" pptx

Ngày tải lên : 12/08/2014, 13:22

... and histopathological analyses of lung tissues obtained by video-assisted thoracoscopy-guided lung biopsy or surgical lobectomy as previously described [17] All control lung specimens were obtained ... sample Lung catalase and glutathione peroxidase activity Frozen lung tissues were homogenized in lysis buffer and used for assessment of the activities of calatase and glutathione peroxidase using ... significant at p < 0.05 (StatView J 5.0, SAS Institute Inc., Cary, NC, USA) Results Catalase is decreased in human pulmonary fibrosis We first assessed whether the catalase activity is altered in human...

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Báo cáo y học: "Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array" ppt

Ngày tải lên : 11/08/2014, 08:21

... charts RS peer-reviewed the data interpretations All authors read and approved the final manuscript Acknowledgements We thank James Mobley and Ted Baginski for performing the in vivo phase of this ... were determined using a BCA kit Lung tissue homogenates were diluted with 50% assay diluent (provided in the Mouse Cytokine 10-Plex kit) and 50% TPER reagent to a final protein concentration of ... antibody was added to the appropriate wells and incubated for hour After washing, 100 μl Streptavidinphycoerythrin was added to each well and incubated for 30 minutes After a final wash, the plate...

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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf

Ngày tải lên : 12/08/2014, 18:20

... compounds in lungs Since the pulmonary levels of individual compounds of cigarette smoke are unknown, comparisons between the in vivo and in vitro situation are difficult to make However, the CSC ... smokers with and without COPD Downstream signaling pathways that are activated via the EGFR and regulate cell survival and proliferation include phosphorylation of mitogen activated protein kinases ... GSH/thiol provided by NAC protects cells against oxidative stress The aim of the present study was to analyze the effect of cigarette smoke on cell proliferation and wound repair using an in vitro cell...

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Tài liệu Báo cáo khoa học: Caveolin-1 inﬂuences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

Ngày tải lên : 19/02/2014, 00:20

... involved in ion channel activity, and that receptors present in lipid rafts may regulate the activities of proteins included in signal transduction cascades [14] More evidence for the association ... P, Ory D, Mulligan R, Gage FH, Verma IM & Trono D (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector Science 272, 263–267 47 Lois C, Hong EJ, Pease ... immunoreactivity Decreased levels of P2X7 receptor expression were detected in Caveolin1-deﬁcient mice as compared to wild-type mice (Fig 6A) Wild-type mice exhibited P2X7 receptor immunoreactivity...

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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt

Ngày tải lên : 11/08/2014, 08:21

... on the impact of bacterial and viral infections in type-1 or type-2 biased pulmonary inflammations like COPD or asthma, respectively In summary, our results provide further insight in the role ... in the role of TLRs in the immune response of the lung epithelium to viral and bacterial infections and their contribution to virus and bacteria induced exacerbations of pulmonary diseases Methods ... (PGN), 10 µg/ml S cerevisiae zymosan, 200 ng/ml MALP-2 (macrophage activating lipopeptide-2), 200 ng/ml Pam3CSK4 (palmitoyl-3cysteine-serine-lysine-4) (all obtained from Invivogen, (San Diego,...

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Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro" potx

Ngày tải lên : 11/08/2014, 08:21

... serum will halt cell growth and division thus eliminating any inhibitory action of our treatments on cell growth However, this practice did not adversely affect cell viability as measured by the MTT ... and Behavioural Aspects Edited by: Wonnacott S, Russell MAH, Stolerman IP New York: Oxford University Press; 1990:112-157 Parone PA, James D, Martinou JC: Mitochondria: regulating the inevitable ... collection (ECACC) The cells were maintained in Nutrient mixture F12 Ham supplemented with 10% fetal bovine serum, mM L-glutamine (Life Technologies, Paisley, UK), 50 μg/ml penicillin (Life Technologies),...

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Báo cáo y học: "Modulation of epithelial sodium channel (ENaC) expression in mouse lung infected with Pseudomonas aeruginosa" pps

Ngày tải lên : 12/08/2014, 18:21

... explain high altitude lung edema (HALE) [30,31] Several lines of evidence suggest that upregulation or downregulation of ENaC activity in the lung could be associated with lung infection In type ... regulator (CFTR) in CF cells to downregulate ENaC activity [37,38] Pseudomonas aeruginosa is a bacterium occuring naturally in a wide range of environments such as in soil, fresh and seawater, plants ... bacterial infection in vivo can lead to modulation of ENaC mRNA expression Recently, αENaC mRNA expression was found to be downregulated in the mouse lung after and 14 days of adenoviral infection [51]...

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Báo cáo y học: "B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren''''s syndrome" ppt

Ngày tải lên : 09/08/2014, 07:20

... HM: Evidence for coxsackievirus infection in primary Sjogren's syndrome Arthritis Rheum 2004, 50:2897-2902 54 Mariette X, Gozlan J, Clerc D, Bisson M, Morinet F: Detection of Epstein-Barr virus ... (Courtaboeuf, France) cDNA synthesis involved the use of Enhanced Avian HS RT-PCR Kit from Sigma-Aldrich (Saint Quentin Fallavier, France) BAFF and β-actin cDNA levels were determined by use of ... minutes to inactivate endogenous peroxidase activity Then slides were incubated at room temperature for 30 minutes each, with the use of an EnVision Detection Kit peroxidase/diaminobenzidine,...

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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf

Ngày tải lên : 12/08/2014, 11:22

... from viruses of the Eurasian-avian-like swine virus lineage [5,6] Since these swine viruses have in turn originated via complex genetic reassortments between swine, avian and human influenza viruses, ... these two H1N1 viruses, in order to provide insights into virus pathogenesis, which may in turn be relevant to therapeutic strategies for the treatment of influenza Methods Viruses The viruses used ... ferret lung in vivo, the two viruses replicate comparably in human type I alveolar epithelial cells and in ex vivo lung cultures [3] Arguably, the ex vivo lung data showing comparable viral tropism...

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Báo cáo y học: "Phenotypic alterations in type II alveolar epithelial cells in cell mediated lung inflammation" pdf

Ngày tải lên : 12/08/2014, 15:20

... internal milieu and the external environment, and the respiratory epithelium is the initial point of contact for respiratory viruses, airborne allergens and environmental pollutants [1] The major ... sterile phosphate buffered saline via the pulmonary artery until visually free of blood ml dispase (BD Biosciences, Heidelberg, Germany) was instilled into lungs via the tracheal catheter followed ... isolated from SPC-HA mice not receiving CD4+ T cell transfer (fold change = 1) 1000 day Gelsolin Array Tubulin Array day Gelsolin Array Vimentin Array day Tubulin Array Vimentin Array Genes associated...

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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx

Ngày tải lên : 12/08/2014, 15:21

... bone marrow and cord blood cells Leuk Res 2006 Plett PA, Frankovitz SM, Orschell-Traycoff CM: In vivo trafficking, cell cycle activity, and engraftment potential of phenotypically defined primitive ... analyses and drafted the manuscript JH participated in interpretation and critical review of data as well as the revision of the manuscript for important intellectual content SvE conceived the study ... trace and quantify these progenitor cells in the lung QDs have previously been used to label live cells for long-term multicolor in vivo imaging [22] These nanocrystals are taken into cells by endocytotic...

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Báo cáo khoa học: " Salmonella Typhimurium invasion of HEp-2 epithelial cells in vitro is increased by Nacylhomoserine lactone quorum sensing signals" ppt

Ngày tải lên : 12/08/2014, 18:22

... under varying conditions Using a RIVETmethod (Recombination-based In Vivo Expression Technology) to record SdiA activity in vivo, Smith et al observed SdiA activation during the Conclusions The ... vitro Materials and methods Bacterial strains The bacteria used in this study were the wildtype S Typhimurium ATCC 14028 and its isogenic sdiA mutant (ΔSTM1950: Kan-PT7) which was kindly provided ... instructions cDNA was synthesized immediately after RNA isolation, using Invitrogen’s SuperScript® II Reverse Transcriptase (Invitrogen, Ltd, Paisley, UK) according to manufacturer’s instructions PCR...

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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot

Ngày tải lên : 19/06/2014, 08:20

... not for citation purposes) Virology Journal 2005, 2:56 http://www.virologyj.com/content/2/1/56 Table 1: Duration of survival of HSV-1 infected scid mice Treatmenta Expt Virus PBS rabbit IgG NK-depletedb ... (wild-type) mice uniformly survived KOS-GFP infection (Fig 7C) In contrast, 0% of rag2-/- mice survived and their duration of survival was 25 ± days p.i Thus, the duration of survival of KOS-GFP-infected ... "low" natural cytotoxicity in vitro, because there is no evidence that these mice lack NK cell function in vivo [21,51] T cells that become activated in response to viral infections express the...

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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot

Ngày tải lên : 20/06/2014, 04:20

... not for citation purposes) Virology Journal 2005, 2:56 http://www.virologyj.com/content/2/1/56 Table 1: Duration of survival of HSV-1 infected scid mice Treatmenta Expt Virus PBS rabbit IgG NK-depletedb ... (wild-type) mice uniformly survived KOS-GFP infection (Fig 7C) In contrast, 0% of rag2-/- mice survived and their duration of survival was 25 ± days p.i Thus, the duration of survival of KOS-GFP-infected ... "low" natural cytotoxicity in vitro, because there is no evidence that these mice lack NK cell function in vivo [21,51] T cells that become activated in response to viral infections express the...

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Báo cáo khoa học: "Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in Large Intestines of C57BL/6 Mice" potx

Ngày tải lên : 07/08/2014, 15:20

... 43:1031-1037, 1998 Sandstrom O, Mahdavi J, El-Salhy M Effect of ageing on colonic endocrine cell population in mouse Gerontology, 44:324-330, 1998 Sandstrom O, Mahdavi J, El-Salhy M Age-related changes ... gastrointestinal endocrine 15, 16 cells in mouse strains Gastrointestinal endocrine cells are generally divided into two types, the round to spherical shaped close-typed cells, which are located in the ... large intestinal tract of C57BL/6 mice These results corresponded 24, 25, 28 well with those of previous studies However, the single use of CGA as an endocrine cell marker is not recommended, as...

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Báo cáo khoa học: " Immunohistochemical Study of the Endocrine Cells in the Pancreas of the Carp, Cyprinus carpio (Cyprinidae)" docx

Ngày tải lên : 07/08/2014, 15:20

... further subdivided into three regions from centrally to marginally, central, mantle and peripheral regions according to types of cell composition The pancreatic duct regions were also subdivided into ... streptozotocin in the mouse Virchows Archiv A Pathol Anat Histopathol 1988, 412, 225-230 J oh n s on , D E., N oe , B D an d Ba u e r, G E Pancreatic polypeptide (PP)-like immunoreactivity in the pancreatic ... light microscopy Cla ss ifica tion of pa n cre a tic re g ion s The distribution of IR cells was divided into four regions, 1) the principal and 2) secondary islets regions, 3) the exocrine regions...

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Báo cáo khoa học: "Role of mucosal mast cells in visceral hypersensitivity in a rat model of irritable bowel syndrome" ppt

Ngày tải lên : 07/08/2014, 18:20

... has visceral hypersensitivity We examined whether the visceral hypersensitivity in the IBS rat is related to degranulation of MMC In normal rats, doxantrazole had no effect on either the visceral ... AWR behavior or the pseudo-affective tachycardiac responses to rectal distension (Fig 4) However, the same dose of mast cell stabilizer effectively alleviated the visceral hypersensitivity in ... important pathophysiological factor in visceral hypersensitivity associated with IBS In experimental animals, the role of MMC degranulation in visceral hypersensitivity has been demonstrated; doxantrazole...

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Báo cáo y học: " Immunohistochemical Characteristics of Bone Forming Cells in Pleomorphic Adenoma" ppt

Ngày tải lên : 08/08/2014, 16:23

... mainly by direct deposition of the osteoid tissue produced by the modified myoepithelial cells and via partial endochondral ossification mode However, Takeda and Yamamoto [8] discussed bone formation ... stromal fibrocytes and parenchymal epithelial cells, while no myxochondroid or chondral tissue was evident in the surrounding tissue They discussed how the histopathological features suggested the...

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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx

Ngày tải lên : 08/08/2014, 21:20

... IgE levels in atopic individuals Surprisingly, oral LCs constitutively expressed the high-affinity receptor for IgE (FceRI) even in nonatopic donors.33 As mentioned previously, the oral mucosa ... Neutralization of cytokine activity showed that T-cell suppression was induced by IL-10 and TGF-b during SIT and in normal immunity to the mucosal allergens A deviation toward a regulatory or ... induced by specific immunotherapy suppresses IgE and synthesizes IgG4 In vitro studies demonstrate nonanaphylactogenic activity by blocking of IgE binding IL-10 reduces release of proinflammatory...

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Báo cáo y học: "Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis" doc

Ngày tải lên : 09/08/2014, 01:23

... synovium cells than in PBMCs, probably because RA synovium produced more endogenous IL-18 and responsed more strongly to IL-18 Although an IL-18-neutralizing activity was found in RA synovial ... disease activity Next, we compared the response to TH1-inducing cytokines of blood versus synovium cells from RA patients Judging from the effect on IFN-γ production, total RA synovium cells showed ... Quentin Fallavier, France) Preparation of synovium and cell cultures R40 Peripheral blood samples were obtained from 14 patients (2 men and 12 women) with RA who fulfilled the 1987 revised criteria...

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