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Báo cáo y học: " The Role of Catalase in Pulmonary Fibrosis" pptx

Ngày tải lên : 12/08/2014, 13:22

... acatalasemic mice may not be due to excessive extracellular oxidative stress, although this could be related to an insensitive way of measuring the oxidative stress Our result that catalase is decreased ... and p = 0.003, respectively), suggesting accelerated fibrinogenesis in the acatalasemic mice (Figure 7B and 7C) To assess whether acatalasemic mice exhibit excessive oxidative stress in the lungs ... for catalase Fibroblastic foci were exclusively negative for catalase (Figure 2E) Contamination by red blood cells does not contribute to catalase activity of the lungs Because high catalase levels...

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Báo cáo y học: "Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array" ppt

Ngày tải lên : 11/08/2014, 08:21

... homogenates from CD-1 mice at 24-hours after i.p Representative qualitative levels of cytokine protein expressions in Representative qualitative levels of cytokine protein expressions in lung homogenates ... 24-hour LPS-treated mice using ELISA (Figure 4) Discussion CD-1 mice are relatively sensitive to endotoxin and the relative effects of endotoxin (specifically LPS) on lung inflammation in CD-1 mice ... (1000 μg/ml) LPS exposure causes activation of both T cells and macrophages, while low dose (100 μg/ml) treatment evokes activation of macrophages only [8] Perivascular edema and accumulation of...

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Báo cáo y học: " Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione" pdf

Ngày tải lên : 12/08/2014, 18:20

... pathways that are activated via the EGFR and regulate cell survival and proliferation include phosphorylation of mitogen activated protein kinases (MAPK) and Akt/PI-3 kinase pathways Activation of the ... included as a positive control for activation of p38 and JNK Whereas ERK1/2 activation was observed at all concentrations of CSC both in NCI-H292 (Figure 6A) and PBEC (Figure 6B), activation of p38 ... cells (PBEC) were derived from bronchial tissue that was obtained from resected lungs, derived from patients that underwent lung surgery for lung cancer at the Leiden University Medical Center...

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Tài liệu Báo cáo khoa học: Caveolin-1 inﬂuences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells docx

Ngày tải lên : 19/02/2014, 00:20

... P, Ory D, Mulligan R, Gage FH, Verma IM & Trono D (1996) In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector Science 272, 263–267 47 Lois C, Hong EJ, Pease ... lungs Wildtype: Arrow indicates a negative alveolar epithelial type II cell and a neighboring positive type I cell Note the additional strong immunoreactivity of endothelial cells in a larger ... immunoreactivity Decreased levels of P2X7 receptor expression were detected in Caveolin1-deﬁcient mice as compared to wild-type mice (Fig 6A) Wild-type mice exhibited P2X7 receptor immunoreactivity...

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Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt

Ngày tải lên : 11/08/2014, 08:21

... macrophage activating lipopetide-2 (MALP-2) and cell supernatants were analyzed using multiplex ELISAs Results are shown as fold changes relative to untreated controls The figure shows representative results ... or endogenous TLR ligands on Th1 or Th2 driven pulmonary inflammations like COPD or asthma, respectively List of abbreviations COPD: chronic obstructive pulmonary disease; dsRNA: double-stranded ... of TLR activation, SAEC were stimulated with µg/ml E coli 0111:B4 LPS (ultrapure), 10 µg/ml S aureus peptidoglycan (PGN), 10 µg/ml S cerevisiae zymosan, 200 ng/ml MALP-2 (macrophage activating...

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Báo cáo y học: "Early Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro" potx

Ngày tải lên : 11/08/2014, 08:21

... acrolein It is thought that aldehydes mediate their effects by increasing the intracellular oxidative activity of the cells [5] However, the nature and mechanism of tobacco smoke-induced cell damage ... staining and by TUNEL labelleing of fragmented DNA, both relatively late events In addition, cytochrome C release from mitochondria and activation of the proapoptotic factor Bax was measured, both ... hours The number of TUNEL positive cells was further increased after 48 hours incubation There was also a concentration-dependent increase in cells staining TUNEL positive after treatment with 100%...

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Báo cáo y học: "Modulation of epithelial sodium channel (ENaC) expression in mouse lung infected with Pseudomonas aeruginosa" pps

Ngày tải lên : 12/08/2014, 18:21

... channels are 20 times less effective in driving amiloridesensitive current than the native channels and show differences in their biophysical properties [57,58] Gene inactivation or over-expression ... expression Densitometric quantitative analyses of the Northern blots were performed for the four mouse strains The relative expression at each time point was determined relative to uninfected animals ... that upregulation or downregulation of ENaC activity in the lung could be associated with lung infection In type I pseudohypoaldosteronism, a recessive genetic disease leading to a non-functional...

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Báo cáo y học: "B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren''''s syndrome" ppt

Ngày tải lên : 09/08/2014, 07:20

... survival was demonstrated previously [44] Figure cells from minor salivary glands Immunocytochemical analysis of BAFF expression in salivary epithelilal cells from minor salivary glands Positive ... positive lip biopsy (Chisholm score of or 4) The only patient with a negative lip biopsy also had anti-SSA antibodies and fulfilled AECG criteria No patients had evidence of other connective tissue ... with K562 The unit number showing relative BAFF mRNA level in each sample was determined as a value of BAFF cell equivalence normalized with β-actin cell equivalence Melting-curve analysis was performed...

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Báo cáo y học: " Systems-level comparison of host responses induced by pandemic and seasonal influenza A H1N1 viruses in primary human type I-like alveolar epithelial cells in vitro" pdf

Ngày tải lên : 12/08/2014, 11:22

... are up-regulated in response to HIV infection and that they inhibit production of new virus through suppression of the HIV long terminal repeat (LTR) promoter activity [21] Further work demonstrated ... than pdmH1N1 in ferret lung in vivo, the two viruses replicate comparably in human type I alveolar epithelial cells and in ex vivo lung cultures [3] Arguably, the ex vivo lung data showing comparable ... Interactions and Pathways [http://www.innatedb.ca/] Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method Methods...

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Báo cáo y học: "Phenotypic alterations in type II alveolar epithelial cells in cell mediated lung inflammation" pdf

Ngày tải lên : 12/08/2014, 15:20

... proven to be more informative than whole tissues in dissecting the roles of individual cell types in disease development in general, and immune regulation in particular Comparative genetic fingerprinting ... obliterative bronchiolitis Am J Respir Crit Care Med 2002, 165:1439-1444 Buckley S, Bui KC, Hussain M, Warburton D: Dynamics of TGFbeta peptide activity during rat alveolar epithelial cell proliferative ... according to the manufacturer's protocol Adoptive transfer of HA-specific CD4+ T cells Naïve CD4+ T cells from the spleens of TCR-HA mice were isolated by negative selection by AutoMACS using the CD4+...

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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx

Ngày tải lên : 12/08/2014, 15:21

... quantitation of QD-positive cells, 100 randomly selected fields of lung parenchyma were photodocumented from each mouse using confocal microscopy with a 63× objective lens The Vv of QD-positive cells was ... trace diverse types of cells following cell proliferation and differentiation for up to a week without any effects on cell activation or cell function [22,23,31] We used whole BMCs and BMderived ... Plett PA, Frankovitz SM, Orschell-Traycoff CM: In vivo trafficking, cell cycle activity, and engraftment potential of phenotypically defined primitive hematopoietic cells after transplantation into...

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Báo cáo khoa học: " Salmonella Typhimurium invasion of HEp-2 epithelial cells in vitro is increased by Nacylhomoserine lactone quorum sensing signals" ppt

Ngày tải lên : 12/08/2014, 18:22

... under varying conditions Using a RIVETmethod (Recombination-based In Vivo Expression Technology) to record SdiA activity in vivo, Smith et al observed SdiA activation during the Conclusions The ... Page of transit of the S Typhimurum RIVET strain through turtles colonized by the AHL-producing species Aeromonas hydrophila [15] On the other hand, SdiA activation was not observed during the ... the gastrointestinal tract of a guinea pig, a rabbit, a cow, five mice, six pigs, or 12 chickens [15] Interestingly, SdiA was activated in mice that were infected with the AHL-producing pathogen...

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Báo cáo sinh học: " Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot

Ngày tải lên : 19/06/2014, 08:20

... (wild-type) mice uniformly survived KOS-GFP infection (Fig 7C) In contrast, 0% of rag2-/- mice survived and their duration of survival was 25 ± days p.i Thus, the duration of survival of KOS-GFP-infected ... respectively) In contrast, treatment with CyP significantly reduced the duration of survival of HSV-1-infected scid mice (Fig 6B; p < 0.001; 14 ± 0.2 days p.i.) The reduced duration of survival ... NK cell cytotoxic activity relative to BALB/c mice and BALB/c scid mice (Fig 2A) Following ocular inoculation with × 105 pfu/eye, HSV-1 strain KOS replicated to high and equivalent titers in BALB/c...

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báo cáo hóa học:" Re-evaluating the role of natural killer cells in innate resistance to herpes simplex virus type 1" pot

Ngày tải lên : 20/06/2014, 04:20

... (wild-type) mice uniformly survived KOS-GFP infection (Fig 7C) In contrast, 0% of rag2-/- mice survived and their duration of survival was 25 ± days p.i Thus, the duration of survival of KOS-GFP-infected ... respectively) In contrast, treatment with CyP significantly reduced the duration of survival of HSV-1-infected scid mice (Fig 6B; p < 0.001; 14 ± 0.2 days p.i.) The reduced duration of survival ... NK cell cytotoxic activity relative to BALB/c mice and BALB/c scid mice (Fig 2A) Following ocular inoculation with × 105 pfu/eye, HSV-1 strain KOS replicated to high and equivalent titers in BALB/c...

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Báo cáo khoa học: "Regional Distribution and Relative Frequency of Gastrointestinal Endocrine Cells in Large Intestines of C57BL/6 Mice" potx

Ngày tải lên : 07/08/2014, 15:20

... Pathol, 26:488-498, 1989 El-Salhy M, Winder E, Lundqvist M Comparative studies of serotonin-like immunoreactive cells in the digestive tract of vertebrates Biomedical Res, 6:371-375, 1985 Guyton ... cholecyctokinin (CCK)-8 Materials and Methods Five adult male and female C57BL/6 mice (6-wk old, 21-26 body weight upon receipt) were acquired from the Charles River Laboratories (Yokohama, Japan) after ... Representative sections of each tissue were stained with hematoxylin and eosin for an optical microscopic examination of the normal gastrointestinal architecture The each representative section...

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Báo cáo khoa học: " Immunohistochemical Study of the Endocrine Cells in the Pancreas of the Carp, Cyprinus carpio (Cyprinidae)" docx

Ngày tải lên : 07/08/2014, 15:20

... pancreatic polypeptide Ca te go ry o f re lativ e fre qu e n c y The relative frequency of occurrence of each type of IR cell was placed into one of five categories, not detected (—), rare (±), ... digestive tract of the dog The regional distribution and relative frequency of glucagon-IR cells in the teleostean pancreas have been reported in the flatfish [55], Barbus conchonius [45], five ... Paralichthys olivaceus Gen Comp Endocrinol 2000, 120, 816 Ku, S K., Lee, H S and Lee, J H Immunohistochemical of glucagon-immunoreactive cells in the developing pancreas of the Korean native goat (Capra...

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Báo cáo khoa học: "Role of mucosal mast cells in visceral hypersensitivity in a rat model of irritable bowel syndrome" ppt

Ngày tải lên : 07/08/2014, 18:20

... or the pseudo-affective tachycardiac responses to rectal distension (Fig 4) However, the same dose of mast cell stabilizer effectively alleviated the visceral hypersensitivity in IBS rats By ... hypersensitivity in IBS, it is worthy to mention that antagonists of H1, H2, and H3 histamine receptors did not modify but 5-HT1A receptor antagonist effectively reduced the mast cell activatorinduced ... it was also reported that experimental activation of proteinase-activated Role of mucosal mast cells in IBS rat receptor-2 (PAR-2), which could be activated endogenously by mast cell tryptase...

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Báo cáo y học: " Immunohistochemical Characteristics of Bone Forming Cells in Pleomorphic Adenoma" ppt

Ngày tải lên : 08/08/2014, 16:23

... tissue, reacted positively to S-100 protein [Fig (5)] The S-100 positive cells coarsely proliferated in most stromal tissues, close to the bone tissues Furthermore, the S-100 positive products were ... bone formation in pleomorphic adenoma of minor salivary gland origin J Nihon Univ Sch Dent 1996; 38:102-104 Yates PO, Paget GE A mixed tumor of salivary gland showing bone formation, with a histochemical ... Pleomorphic adenoma of the parotid gland with extensive bone formation Pathol Int 2001; 51: 883-886 Thackray AC, Lucas RB Tumors of the major salivary glands Washington DC: Armed Forces Institure...

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Báo cáo y học: "Nature of Regulatory T Cells in the Context of Allergic Disease." ppsx

Ngày tải lên : 08/08/2014, 21:20

... suppress IgE production and type I skin test reactivity in accordance with promoting IgG4 and IgA types of antibody production that can block the responsiveness of IgE for allergens For a safe and ... production Furthermore, adoptive transfer of pDCs before sensitization prevented disease in a mouse asthma model.32 It was explained that pDCs did not induce T-cell division but suppressed the ... was further increased and correlated with the serum IgE levels in atopic individuals Surprisingly, oral LCs constitutively expressed the high-affinity receptor for IgE (FceRI) even in nonatopic...

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Báo cáo y học: "Decreased response to IL-12 and IL-18 of peripheral blood cells in rheumatoid arthritis" doc

Ngày tải lên : 09/08/2014, 01:23

... production by 47% for activated H-PBMCs versus 25% for activated RA-PBMCs This difference indicated that RA-PBMCs had a defective response to IL-18 even when these cells were activated by PHA/PMA While ... IL-18 of total RA synovium cells was similar to that of activated H-PBMCs, suggesting that these cells had been activated and were more sensitive to TH1-inducing cytokines IL-18BP has been identified ... However, later studies showed that exogenous IL-18 in the absence of IL-12 failed to drive the differentiation of naive T cells to Th1 cells [16] and that IL-18 was a potent inducer of IFN-γ from established...

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