... Mm01178820_m1 for murine transforming growth factor-b (TGF-b), Mm00802331_m1 for murine collagen III, Mm00433659_m1 for CXCL1/KC (keratinocytederived chemokine), Mm 00434228_m1 for murine interleukin-1b ... epithelial cells (Figure 2A) as well as in type II epithelial cells and alveolar macrophages in control lungs This was in line with findings by Kaarteenaho-Wiik and Kinnula [12] In contrast, bronchiolar ... fibrosis is characterized by the accumulation of extracellular matrix proteins, such as collagen III A variety of pro-fibrotic molecules are believed to play roles in the regulation of the fibrogenic...
... biomedical researc h in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately...
... Nagai S, Miki H, Ito I, Izumi T: Cytotoxic effects of cigarette smoke extract on an alveolar type II cell-derived cell line Am J Physiol Lung Cell Mol Physiol 2001, 281(2):L509-516 Rahman I, Mulier...
... (TTCAAGAGA), the reverse complement of the sense strand, and six thymidines as an RNA polymerase III transcriptional stop signal [sense oligonucleotide format: 5¢-T-(GN19)-(TTCAAGAGA)(91NG)-TTTTTTC] ... Caveolin-1-knockout (- ⁄ -) lungs Wildtype: Arrow indicates a negative alveolar epithelial type II cell and a neighboring positive type I cell Note the additional strong immunoreactivity of endothelial ... epithelial cell lines with properties more similar to those of AT I cells (R3 ⁄ 1, L2, E10) or AT II cells (A549) using western blot analysis Initially, we were only able to detect P2X7 protein...
... III, RayBiotech, Norcross, GA) following the manufacturer's instructions A complete list of cytokine antibodies present on the array is available under http://www.raybi otech.com/map/human_III_map.pdf ... µg/ml epinephrine, 10 µg/ml transferrin, µg/ml insulin, 0.1 ng/ml retinoic acid (RA), 6.5 ng/ml triiodothyronine, 50 µg/ml Gentamicin/Amphotericin-B (GA-1000) and 50 µg/ml fatty acid-free bovine ... double-stranded RNA and activation of NF-kappaB by Tolllike receptor Nature 2001, 413:732-8 Doyle S, Vaidya S, O'Connell R, Dadgostar H, Dempsey P, Wu T, Rao G, Sun R, Haberland M, Modlin R, Cheng...
... Nagai S, Miki H, Ito I, Izumi T: Cytotoxic effects of cigarette smoke extract on an alveolar type II cell-derived cell line Am J Physiol Lung Cell Mol Physiol 2001, 281:L509-L516 Lannan S, Donaldson ... biomedical researc h in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately...
... molecular cloning Proc Natl Acad Sci USA 1994, 91:247-251 O'Brodovich H, Canessa C, Ueda J, RafII B, Rossier BC, Edelson J: Expression of the epithelial Na+ channel in the developing rat lung ... Mobasheri A, Gonzalez T, Canessa CM, Martin-Vasallo P: Sodium transport systems in human chondrocytes II Expression of ENaC, Na+/K+/2Cl- cotransporter and Na+/H+ exchangers in healthy and arthritic ... beta-adrenergic agonist terbutaline on Na+ channel and Na+-K+-ATPase expression in alveolar type II cells Am J Physiol 1998, 275:L414-L422 Dagenais A, Denis C, Vives MF, Girouard S, Masse C, Nguyen...
... (100 µg/ml) Human erythroleukemia K562 cells stably expressing BAFF were grown in RPMI medium Available online http://arthritis-research.com/content/8/2/R51 supplemented with 10% FCS, penicillin ... line A significant increase was observed after stimulation with IFN-γ and IFN-γ + TNF-α for 48 Available online http://arthritis-research.com/content/8/2/R51 atin 19 and cytokeratin 903 but not ... Error bars indicate SEM All samples were processed in duplicate BAFF, B cell-activating factor Available online http://arthritis-research.com/content/8/2/R51 The present results confirm the recent...
... we found comparable up-regulation of the type III IFNs, IL28A, IL28B and IL29 following seasonal H1N1 or pdmH1N1 infection Although type I and type III IFNs bind to distinct receptors, they elicit ... IFN-related antiviral responses Type III IFNs, were more prominently induced by both viruses when compared with type I IFNs This highlights the significance of type III IFN signalling in the pathogenesis ... Figure Validation of IFN gene expression by real-time PCR Expression of type I (IFNb) and type III (IL29) IFNs were assessed by real-time PCR in pdmH1N1-, seasonal H1N1- and mock infected cells...
... Alveolar type II epithelial cells (AEC II, granular pneumocyte, type II pneumocyte, giant corner cell) represent a highly specialized subpopulation of the respiratory epithelium AEC II consist of ... histocompatibility complex, class II, DR alpha major histocompatibility complex, class II, DQ beta major histocompatibility complex, class II, DQ alpha major histocompatibility complex, class II, DR beta major ... with AEC II per mouse; ± standard deviation) AEC II surface: SPC-HA vs SPC-HA Transfer: P < 0,001), SPC-HA vs SPC-HA/TCR-HA (P < 0,0001), SPC-HA transfer vs SPC-HA/TCR-HA (P < 0,0001) AEC II perimeter:...
... used models of toxic, traumatic or ischemic tissue injury, and showed engraftment of both type II [20,21] and type I [19] pneumocytes from BMderived cells demonstrating the ability of these cells ... approximately 14.6% of the initially infused Lin- PCs homed to lung tissues and were potentially available for lung tissue regeneration and repair These findings showed firstly that very few progenitor ... progenitor cells will increase the number of these cells that home to injured lung tissues and become available for tissue regeneration and repair Interestingly, in the present study, approximately 10–13%...
... but recently a computerized analysis suggested that SrgE may be a secreted substrate of a type III secretion system [13] It has earlier been suggested that the mammalian gastrointestinal tract ... prediction of secreted substrates and identification of a conserved putative secretion signal for type III secretion systems PLoS Pathog 2009, 5:e1000375 Erickson DL, Nsereko VL, Morgavi DP, Selinger ... instructions cDNA was synthesized immediately after RNA isolation, using Invitrogen’s SuperScript® II Reverse Transcriptase (Invitrogen, Ltd, Paisley, UK) according to manufacturer’s instructions...
... Natural Killer (NK) cell deficiency associated with an epitope-deficient Fc receptor type IIIA (CD16 -II) Clin Exp Immunol 1996, 103(3):408-413 York IA, Roop C, Andrews DW, Riddell SR, Graham ... HSV-1 spread has been observed in IFN-α/ βR-/- IFN-γR-/- double knockout mice [22,30] Thus, the available evidence suggests that IFN-α/β and IFN-γ are important activators of Stat 1-induced resistance ... biomedical researc h in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately...
... Natural Killer (NK) cell deficiency associated with an epitope-deficient Fc receptor type IIIA (CD16 -II) Clin Exp Immunol 1996, 103(3):408-413 York IA, Roop C, Andrews DW, Riddell SR, Graham ... HSV-1 spread has been observed in IFN-α/ βR-/- IFN-γR-/- double knockout mice [22,30] Thus, the available evidence suggests that IFN-α/β and IFN-γ are important activators of Stat 1-induced resistance ... biomedical researc h in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately...
... isolated oral LCs expressed significantly higher amounts of major histocompatibility classes I and II, as well as costimulatory molecules CD40, CD80/B7.1, and CD86/ B7.2 FceRI expression on oral ... cells is by blocking B7/CD28 Also, IL-10 inhibits DC maturation and leads to reduced MHC class II and costimulatory ligand expression Thus, IL-10 not only generates tolerance in T cells, it also...
... levels by ELISA IFN-γ levels were measured by quantitative sandwich ELISA, using a commercially available ELISA kit (DuoSet ELISA Development System human IFN-γ, R&D Systems) The detection limit ... induced by IL-12 (1 ng/ml) and IL-18 (5 ng/ml) was reduced by IL-18BP in a dose-dependent manner Available online http://arthritis-research.com/content/6/1/R39 Figure (a) (b) 10 IFN-γ (ng/ml) IFN-γ ... is known to produce significant amounts of IL-18 spontaneously [21] Addition of IL-18BP could Available online http://arthritis-research.com/content/6/1/R39 Table Figure IFN-γ production by PBMCs...