... obsessed about scoring in games, and inthe backseat of a car. At the point when boys and girls begin deciding the trajectories of their careers, girls start to lose interest in pursuits that ... Brizendine, And in loving memory of Louise Ann Brizendine T he F emale B rain machinery she needs to live outside her mother’s body. But the major-ity ofthe brain development that determines ... response. The girls will think that if they do it just right, they’ll get the reaction they expect. It’s the same kind of instinct that keeps a grown woman going after a narcissistic or other-wise...
... proteindomain (E3int) representing the interface domain (resi-dues 343–470) of B. stearothermophilus E3. The use of the engineered E3int domain (27 kDa as the dimer)was introduced because the high ... investigate the basis ofthe interaction of E2p with E3 inthe assem-bly ofthe pyruvate dehydrogenase multienzyme complex of Bacillus stearo-thermophilus. Thirteen ofthe 55 amino acids inthe PSBD ... determined here (seeabove) are probably due to the use of uniformly15N-labelled PSBD and the inclusion of additional resi-dues inthe construct at the N-terminus ofthe domain. In particular, the...
... Vietnamese. At the beginning of each interview, the students were explained clearly, explicitly and unambiguously about the nature of the study. During the interview, the researcher modified the questions ... ofthe teachers often used the same six steps in organizing group work as these might be the basic steps needed for it. Inthe interview, almost all ofthe teachers thought that providing the ... Additionally, the current body of research mainly focuses on the product of group work implementation. Therefore, the researcher wants to investigate the use of group work in teaching speaking of English...
... exceed the lesser of 10% ofthe value of the gift and $75, then the value ofthe advantage is considered to be zero. For example, if the price to attend the benefit dinner is $200 and the value of ... to plan.Dinner, gala or social - Coordinate an evening of dinner and dancing in support of HSFM. Benefit dinners are great for rewarding employees and for promoting their work inthe community. ... services received in exchange for a gift as the Advantage. Inthe above example the advantage is the value ofthe dinner, i.e. $40. In situations where the cost or portion ofthe cost is covered...
... oxygen of the main chain at Asp266 was bound to amino groups of main chain of Tyr269 and Ala270 by hydrogen bonds. The interactions should maintain the structure ofthe C-terminalregion. Inthe ... region on the opposite side of the N-terminal region on the molecular surface. Inthe wild-type enzyme, the side chain of Asp266 interacted with the amino group ofthe main chain at Glu268. The oxygen ... autolysis in the N-terminal region and/or the C-terminal region [12,13].We hypothesized that the divergence ofthe properties of ALP I from the alkaline subtilisins might depend on the structure of the...
... CaM-bindingdomains, and contains at least one conserved potentialCaM-binding motif [46]. Additionally, the binding of CaM at the C-terminus and the binding of NADPH at the extreme N-terminus ... including the dehydrogenase domain. Spe-cific binding ofthe FNR is mediated by a unique series of proline/serine-rich repeat motifs located in the C-terminus. For the integration into the Tic ... N-terminus, anchoring it inthe IEM, whereas the C-terminus ofthe protein projects into the stroma(Fig. 1) [34]. Two motifs can be located inthe C-termi-nal half ofthe stromal domain: (a) the...
... indicating that interaction with Smac is notrequired with respect to the role of HSP70 inthe inhi-bition ofthe release of Smac and apoptosis. The role ofthe ATP-binding domain of HSP70 in ... showingimmunoreactive bands ofthe expected sizes (Fig. 4B).Next, whether the protective potency of HSP70 wouldbe annulled by deletion ofthe ATP-binding domain or the peptide-binding domain ... transfectedC2C12 cells. Furthermore, there was no direct interac-tion between HSP70 and Smac proteins, and the ATP-binding domain of HSP70, rather than the pep-tide-binding domain, was essential for...
... with the biosyn-thetic pathway of neral in Car. lactis (Fig. 1). A previ-ous study has also shown that the oxidation of geraniol is one ofthe key steps inthe production of neral [12]. The ... that of other ADHs [22,30–32]. The highly conserved sequences of zinc-binding andNAD+-binding sites as compared to other ADHssuggest that Car. lactis GeDH belongs to the med-ium-chain dehydrogenase ... structural maintenance. The glycine-rich region(position 204–209, GLGGIG) is also conserved in Car. lactis GeDH (Fig. 5), and has been found in the NAD+-binding site in ADHs [34]. The absence of the probable...
... reducing the NADPH concentration by half, or increasing ittwofold, had no in uence on the yield of GDP-Fuc.Furthermore, reduced conversion ofthe intermediatewas observed inthe absence of dithiothreitol. ... Amsterdam, the Netherlands), which weregrown overnight in 10 mL Luria–Bertani medium containingkanamycin and chloramphenicol (also containing ampicillin in the case of double transformation). Inthe ... (alternatively spliced form of GMD-1), gmd-2 andger-1. The amounts of cDNA used inthe PCRs were normalized on the basis ofthe intensity of actin transcripts.GDP-fucose biosynthesis in invertebrates S....
... Yap1to the GSH1 promoter could be repressed by Cbf1, aDNA-binding protein that binds to elements in the vicinity of Yap1 binding site [52]. Alternatively, the increased reducing power of tsa1 ... that Yap1 isretained inthe nucleus inthe presence of H2O2andthereby it interacts with the target genes [11,46,47].This retention is dependent ofthe oxidation of Yap1cysteine residues by ... accumulation in the nucleus of cells shifted from glucose to glycerol,otherwise observed, inthe presence of glutathioneexternally added.Another striking point inthe adaptation of cellsto...
... result-ing in a chimeric protein designated gp130c.Inordertoinvestigate the role ofthe membrane-proximal domains of gp130 in receptor activation, the FNIII-domain deletionswere introduced into the ... domain inthe membrane-proximal part of gp130leads to a shift ofthe receptor areas involved in ligandbinding closer to the membrane, resulting inthe inability of the receptor chains to form ... membrane-proximaldomains of gp130 is limited to ensure the correct spacingbetween the CBM and the membrane, each ofthe domainswas replaced by the corresponding domain ofthe GCSFR. The replacement...
... taurine uptake in NIH3T3 mousefibroblasts is linear within the initial 20 min following the addition of 14C-labelled taurine. Using taurine uptakewithin the initial 20 min in NaCl medium containingextracellular ... prior to the initiation ofthein ux experiments, inorder to removeexcess unlabelled taurine, and t aurine uptake was f ollowed w ith t ime using [14C]taurine as outlined in Fig. 1. In o rder ... and the protein content. The taurine inux (nmolặgprotein)1ặmin)1) w as estimated as the slop e o f the cellulartaurine uptake plotted vs. time.Estimation ofthe taurine effluxTaurine efflux...
... located at the C-terminal end ofthe linker region between the LD and the PSBD, just before the beginning of helix 1 inthe PSBD [6].Preceding Met131 inthe linker sequence, there is a series of hydrophobic ... higher than that ofthe wild-type PSBD. In fact, the side-chains of lysine and arginine are significantlydifferent, both in length and inthe point (lysine) anddelocalized (arginine) positive charge. ... hydrogen bonds. These properties account for the favoured occurrence of arginine residues at binding inter-faces. Indeed, in a database analysis ofthe contributions of individual amino acid residues...
... genes to test the importance of the conserved Glu residues inthe EX7E motif of these proteinsby the stringent criterion of their ability to support cellgrowth. Our results indicate that ... importance in vivo of conserved glutamate residues in the EX7E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit ofthe first enzyme in glycosylphosphatidylinositol assemblyZlatka ... datashowing retention of function of various key mutants in vivorender a demonstration of misfolding, instability, or mislo-calization ofthe protein, or of impaired complex formationby the protein,...
... sequencessuggesting rapid spread of a single lineage [51]. Analysis of measles viruses circulating in Burkina Faso, before andafter a mass vaccination campaign, showed that the number of circulating lineages ... clusters, assisting classical epidemiological investiga-tions and the tracking of chains of transmission [7,64].Competing interests The author(s) declare that they have no competing inter-ests.Authors' ... theseisolates. Inthe era before the widespread use of measlesvaccine, genotypes A, C1, and D1 were detected. Geno-type A virus includes the prototype Edmonston strain, the progenitor for most of the...