... terms ofthe donor’s gift. The duty resting on trustees to honour the terms of their trust and ensure that the objects ofthe charity prevail has always been seen as the primary means whereby the ... presumption that public benefit provision is wholly the responsibility of government has long gone.Government Policy The steady contraction of State responsibility for provision or delivery of public ... such missionary bodies should have the advancement of religion as their primary purpose.95Leaders of Voluntary ActionBy the end ofthe 19th century charities were the primary providers of health,...
... image ofthe products of D-glucose oxidation on PS-1 catalyst On the other hand, the reaction on Pt/SBA-15 catalysts with pH control not only showed a high conversion, but also high D-gluconic ... 183-190 189- From the results above, we found that both HNO3 and Pt/SBA-15 present a good conversion in the oxidation reaction of glucose. The conversion ofthe reaction reached 87.67% when ... of plantinum nanoparticles in the selective oxidation of glucose. When the reaction was performed without pH control on PS-1 catalyst, the conversion decreased significantly. The pH at the...
... coincide with the region that binds HR1 upon 6HB formation. The strong affinity of RC-101 for HR2 prevents the inter-action of HR1 and HR2, formation ofthe 6HB, andsubsequent fusion ofthe host ... regions of gp41 [12]. During 100 days of serial passaging, HIV-1strain BaL developed three cationic mutations in the presence of sublethal concentrations of RC-101 [14]. Of the three mutations, ... thus the dependence of viral replication onthe presence of RC-101 is not surprising. The cationic mutations andloss of glycosylated residues suggest an attempt by the virus to repel the cationic...
... substitutions imposed on the backbone conformation of WT* shows that the pattern of P37P38P19W45Fig. 1. Ribbon structure modelled onthe X-ray structure of humanubiquitin [32]. The position and ... solution of native protein wasunfolded by a 1 : 10 dilution to yield final concentrations of GdmCl near or above the midpoint ofthe equilibriumunfolding transition (concentrations of GdmCl in the range ... that the reduction in stability of thesemutants is largely manifested in perturbations to the unfolding rates with the degree of compactness of the TSE (aD) and linearity ofthe chevron plots...
... study, the effect of NaCl concentration on the kinetic constants and thermodynamic stability of VsEndA and VcEndA was investigated. In addition, the effects of reciprocal mutations of two non-con-served ... boundary conditions,and the resulting grid was adopted as the boundary condi-tion for one further focused calculation. The protein mole-cules occupied 90% ofthe box in the final calculations. The molecular ... activity conditions of the native enzymes [11]. The denaturation peaks weresymmetrical, except for some exothermic distortion of the thermograms after the denaturation peak,especially in the VcEndA...
... iden-tified as the major and the minor forms ofthe holo-ACP structure, onthe basis of their percentage contri-butions (65% and 35%, respectively) to the overallstructure ofthe protein. The structures ... against the fraction number (AU, arbitrary unit). (d) The apparent molecular masses of apo-ACP and holo-ACP were estimated on the basis ofthe linear regression ofthe fraction number ofthe molecular ... elucidates the interactions respon-sible for the conformational stability of PfACP. The size exclusion chromatography profile of PfACPshowed that the apparent molecular mass of PfACPmonomer is...
... assayed by varying the concen-tration of Tm in the presence of constant concentration of F-actin and myosin S1. In this condition, Tm inh ibits the ATPase activity as its concentration increases [33]. ... stability ofthe protein as a whole, ratherthan onthe position ofthe mutation in the polypeptide chain,as demonstrated by the biophysical assays. Our studies h aveprovided additional contributions ... f the mutant Tms. The mutations d id not affect the structure ofthe proteinas there was no significant alteration in the f unction or in the amount of th e s econdary s tructure. However, the...
... semiconductor, electron transfer occursbetween the semiconductor and the adsorbates. If the electron affinity ofthe adsorbates is larger than the workfunction ofthe n-type semiconductor, the ... characteristics of WO film mostly through the chemi-3cal nature of GB rather than the modification of physicalproperties of polycrystals.5. Conclusion The origin of binder effects onthe sensing ... than the exponential one since the desorption effect adds onthe exponential temperature de-Ž.pendence given by Eq. 5 . But the decrease of filmresistance is faster than the exponential one only...
... lattice constant and volume of unit cell increase with Zn content in the samples due to larger ionic radius of Zn2+ ion (0.82 Å) substituted for Ni2+ ion (0.78 Å). The X-ray density, the real ... Exchange interactions between magnetic ions through oxygen ion are superexchange interaction with antiferromagnetic nature. These interactions depend on bond distance and bond length. Normally ... each other below the freezing temperature Tf indicating the onset of blocking of clusters. The sample settles into the frozen state below temperature Tf. This behavior is attributed to the...
... with the reduced DMA ofthe AMY1 Y380A ‘sugar tongs’mutant, these effects may stem from allosteric regula-tion. The mutational analysis ofthe ‘sugar tongs’ in barleyAMY1 explored the role of ... Thisis the first demonstration of a function for a C domainfrom the large GH clan-H. The work contributes to the unraveling ofthe molecular basis of multivalentenzyme–polysaccharide interactions. ... connects b5 and a5 ofthe catalytic (b ⁄ a)8-barrelat the end ofthe aglycon-binding area ofthe active sitecleft. Earlier, significant deviation was found in thisregion between the backbone...
... host response depending onthe genetic constitution of the plant and the pathogen. In an incompatible inter-action, the pathogen remains localized at the primaryinfection sites that often are ... zone 1 (lanes 1,3,5,7) included necrotictissue from the lesions, whereas leaf material of zone 2 was collected ina region 7–14 mm away from the center ofthe lesions. Due to the expansion of ... collected from a narrowzone including the lesion and from a more distant zonearound single lesions of each plant. Whereas samples from the distant zone of control plants never contained necroticleaf...
... supportive of different contentions about harm, depending on the emphasis one chooses to give them. On one hand, it is clearfrom their reactions that the majority of youth regard their personalunwanted ... toconfirm a causal relationship.) On the other hand, one ofthe cautionary findings is that despite itsability to decrease exposure, the software seems far from foolproof.At least 18% ofthe ... av-erage of 24% ofthe health information and 91% ofthe pornography.Although such studies show possible weaknesses in the software ap-proach, they do not address the question of how the programs...
... obtained for the bilayerin the presence of 1 mM Zn ions, as a function of time, after the addition of A b (5 lM bulk concentration in the sample cell). Initial bindingoccurred mainly in the SM-rich ... facilitate peptide penetration of the hydrocarbon core ofthe membrane and subse-quent aggregation [22,37]. In order to test the effect of zinc addition in the present experiments on peptidebinding ... without loss of bilayer integrity. A structural interpretation of peptide interaction with the bilayer is presented based onthe results of simulation analysis ofthe PWR spectra.AbbreviationsAb,...
... ofthe peptide seemsto contribute to the effective anchoring ofthe tryptophanresidue.Concluding remarks The C-terminal 14 residue truncation of GGN4 abolished the biological activity ofthe ... tryptophanyl substitution at position 16 on the biological activity was confirmed for the native GGN4.Consistent with the results ofthe C-terminally truncatedGGN4, the D16W substitution in the native GGN4 ... amphiphilic in nature. The tryptophan side chain conformation was more clearlydefined in both ofthe environments than those of the other residues (Fig. 2D). However, the orientation of the tryptophan...