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pcr pcr amplification and manual sequencing

Báo cáo y học:

Báo cáo y học: "Establishing a gold standard for manual cough counting: video versus digital audio recording" ppsx

Báo cáo khoa học

... performed the study and the manual counting of the video and digital sound recordings and wrote the manuscript AW and JEE reviewed the final manuscript Acknowledgements Dr Yen Ha Yiew and Dr Barry Cheetham ... necessary to accurately manually count coughs and hence provide a gold standard for validation of novel cough monitoring systems We performed simultaneous overnight video and digital audio recordings, ... Figure cough counts plot of difference between Bland Altman versus mean cough count video and digital Bland Altman plot of difference between video and digital cough counts versus mean cough count...
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Báo cáo y học:

Báo cáo y học: "Common errors and clinical guidelines for manual muscle testing: "the arm test" and other inaccurate procedures" pdf

Báo cáo khoa học

... original work of Kendall and Kendall, Muscles: Testing and Function [12], and the modifications suggested by Goodheart in his Applied Kinesiology Research Manuals [1] Goodheart's and the I.C.A.K.'s ... and WHS both employ MMT and AK methods in their evaluation and treatment of patients Authors' contributions WHS and SCC conceived the research idea SCC constructed the literature review SCC and ... significant interand intra-examiner reliability as well as significant construct, content, concurrent and predictive validity [93] http://www.chiroandosteo.com/content/16/1/16 one test and to unknowingly...
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A parent mass filter algorithm for peptide sequencing from tandem mass spectra

A parent mass filter algorithm for peptide sequencing from tandem mass spectra

Tổng hợp

... approach and good speed and accuracy Peaks [20] was developed by Bin Ma et al, and is composed of mainly steps: (1) Preprocessing, (2) Candidate scoring (3) Refined scoring (4) Global and positional ... database, or by de novo sequencing of the peptide De Novo sequencing is sequencing of the peptide without prior knowledge about its sequence The difference is artificial, as de novo sequencing can be ... imperfect, sequencing will continue to be plagued with noisy or missing peaks Addition difficulties in sequencing arises from the facts that simple de novo sequencing is not mutation tolerant and are...
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Manual for installing NS2.29 under window XP

Manual for installing NS2.29 under window XP

Công nghệ thông tin

... the white area and then click on the OK button Click on the plus sign [+] next to HKEY_LOCAL_MACHINE to expand that section Then click on the plus sign [+] next to “Software” to expand that section ... with celeron 2.66 CPU and 512 M Memory, this step took around 75 minutes.) 10 22) Installing 11 12 Configure system variables and library paths 23) Installing is finished and the following window ... move over to the right side of the screen and scroll down until you see “Registered Owner” 10 Double click directly onto the words Registered Owner and input your new name, make sure no space...
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design manual for machine lubrication

design manual for machine lubrication

... 23 Design Manual for Machine Lubrication Initial Adjustment and Start-Up The system MUST be designed and installed per the Norgren “Micro-Fog Machine Lubrication Application Design Manual NT-1 ... use into the surrounding atmosphere Users are referred to OSHA Safety and Health Standards for limiting oil mist contamination and utilization of protecting equipment Centralized Lubrication Micro-Fog ... Figure 25 Littleton, CO USA Phone 303-794-2611 www.norgren.com 11 Design Manual for Machine Lubrication Rack and Pinion In a rack and pinion the bearing-inch total is 1/2 the projected area of the...
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Manual of diagnostic tests for aquatic animals 2010

Manual of diagnostic tests for aquatic animals 2010

Ngư nghiệp

... methods, and most of these laboratories have test programmes that use non-standard methods, or a combination of standard and non-standard methods In veterinary laboratories, even with the use of standard ... choice (both chemical and biological) and calibration, accuracy and acceptance limits of assay controls, reaction vessels and platforms, water quality, pH and ionicity of buffers and diluents, incubation ... larger aquatic animals and debris, an intermediate section that contains a finer matrix of sand and gravel, and the end section that contains fine sand c) For chlorination and de-chlorination,...
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Pasolink V4 Training Manual For Vietnam CT-IN NEC International Training, Ltd. Japan NECITR

Pasolink V4 Training Manual For Vietnam CT-IN NEC International Training, Ltd. Japan NECITR

Hóa học - Dầu khí

... error RX OPR LED RX side of IDU1 and ODU is online RX OPR LED RX side of IDU2 and ODU is online •When the IF cable between the IDU and ODU is disconnected IDU and ODU ALM LEDs start flashing Pasolink ... the DPU CH 1/2, CPU communication between ODU and IDU is lost OPR SEL NO1. Manually selected CH Mo.1 Automatic redundancy switch control NO2. Manually selected CH Mo.1 IDU LED Input data stream ... priorities Priority 1: Manual switching Control Manual switching is carried out by selecting from the front panel OPR SEL SW [No.1 –AUTO—No.2] When switched from here both TX and RX sides switches...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 1 pptx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 1 pptx

Ngư nghiệp

... Moina spp., daphnids, and decapsulated brine shrimp cysts for freshwater fish and prawn larvae, and Artemia biomass for lobster larvae, shrimp postlarvae and broodstock, and marine fish juveniles ... interest CLADOCERANS, NEMATODES AND TROCHOPHORA LARVAE 6.1 Daphnia and Moina 6.1.1 Biology and life cycle of Daphnia 6.1.2 Nutritional value of Daphnia 6.1.3 Feeding and nutrition of Daphnia 6.1.4 ... Rudi Bijnens, Magda Vanhooren and March Verschraeghen for their assistance with the layout of the manual Lavens, P; Sorgeloos, P (eds.) Manual on the production and use of live food for aquaculture...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 2 doc

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 2 doc

Ngư nghiệp

... with Ct and C0 being the cell concentrations at time t and 0, respectively, and m = specific growth rate The specific growth rate is mainly dependent on algal species, light intensity and temperature ... (upper diagram) and for Bürker (down left corner) For both, count the cells in the square and those which touch the top and left border (·) Do not count the ones touching the right and lower border ... consist of various trace metals and the vitamins thiamin (B1), cyanocobalamin (B12) and sometimes biotin Two enrichment media that have been used extensively and are suitable for the growth of...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 3 ppt

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 3 ppt

Ngư nghiệp

... increase and not by cell division The epidermis contains a densely packed layer of keratin-like proteins and is called the lorica The shape of the lorica and the profile of the spines and ornaments ... Selcođ and DHA-Super Selcođ) and two self home-made emulsified enrichment diets are given in Fig 3.13 and 3.14 ã Commercial emulsions Several emulsified diets are commercially available and based ... rinsed and concentrated before being fed to the predators Figure 3.13 EPA, DHA and total fatty acid content in two commercial emulsions (DHA Super Selcođ, DHA-SS and Super Selcođ, SS) and in...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 4 pptx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 4 pptx

Ngư nghiệp

... Wanigasekera, G., Simonek, S., Brownlee, D., Eiband, G and Cowan, J 1993 Ecological, physiological and genetic divergence of sexual and asexual (diploid and polyploid) brine shrimp (Artemia) Trends ... sinica Yaneng 1989: Central and Eastern Asia; · A persimilis Piccinelli and Prosdocimi 1968: Argentina; · A franciscana superspecies: Americas, Carribean and Pacific islands, including populations ... TEMPERATURE AND SALINITY TOLERANCE Both temperature and salinity significantly affect survival and growth, the effect of temperature being more pronounced A broad range of temperatures and salinities...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 5 docx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 5 docx

Ngư nghiệp

... below -18°C and above +40°C; a reversible interruption of the metabolism (= viability not affected) occurring between -18°C and +4°C and between ± 33°C and ± 40°C, with the upper and lower temperature ... value per cone and calculate mean value and standard deviation of cones = final value · Eventually leave hatching tubes for another 24 h, take subsamples again and calculate H% and HE for 48 h ... culturing and use in aquaculture Persoone, G., P Sorgeloos, O Roels and E Jaspers (Eds), Universa Press, Wetteren, Belgium, pp 261-269 Clegg, J.S and Conte, F.P 1980 A review of the cellular and developmental...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 6 docx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 6 docx

Ngư nghiệp

... 1970s and early 1980s, when many fish and shrimp hatcheries scaled up their production and reported unexpected problems when switching from one source of Artemia to another Japanese, American and ... and total (n-3) HUFA levels in enriched Artemia nauplii sampled in the laboratory (A) using a standard procedure and in three sea bream hatcheries (B) according to the in-house method (mean and ... 22:6n-3; pale bars) and a group receiving 12 h Selco®-enriched Artemia (6.4% 20:5n-3 and 3.3% 22:6n-3) in M and PL stage, followed by 24 h Selco®-enriched Artemia (21.3% 20:5n-3 and 12.7% 22:6n-3)...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 7 pptx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 7 pptx

Ngư nghiệp

... Artemia juveniles and adults Aquacultural Engineering 11: 107-119 Dhont J., Lavens, P and Sorgeloos, P 1993 Preparation and use of Artemia as food for shrimp and prawn larvae In: CRC Handbook of Mariculture ... of Artemia (ing.l-1) For the Dietrich and Kalle formulation, solutions A and B are prepared separately, then mixed and strongly aerated for 24h Dietrich and Kalle Instant Ocean ARC Solution A ... feeding and/ or increase water renewal; · 15 cm < T < 20 cm; maintain actual feeding ratio; · T > 20 cm; increase feeding ratio and/ or add food manually During next week: · T < 20 cm: stop feeding and/ or...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 8 pptx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 8 pptx

Ngư nghiệp

... pp Kungvangkij, P and Chua, T.E 1986 Shrimp culture: pond design, operation and management NACA training manual series No S Sirikarnpimp, Bangkok, Thailand 68 pp Lavens, P and Sorgeloos, P 1987 ... less than 7.5 and stimulating an algae bloom is difficult Using CaO and Ca(OH)2 will result in a quick pH rise to about 10 This way possible pathogens and predators will be killed CaO and Ca(OH)2 ... acid bottoms and when the pond water has a low concentration of Ca2+ and Mg2+ (note that in high saline waters Ca2+ and Mg2+ concentrations are usually high) If liming is the standard, exact...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 9 pptx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 9 pptx

Ngư nghiệp

... ng, and 63 ng respectively, and corresponds to 0.8% and 1.3% of the dry weight Within nauplii, the levels are relatively higher; (i.e around 3.9% and 3.4%, respectively) Specific levels of EPA and ... are respectively 6% and 17% in adults fed Dunaliella, and 18% and 32% in adults fed Rhodomonas In nauplii the levels of EPA, DHA and (n-3)HUFA are high, (i.e around 3.5%, 9.0% and 15%, respectively) ... to ensure adequate filtration and prevent losses due to the narrow and strong back current The longer the distance between the propeller and the net, the wider and shorter the net can be The...
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Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 10 pptx

Tài liệu Manual on the Production and Use of Live Food for Aquaculture - Phần 10 pptx

Ngư nghiệp

... Fisheries and Marine Science, Universiti Pertanian Malaysia, 214 pp Chao, N.-H., Lin, T.T., Chen, Y.-J and Hsu, H.-W 1995 Cryopreservation of late embryos and early larvae of oyster and hard clam ... also the production and use of live food organisms as feed for the developing larvae The present manual reviews and summarizes the latest developments concerning the production and use of the major ... harmful, (i.e B rubens lives on daphnids and hinders swimming and food collection activities) Brachionus is simply removed from the culture by flushing the water and using a sieve of appropriate mesh...
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Tài liệu Soft Computing for Optimal Planning and Sequencing of Parallel Machining Operations docx

Tài liệu Soft Computing for Optimal Planning and Sequencing of Parallel Machining Operations docx

Cơ khí - Chế tạo máy

... study of the optimal planning and sequencing for parallel machining operations The combinatorial nature of sequencing and the complication of having precedence and mode constraints make the problem ... Nee and A.N Poo, Integrated application of expert systems and neural networks for machining operation sequencing, in Neural Networks in Manufacturing and Robotics, Y.C Shin, A.H Abodelmonem and ... industrial manufacturing, only a handful of papers are found on sequencing machining operations for parallel machines [3, 22] The combinatorial nature of sequencing and the complication of having...
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Tài liệu Cyber Forensics—A Field Manual for Collecting, Examining, and Preserving Evidence of Computer Crimes ppt

Tài liệu Cyber Forensics—A Field Manual for Collecting, Examining, and Preserving Evidence of Computer Crimes ppt

Cao đẳng - Đại học

... include working with GTE, Perot Systems, and Arthur Andersen as a programmer, system analyst, project manager, and auditor Dedication Erienne, Kristina, and Andy Michael Jordan said it best, thus, ... would be needed and required by all potential readers and users in a single text Thus, this field manual presents specific and selected topics in the discipline of cyber forensics, and addresses ... planning, site and systems security, IT, financial and operational audits for an international clientele He has contributed numerous articles to audit−related publications and has authored and co−authored...
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