... 1–10 mM glutamine and 30 lg of the complex at 30 °C for 10 The sample was then boiled forand kept frozen at )80 °C for subsequent analysis To quantitate the amount of glutamate formed, 0.3 mL of ... Table Over-expression plasmids for Sno1p and Snz1p prepared in this study Symbols O, Z and H stand for Sno1p, Snz1p and His-tag, respectively Primer Name of plasmid Forward Backward Vector pSNO1 ... on 0.48 lmolÆmin)1Æmg)1 for Vmax and 3.4 mM for Km for glutamine depends on whether a proper synthetase partner and cosubstrate are present or not For example, the Vmax and Km values of imidazole...
... systematic problem formulation, as well as critical thinking and analysis, gave positive impact to my research Their demand for high standard did open up my eyes to a new horizon, and effected on ... identification approaches, and to design, evaluate and compare source identification schemes Our traffic analysisfor identifying traffic sources is closely related to web surfing The traffic sources we are identifying ... background on VPN and Tor traffic characteristics, and we review the current development of traffic source identification techniques, for further comparative analysis 2.1 Overview A traffic source is identified...
... (N/L) and 11 layers (L) {i.e 110 turns (N)} The total length of the conductor used for the side solenoid coils and the central solenoid coil was 95 meters and 80 meters respectively The coils ... the size of the solenoid coils, thickness of iron jacket, and amp-turns (NI) The optimum values of NI for the side and middle solenoid coils were obtained to be 12950 and 8250 respectively Based ... Design, fabrication, and characterization of a solenoid system 463 Table The optimized design parameters for solenoid coil with iron jacket Description Solenoid coil Coil type Coil size φ300 mm ×...
... could bring to information retrieval, information extraction, digital libraries and so forth, automatic identification of text types and genres could be particularly useful for problems that ... inferential model and reports evaluation; finally in Section we draw some conclusions and outline points for future work Background Although both Crowston and Williams (2000) and Shepherd and Watters ... Shepherd and Watters (1998) can be seen in terms of HTML tags (e.g tags for links and scripts) While content words or terms show some drawbacks for automatic genre identification (cf Boese and Howe,...
... by BLAST analysis [14] and multiple alignment using Clustal W [12] Phylogenetic analysis of the multiply aligned sequences was performed using protein distance and neighbor-joining analysis implemented ... implemented in the Phylip analysis package [15] Bootstrap analysis was also performed with 100 replicates and a consensus phylogenetic tree was determined For the phylogenetic analysis, positions in ... presented (in bold and boxed) and the other amino acids found at each position are aligned vertically above the consensus amino acid The coding strand sequence and the complementary strand corresponding...
... grateful to you for believing in me and letting me pursue my dreams The person I am today is because of you To my dear uncle and my aunt Khem Kandel and Laxmi Kandel: your support and friendship ... chemotherapeutic agent and in the mobilization and conditioning regimens for blood and marrow transplantation Reviewing the chemistry and pharmacology of cyclophosphamide is crucial for understanding its ... love and support provided me with the strength to persevere through the tough times and the long distances Thanks for being there for me v Abstract Bibek Parajuli IDENTIFICATION, KINETIC AND STRUCTURAL...
... not been possible: First and foremost my supervisor and mentor Dr Thomas Lufkin for being truly a teacher and a guide For showing me the ropes of doing science properly and teaching me its fundamental ... the correct question before looking for answers For being extremely patient and believing in me I truly believe he has made me a better scientist and a better human being and to paraphrase Newton- ... labeled RNA probes was performed following [99] and reported below DAY 1- Embryos were dechorionated and fixed in 4% Paraformaldehyde (PFA) for two hours at room temperature and dehydrated through...
... incidence was found in Iceland and Japan and highest in USA and France The overall prevalence was the lowest in Northern Ireland, UK and Finland, and the highest in Italy, Spain and Martinique The burden ... Thanks to Dr Sivalingam SP for his humor, kindness, and help Thanks to all staff of Deparment of Rheumatology and Immunology, Singapore General Hospital for their help and friendship ii Thanks ... Aw, Ms Cindy Goh, and the other staff Thanks for their kind help and encouragement I greatly acknowledge National University of Singapore for offering me the Research Scholarship and Singapore General...
... co-supervisor Dr Jerome Boulaire for his support and encouragement I would also like to thank Dr Poonam Balani for being a constant source of motivation I would like to thank Farah for help with the initial ... IFI30 promoter for transcriptional targeting in glioma and paves way for further research in the use of this promoter for in-vivo therapy VI List of Tables Table 2.1- PCR conditions for cloning ... chemotherapy is to control tumour growth and maintain satisfactory performance of patients for as long as possible The drugs procarbazine, lomustine, and vincristine and temozolomide have demonstrated...
... chair for Pharmaceutical industries for the assistance in completion of this work, Also, Dr Magdy Abdelhamid for his kind helping and efforts in drug analysis This Work was funded by Deanship for ... methanol, mixed well and vortexed for 15 minute and then centrifuged at 13000 rpm for 15 minutes The supernatant is taken and filtered using 0.22 Millipore disposable filters and then http://www.medsci.org ... from acute and chronic diseases Informed consent was obtained from each of the donors Blood samples were collected in heparinized vacutainers and centrifuged for at 5000 rpm The plasma and the buffy...
... K for h When the solution was cooled to the room temperature, the solution was immersed in deionized water for 48 h, and then the sulfonated copolymer washed with deionized water and dried for ... (DMAc) firstly, and then styrene, divinylbenzene (DVB) and benzoyl peroxide (BPO) were added to the solution and stirred with water bath at 343 K The dosage of PVDF, styrene, DVB and BPO was that ... and phase inversion, and all of the other reagents were analytical grade 2.2 Preparation of the PVDF-based composite membrane The PVDF powders were basified firstly, and the double bond was formed...
... (filer paper), BWX and PWSD The enzyme kinetic studies for cellulase and xylanase (Km and Vmax) were performed with 1-10 mg/ml of CMC and BWX, respectively [35] The crude cellulase and xylanase were ... literature for individual microorganisms: 27 kDa for a Thermotoga maritima cellulase [58]; 45 kDa for a B licheniformis xylanase [59]; 60 kDa for a Bacillus sp cellulase [60]; and 75 kDa for a recombinant ... incubation at 50ºC for h, and 77%, after incubation at 600C for h (Figure 4a) On the other hand, the residual xylanase activity after and h of incubation was 99%, at 500C, and 89%, at 600C, respectively...
... [63] and sulfated carbohydrates [64], and removing such glycans with PNGaseF evokes a hydrophobic shift analogous to that observed for dephosphorylated peptides [37] Following MS ⁄ MS analysis, former ... fluorophosphonates for monitoring serine hydrolases [73], and biotinylated a-bromobenzylphosphonates for detecting protein tyrosine phosphatases [74] ATP, ADP and AMP are important sensors for the energy ... Fund for Scientific Research ) Flanders (Belgium) The work in this paper was supported by research grants from the Fund for Scientific Research ) Flanders (Belgium) (project number G.0280.07) and...
... autoradiography, as outlined (C and D) Western blot analysis of Oct-1 (C) and Oct-2 (D) expression in whole cell protein (60 lgÆ lane)1) prepared from HEL (C and D; lane 1) and HEK293 (C and D; lane 2) cells ... the left and right of the (C) and (D), respectively, whilst the position of the Oct-1 (98 kDa approximately) and the two major forms of Oct-2 (75–80 and 55–60 kDa) detected in HEK293 and HEL cells, ... receptor gene expression tion and cells lysed and prepared for luciferase assays as described above HEK293 and HEL cell supernatants were assayed for both firefly and renilla luciferase activity...
... Most integrin a subunit genes lack both TATA and CCAAT boxes, except for the integrin a4 subunit gene which includes both TATA and CCAAT boxes andfor the integrin a6 subunit gene which contains ... universal primer for pBluescript SK(+); 5¢-CTT TATGTTTTTGGCGTCTTCC-3¢ (GL primer) and 5¢-CTAGCAAAATAGGCTGTCCC-3¢ (RV primer) for plasmids constructed in pGL3-basic Transfection and luciferase assay ... Ajinomoto, Tokyo, Japan) for h, and subsequently cultured for 48 h in RPMI-1640/10% fetal bovine serum After the cells were harvested, the cell extracts were assayed for luciferase activity with...
... other hand, for the case (ii) where parallel bonds reside in the polymer chain, the force exerted for each bond in parallel configuration is given by f = F/NB The probability to withstand the force ... interatomic potential in the form of Eq (4) consists of potential for backbone chain stretching and the potential for native contacts Go potential is a versatile model for protein modeling such ... (1 − δ j ,i +1 ) ⎢ ⎥ ⎣ ⎦ (4) Here, k1 and k2 are force constants for harmonic potential and quartic potential, respectively, ψ0 is the energy parameter for van der Waal’s potential, λ is the...
... (http://www.harvestplus.org) and by the Deutsche Forschungsgemeinschaft (DFG), Grant 892 ⁄ 1-3 We are indebted to J Mayer for valuable discussions We thank H Ernst for providing the synthetic substrates and E Scheffer for ... C7¢–C8¢ and C9–C10 ⁄ C5¢–C6¢ The formation of multiple dialdehyde products allows some conclusions to be drawn on the site preferences of OsCCD1 For instance, the C14, C17 and C19 dialdehydes formed ... °C, and at 68 °C, and 10 of final polymerization at 68 °C The obtained PCR product was purified using GFX PCR DNA and a Gel Band Purification Kit (Amersham Biosciences, Piscataway, NJ, USA), and...
... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal and C-terminal TAP-tagged ... Switzerland) and Markus Schirle, Manfred Raida, Anne-Marie Michon and Sonja Ghidelli (Cellzome AG, Heidelberg, Germany) for technical support, Rita Schmitz (Novartis, Basel, Switzerland) for providing ... expression vectors and USP7 cDNA as well as Shirley Gil-Parrado, Bruno Martoglio, Martin Renatus and Jorg Eder (Novartis, Basel, Switzerland) ¨ ´ for support and helpful discussions A Fernandez´ Montalvan...