pacific oyster crassostrea gigas identification of a critical period of vulnerability after spawning
Tài liệu Báo cáo khoa học: Identification of differentially expressed genes of the Pacific oyster Crassostrea gigas exposed to prolonged thermal stress docx
Ngày tải lên :
18/02/2014, 16:20
... ACCAGAAGACATTACAGTGAAAATTGA GGGAACCAGCAGTCGTCAAA CGTCCACTGAGAGGATGAGACA AAAGCAGAGCAGAAAAAGTGGAA GGACAATGCCGCGATCAG CAATGAACAAAAAAGTCGCAACA GGGATGGAGGGTAAGACCATACA ACGGAGGTTGACGGGACTT GCTGGCACCACGATTGG ... AATGCTGGCTCTCCCTCGAT GCTTGGCTACTGGACCATCAA GGAAATGGAAATAACAAGACAAATAGC GCGCAACTAATGCTTCCACAA TGACCAAGGCAACAGAACCA AATCAGACGGCCGGTATGTG CGAAAAAGGACAGCAGTTGAAA CTCATCCTCCACCGGATTGT CGTCCGATTTCTTCTCGTGTTT ACCAGAAGACATTACAGTGAAAATTGA ... to 3’) MTA-1 AATGCTGGCTCTCCCTCGAT GCTTGGCTACTGGACCATCAA GATACAGCAAACGGAAAGTCAACA CAGTTCCTCGGGCCAACA GCCCCCCTCCCACACA CATCTTCGGCCGTCTTTCC GTTCTTGTTCCTGCTCATCAGTATG TGGATCGCCAAAAACTCATG AATGCTGGCTCTCCCTCGAT...
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Báo cáo khoa học: Molecular identification and expression study of differentially regulated genes in the Pacific oyster Crassostrea gigas in response to pesticide exposure doc
Ngày tải lên :
30/03/2014, 15:20
... 5¢-ATCTGGAGAGCACATCATTGCTGGTGCA-3¢ 5¢-ACATCGAGGAAGAGTTTTCTATCCTGGA-3¢ 5¢-ACATCGCTGAGAATGTCAACGGGGATAT-3¢ 5¢-CTTTCTGGCCTCTCCAACATCCATGCCA-3¢ 5¢-ATGCCAAGGTAGTTTATGATGATCGAGA-3¢ 5¢-TCTCCTACGATCATCTCACCGTCACCGA-3¢ ... 5¢-GCACAGTTCCCCTACAGTCCCGCTTTAG-3¢ 5¢-CACGTCTCAGCAGGGAGAATAATCCCGA-3¢ 5¢-GAGCTCAGCGAGGACGGAAACCTCGCGT-3¢ ADI (up) 5¢-CCAATCAGGTAGGCCTTCATGGAGAGGA-3¢ 5¢-CCCAGAGATCCTCCAAGAGACAGCCAGT-3¢ ADI (up) ADI (down) ADI (down) 5¢-ATCTGGAGAGCACATCATTGCTGGTGCA-3¢ ... ADI and G (up) ADI (up) G (up) G (up) 5¢-GTGCATCAAAGAATTTTGGATAC-3¢ 5¢-AGAGAAGTGGCAGCTTTCGCTCAGTTTGG-3¢ 5¢-CTCGGCAAAGAAACCGCTGGTTCCTCCCA-3¢ 5¢-GAGACGTCCAGGAAATCTTCCGCAACACC-3¢ 5¢-CAGAGTGTGCACTAGCATGCGGTCCCGT-3¢...
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Báo cáo khoa học: Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions pot
Ngày tải lên :
16/03/2014, 23:20
... 5¢-AAACAGGCGGGAAACCACAGTAACACGGT-3¢ 5¢-CACTGGACGCTTTCATAACAAGGGGGCGT-3¢ 5¢-GATGACGTCCCCAGTCATGAGGGGTGGTC-3¢ 5¢-TGGGGGATGGAGGGTAAGACCATACACTT-3¢ 5¢-TTCTATAACGGAACATTATACCAACAAGG-3¢ 5¢-CAACATTTACCTGGGGCAGGTGGGTTCAG-3¢ ... 5¢-CAACATTTACCTGGGGCAGGTGGGTTCAG-3¢ 5¢-AATCCAAAAGTGCAGGCCTCACTAGCAGC-3¢ 5¢-TTGCCGACTAATTCCGGGACTCCATCATC-3¢ 5¢-CCGTCTTGCCAGAGTTTCTCCACCTCCTC-3¢ 5¢-GTCGTCAACAACGATCCTGACGTTGGGGA-3¢ 5¢-TACTGTCTTCTGCTAAACGCCAC-3¢ 5¢-GTCGTGATATTGAGGTGCCAGCC-3¢ ... 5¢-GTCGTGATATTGAGGTGCCAGCC-3¢ 5¢-GCCCAGACGGGAAAATGCGTGTG-3¢ 5¢-CAGTTACACGATGCTTTGGCGCA-3¢ 5¢-GGAATAGATCTTGGAACCACATA-3¢ 5¢-TTGCCAAGATATGCTTCTGCAGT-3¢ BTF3 Glycogen phosphorylase Delta-9 desaturase Putative...
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Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
Ngày tải lên :
08/03/2014, 16:20
... Germany) was used The AminoQuant amino-acid analyzer combines the OPA and FMOC as derivatization reagents for the complete detection of all residues BSA was used as a standard protein to calculate ... result was the identification of an amino-acid position in the PXR structure that dramatically affects the basal activity of both the human and mouse receptors MATERIALS AND METHODS Plasmid constructs, ... like to thank Kristina Zachrisson for performing the amino˚ ´ acid analysis and Sven-Ake Franzen, Andrea Varadi and Marianne Israelsson for DNA sequence analysis 16 REFERENCES Kliewer, S .A. , Moore,...
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Báo cáo khoa học: Proteomic identification of all plastid-specific ribosomal proteins in higher plant chloroplast 30S ribosomal subunit PSRP-2 (U1A-type domains), PSRP-3a/b (ycf65 homologue) and PSRP-4 (Thx homologue) doc
Ngày tải lên :
17/03/2014, 09:20
... 5¢-GGAATTCGTCGACGCGTTAA AAAAGATAGCAGCATTGACAC-3¢; P3-1, 5¢-ATGG GIAAYGARGTIGAYATHG-3¢; P3-2, 5¢-CTAGACC TATGTTTTTCTCCATCC-3¢; P4-1, 5¢-CCIAARAAYA ARAAYAARGG-3¢; P4-2, 5¢-CAGATAGGAAGAGGG GCAAGGA-3¢; P4-3, 5¢-GGAATTCTAGATATCGTC ... 5¢-CG GGATCCCAACTGGTAATGGTAGCGACCGGC-3¢; P2-1, 5¢-ATGGAYATHGCIACIACICARGC-3¢; P2-2, 5¢-TAGCAACTCATTCGTCACTGTC-3¢; P2-3, 5¢-GGA ATTCTAGATATCGTCGACAATTTGTGTTACTACC AAAATC-3¢; P2-4, 5¢-GGAATTCGTCGACGCGTTAA ... 5¢-GGAATTCTAGATATCGTC GACTTATCTTCAGAACTTGTTGC-3¢; P4-4, 5¢-GGA ATTCGTCGACGCGTTTTTCAACAAATCATCATAT A- 3¢; TAG1, 5¢-GGAATTCTAGATATCGTCG-3¢; TAG2, 5¢-GGAATTCGTCGACGCG-3¢ Computer analysis A homology search...
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Báo cáo khoa học: Identification of a copper-repressible C-type heme protein of Methylococcus capsulatus (Bath) A member of a novel group of the bacterial di-heme cytochrome c peroxidase family of proteins docx
Ngày tải lên :
23/03/2014, 11:20
... 2589 R sapE–F 2590 F sapE–R 2590 R mopB–F 3103 F mopB–R 3103 R GGCAACGAGCAAGGTCCGAAG AAGTCGTTGCAATCGGCGTCG GGCAACGAGCAAGGTCCGAAG GACGTCGTGAGTGCCTCCGTG CGACGTGCAGTATTACTTTTCTAGGG AGTATCAAACCGTGCTGGTCTCC ... processing would lead to a mature protein of 732 amino acids with a theoretical molecular mass of 78 kDa A search in the PROSITE database of protein families and domains [12] with MCA2590 revealed two ... immunoblot analyses (B) of proteins obtained during the fractionation of M capsulatus Samples of each step during the fractionation procedure were collected and comparable samples (10 lL of each mL fraction)...
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Báo cáo khoa học: Identification of a domain in the a-subunit of the oxaloacetate decarboxylase Na+ pump that accomplishes complex formation with the c-subunit pot
Ngày tải lên :
23/03/2014, 13:20
... E71, H81 and H83 of c-2 (indicated by arrows) 847 Association domain of oxaloacetate decarboxylase 10 Fig Dissociation and reassociation of c¢-2 and a- 2 The dissociation of the proteins that were ... a- 2 or the number of remaining amino acids of a- 2-C FEBS Journal 272 (2005) 846–855 ª 2005 FEBS 849 Association domain of oxaloacetate decarboxylase A P Dahinden et al A B B Fig Complex formation ... supernatant was subjected to ultracentrifugation (1 h at 200 000 g) to separate the cytosolic fraction and the membrane fraction Purification VcOadA-2, VcOadA-2-C, and VcOadG A- 2 and its derivatives...
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Báo cáo khoa học: "Identification of Domain-Specific Senses in a Machine-Readable Dictionary" potx
Ngày tải lên :
30/03/2014, 21:20
... Magnini and G Cavaglia 2000 Integrating Subject Field Codes into WordNet In In Proc of LREC-2000 Y Matsumoto, A Kitauchi, T Yamashita, Y Hirano, Y Matsuda, K Takaoka, and M Asahara 2000 Japanese Morphological ... presented a method for assigning categories to each sense of words in a machine-readable dictionary For evaluation of the method using WordNet 3.0, the average precision was 0.661, and recall against ... Sd | (1) We applied the algorithm for each domain We note that the matrix M is a high-dimensional space Therefore, we used a ScaLAPACK, a library of high-performance linear algebra routines for...
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Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
Ngày tải lên :
15/02/2014, 01:20
... ACA G) and EWS reverse d(CGC TCG AGT CAC TAG TAG GGC CGA TCT CTG C), for pGEX–EWS; EAD forward d(CGG AAT TCA TGG CGT CCA CGG ATT ACA G) and EAD reverse d(CGC TCG AGT CAT CCG GAA AAT CCT CCA GAC ... pGEX–EAD; RGG1 forward d(CGG AAT TCC CAG GAG AGA ACC GGA GCA T) and RGG1 reverse d(CGC TCG AGT CAA TCA AGA TCT GGT CCT TCA TCC ATG G), for pGEX–RGG1; RRM– RGG2–ZnF forward d(CGG AAT TCC TAG GCC CAC ... protein arginine methyltransferase and toward the Ewing sarcoma protein and a peptide Proteins 61, 164–175 48 Raman B, Guarnaccia C, Nadassy K, Zakhariev S, Pintar A, Zanuttin F, Frigyes D, Acatrinei...
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Tài liệu Báo cáo khoa học: The cellulosomes from Clostridium cellulolyticum Identification of new components and synergies between complexes ppt
Ngày tải lên :
18/02/2014, 08:20
... present in each fraction were analysed Protein analyses of the fractionated cellulosomes The six fractions, separated as described above, were first analysed using Native PAGE In all fractions, a single ... procedures as each of the fractions analysed separately Each of the fractions obtained by anion-exchange chromatography showed numerous proteins, most of which had molecular masses in the range 30–160 ... all fractions showed a substantial level of activity that was never less than half that of the most active fraction, F1 On Avicel, F5 was the most efficient fraction, and the least active fractions,...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
Ngày tải lên :
18/02/2014, 14:20
... TCA ACA GAG GTG GGC AGC CTT GTA GTG GTG TCA GTG TGA ATG ATC CAC TCA TCA CAT GAG AAT TTG TTA AGC TGG ACA TAG ATC CAT CAG TTG TCA TGA TCA GGT CTT CTC ATG TGA TCA ACA GTG GTC TCA CGG TTG TGG ACA ATG ... TCA TGA CTC GGA GAA GAA GGA GAG CAA CTG CAA ATA GAA GAA TTG TGG GAA GGA GGA GAG TTG TGA CCG GGT GCG ATC TCA CAT GAG TCC TTC TTC TCC CTC TCC CAC GCC ATC TTC TTC CCA TGT TTC TCC TCC CTC ATG TGA ATG ... TTG TGG ACA ATG CTA CAA CCA TGC TAT CAA TCG AAG TCA CAT TAC ACA GGT ATA TGA GCT AAG CTG TTG CTG GCA CTT CCA TGT GAT TTG CCA CTC ATT ATG CGA ATG (lpxX sense) (lpxX antisense) GTG CAG TCG (lpxX sense;...
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Tài liệu Báo cáo khoa học: Suppressed catalytic efficiency of plasmin in the presence of long-chain fatty acids Identification of kinetic parameters from continuous enzymatic assay with Monte Carlo simulation ppt
Ngày tải lên :
18/02/2014, 17:20
... all three fatty acids affected the activity of miniplasmin in the same manner as that of plasmin; apparent activation was seen with stearate and inhibition was seen with oleate and arachidonate ... set of parameters in the absence of fatty acids Shaded ellipses combine parameters belonging to the same type of fatty acid (data for oleate and the absence of fatty acids are found in the unshaded ... justify the application of Model III as being the most adequate for the final evaluation of the kinetic parameters in the presence of stearate Evaluation of kinetic parameters Fig Comparison of three...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
Ngày tải lên :
18/02/2014, 17:20
... TCATCAT-3¢; rOMM-64-IV, 5¢-CGCGGATCCGCCCCT GTTAATGATGGAACC-3¢ and 5¢-CGCCTCGAGCTAA GAAGACTGGGCTGCCAG-3¢; rOMM-64-V, 5¢-CGCGG ATCCAGGCAAGATTTTAAGCATCCA-3¢ and 5¢-CGCC TCCACCTAAGAGGCATCCTTGTCCAC-3¢; ... 5¢-CGCCTCCA CCTAAGAGGCATCCTTGTCCAC-3¢; rOMM-64-II, 5¢CGCGGATCCACCGTAGACACTTATGATATA-3¢ and 5¢-CGCCTCGAGCTAAGAGTCAGCTTGCACGTC-3¢; rOMM-64-III, 5¢-CGCGGATCCGCTGATGTGACCAGT GATGAC-3¢ and 5¢-CGCCTCGAGCTATTTGGGCTCTT ... digestion of genomic DNA contamination in the total RNA was confirmed by lack of amplification of a b-actin mRNA fragment by PCR using a pair of primers (5¢-ATCACCATCGGCAACGAGAG-3¢ and 5¢-TGGAGTTGTAGGTGGTCTCGTG-3¢)...
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Tài liệu Báo cáo khoa học: Direct identification of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS docx
Ngày tải lên :
19/02/2014, 02:20
... identification of an HFB1-like protein, which, after N-terminal processing (MKFFTAAALFAAVAIA), C-terminal processing (AVGA) and disulfide bond formation, has a mass of 7743 Da T longibrachiatum The main ... Trichoderma hydrophobins T Neuhof et al Fig Intact-cell MALDI-TOF spectra of mycelia (A, C, E) and sporulating mycelia (B, D, F) of three strains of H jecorina grown on malt agar The masses displayed ... MQFSIVALFATGALA site and the C-terminal Phe, respectively showed a characteristic set of mass peaks in the range of 5–10 kDa, typically including two dominating peaks at approximately m ⁄ z 7000 As...
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Tài liệu Báo cáo khoa học: Identification of b-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay docx
Ngày tải lên :
19/02/2014, 07:20
... and a final extension reaction for 10 at 72 °C) using Ex Taq DNA polymerase (Takara Bio Inc, Shiga, Japan) with template (the cDNA pool described above) and primers (5¢-AAACACTAGTATGCTAGACATCAAAGG ... Banno N, Akihisa T, Tokuda H, Yasukawa K, Higashihara H, Ukiya M, Watanabe K, Kimura Y, Hasegawa J & Nishino H (2004) Triterpene acids from the leaves of Perilla frutescens and their anti-inflammatory ... Shibuya et al 34 Kitagawa I, Yoshikawa M, Wang HK, Saito M, Tosirisuk V, Fujiwara T & Tomita K (1982) Revised structures of soyasapogenol A, soyasapogenol B, and soyasapogenol E, oleanene sapogenols...
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Tài liệu Báo cáo khoa học: Identification of GAS-dependent interferon-sensitive target genes whose transcription is STAT2-dependent but ISGF3-independent doc
Ngày tải lên :
19/02/2014, 07:20
... Qiagen RNeasy mini-kit according to the manufacturer’s protocol The preparation of cDNAs, sample hybridization and scanning of HG-U-133 A GeneChipÒ Arrays (Affymetrix, Santa Clara, CA, USA) was ... pathwayassist software (Iobion Informatics LLC, Stratagene, La Jolla, CA, USA) and the Online Predicted Human Interaction Database (OPHID; http://ophid.utoronto.ca) OPHID is a webbased database ... IFN-stimulated U 6A, U 6A- 2 and VV-II cells using relative quantitative real-time PCR as for Fig For each sample, b-actin was evaluated as a reference gene and used for normalization For each gene, data...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
Ngày tải lên :
19/02/2014, 07:20
... at iGENE (Tsukuba, Japan) The forward sequences of the siRNAs were: #973, sense 5¢-UCAUCACACUGAUAACCAACACUGA-AG-3¢; #2578, sense 5¢-GGAUCAAAGAGAACACUGGGGUAUA-AG-3¢; and #1513 sense, 5¢-AGUUCACGUGCAAGAACAAG ... 5¢-AGUUCACGUGCAAGAACAAG UUCUG-AG-3¢ The forward sequence of the scrambled RNA was 5¢-GAUCCAAGUAAUACAGAGAUGGGAG AG-3¢ OVISE cells were plated the day before transfection at a cell density of 50–60% saturation ... matriptase as a major component and a few contaminating proteins as analyzed by SDS ⁄ PAGE RNAi experiments with OVISE cells Matriptase siRNAs and a scrambled RNA as a control were designed and...
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Tài liệu Báo cáo khoa học: Bioinformatics of the glycoside hydrolase family 57 and identification of catalytic residues in amylopullulanase from Thermococcus hydrothermalis doc
Ngày tải lên :
19/02/2014, 12:20
... CAD85943.1 AAQ66699.1 AAL64906.1 AAL63225.1 AAL64927.1 CAB49868.1 CAB49676.1 CAB49100.1 CAB49104.1 AAG28455.1 AAL80994.1 AAL81517.1 AAA72035.1 AAL80396.1 AAB71229.1 AAL82059.1 BAA29442.1 BAA30492.1 ... BAA30492.1 BAA30120.1 BAA29262.1 BAA22062.1 CAD72460.1 AAN56266.1 AAK41260.1 AAK41420.1 BAB65830.1 BAB66135.1 BAC09526.1 BAC08829.1 BAC09822.1 BAA18743.1 BAA10043.1 AAM25110.1 AAD28552.1 BAA22063.1 BAC10983.1 ... Q9HL91_THEAC BAB74149.1 BAB73267.1 BAB72585.1 AAC06900.1 BAB05134.1 AAO79410.1 AAK80369.1 CAA30735.1 BAC89267.1 AAB99631.1 AAM07401.1 AAM07400.1 AAM30557.1 AAM30558.1 CAC30667.1 AAK47445.1 CAD85942.1...
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Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identification of oscillophores ppt
Ngày tải lên :
19/02/2014, 16:20
... than negative combined autoinfluence paths occur in that subnetwork We shall speak of a negative subgraph in this case We define the value of a subgraph that contains p reactions and p concentrations, ... degradation (–ajsajs) and a second influence through its autocatalytic feedback (+ajsbjs) through the same reaction, vs The latter influences cancel each other, again as all stoichiometries are ... oscillations can be obtained Analysis of negative and positive subgraphs and comparison of their values gives rise to the estimation of the kinetic parameters that enable such oscillations The main...
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Tài liệu Báo cáo khoa học: Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae docx
Ngày tải lên :
20/02/2014, 01:20
... yef1-attB1FSD AAAAAGCAGGCTCCGAAGGAGATATAAAA ATGAAAACTGATAGATTACTG AGAAAGCTGGGTGGATTGCAAAATGAGCCTGAC ACAAGTTTGTACAAAAAAGCAGGCT ACCACTTTGTACAAGAAAGCTGGGT CAATAAATCTGCTTACGTGACATTTTTTACTAAAAGAGAAT ATGCGTACGCTGCAGGTCGAC ... TTAATCGATGAATTCGAGCTCG CATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAA ATGCCAATTCTGTGTTTCCCGGAAATG CTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGT TTAGTAAAGTTCGTTTGCCGATACATG CGTTATGAAAATCACTATTATCCCC AAAAGCTTAGATTGCAAAATGAGCCTGACGA ... ATGCGTACGCTGCAGGTCGAC GAACCCTTGACTACGGAAACGCAGGATGTGGGAAATCG TTAATCGATGAATTCGAGCTCG CATAAATAAAAGGATAAAAAGGTTAAGGATACTGATTAAA ATGCGTACGCTGCAGGTCGAC CTTAGAGAATCTCATTGAATCTTTGCATTCAGAGCGT TTAATCGATGAATTCGAGCTCG...
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