oxidative stress indices in parkinsons disease biochemical determination
Báo cáo khoa học: Yeast oxidative stress response Influences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot
Ngày tải lên :
16/03/2014, 14:20
... carriers are involved in oxidative
stress during heat stress in Saccharomyces cerevisiae.
Mol Cel Biol 21, 8483–8489.
17 Guo J & Lemire BD (2003) The ubiquinone-binding site
of the Saccharomyces ... in Saccharomyces cerevisiae:
insights into ageing, apoptosis and diseases. Mol Aspects
Med 22, 217–246.
10 Moye-Rowley WS (2002) Transcription factors regula-
ting the response to oxidative stress ... showing that Yap1 binding activity may be
affected. It was shown that the accessibility of Yap1
to the GSH1 promoter could be repressed by Cbf1, a
DNA-binding protein that binds to elements in...
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Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"
Ngày tải lên :
26/10/2012, 09:07
... synthase. The increased
activities of argininosuccinate synthetase and argininosuccinate lyase suggest the increased
and effective recycling of citrulline to arginine in anoxia, making nitric ... in brain in anoxia as observed by in-
crease in NOx concentration in this study support the
earlier findings of NO involvement in pathophysiol-
ogy of hypobaric hypoxia in brain (42-43). In this ... arginine at the site of synthesis.
Arginine is a semi essential amino acid and in CNS, its
availability depends upon: (a) uptake from the circu-
lating arginine and (b) recycling of citrulline...
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Tài liệu Báo cáo khoa học: Metabolic gene switching in the murine female heart parallels enhanced mitochondrial respiratory function in response to oxidative stress pdf
Ngày tải lên :
18/02/2014, 16:20
... Young
ME, Pham M, Zhang D, Cooksey RC, McClain DA,
Litwin SE et al. (2002) Insulin signaling coordinately
M. F. Essop et al. Metabolic gene switching in murine female heart
FEBS Journal 274 (2007) ... additional insights into this
interesting question.
Conclusions
In summary, we have identified a novel metabolic
gene switch (decrease in fatty acid utilization,
increase in glucose utilization) in the ... 2007)
doi:10.1111/j.1742-4658.2007.06051.x
The mechanisms underlying increased cardioprotection in younger female
mice are unclear. We hypothesized that serine-threonine protein kinase
(protein kinase B; Akt) triggers a metabolic...
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Tài liệu Báo cáo khoa học: Oxidative stress in the hippocampus after pilocarpineinduced status epilepticus in Wistar rats doc
Ngày tải lên :
19/02/2014, 16:20
... oxidative stress.
Our findings show that pilocarpine induces SE,
which can produce alterations in superoxide dismutase
and catalase activities in different areas, thereby pro-
tecting the brain ... [2,19,20], immediately
after pilocarpine administration, animals persistently
show behavioral changes, including initial akinesia,
ataxic lurching, peripheral cholinergic signs (miosis,
piloerection, ... 10 min. A
550
was meas-
ured using a microplate reader. Nitrite concentration was
determined from a standard nitrite curve generated using
NaNO
2
.
Determination of GSH
GSH in the pilocarpine...
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Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx
Ngày tải lên :
21/02/2014, 03:20
... treatment, cells were incubated in medium
containing 50 lgặmL
)1
neutral red at 37 C for 3 h, then
xed with 1% formaldehyde containing 1% CaCl
2
for
1 min. The dye incorporated into viable cells ... Hsp105a
plays an important role in protection of neuronal cells
against stress- induced apoptosis [26]. In accordance with
these findings, ischemia/reperfusion in the rat forebrain
induces the expression ... mitogen-activated protein kinase
(p38). Furthermore, oxidative stress- induced apoptosis was
suppressed by SB202190, a potent inhibitor of p38, in F9
cells. These findings indicated that the activation...
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Ambient particulate air pollution induces oxidative stress and alterations of mitochondria and gene expression in brown and white adipose tissues ppt
Ngày tải lên :
06/03/2014, 19:20
... PM
2.5
onWATandBATspecificgenesin
different adipose depots. There are several major find-
ings in this study. First, exposure to PM
2.5
resulted in
oxidative stress in BAT. Second, exposure to PM
2.5
induced changes ... 8 of 14
inflammatory response and IR development, were exam-
ined in mice. As shown in Figure 3, PM
2.5
exposure
induced a marked increase in macroph age (F4/80
+
cells)
infiltration in eWAT. ... reduced in both
eWAT and iBAT in response to PM
2.5
exposure.
Recent studies have implicated PM
2.5
in in creased adi-
pose inflammation and insulin resistance [11,12], and
epidemiological studies indicate...
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Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
Ngày tải lên :
07/03/2014, 12:20
... neuron. The turning point upon which
the cell looses the ability to buffer the incoming calcium
resulting in an abrupt, sustained and irreversible
increase in [Ca
2+
]
c
, probably coincides with ... functions, in addition to
having supporting functions for oxidative defences [79],
involving the thioredoxin system [40]. Aconitase acts
also as an iron-responsive element binding protein, iso-
citrate ... by proteins resi-
ding in both the inner and outer mitochondrial
membrane, that is activated by mitochondrial Ca
2+
overloading and other factors including oxidative stress
[88,89]. In neurons...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
Ngày tải lên :
07/03/2014, 12:20
... enzymatic activity
involved in the oxidative stress in S. solfataricus.
To detect differences in the response to various
agents we induced oxidative stress with paraquat,
an O
2
ã
)
generating compound, ... stress conditions, are important in deter-
mining the level of Bcp2 protein. Similar results are
observed for genes and related proteins involved in
oxidative stress in other microrganisms [24]. ... S
p
peroxidatic cysteine;
X unidentified electron donor; S
R
resolving
cysteine; RSH protein or domain with CXXC
motif (e.g. thioredoxin). In typical 2-Cys, S
p
at the N terminus and S
R
at the C terminus
belong...
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Báo cáo khoa học: Cotton GhMPK2 is involved in multiple signaling pathways and mediates defense responses to pathogen infection and oxidative stress doc
Ngày tải lên :
14/03/2014, 23:20
... protein kinase and wound-
induced protein kinase, two MAPKs in group A, were
activated by inoculation with the tobacco mosaic virus
(TMV) [10]. Their orthologs in other plant species,
including ... accumulation in wild-type and transgenic
plants in response to abiotic and abiotic stresses. (A) Virus infec-
tion-induced H
2
O
2
accumulation detected by DAB staining. (B) Abi-
otic stress- induced ... MPK6 in Arabidopsis (group A
MAPKs), SIMK and SAMK in alfalfa, and
LeMPK1 ⁄ 2(Lycopersicon esculentum SA-induced pro-
tein kinase) and LeMPK3 (L. esculentum wound-
induced protein kinase) in tomato,...
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Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf
Ngày tải lên :
15/03/2014, 23:20
... indicating the presence of two catalytically
independent domains, the N-terminal domain, display-
ing ALDH activity, and the C-terminal domain, con-
taining an iron-binding domain, which is involved ... against oxidative stress
damage, have not been reported.
In vivo effects on glucose-fermenting enzymes
and fluxes under oxidative stress
Recently, we reported that amebas incubated for
30 min in ... bind
close to the Fe–S clusters, blocking the access of ROS.
In this regard, a preliminary docking analysis with
coenzymes in the 3D model of EhPFOR suggested that
the CoA-binding site was, indeed,...
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Báo cáo khoa học: Exogenous oxidative stress induces Ca2+ release in the yeast Saccharomyces cerevisiae potx
Ngày tải lên :
15/03/2014, 23:20
... gene encoding
the regulatory subunit of calcineurin) under oxidative
stress was tested. It was noted that CNB1 deletion
resulted in an increase of tolerance to H
2
O
2
(Fig. 3B),
Oxidative stress ... cells were resuspended in 0.1 m Mes ⁄ Tris
buffer (pH 6.5) and transferred to the luminometer tube. A
cellular luminescence baseline was determined by 1 min of
recording at 1 s intervals. The exogenous ... factors regulat-
ing the response to oxidative stress in yeast. Antioxid
Redox Signal 4, 123–140.
25 Ikner A & Shiozaki K (2005) Yeast signaling pathways
in the oxidative stress response....
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Báo cáo khoa học: Fasting-induced oxidative stress in very long chain acyl-CoA dehydrogenase-deficient mice pdf
Ngày tải lên :
15/03/2014, 23:20
... mice. Fasting induced a significant increase in both
genotypes; however, this was more evident in the
VLCAD KO mice (Fig. 3A). Interestingly, the MCT
diet also induced AOX gene expression in WT mice.
After ... observed in human VLCADD [5]. In fact, in
both mice and humans, clinical symptoms become
mainly evident as a consequence of triggers such
as fasting, resulting in the accumulation of long-chain
acylcarnitines, ... quantitation of microgram quantities of protein
utilizing the principle of protein-dye binding. Anal
Biochem 72, 248–254.
S. Tucci et al. Oxidative stress in VLCAD KO mice
FEBS Journal 277 (2010)...
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Báo cáo khoa học: Oxidative stress induces a reversible flux of cysteine from tissues to blood in vivo in the rat pptx
Ngày tải lên :
16/03/2014, 02:20
... ROS, as indicated by the lack of increase
in lipid peroxidation observed in in vitro and in vivo
experiments [21]. In previous studies [22,23], we
observed that a single intraperitoneal administration ... the maximum increase of cysteine
induced by diamide. Values indicate the increase of total Cys over
levels at time zero in rat blood 75 min after the start of diamide
infusion (60 min infusion, ... did not induce evident decreases in cyste-
ine and GSH levels in the organs analyzed.
Together, these findings prompted us to further
investigate the origin of cysteine that enters blood.
In theory,...
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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc
Ngày tải lên :
16/03/2014, 22:20
... 5. In uence of Ca
2+
increase in combination with H ⁄ Rand
extramitochondrial NADH on respiration of brain mitochondria. Rat
brain mitochondria (0.5 mgặmL
)1
) were incubated at 30 °C in incu-
bation ... brain mitochon-
dria by H ⁄ R and Ca
2+
. Brain mitochondria ( 0.5 mg proteinặmL
)1
)
were incubated at 30 C under the conditions indicated: Ca
2+
,
15 min in incubation medium; Ca
2+
, 15 min ... sug-
gests that the mitochondrial injury is caused by open-
ing of the mPTP. There is evidence that ADP inhibits
opening of the mPTP by occupying binding sites
located in the inner and outer mitochondrial...
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Báo cáo khoa học: Role of Acinetobacter calcoaceticus 3,4-dihydrocoumarin hydrolase in oxidative stress defence against peroxoacids Kohsuke Honda, Michihiko Kataoka, Eiji Sakuradani and Sakayu Shimizu doc
Ngày tải lên :
17/03/2014, 09:20
... contains the gene
encoding DCH lacking the C-terminal region, in which
Asp and His residues involved in the catalytic triad are
contained. The kanamycin resistance gene (Km
R
), including
its own promoter, ... 270) Ó FEBS 2003
Oxidative stress sensitivity assays
To test the susceptibility of A. calcoaceticus strains and the
recombinant E. coli strains to oxidative stress agents, disk
inhibition assays ... expected to result from insertion of a
single copy of the Km
R
gene.
3,4-Dihydrocoumarin-hydrolysing activity, monochloro-
dimedon-brominating activity, and peracetic acid-degrading
activity were compared...
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