... with η the < /b> viscosity of < /b> the < /b> solvent, k Boltzman’s constant, and Tthe < /b> absolute temperature For polydisperse samples a distribution of < /b> the < /b> hydrodynamic radii can be obtained in this way from the < /b> ... between the < /b> two interpenetrating networks may be a useful property that can be exploited in product development Objectives The < /b> objective of < /b> the < /b> present investigation was to study the < /b> influence of < /b> aggregation ... different concentrations of < /b> κ-car The < /b> solid line indicates the < /b> time dependence of < /b> the < /b> temperature The < /b> dashed lines indicate the < /b> position of < /b> the < /b> temperature where the < /b> coil helix transition occurs (b) ...
... with η the < /b> viscosity of < /b> the < /b> solvent, k Boltzman’s constant, and Tthe < /b> absolute temperature For polydisperse samples a distribution of < /b> the < /b> hydrodynamic radii can be obtained in this way from the < /b> ... between the < /b> two interpenetrating networks may be a useful property that can be exploited in product development Objectives The < /b> objective of < /b> the < /b> present investigation was to study the < /b> influence of < /b> aggregation ... different concentrations of < /b> κ-car The < /b> solid line indicates the < /b> time dependence of < /b> the < /b> temperature The < /b> dashed lines indicate the < /b> position of < /b> the < /b> temperature where the < /b> coil helix transition occurs (b) ...
... inhibited by recombinant HBs but not by the < /b> recombinant LHBs The < /b> binding of < /b> SHBs with human hepatocytes was further supported by the < /b> observation from Bruin et al [39] They have found that the < /b> particles ... in the < /b> nucleus after two days of < /b> the < /b> post inoculation, this is agreement with the < /b> observation from DHBV [69] The < /b> result from the < /b> kinetic study on the < /b> internalization and transportation of < /b> HBV by ... demonstrated that the < /b> uptake of < /b> DHBV by primary duck hepatocyte (PDH) requires endocytosis [66] The < /b> deficient mutant preventing the < /b> transport of < /b> DHBV to endosome has abolished the < /b> infection However,...
... signaling pathways The < /b> results were quite different between T- pIC treatment and M-pIC treatment First, in T- pIC treatment, the < /b> results showed that NS3-4As (the < /b> 1B- 1 and HCV-O strains of < /b> genotype 1b) could ... not in the < /b> PH5CH8 cells transfected with GL2 or TLR4 siRNA (Fig 3B) This result suggests that the < /b> activation of < /b> IRF-3 by M-pIC treatment is mediated by the < /b> TLR3 ⁄ TRIF signaling pathway We obtained ... activated in M-pIC treatment, and to determine if its activation is mediated by the < /b> TLR3 but not the < /b> TLR4 signaling pathway, we examined whether or not activation of < /b> IRF-3 by M-pIC treatment is specifically...
... defined by the < /b> promoters of < /b> the < /b> two reporter genes (3¢ to one of < /b> them, Fig 4, bottom), the < /b> PTS is able to overcome the < /b> enhancer blocking effect of < /b> the < /b> boundary, and restricts the < /b> enhancer activity to ... between the < /b> Abd -B promoter and the < /b> iab regions [13] The < /b> unusual properties of < /b> the < /b> tmr led to the < /b> hypothesis that it may be involved in a process that normally targets the < /b> iab regions to the < /b> Abd -B promoter ... near the < /b> promoter [13] Deletion of < /b> these sequences together with the < /b> promoter appears to free the < /b> enhancers from a bond that tethers them to the < /b> Abd -B gene in cis, and allows them to regulate the...
... can be seen concomitant with the < /b> reduced state at 1515 cm)1 and the < /b> mode typical for the < /b> deprotonated form < /b> at 1498 cm)1, indicating the < /b> protonation of < /b> a tyrosine residue with the < /b> reduction of < /b> the < /b> ... respectively) The < /b> potential at )220 mV can be attributed to the < /b> heme a3–CN– signal, this shift reflecting the < /b> characteristic behavior of < /b> cytochrome c oxidases heme a is expected to contribute with two steps, ... of < /b> the < /b> data presented here, in spite of < /b> the < /b> fact that bands in the < /b> difference spectra are observed in the < /b> region where the < /b> modes were attributed The < /b> vibrational modes of < /b> bound cyanide Electrochemically...
... ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCA TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGA TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAA GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTG CACGTTAATCAATTGACTAAGTGAACTGTTCTTGCTTC Table Yeast strains used in this ... the < /b> TRP sequence is positioned very close to the < /b> ATP-binding pocket in the < /b> adjacent subunit Thus the < /b> TRP sequence at the < /b> beginning of < /b> the < /b> C-terminal helix and the < /b> RDF sequence at the < /b> end of < /b> the < /b> ... First, the < /b> steady-state expression level of < /b> each Vps4p mutant protein tested is equivalent to that of < /b> wild-type Vps4p Second, each of < /b> the < /b> mutant proteins tested retained the < /b> ability to interact...
... Findings that cytokinin-O-glucosides are natural substrates for both of < /b> the < /b> two b- glucosidases, Zm-p60.1 and Bgl4:1, but the < /b> architecture of < /b> their sites that recognize the < /b> aglycone moieties of < /b> these ... the < /b> enzyme’s relative catalytic efficiency, defined as (kcat ⁄ Km)mutant ⁄ (kcat ⁄ Km)WT by 20% compared with the < /b> wild-type for both substrates, by increasing kcat By contrast, the < /b> F193A, F20 0K ... conferred by the < /b> W37 3K mutation indicate a previously unrecognized function of < /b> W373 in the < /b> determination of < /b> the < /b> catalytic rate of < /b> the < /b> enzyme, albeit one that is consistent with the < /b> involvement of < /b> F193–aglycone–W373...
... preferentially binds to the < /b> unmethylated CENP -B box DNA rather than the < /b> methylated form < /b> Using the < /b> complex-reconstitution assay, we tested the < /b> binding of < /b> CENP -B( 1–129) to the < /b> unmethylated and methylated ... CENP -B and the < /b> CpG-methylated CENP -B box In eukaryotes, DNA methylation occurs at the < /b> C5 atom of < /b> cytosine by the < /b> action of < /b> methyltransferases To evaluate the < /b> effect of < /b> CpG methylation on the < /b> CENP -B DNA ... E) These results indicate that CENP -B preferentially forms a complex with the < /b> unmethylated CENP -B box DNA, rather than the < /b> methylated CENP -B box DNA Y Tanaka et al The < /b> CENP -B binding affinity to...
... At first, the < /b> bucket starts to spin but the < /b> water ins~de remains fairly stationary; the < /b> surface of < /b> the < /b> stationary water stays nice and flat As the < /b> bucket picks u p speed, little by little its ... each other < /b> out, as the < /b> peak tries to make the < /b> water go up while the < /b> trough tries to drag it down If the < /b> height of < /b> one wave's peak equals the < /b> depth of < /b> the < /b> other'< /b> s trough, there will be perfect cancellation ... bucket expenment in the < /b> following may At the < /b> beginning of < /b> the < /b> experiment, the < /b> bucket is spinning with respect to absolute space, but the < /b> water is stationary v+rith respect to absolute space That's...
... Brilliant Blue staining Acknowledgements The < /b> authors would like to thank Professor Siddhartha Roy, Indian Institute of < /b> Chemical Biology, Kolkata, for the < /b> gift of < /b> the < /b> E coli strain NUT-21 containing ... enabled by the < /b> binding of < /b> its central helical region to the < /b> substrate-entry cleft of < /b> H B [22], as pointed out previously [11] This probably makes CIII a general inhibitor for the < /b> cytosolic substrates ... the < /b> interactions of < /b> the < /b> DnaK–DnaJ–GrpE machinery and by H B- mediated degradation Interestingly, these latter proteins that promote the < /b> degradation of < /b> r32 are themselves produced as a result of...
... coactivators was more important to their ability to synergize with other < /b> transcription factors than to activate transcription by themselves Taken together, these data suggest that in the < /b> activation of < /b> ... 4) These interactions were rather weak but could be important in the < /b> context of < /b> the < /b> IFN -b promoter if the < /b> arrangement of < /b> the < /b> PRDs allows them to occur The < /b> importance of < /b> these interactions was tested ... elements in the < /b> promoter and to the < /b> nature of < /b> the < /b> factors they bind To investigate the < /b> effect of < /b> promoter context on the < /b> transcriptional activity of < /b> IRFs, we used undifferentiated P19 cells and two...
... This is not meant to imply that a peptide that promotes negative curvature will cause the < /b> formation of < /b> nonlamellar structures, but rather that it will facilitate the < /b> formation of < /b> transient structures ... phospholipid, DPam2PtdEtn, to obtain an indication of < /b> the < /b> relationship between insertion of < /b> the < /b> peptide and the < /b> stability of < /b> the < /b> bilayer phase DPam2PtdEtn is a zwitterionic lipid with a bilayer to hexagonal ... negative curvature is related to the < /b> lytic action of < /b> mastoparan is not well understood, except for the < /b> fact that it would tend to destabilize the < /b> bilayer phase toward the < /b> formation of < /b> inverted phase...
... reliable estimate of < /b> the < /b> amount of < /b> newly produced B and T lymphocytes [8,9] Here, we applied the < /b> new assay, together with the < /b> flow cytometry, to quantify the < /b> number of < /b> recently produced B and T ... number of < /b> KRECs or TRECs (copies/PBMC) is calculated with the < /b> following formula: mean of < /b> KRECs or TRECs quantity mean of < /b> TRAC quantity / (1) The < /b> mean quantity of < /b> TRAC has to be divided by because ... patients compared to controls This result suggests that one of < /b> the < /b> reasons of < /b> the < /b> early IgM decrease could be attributed to the < /b> reduced production of < /b> new B lymphocytes because if Ig production...
... [18] The < /b> boxplot is based on robust statistics which are more resistant to the < /b> presence of < /b> outliers than the < /b> classical statistics based on the < /b> normal distribution [19] Hence, the < /b> data sets of < /b> the < /b> ... protection agents In this research using foliar broths to manufacture GNPs, the < /b> distribution of < /b> the < /b> total proteins (CP) versus the < /b> conversion of < /b> Au(III) is depicted by Fig Other < /b> than that of < /b> the < /b> ... anti-oxidant ability with the < /b> bioreduction of < /b> Au(III) in biosynthesis of < /b> GNPs In summary, being parameter targeted, the < /b> methodology demonstrated strengthens the < /b> pertinence with respect to biocompounds...
... ≤ k ≤ N , is the < /b> sheave of < /b> strongly contact k- forms r(c) Let k = d k 1 + k In [3] and [5] the < /b> authours proved the < /b> softness of < /b> r r(c) r(c) the < /b> sheaves k , considered the < /b> quotient sheaves k ... · + k We denote by pk : k → kthe < /b> morphism of < /b> sheaves defined by pk ρ = ρq , r,q r r+1 r,q for ≤ q ≤ kk = ker pk , ≤ k ≤ n, is the < /b> sheave of < /b> contact k- forms r,0 r(c) k = ker pk r ,k n , ... simplicity, r r we will solve this problem in the < /b> case of < /b> r = and r = Then the < /b> other < /b> cases follow by the < /b> same method Notations and Premilinaries Throughout this paper, the < /b> following notations will be...
... 3, the < /b> tree on the < /b> left side is of < /b> the < /b> first class while the < /b> other < /b> belongs to the < /b> second class For simplicity of < /b> the < /b> remaining presentment, we describe the < /b> core idea of < /b> the < /b> above involution in the < /b> ... his study The < /b> author is also very grateful to the < /b> referees for their valuable suggestions and comments This work was supported by the < /b> 973 Project, the < /b> PCSIRT Project of < /b> the < /b> Ministry of < /b> Education, ... first class consists of < /b> trees in which there is at least one colored leaf in the < /b> lowest two levels (the < /b> two levels furthest to the < /b> root) and to the < /b> left of < /b> which all vertices in the < /b> same level have...
... with the < /b> advent of < /b> the < /b> N activity, indicates a relationship ase between both enzyme activities The < /b> low NR activity (!1 nmol N0 DW!h-!) of < /b> -mg2 nodulated plants could be greatly increased by nitrate ... nitrate supplied via the < /b> roots This inducible NR activity was consistently ) DW-h- was highest in the < /b> younger expanded leaves that showed the < /b> highest nitrate content Studies are in progress to ... expanded, the < /b> NR activity decreased in the < /b> previous leaf and the < /b> highest enzyme activity was found again in the < /b> new leaf (Fig 2) When the < /b> nitrate supply was withdrawn, the < /b> enzyme activity recovered its...
... and the < /b> nonquantitative nature of < /b> the < /b> bulk PCR approach employed by the < /b> investigators Furthermore, for most of < /b> the < /b> study total B cells were studied without differentiating specific B cell subsets ... generated B cells The < /b> growing availability of < /b> patients treated with B cell depletion should allow investigators to understand the < /b> determinants that underlie B cell reconstitution in different autoimmune ... CD27, it is tempting to postulate that upon profound B cell depletion there could be a re-enactment of < /b> early B cell ontogeny and that the < /b> cells described in the < /b> report by Rouzière and coworkers...