... L2,3-XES of Fe2O3, FeSe1-yTey and Fe metal (b) Ratio of integrated intensities (I2/I3) of XES L2 and L3 lines X-Ray Spectroscopy Studies of Iron Chalcogenides 33 The similarity between XAS and XES of ... λ–(BETS)2FeCl4 is one of the most attractive materials in the last two decades for the observation of interplay of superconductivity and magnetism and for the synthesis of magnetic conductors and superconductors ... correlations of FeSe1-yTey As discussed above, due to the unstable phase of stoichiometry FeSe and efforts to more thoroughly understand the origin of superconductivity in this class of materials, of worthwhile...
... residues via networks of water-mediated hydrogen bonds The backbone nitrogen and carbonyl oxygen of Thr29 and the side chain oxygen of Ser54 are hydrogen-bonded to the O4 atom of pantoate via a network ... A-chain and B-chain of nPS, and in the case of His37, the v1 torsion angle changes from 63.34° to )67.80° and )52.11° for the A-chain and B-chain of nPS, respectively The imidazole ring of His34 ... strongly suggests that binding of pantoate and ATP preserves the dimer symmetry in solution In an effort to unambiguously identify and understand the nature of binding of pantoate at the ATP-binding...
... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of ... observation and dynamics of specific amide protons in T4 lysozyme Biochemistry 24, 817–822 McIntosh LP & Dahlquist FW (1990) Biosynthetic incorporation of 15N and 13C for assignment and interpretation of ... resonance assignments of the [15N]-HSQC spectrum, where it is often difficult to assess the magnitude of chemical shift changes upon ligand binding in [15N]-HSQC spectra of uniformly labelled...
... region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(b1– 3)GalNAc(b1-]n, which may be O-sulfated on the C4 and ⁄ or C6 of GalNAc and C2 of ... availability of comprehensive parameters describing the structures of a wide range of oligosaccharide structures The complete assignments of 1H and 13C NMR spectra from a series of disaccharides and tetrasaccharides ... allowing an understanding of species, tissue, age and pathology related differences and the estimation of changes in CS ⁄ DS abundance and composition However, the reduction of the polymer to its...
... shifts of avins in solutionand FAD bound to TrxR in the oxidized (A) and reduced state (B) Reduced enzymes As the fundamental concept for the interpretation of the 13C and 15N chemical shifts of ... TARFH2 and FMNH2, are C(5a), C(7), C(9a) and C(10a), and as already mentioned above, C(2) (Fig 7B) With respect to the 13C chemical shifts of FMNH2, C(2), C(5a), C(9a) and C(10a) of wild-type and ... the chemical shifts of C(4a) and C(5a) and C(7) parallel that of N(10), i.e follow the degree of increasing sp2 hybridization of N(10) in the order C138S, C138S + PMA, E159Y and native protein...
... process and show a suitable signal dispersion, which allows high-resolution characterization Therefore, the combination of these two sets of data (1H–15N and 1H–13C) provides a detailed characterization ... Another N-terminal fragment of Ab, Ab(1–16), forms stable oligomers in the presence of both copper and zinc [26] On the other hand, binding of low concentrations of copper or zinc ions to the ... Tyr10 is not involved in binding of the metal Instead, the e and d resonances of the histidines show significant intensity losses, of > 80% The d-proton signals of His6 and His13 show some induced...
... (UDP-glc), b and c phosphate of adenine nucleotide phosphates (ATPb, and ADPb plus ATPc, respectively) The spectrum consisted of 1280 scans and was acquired at 161 MHz with a spectral width of 16 kHz, ... phase To understand further the regulatory mechanism of nucleotide production, dependency of the cellular energetic on the carbon source was investigated by measuring cellular ATP and ADP by the ... ADP and Fig Representative 31P NMR spectra of metabolites from the XMP overproducer in phase I (A) and II (B) In spectra A and B, cell density during NMR observations were approximately gÆL)1 and...
... ligand solution with the proteins Aliquots of a protein stock solution were added to a ligand solution The fluorescence of the ligand was measured after each addition of the protein stock solution ... The Kd values for NP and MP were determined by titrating a protein solution with the ligands Aliquots of a stock solutionof one of the ligands was added to the protein solution Fluorescence ... 35, and 70 after the addition of the enzyme The top spectrum is that of DHMP for comparison Only the NMR signals of the 2¢ and 3¢ protons of DHNP and DHMP are shown The chemical structures of...
... chain of Arg78 The side chain of Arg78 is less well defined, as in the case of aqueous solution, because of the absence of interactions with the side chains of Asp81 (in the agonist analogue) and ... binding and affinity for I-Au because of occupation of the P1 pocket Recent thermodynamic and kinetic studies of the binding of TCRs to peptide–MHC ligands suggested that the low affinity of the ... (1999) Designand synthesis of a potent cyclic analogue of the myelin basic protein epitope MBP72-85: importance of the Ala81 carboxyl group andof a cyclic conformation for induction of experimental...
... symmetry of the three subdomains designated a, b and c, and that the protein has two sugar-binding sites in subdomains a and c [24,25] Therefore, determination of the sugar-binding ability and specificity ... amide and amide proton of Asp18, and side chain amide and amide proton (marked by ‘sc’) of Trp33 in EW29Ch during the titration of lactose (A, top), b-Me-Gal (A, bottom), melibiose (B, top) and ... spectra for the mixture of lactose (5 mM) and EW29Ch (50 lM) (A) Reference TOCSY spectrum of the mixture of lactose and EW29Ch at a ratio of 100 : (B) The STD–TOCSY spectrum of the same sample was...
... 3.10 Plots of the number of assigned NOEs of (a) SAM60 and (b) SAM76 as a function of the range of NOEs and the residue numbers (lower panel), respectively 82 Figure 3.11 Sequential and medium-range ... 1.4 Scope of research and outline of the thesis CHAPTER MATERIALS AND METHODS 2.1 Media 2.2 Expression vector construction 2.3 Expression and purification of DLC1-SAM 2.3.1 Expression of DLC1-SAM ... DISCUSSION 3.1 Expression and purification of DLC1-SAM 3.1.1 Expression and purification of SAM60 3.1.2 Expression and purification of SAM76 3.2 NMR resonance assignment of DLC1-SAM 3.2.1 Backbone...
... the study and takes responsibility for the integrity of the data and the accuracy of the data analysis MK contributed to study concept and design, to analysis and interpretation of data and to drafting ... concept and design, to analysis and interpretation of data and to drafting of the manuscript and critical revision for important intellectual content NK contributed to study concept and design, ... manuscript and critical revision for important intellectual content SM contributed to study concept and design, to analysis and interpretation of data and to drafting of the manuscript and critical...
... relationship of age, gender, handedness, and sidedness to the size of the corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness on ... levels of active latency were associated with concentration ability, and passive latency with verbal proficiency and recall [72] The decreases in P3 and novelty P3 with increasing age, and indeed ... descriptive and limited in power and generalizability Still lacking are large-scale, blinded, randomized, prospective studies of ERP-guided therapy; however with decreasing costs of signal acquisition and...
... context, and then present and discuss eight case studies found in the literature 1.1 Software development; problemsand remedies To develop and maintain software is often referred to as software ... 1.1.1 Problems with software development Software development can often be challenging There are many examples of software projects that have failed The much-cited Standish report on software ... Disasters [7] and Software Runaways [8] After listing all these problems that exist in software, you may ask: are all software systems that bad? That is not so, there are a lot of software projects...
... the side of the cords These enlarge and elongate and make their way toward the surface of the ground They are at first very minute and grow from the size of a pinhead to that of a pea, and larger ... Toxicology of Mushrooms, and Characters of Mushrooms, to which their names are appended, and also to Dr Chas Peck, of Albany, N Y., and Dr G Bresadola, of Austria-Hungary, to whom some of the specimens ... part of the margin of the pileus during the young stage rests between the lower and upper part of the annulus, i e., the thick veil is attached both to the inner and outer surface of the margin of...
... activity [10] The structural characterizationof numerous complexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17] The structure of human HPRT can be divided ... domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... the base of bound GMP is stacked between Val143 and Phe194 N1, N2 and O6 of the nucleotide form hydrogen bonds to main chain atoms, whereas the side chain of Lys173 interacts with both O6 and N7...
... reduction of ferredoxin Thus, the identification andcharacterizationof enzymes that transfer electrons to ferredoxins in vivo is of particular importance for the improvement of this coupled assay and ... acetyl-CoA and enzyme solutions to the mixture, and the decrease in A340 as a result of NADH oxidation was measured One unit of enzyme activity was defined as the reduction of lmolÆmin)1 of NADH ... between CO2 and the carboxyl group of 2-oxoglutarate [44], and therefore interferes with the detection of [14C] pyruvate Instead, the rate of pyruvate formation was determined by monitoring the...
... heating and power generation is considered the main sourceof SO2 and particulate contamination, which damage human health.52) Domestic sources of SO2 are associated with the use of coal and other ... conducted to confirm and extend the U.S.A case definition of MCS The results showed a significant overlap of MCS, chronic fatigue syndrome (CFS) and fibromyalgie, and that no standard diagnostic procedure ... consideration of public health.38) The relationship between moisture and temperature on skin and upper airway symptoms was investigated and showed that skin dryness and rashes, pharyngeal dryness, and...
... mutation of deoxyribonucleoside kinase L Egeblad-Welin et al Mutants of substrate-binding residues: Y70 and Q81 Y70W showed an increase in Km values for both dThd and dCyd, of approximately 200- and ... value for dThd and dCyd, of approximately 200- and 100-fold, respectively The kcat values were only decreased approximately twofold for both substrates Phosphorylation of nucleosides and nucleoside ... phosphorylation of dGuo was reduced markedly Structure of Dm-dNK-E52D in complex with dTTP The structure of one of the mutants, E52D, was solved ˚ using X-ray crystallography at a resolution of 2.5 A...
... properties To find the optimal buffer conditions for the biochemical characterizationof the enzymes, we carried out an analysis of the NaCl requirements and pH optimum of VsEndA and VcEndA The optimum ... sequences of VsEndA (Fig 1) The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA Expression and purification From L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and ... the salt optima of the enzymes are fine-tuned to match the salinity of their environment The outer membrane and cell wall of Gram-negative bacteria not restrict passage of ions, and the periplasmic...