nod scid mice bone marrow on the frequency of human hematopoietic stem cells
báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... comparison, the expression level of non-target genes of the same number was also correlated with the miRNA expression Density plot of correlation coefficient distribution was generated in R environment ... cells only, node C contained the genes upregulated in EB only, and node D contained the genes that were upregulated in adult cells HMVEC = human microvascular endothelial cells, HUVEC = human umbilical ... cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the expression...
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Báo cáo y học: "Influence of sequence identity and unique breakpoints on the frequency of intersubtype HIV-1 recombination" pot
... Increased frequency of recombination in vitro can be misleading considering the inability to reconstitute the conditions of endogenous HIV-1 reverse transcription Compositions of buffers, concentrations ... oligonucleotide specifically onto the donor template Reverse transcription was started by the addition of HIV-1 RT at a final concentration of 400 nM and carried out for 60 Synthesis of the second ... in the presence or absence of HIV-1 nucleocapsid protein (NC) (schematic, Figure 2D) The addition of 5' non-homologous nt's to the 5' end of the donor prevents transfer from the end of the donor...
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Báo cáo khoa học: Effect of heliquinomycin on the activity of human minichromosome maintenance 4/6/7 helicase pdf
... Effect of heliquinomycin on the RNA priming activity of the DNA polymerase-a/primase complex (A) Top: effect of increasing concentrations of heliquinomycin (HQ) on the RNA priming action of the ... primer of approximately 10 nucleotides was synthesized only in the presence of the above complex The synthesis of the RNA primer was inhibited in the presence of heliquinomycin at an IC50 value of ... for the T antigen and an average of the values was plotted (B) Top: effects of increasing concentrations of heliquinomycin on the DNA helicase activity of the Werner protein Bottom: proportion of...
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the johns hopkins university press the medicalization of society on the transformation of human conditions into treatable disorders apr 2007
... The Medicalization of Society This page intentionally left blank The Medicalization of Society On the Transformation of Human Conditions into Treatable Disorders peter conrad The Johns ... www.press.jhu.edu Library of Congress Cataloging-in-Publication Data Conrad, Peter, 1945– The medicalization of society : on the transformation of human conditions into treatable disorders / Peter Conrad p ; ... in investigating the rise of medicalization The focus was on the creation (or construction) of new medical categories with the subsequent expansion of medical jurisdiction Concepts such as moral...
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Effect of Na2SO4 additive in positive electrodes on the performance of sealed lead-acid cells for electric scooter applications ppt
... the performance of VRLA cells Based on the results presented herein, we can conclude the following Ž1 The XRD analyses showed that the major constituent of the additive Na SO4 in the cured plates ... work, we studied the effect of Na SO4 additive in the positive electrode on the performance of VRLA cells for ES applications Experimental procedures 2.1 Cell construction Each cell contained two ... the paste was - wt.% The current density was controlled at mA cmy2 and the formation capacity had a theoretical capacity of about 200% under the formation After formation, the plates were washed...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... by the proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff ... and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the aggregates ... development of the limbs, however, BMPs along with other regulators also mediate the replacement of chondrogenesis by endochondral ossification comprising chondrocyte maturation, hypertrophy, transition...
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Báo cáo y học: "Impact of bile acids on the growth of human cholangiocarcinoma via FXR" ppt
... expressed strongly in 60% and moderately in 40% of the normal tissues (Figure 4B) There was strong staining of FXR in the nucleus of the normal bile duct, which reflected the high expression of FXR ... expression (Figure 1C) of FXR On the contrary, treatment by the conjugated bile acids (GCA, GDCA and GCDCA) decreased the mRNA and protein expression of FXR According to density level, the mRNA ... Combined treatments of GS and CDCA could reverse the regulating effect of CDCA on the expression of FXR Additionally, when treating cells with conjugated bile acids-GDCA, FXR expression was decreased...
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Báo cáo y học: "Comparative study on the effect of human " pptx
... MFI of hBST-2 on the surface of whole cells, and that in the square in the plot indicates MFI of hBST-2 on the surface of p24-postive cells The amount of p24 in the culture supernatant (E), the ... percentage of p24-positive cells (F), the level of hBST-2 on the surface of whole cells (G), and the level of hBST-2 on the surface of p24-positive cells (H) following infection with either wild-type ... [7] Therefore, the relevance of surface hBST-2 down-regulation and the antagonistic action of Vpu on the tethering ability of hBST-2 remain unclear We first set out to analyze the level of endogenous...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated state has its own copy or copies of the genome with the only exception being cells ... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of ... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have...
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Báo cáo y học: "The potential of human regulatory T cells generated ex vivo as a treatment for lupus and other chronic inflammatory diseases" pot
... humans, 6–18% of CD4+ T cells constitutively express CD25 [11–17] Purified CD4+ CD25+ cells not proliferate in response to cross-linking of their TCRs They inhibit the activation of other T cells ... small number of CD25+ 243 Arthritis Research Vol No Horwitz et al cells in the naive fraction Although
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J ... all donors tested Another factor to consider is whether the age of the donors had an influence on the results of this study Four donors were over 70 years old and one donor was aged 19 The relative ... Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during...
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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
... activation of basal/ stem cells and their progression toward a metastatic status under the influence of Hh signalling remain to be further elucidated To further characterize the basal cells during ... and sham controls (Figure 3C; (a) indicated two small lymph nodes (on the left) from the sham controls and two enlarged lymph nodes (on the right) from the pCX-shh-IG-injected mice) The metastatic ... reconstituted in vivo and the effects of Hh overexpression on the normal prostate stem cells was shown by the transformation of P63+/Patch1(Low or -) and P63+/Smo (Low or -) quiescent basal cells...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... differentiation of rat bone marrow- derived mesenchymal stem cells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stem cells ... 2007;Extracellular matrix made by bone marrow cells facilitates expansion of marrow- derived mesenchymal progenitor cells and prevents their differentiation into osteoblasts J Bone Miner Res (12):1943-1956 ... mesenchymal stem cells from marrow Tissue Eng (4):415-428 129 Beresford JN, Graves SE, Smoothy CA 1993;Formation of mineralized nodules by bone derived cells in vitro: a model of bone formation? Am J...
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Effects of combined mechanical and pulsed electromagnetic field stimulations on the osteogenesis of bone marrow stem cells
... (cancellous) bone Cortical bone forms the dense outer shell of most bones and it accounts for 80% of the total bone mass Cortical bone is mostly found in the shaft portion of long bones and the outer ... cancellous bone On the other hand, cancellous bone consists of a network of struts surrounding interconnected spaces It is the spongy interior bone tissue which makes up 20% of the total bone mass ... cancellous bones are different in that compact bone consists of haversian sites and osteons while cancellous bone does not Secondly, bone surrounds blood in compact bone while blood surrounds bone in the...
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Tissue engineering approaches to tendon repair studies on the use of bone marrow stromal cells and knitted poly (d, l lactide co glycolide scaffold
... the calcaneus bone As organs, tendons consist of three parts: the muscle attachment region, the substance of the tendon itself, and tendon to bone insertion region These three parts vary in their ... and the inorganic components Collagen constitutes approximately 90% of the total protein of the tendons or 65-75% of the dry mass of tendons Elastin accounts for only about % of the dry mass of ... tendons of the hand Following a full transection, there is a spontaneous retraction of the cut tendon ends In the Achilles tendon of both a rat and rabbit model, the gap formed by the retraction...
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Báo cáo y học: "The Influence of Hyperbaric Oxygen Treatment on the Healing of Experimental Defects Filled with Different Bone Graft Substitutes"
... residual bone graft particles were surrounded by a combination of newly formed bone and cartilage tissue The new bone formation in the defects of the CPCBB group was more prominent when compared to the ... with newly formed bone in the control groups which had received four week of HBOT (H&E×200) (Nbf; New bone formation, Bm; Bone marrow) Table 1: Overall results of the bone histomorphometry and ... in host tissue – bone graft interactions The use of adjunctive HBOT to improve bone healing in autogenous bone transplants has been investigated in the histologic observation of the iliac grafts...
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báo cáo hóa học: " The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos on the expression of glial fibrillary acidic protein in the hippocampus of adult mice" pptx
... The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos on the expression of glial fibrillary acidic protein in the hippocampus of adult mice Journal of ... expression [17,18] The effect of dermal application of CPF on GFAP expression in the hippocampus has not been reported The aim of this study was to determine the expression of GFAP in the hippocampal ... Effect of concurrent application of heat, swim stress and repeated dermal application of chlorpyrifos on the hippocampal neurons in mice Folia Neuropathol 2009, 47(1):60-68 Gordon CJ, Leon LR: Thermal...
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Báo cáo hóa học: " Research Article Effects of Carrier Frequency Offset, Timing Offset, and Channel Spread Factor on the Performance of Hexagonal Multicarrier Modulation Systems" doc
... two-dimensional (2D) case Specifically, in OFDM system with symbol period T and subcarrier separation F, the transmission functions of OFDM system consist of translations and modulations of a single ... usually depends on the precision of carrier frequency offset estimation and symbol timing A generalized framework for the prediction of OFDM system performance in the presence of carrier frequency offset ... channel dispersion point of view Synchronization is considered as a key factor of designing multicarrier modulation system receiver The synchronization precision significantly affects the receiver...
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Báo cáo hóa học: " On the Compensation of Delay in the Discrete Frequency Domain" pot
... satisfy these properties, then such an expression is only approximate To facilitate the application of the general FIR filter analysis of Section 3, let the signal s(n) pass through the transmission ... Reconstruction SNR for filterbank FDAF equalisation of constant delay Figure 8: Reconstruction SNR for filterbank FDAF equalisation of the linear delay channel The solid trace of Figure shows the ... applications, this may be the most appropriate measure of quality In others, including conventional equalisers and That is, the number of bins in the frequency domain implementation is equal to the...
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Báo cáo khoa học: "Effects of Combination Dietary Conjugated Linoleic Acid with Vitamin A (Retinol) and Selenium on the Response of the Immunoglobulin Productivity in Mice" pdf
... IgG concentrations of CONT, CLA1, CLA2, Effects of Combination Dietary Conjugated Linoleic Acid with Vitamin A (Retinol) and Selenium on the Response of the Immunoglobulin Production in Mice ... tion o f th e gu t lu m e n la va ge After blood collection, the end of the duodenum and the cranial part of the cecum were tied and both ends were cut One end of the small intestine was at the ... immunoglobulin concentrations than those of all the 0.5% CLA groups Even serum IgG concentration in 1.5% CLA group was decreased compare to that of control Over-dosage of CLA may affect the depression of...
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