nmr structure of sp b peptides

Tài liệu Báo cáo khoa học: Solution NMR structure of an immunodominant epitope of myelin basic protein Conformational dependence on environment of an intrinsically unstructured protein doc

... family Biotechnol Focus 8, 18–22 Libich DS, Robertson VJ, Monette MM & Harauz G (2004) Backbone resonance assignments of the 18.5 kDa isoform of murine myelin basic protein (MBP) J Biomol NMR 29, ... dodecylphosphatidylcholine (DPC-d38) Direct application of solution NMR to membrane-associated MBP is problematic because of the reduced mobility of the protein in a reconstituted protein–lipid assembly ... 17 by site-directed spin labeling J Biol Chem 278, 29041–29047 Bates IR, Feix JB, Boggs JM & Harauz G (2004) An immunodominant epitope of myelin basic protein is an amphipathic alpha-helix J Biol...

Tài liệu Báo cáo khoa học: NMR structure of AII in solution compared with the X-ray structure of AII bound to the mAb Fab131 pptx

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... of the structure of AII in solution with the X-ray structure of AII complexed to the Fab131 mAb In Fig 7A, a sequence alignment is represented of the backbone of the conformational ensemble of ... number range in the X-ray structure of AII Fig (A) Sequence alignment of the fragment 4–7 of the backbone of the 13 ensemble solution structures of AII (brown colour) superimposed on the X-ray structure ... surface area of the free mAb; (b) it creates space from which it is easy to Ó FEBS 2003 Comparison of the free and bound structure of angiotensin II (Eur J Biochem 270) 855 Fig Structure of AII (A)...

Tài liệu Báo cáo khoa học: Solution NMR structure of ﬁve representative glycosylated polyene macrolide antibiotics with a sterol-dependent antifungal activity doc

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... Michels, B. , Raffard, G & Dufourc, E.J (1997) Association of polyene antibiotics with sterol-free lipid membranes II Hydrophobic binding Ó FEBS 2002 NMR structure of glycosylated polyene antibiotics ... analysis of rimocidin The small number of restraints that could be applied in the region of C4–C8 segment and for the two aliphatic side chains (Table 2; Fig 1) yielded a number of different possible ... to the biomembranes The five NMR solution structures described here, are important steps in the rationalization of their selective affinity for sterols in biomembranes, and in the design of new...

Báo cáo khoa học: Structure of amyloid b fragments in aqueous environments docx

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... conformations of other Ab fragments, including fulllength Ab The two most abundant forms of Ab are the 40 and 42 residue peptides, Ab1)40 and Ab1)42 Both forms are capable of assembling into b- sheet fibrils ... et al Structure of amyloid b in aqueous environments A C B Fig (A) Sequence of amyloid b peptide (Ab) The regions with underbars correspond to Ab10)24 and Ab28)42 (B) Schematic diagram of the ... efficiently, because of the absence of an Ab C-terminal region Conformation of Ab28)42 in aqueous environments Structural studies of amyloid fibrils from Ab have been performed using CD [30], X-ray fiber...

Báo cáo khoa học: NMR structure of the thromboxane A2 receptor ligand recognition pocket pot

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... the peptides adopt a turn conformation [45–49] But the deﬁned distance between both of the ends of the peptide were not conclusive because the structures of the N- and C-terminal residues of peptides ... (1976) Contribution of NMR spectroscopy to the study of structure- function relations of proteins and peptides Prog Nucl Magn Reson Spectros 10, 41–82 22 Pardi, A., Billeter, M., Braun, W & Thrich, ... Alderfer, J.L & Albert, A.D (1995) Structure of the carboxy-terminal domain of bovine rhodopsin Nat Struct Biol 2, 832–834 46 Yeagle, P.L., Alderfer, J.L & Albert, A.D (1995) Structure of the third...

Báo cáo khoa học: Multifunctional host defense peptides: functional and mechanistic insights from NMR structures of potent antimicrobial peptides docx

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... fragments of human b- defensin-3, as they preferred to be associated with the toroidal pores of bicelles Structures and mechanisms of antimicrobial peptides in a model outer membrane of bacteria ... conformations, but also that peptides may form b- strands and b- turn structures in LPS micelles [52,54,57] With a set of designed peptides, the LPS-bound structures reveal multiple b- turn and b- strand structures ... of amphibian antimicrobial peptides: linking structure to function? BBA Biomembr 1788, 1639–1655 Hallock KJ, Henzler Wildman KA, Lee DK & Ramamoorthy A (2002) Sublimable solids can be FEBS Journal...

Báo cáo khoa học: Quenched hydrogen ⁄deuterium exchange NMR characterization of amyloid-b peptide aggregates formed in the presence of Cu2+ or Zn2+ ppt

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... classic fibrillar morphology, with an average fibril diameter of about nm It is well known that Ab(1–40) and Ab(1–42) fibrils consist of bundles of thinner filaments, each having a cross-section of about ... ensemble of peptides of various lengths, each of which has distinguishing biophysical properties Fragments of 39–43 residues are all clinically relevant, but the most abundant are Ab(1–40) and Ab(1–42) ... analysis of metal-complexed Ab aggregates A D B E C A Olofsson et al F Fig Tapping mode AFM images of recombinant Ab(1–40) and Ab(1–42) aggregates (A–C) Morphology of Ab(1–40) in the presence of Cu2+,...

Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/triﬂuoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot

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... (A) between each structure and the best structure calculated using the backbone atoms (N, Ca, C¢, O) Residues Fig Representation of (1–96)Vpr structure Upper panel: Backbone superimposition of ... domain backbone of the intact protein ˚ (1–96)Vpr (blue) (rmsd of 2.6 A) The (54–78) domain backbone of the C-terminal (52–96)Vpr (green) is superimposed with the (54–78) region ˚ backbone of the ... orientation of the a-helices in the 3D structure of the protein The backbone is represented in blue and the hydrophobic amino acid side chain coloured pink Ó FEBS 2002 NMR Structure of the HIV-1...

Báo cáo khoa học: The crystal structure of a xyloglucan-speciﬁc endo-b-1,4glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate speciﬁcity potx

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... (Asp34 and Asp458) Thus, it is conceivable that XEG and OXG-RCBH recognize the backbone of the b- 1,4-glucan in a similar manner, despite their differences in substrate specificity and mode of hydrolysis ... map of XEG for the region corresponding to the exo-loop of OXG-RCBH The coordinates and structure factors have been deposited in the Protein Data Bank (PDB) (accession code 3A0F) The structure of ... substrate specificities of XEG and OXG-RCBH depend on a limited number of residues in the substrate binding cleft Experimental procedures Purification of XEG The gene encoding the mature region of...

Báo cáo khoa học: NMR solution structure of Cn12, a novel peptide from the Mexican scorpion Centruroides noxius with a typical b-toxin sequence but with a-like physiological activity doc

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... 3D structures are known were included The 3D structures of three pharmacologically different classes of Na-ScTxs are known: a Na-ScTxs (AaHII, BmKM1, BmKM2, BmKM4, BmKM8, BmK-aTx16, BmK-aIT, Bs-mktx, ... databanks (PDB entry 1PE4 and BRMB number 5913) Discussion NMR solution structure of Cn12 Figures 4, and summarize the most important data obtained from the NMR analysis of pure Cn12 It was possible to ... constants used for the structure calculation Figure 4B shows a ribbon diagram of Cn12, in which the most obvious 3D elements are shown This was possible because of the use of 850 distance constraints,...

Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

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... Content of the asymmetric unit Number of nonhydrogen atoms Number of protein molecules Number of water molecules Number of glycerol molecules Number of sodium ions Number of magnesium ions Number of ... facilitates substrate binding as well The hydrogen bond between the backbone nitrogen atom of Asp291 and the 3phosphate of the substrate is the only interaction with the protein backbone; all other ... is a single hydrogen bond between the substrate, phytate and the backbone of PhyK involving Asp291 In the structure of phytate bound to AppA, this hydrogen bond is also observed and, moreover,...

Tài liệu Báo cáo khoa học: The crystal structure of human a-amino-b-carboxymuconatee-semialdehyde decarboxylase in complex with 1,3-dihydroxyacetonephosphate suggests a regulatory link between NAD synthesis and glycolysis ppt

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... available structural data suggest Fig Ribbon representation of the overall structure of hACMSD The (a ⁄ b) 8 barrel domain is colored in light blue and the ACMSDspeciﬁc small insertion domain in blue ... catalysis, not only because it contributes to Zn2+ coordination but also because of its direct involvement in substrate binding (Fig 3A ,B) Indeed, as detailed above, Asp291 stabilizes DHAP through ... 2.6 94 Number of protein atoms Number of solvent molecules Number of DHAP ⁄ Zn atoms Number of glycerol atoms R-factor (%) R-free (%) ˚ rmsd bond lengths (A) rmsd bond angles (°) ˚ Mean B- factor...

Tài liệu Báo cáo khoa học: Structure of the putative 32 kDa myrosinase-binding protein from Arabidopsis (At3g16450.1) determined by SAIL-NMR docx

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... GalNAca1-3(Fuca1-2)Galb1-3(Fuca1-4)GlcNAcb1-3Galb14Glc-PA and Neu5Aca2-6Galb1-4GlcNAcb1-2Mana16(Neu5Aca2-3Galb1-3(Neu5Aca2-6)GlcNAcb1-4(Neu5Aca26Galb1-4GlcNAcb1-2)Mana1-3)Manb1-4GlcNAcb1-4GlcNAc-PA were obtained ... (code no 210.1) Galb1-4GlcNAcb1-2Mana1-6 (Galb1-4GlcNAcb1-2 Mana1-3)Manb1-4GlcNAcb1-4(Fuca1-6) GlcNAc (code no 210.4) GlcNAcb1-2Mana1-6(GlcNAcb1-2Mana1-3) Manb1-4(Xylb1-2)GlcNAcb1-4 (Fuca1-3)GlcNAc ... sugar (black) (B) FAC binding assay for Gala1-4Galb1-4Glc-PA (red) and control PA sugar (black) (C) Overlay of the 1H-15N HSQC spectra of uniformly 15 N-labeled At3g16450.1 in the absence (black)...

Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx

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... compilation ª 2007 FEBS 4229 NMR structure and dynamics of eRF1 middle domain E V Ivanova et al A B Fig Ribbon representation of the backbone of the M domain of human eRF1 The variable radius of the cylinder ... comparison of part of the protein backbone structure of the representative solution structure of the human eRF1 M domain and the Ca trace in the crystal structure of RF1 in the whole ribosome structure ... Gronenborn AM (1990) Analysis of the backbone dynamics of interleukin-1 beta using two-dimensional inverse detected NMR structure and dynamics of eRF1 middle domain heteronuclear 15N)1H NMR spectroscopy...

Báo cáo khoa học: Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 pdf

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... a13 contains strands b1 7, b1 8, b1 9 and b2 4, whereas the solvent-exposed second b- sheet harbors strands b2 0, b2 1, b2 2 and b2 3 The b- sheet at the interface to domain A has lower B factors (Fig 3) ... opposite barrel end The lengths of the b- strands in the barrel vary from two (b1 4) to seven (b1 1) residues As in other enzymes of family no 13, the regularity of the CDase TIM barrel is broken by the ... about 150 additional C-terminal residues that probably mediate starch binding [37–39] 3D structure The crystals of Se-labeled CDase belong to space group ˚ R32 with unit cell dimensions a ¼ b...

Báo cáo Y học: Solution structure of the Alzheimer amyloid b-peptide (1–42) in an apolar microenvironment Similarity with a virus fusion domain potx

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... DISCUSSION Fig Bundle of the best 10 structures of Ab-(1–42) after AMBER minimization, superimposed for: (A) backbone atoms of residues 8–38 ˚ (RMSD = 0.86 A); (B) backbone atoms of residues 8–25 ... 0.38 A); (C) backbone atoms of residues 26–27 (RMSD = ˚ ˚ 0.048 A); (D) backbone atoms of residues 28–38 (RMSD = 0.59 A) appears to be a genuine feature of the global structure of Ab-(1–42) At ... interaction and destabilization is different in the case of Ab, Fig Comparison of the shapes of the lowest energy structure of HA_fd (A) and of the 1–35 region of Ab-(1–42) (B) Residue side chains...

Báo cáo khoa học: Crystal structure of basic 7S globulin, a xyloglucanspeciﬁc endo-b-1,4-glucanase inhibitor protein-like protein from soybean lacking inhibitory activity against endo-b-glucanase doc

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... Yoshizawa et al Structure of Bg7S, a XEGIP-like protein of soybean Structured digital abstract l Bg7S binds to Bg7S by x-ray crystallography (View interaction) l Bg7S binds to Bg7S by cosedimentation ... compilation ª 2011 FEBS 1945 Structure of Bg7S, a XEGIP-like protein of soybean T Yoshizawa et al Fig Structure of Bg7S from soybean (A) Top and side views of the Bg7S tetramer A, B, C and D molecules ... Structure of Bg7S, a XEGIP-like protein of soybean Fig Structural basis for the lack of inhibitory activity of Bg7S against GH12 and GH11 enzymes (A) Structure of Bg7S superimposed on that of the...

Báo cáo Y học: Puriﬁcation, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx

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... degradation are uncertain A proteolytic splitting of the a-subunit by proteases may be possible, although the pH of 2.2 of the formic acid probably inhibits the activity of most peptidases Another postulation ... phosphate, pH 7.5; (b) Tris phosphate, pH 8.0; (c) potassium phosphate, pH 7.5; (d) Tris phosphate, pH 8.0 Crystals of (b) have been tested by X-ray analysis They diﬀracted up ˚ to 2.8 A but structure ... analyses of photoactive a-PEC peptides from phycobilisomes of M laminosus Preliminary results of crystallization and NMR spectroscopy offer reliable information on the relations between the protein backbone...

Báo cáo khoa học: X-ray structure of glucose/galactose receptor from Salmonella typhimurium in complex with the physiological ligand, (2R)-glyceryl-b-D-galactopyranoside pdf

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... (331); ZP_01169389.1, probable galactoside ABC transporter from Bacillus sp NRRL B- 14911 (353); ZP_00134897.2, periplasmic component of ABC-type sugar transport system, Actinobacillus pleuropneumoniae ... contribute to different degrees The Table Comparison of conformational changes Structures of GBP (GBP–GGal versus PDB entry 2FW0), ribose-binding protein (2DRI versus 1URP) and allose-binding ... GGal C Ca+2 GGal binding B Fig Structure of the GBP–GGal complex (A) Overall structure of GBP, color-coded using a scheme going from blue at the N-terminus, through the rainbow to red at the...

Báo cáo khoa học: Crystal structure of a cold-adapted class C b-lactamase potx

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... compilation ª 2008 FEBS C Michaux et al be partly responsible for the larger flexibility of the cold b- lactamase and for its ability to hydrolyze large substrates Both psychrophilic b- lactamases have ... This flexibility, in spite of the steric hindrance of the substrate, is thought to be partially responsible for its unexpected activity on large penicillin substrates The kinetic parameters of the ... characterized by a decreased number of hydrogen bonds, possibly rendering the structure more flexible To confirm this tendency, higher-resolution structures would be necessary to improve the accuracy of those...

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