nmr of membrane proteins in lipid environments the bcl 2 family of apoptosis regulators
Báo cáo khoa học: The proapoptotic member of the Bcl-2 family Bcl-2 / E1B-19K-interacting protein 3 is a mediator of caspase-independent neuronal death in excitotoxicity pot
Ngày tải lên :
22/03/2014, 17:20
... members of the Bcl- 2 family, including Bad, Bax, Bid, and Bim, permeabilize mitochondrial membranes [13] Bcl- 2 ⁄ E1B-19K-interacting protein (BNIP3) is a member of a unique subfamily of death-inducing ... proapoptotic member of the Bcl- 2 family However, unlike in other members of the Bcl- 2 family, the BH3 domain of BNIP3 is not required for its death-inducing activity Our results showing that full-length, ... (1999) Bcl- 2 ⁄ E1B 19 kDa-interacting protein 3-like protein (Bnip3L) interacts with bcl- 2 ⁄ Bcl- xL and induces apoptosis by altering mitochondrial membrane permeability Oncogene 18, 4 523 –4 529 Yang...
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Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
Ngày tải lên :
19/02/2014, 07:20
... beta-barrel membrane proteins J Biol Chem 27 6, 323 99– 324 02 Integral proteins in the mitochondrial outer membrane Tokuda H & Matsuyama S (20 04) Sorting of lipoproteins to the outer membrane in E coli Biochim ... identified no other inner membrane proteins in our vesicle preparation, we suggest that Gut2 is an outer membrane protein with its N-terminal FAD-binding domain facing the intermembrane space ... a-helical transmembrane segments, in support of the behavior of the protein in Triton X-114 The transmembrane segments A Integral proteins in the mitochondrial outer membrane corresponding to residues...
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The roles of shca proteins in response to oxidative stress 2
Ngày tải lên :
14/09/2015, 14:18
... described in Fig 4.5A -1 02- A p-Erk1 /2 Erk1 /2 H2O2(mM) 0.1 0 .2 0.4 0.5 pMSCVp52ShcWT 0.1 0 .2 0.4 0.5 pMSCVp52ShcS29A B p 52 p46 pMSCVp52S29A pMSCVp52WT ShcA-/- ShcA Fig 4.6 Ser29Ala mutation diminishes ... (B) Expression of p52ShcA or p52ShcA S29A in retroviral infected MEFs -104- A P-Erk1 /2 Erk1 /2 H2O2 - + Inhibitor (μM) control - + rott 2. 5 - + rott - + stau 0.5 - + stau B p42Erk2 p44Erk1 co nt ... anti-HA (p 52) IB: PKC δ H2O2 + - + p52Shc - + + p 52+ pkc - p 52+ pkc + IB: anti-HA (p 52) 2. 5 Fold 1.5 0.5 Fig 4.2C Association between p52ShcA and p46ShcA with PKC δ The experiment was done as Fig 2A,...
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Báo cáo sinh học: "Electroporation increases antitumoral efficacy of the bcl-2 antisense G3139 and chemotherapy in a human melanoma xenograft" pot
Ngày tải lên :
18/06/2014, 22:20
... and to increase the response of melanoma to therapy The rationale for the use of G3139 is based on the relevant role of bcl- 2 in melanoma cell survival and on the increased sensitivity of this ... Hollenstein U, Wolff K, Pehamberger H: Chemosensitisation 22 23 24 25 26 27 28 29 of malignant melanoma by BCL2 antisense therapy Lancet 20 00, 356:1 728 -1733 Bedikian AY, Millward M, Pehamberger H, Conry ... Cells in the exponential phase of in vitro growth were injected into the hind leg muscles of mice at × 106 cells/mouse in 0 .2 ml 0.9% NaCl solution CD-1 male nude, nu/nu mice, 6-8 weeks old and 22 -24 ...
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báo cáo hóa học:" Electroporation increases antitumoral efficacy of the bcl-2 antisense G3139 and chemotherapy in a human melanoma xenograft" ppt
Ngày tải lên :
20/06/2014, 04:20
... and to increase the response of melanoma to therapy The rationale for the use of G3139 is based on the relevant role of bcl- 2 in melanoma cell survival and on the increased sensitivity of this ... Hollenstein U, Wolff K, Pehamberger H: Chemosensitisation 22 23 24 25 26 27 28 29 of malignant melanoma by BCL2 antisense therapy Lancet 20 00, 356:1 728 -1733 Bedikian AY, Millward M, Pehamberger H, Conry ... Cells in the exponential phase of in vitro growth were injected into the hind leg muscles of mice at × 106 cells/mouse in 0 .2 ml 0.9% NaCl solution CD-1 male nude, nu/nu mice, 6-8 weeks old and 22 -24 ...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
Ngày tải lên :
18/06/2014, 19:20
... curcumin-induced apoptosis Disruption of the function of Bcl- 2 protein leads to permeabilization of the mitochondrial membrane [39] We therefore investigated the effects of curcumin on MMP using ... Accordingly, siRNA against Bcl- 2 increased the susceptibility of these CD34+ cell lines to DNR-induced apoptosis, indicating that Bcl- 2 down-regulation played an important role in this curcumin-induced ... Curcumin( M) 40 60 93 .25 6.47 97.31 80 37.65 19.14 23 .86 62. 33 80. 82 92. 88 72. 29 49.91 38 .27 KG1a Red 76.13 Kasumi-1 27 .68 50.05 84.76 20 .25 7 .25 0.36 15 .22 79.73 92. 74 99.63 7.08 U937 2. 58 Red/green...
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Báo cáo y học: "Coaggregation of Fc RI with Fc RIIB Inhibits Degranulation but Not Induction of Bcl-2 Family Members A1 and Bim in Mast Cells" ppsx
Ngày tải lên :
08/08/2014, 21:20
... Not Affect the Induction of A1 Apoptosis is regulated by members of the Bcl- 2 family A1, one of the antiapoptotic Bcl- 2 family members, is described as being important for the survival of mast cells ... apoptosis in mast cells.19 Antiapoptotic members of the Bcl- 2 family are needed for cell survival One of the murine prosurvival Bcl- 2 family members is A1, which plays a prominent role in preventing apoptosis ... Fc⑀RI-Induced Expression of Bim Is Not Inhibited by Coaggregation with Fc␥RIIB A key regulator of apoptosis is the Bcl- 2 family of proteins, which consists of proapoptotic and antiapoptotic proteins...
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Báo cáo khoa học: " AT-101, a small molecule inhibitor of anti-apoptotic Bcl-2 family members, activates the SAPK/JNK pathway and enhances radiation-induced apoptosis" pdf
Ngày tải lên :
09/08/2014, 10:20
... role in the cytotoxic effects of AT-101 Proteins of the Bcl- 2 family mediate mitochondrial permeability and are therefore the key regulators of the intrinsic apoptotic pathways [36] Bcl- 2 proteins ... M: BCL- 2 family proteins: regulators of cell death involved in the pathogenesis of cancer and resistance to therapy J Cell Biochem 1996, 60 :23 - 32 Simonian PL, Grillot DA, Nunez G: Bcl- 2 and Bcl- XL ... Thus, the anti-apoptotic function of Bcl- XL /Bcl- 2 is largely attributed to their ability to interact with pro-apoptotic members of the Bcl- 2 family through the hydrophobic BH3 binding α helix, thereby...
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Báo cáo y học: "The AP-2 family of transcription factors." potx
Ngày tải lên :
14/08/2014, 16:20
... Interacting proteins either modulate the activity of AP -2 proteins or are influenced in their function by binding to AP -2 proteins The tissue distribution and developmental functions of AP -2 transcription ... AP -2 might act as a negative regulator, inhibiting or modulating the transactivation capability or DNA-binding affinity of the other AP -2 family members The crystal structure of the AP -2 proteins ... XP _22 523 8 XP _21 7356 NP_958 823 XP _23 6975 XP _23 3 526 G gallus NP_990 425 NP_99 022 6 - XP_ 426 224 - X tropicalis AAD5 328 9 - - - - X laevis AAA499 72 - - - - D rerio NP_789 829 NP_001019836 - - Other AP-2...
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Báo cáo khoa học: Pituitary adenylate cyclase-activating polypeptide attenuates streptozotocin-induced apoptotic death of RIN-m5F cells through regulation of Bcl-2 family protein mRNA expression pdf
Ngày tải lên :
16/03/2014, 04:20
... accelerated apoptosis [2] The biochemical features of apoptosis include the activation of one or more cysteine proteases of the caspase family [23 ] To examine the possible involvement of caspase-3 in the ... down-regulation of Bcl- XL mRNA, they promoted expression of Bcl- 2 mRNA significantly According to these novel findings, the regulation of Bcl- 2, Noxa and Bax expression would be involved in the possible ... Pattou F (20 02) 1 ,25 -dihydroxyvitamin D3 protects RINm5F and human islet cells against cytokine-induced apoptosis: implication of the antiapoptotic protein A20 Endocrinology 143, 4809–4819 22 Konarkowska...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... (p0), the peptide (0.1 2 lm) was injected into the subphase, and the increment in surface pressure of the LPS film upon intercalation of the peptide dissolved in the subphase was monitored for the ... spot 6707 the peptide-induced membrane breakages, and substantial roughening of their surface The peptide might bind to the membrane surface in a carpet-like arrangement, inserting into the polar ... affect the integrity of the microbial plasma membrane and the synthesis of the microbial cell wall To the best of our knowledge, this study represents the first example of the effects of an antimicrobial...
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Báo cáo Y học: Unique structural determinants in the signal peptides of ‘spontaneously’ inserting thylakoid membrane proteins pptx
Ngày tải lên :
18/03/2014, 01:20
... mechanism involve binding of the intermediate-size protein to the membrane, after which both hydrophobic domains (one in the signal peptide, the other in the mature protein) insert into the membrane ... methionine at the end of A2 was substituted with leucine in both wildtype pPsbY (ÔPsbY-A2-metÕ) and the PsbY-A2/VV mutant (mutant PsbY-A2/VV-met) The proteins were synthesized in the presence of either ... pathways used for the targeting of thylakoid proteins in chloroplasts Traffic 2, 24 5 25 1 Lamppa, G.K (1988) The chlorophyll a/b-binding protein inserts into the thylakoids independent of its cognate...
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Báo cáo y học: "Summary The claudin multigene family encodes tetraspan membrane proteins that are crucial structural and functional components of tight junctions, which have important roles in regulating para cellular" pdf
Ngày tải lên :
14/08/2014, 21:20
... Claudin-4 Claudin-18a Claudin-3 Claudin-18b Claudin -20 Claudin- 12 Claudin-14 Claudin -2 Claudin-19b Claudin-19a Claudin-16 Claudin-7 Claudin-1 Claudin -21 Claudin -24 Claudin -22 Claudin -23 Figure A ... 564 92 CLDN19 149461 7471 92 298487 24 2653 49861 4 722 15 680178 621 628 CLDN21 6446 72 74 028 7 + 1000 427 85 538 42 743556 306454 75677 CLDN20 CLDN 22 CLDN23 137075 4 726 93 29 0789 71908 CLDN24 1001 324 63 ... 22 6 b: 22 8 24 ,25 1 24 ,488 9 .24 8. 32 CLDN11 3q26 One form 20 7 21 ,993 8 .22 CLDN 12 7q21 One form 24 4 27 ,110 8.8 CLDN14 21 q 22 Two variants: alternative splicing, coding unaffected 23 9 25 ,699 8.94 CLDN15...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
Ngày tải lên :
18/02/2014, 16:20
... CYPs in yeast cells 20 21 22 23 24 25 26 27 28 29 30 31 32 membrane into the matrix J Biol Chem 27 3, 20 941– 20 950 Sepuri NB, Schulke N & Pain D (1998) GTP hydrolysis is essential for protein import ... of PKA in the mitochondrial targeting of CYP2E1 The role of PKA in mitochondrial targeting of CYP2E1 was investigated using two approaches The first approach involved measuring the level of mitochondrial ... 1A1 proteins were localized inside of the inner membrane Moreover, the imported + ⁄ 1A1 and + 33 ⁄ 1A1 proteins were extractable with alkaline Na2CO3 as shown in Fig 2C, suggesting that both proteins...
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Tài liệu Báo cáo khoa học: Bone morphogenetic proteins in the early development of zebrafish pptx
Ngày tải lên :
19/02/2014, 00:20
... Follistatin, Chordin and Noggin [ 12] These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibiting these signaling molecules from binding to their receptors in ... (1996) dino and FEBS Journal 27 4 (20 07) 29 60 29 67 ª 20 07 The Author Journal compilation ª 20 07 FEBS M Kondo 24 25 26 27 28 29 30 31 32 33 34 mercedes, two genes regulating dorsal development in the ... suggested [28 ,29 ,35] The debate regarding the function of tsg in the BMP pathway probably will remain unresolved for some time Other factors affecting the BMP pathway Factors inhibiting BMP2b, other...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
Ngày tải lên :
21/02/2014, 03:20
... E192G M196V Y259Q D266N Y269A Q272R D266N/Y269A A271T/Q272R Y269A/A271T/Q272R D266N/Y269A/A271T/Q272R Wild-type Sendai N263D N263D/A266Y 0 .29 0 0 .23 7 0 .28 2 0.310 0 .26 2 0 .27 7 0.300 0 .26 5 0 .26 5 ... I108L D117H D137N A150T S170N V177T E192G M196V Y259Q D266N Y269A Q272R D266N/Y269A A271T/Q272R Y269A/A271T/ Q272R D266N/Y269A/ A271T/Q272R s-N263D s-N263D/A266Y 5¢-TTAACCTTCAtatgTGAGGGTATTTTTTG-3¢ ... on the opposite side of the N-terminal region on the molecular surface In the wildtype enzyme, the side chain of Asp266 interacted with the amino group of the main chain at Glu268 The oxygen of...
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Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx
Ngày tải lên :
07/03/2014, 01:20
... ABLIM -2 and -3, as STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates actin-binding in several proteins, such as villin ... protein Filamin NLS? Cross-linking proteins Filamin A NLS? Cross-linking proteins Transgelin ()) Cross-linking proteins Functions as scaffolds for signaling intermediates that stimulate actin elongation; ... actin is associated with rDNA genes, and microinjection of anti-actin Ig into the nuclei of HeLa cells inhibits pre-rRNA synthesis in vivo [25 ,34] The interaction of NM1 with actin in the initiation...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
Ngày tải lên :
07/03/2014, 16:20
... (20 02) Cathepsin L is essential for embryogenesis 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 and development of Caenorhabditis elegans J.Biol Chem 27 7, 3477–3486 Kirschke, H (1998) Cathepsin ... Domain Database of NCBI was screened with the protein coding sequence of clone of CLAP and the Fas I domains in the protein compared with 10 other fasciclin I-containing proteins The most highly ... component of the 4 022 A H Warner et al (Eur J Biochem 27 1) Ó FEBS 20 04 β β β β Fig Comparison of the fasciclin I domains in CLAP with selected proteins in the protein database The Conserved Domain Database...
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Báo cáo khoa học: Structural and functional evidence for a singular repertoire of BMP receptor signal transducing proteins in the lophotrochozoan Crassostrea gigas suggests a shared ancestral BMP/activin pathway docx
Ngày tải lên :
07/03/2014, 21:20
... boxed The C1 and C2 domains appeared to be joined by a ‘linker’ sequence Also boxed are the transmembrane domain and the serine ⁄ threonine kinase domain FEBS Journal 27 2 (20 05) 3 424 –3440 ª 20 05 ... by inserting a stop codon at the C-terminal side of the transmembrane domain This protein included both extracellular C1 and C2 domains as well as the FEBS Journal 27 2 (20 05) 3 424 –3440 ª 20 05 ... 9B3,B4) FEBS Journal 27 2 (20 05) 3 424 –3440 ª 20 05 FEBS The C1 and C2 domains of TGFbsfR2 have different ligand binding properties Although the C1 binding domain of Cg-TGFbsfR2 clustered with its...
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