negative selection with antibodies coupled microbeads 29 30
báo cáo hóa học:" Birth weight and characteristics of endothelial and smooth muscle cell cultures from human umbilical cord vessels" docx
... for citation purposes) Journal of Translational Medicine 2009, 7 :30 http://www.translational-medicine.com/content/7/1 /30 covered with a drop of FA mounting fluid pH 7.2 containing 1.25 μg/mL DAPI ... throughout life, with or without structural changes in the vascular tree The presence of early alterations in vascular function has been described in children and adolescents with low birth weight ... Dis Child 1991, 66: 1291 - 1295 Whincup PH, Cook DG, Papacosta O: Do maternal and intrauterine factors influence blood pressure in childhood? Arch Dis Child 1992, 67:1423-1 429 Lurbe E, Torro MI,...
- 10
- 431
- 0
Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... evergzloH ,D kebruS ,C nessiN ,E aiaksvolkhC ,W nesnaJ ,V eranobraC ellad ,A hcsryW 72 473 -863 ,121 ,300 2 lotameaH J rB ton era doolb tluda dezilibom dna droc lacilibmu tub sllec 'mets' lamyhcnesem ... corP worram eht ot sllec rotinegorp citeiopomeh fo gnimoH JJ lleugniM ,M ilossavaT 42 08-66 ,31 ,299 1 veR rcodnE noitaitnereffid tsalcoetso fo noitaludoM JT nitraM ,N ihsahakaT ,T aduS 32 739-139 ... namuH CM luojenreV eD ,A enneilluJ ,E ertsahC ,P lecrO ,F duahciP ,J nniuQ ,S xuoR 12 011-501 ,12 ,300 2 slleC metS droc lacilibmu morf sllec ekil-CSM etadidnac :sllec mets lamyhcnesem namuh latantsop...
- 6
- 410
- 0
Báo cáo y học: " Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates " pps
... array was incubated with a cocktail of phospho-site-specific biotinylated antibodies for h at room temperature, followed by streptavidin-HRP for 30 Signals were visualized with chemiluminescent ... Furthermore, the mortality rate in E coli-induced ALI mice was about 30% with antibiotics treatment, which is consistent with infection related-ARDS mortality in clinical settings [4] Overall, ... 2007, 109: 3308 -3315 Opitz B, van Laak V, Eitel J, Suttorp N: Innate immune recognition in infectious and noninfectious diseases of the lung Am J Respir Crit Care Med 2010, 181: 1294 - 1309 Ford JW,...
- 11
- 353
- 0
Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs" pot
... spuorg ehT skeew gnirud serocs yblO giF ylevitcepser ,s /m 99.03 ± 46.93 dna s/m 31.62 ± 29. 84 ,s/m 77.73 ± 29. 34 ot pu devorpmi erew skeew ta GBCU dna CSM-BCU ,G ni seiticolev noitcudnoc ehT skeew ... enilas :PC ,tnemtaert on :NC ,ces/m* 99.03 ± 46.93 89.61 ± 44.520 EN GBCU 31.62 ± 29. 84 84.9 ± 45.62 EN CSM -BCU 77.73 ± 29. 34 *07.9 ± 42.22* EN G EN EN EN PC EN EN EN NC spuorG skeeW dna ,0 keew ta ... ,13 ,300 2 lotameH pxE sllec mets lamyhcnesem detaitnereffidnu dna detaitnereffid fo seitreporp cigolonummi dna noisserpxe ALH O nédgniR ,E grebretteZ ,K lhadnesoR ,C kimmaT ,K cnalB eL 61 299 -589...
- 8
- 226
- 0
Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs" ppsx
... implantation Canine UCB-MSCs were suspended with 500 μl of normal saline prior to mixing with 700 mg of β-TCP (group CM) The same volume of normal saline mixed with β-TCP was prepared as the control ... bloc ceramics with pores precultured with cells in osteogenic medium or vacuumed for uniform loading and retention of cells [13,34] In the present study, β-TCP granules were mixed with cells immediately ... Implants with autologous bone marrow MSCs contain more new bones within and around implants in canine segmental bone defect [3] While undifferentiated adipose derived stromal cells with β-TCP...
- 7
- 220
- 0
Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
... were blocked o with normal goat serum overnight at C Cells were o incubated with primary antibodies overnight at C After washing times, the cells were incubated with secondary antibodies Alexa ... were washed with D.W times and fixed in a solution of ice-cold 70% ethanol for h After carefully washing times with D.W, cells were stained for 10 with 40 mM Alizarin red S after washed with D.W ... amplified at 33 to 45 o o o o cycles of 94 C (30 sec), 58 C to 61 C (30 sec), 72 C (1 o min), followed with 72 C for 10 PCR products were visualized with ethidium bromide on a 3% agarose gel 183...
- 7
- 439
- 0
Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx
... minced well in HBSS, and digested with mg/ml collagenase type II (Sigma, St Louis, MO) at 37°C in a water-bath shaker for 30 to 40 minutes at 180 to 200 rpm with a brief vortex every 10 minutes ... localisation of CD44, CD29, CD105 and CD90, but no localisation of haematopoietic markers CD45, CD34 [23] These sand rat-derived AD-MSC are positive for the markers CD29 and CD105 and negative for the ... Lowell, MA) in 24-well plates and fed three times per week with 2.0 ml of MSCBM with 10 ng/ml TGFβ (Cambrex Bio Science, Walkersville, MD) or without TGFβ (control) The typical dose of TGFβ used in...
- 10
- 446
- 0
Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx
... incubated with primary antibodies against human CD29, CD45, CD73, CD105, CD166, HLA-DR (Biolegend, USA) and CD31, CD34, CD44 (BD Biosciences, USA) All antibodies were diluted 1:100 and incubated with ... relocated in a sterilized bottle filled with 0.1 M phosphate-buffered saline (PBS) at 4°C within 24 h prior to use Page of 30 at 37°C The cells were fixed with methanol and acetic acid mixed by ... started, the cells were fixed with 4% paraformaldehyde for 10 at room temperature, and rinsed with PBS The fixed cells were then incubated for hour at 37°C with mouse antibodies against human CD31...
- 9
- 519
- 0
Báo cáo y học: "HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall-derived mesenchymal stem cells" pot
... 0.05 with HIV-1 IIIb , 5.8 ± 1.4 p < 0.05 with HIV-1ada and 4.7 ± 1.3 p < 0.05 with gp120) and δ (3.6 ± 1.2, p < 0.05 with HIV-1 IIIb, 3.4 ± 1.3 p < 0.05 with HIV-1ada and 3.5 ± 0.9 p < 0.05 with ... transcripts (2 ± 0.5 fold increase with HIV-1IIIb; p < 0.05, 1.7 ± 0.3 fold increase with HIV-1ada; p < 0.05 and 1,8 ± 0,4 fold increase with gp120; p < 0.05) with respect to induced cell culture ... activity in comparison with the cell culture control (3.4 ± 0.5 fold increase with HIV-1 IIIb (p < 0.05), ± 0.4 fold increase with HIV-1 ada (p < 0.05) and 2.7 ± 0.5 fold increase with gp120 (p < 0.05)...
- 18
- 247
- 0
Báo cáo sinh học: "Autologous Transplantation of Adipose-Derived Mesenchymal Stem Cells Markedly Reduced Acute Ischemia-Reperfusion Lung Injury in a Rodent Model" potx
... increased pulmonary mRNA expressions of IL-10 and adiponectin in animals with ADMSC therapy compared with those without In concert with the finding of the present study, one previous study has also ... primary antibodies Irrelevant antibodies were used as controls in the current study The extent of crowded area, which was defined as the region of thickened septa in lung parenchyma associated with ... for two hours, followed by incubation with secondary antibody solution (1 :300 ) for one hour at room temperature The washing procedure was repeated eight times within 40 minutes Immunoreactive bands...
- 13
- 280
- 0
Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx
... suspension and incubated at 37°C for minutes, then at 4°C for 15 minutes with occasional mixing MSCs labeled with DiI were washed times with PBS and then collected Laser Doppler blood perfusion analysis ... were then incubated with primary antibodies including VEGF (1:1000, Cell Signaling) and b-actin (1:5000, Sigma) at 4° C overnight respectively.The membranes were then incubated with peroxidase labeled ... every days for 15 passages without visible morphologic change Flow cytometry examination showed that these cells were negative for CD34 and CD45, but positive for CD44 and CD29 (Fig 1) Thus, we designated...
- 10
- 230
- 0
Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates
... [56], Copyright 2009, with permission from John Wiley & Sons Inc) Table 2.8: Electrospun Nanofibrous Composites with Calcium Salts (Reprinted from [56], Copyright 2009, with permission from John ... 2005, with permission from Elsevier Limited) Fig 2.5: A typical tissue-engineered material construct, with the addition of cells and growth factors (Reprinted from [56], Copyright 2009, with permission ... (p
- 241
- 446
- 0
Báo cáo sinh học: " Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia'''' docx
... T Cells (%) 30 CD3+CD4 (%) CD3+CD8 (%) Th/Tc (%) Pretreatment Posttreatment P value 78 .29 ± 8.011 74.85 ± 8.588 0.002 30 49.07 ± 8.531 44.93 ± 9.642 50% and 17 /30 showed improvement between 5% ~ 49% The highest increase was 87.5% ... tandem with advances in genetic testing that define the different types and the locus of genotype variation, abnormalities within chromosomes and proteins Mutational analysis can correlate with...
- 5
- 324
- 0
báo cáo hóa học:" Human umbilical cord blood-derived mononuclear cell transplantation: case series of 30 subjects with Hereditary Ataxia" pdf
... T Cells (%) 30 CD3+CD4 (%) CD3+CD8 (%) Th/Tc (%) Pretreatment Posttreatment P value 78 .29 ± 8.011 74.85 ± 8.588 0.002 30 49.07 ± 8.531 44.93 ± 9.642 50% and 17 /30 showed improvement between 5% ~ 49% The highest increase was 87.5% ... tandem with advances in genetic testing that define the different types and the locus of genotype variation, abnormalities within chromosomes and proteins Mutational analysis can correlate with...
- 5
- 472
- 0
Báo cáo y học: "Sphingosine-1-phosphate promotes the differentiation of human umbilical cord mesenchymal stem cells into cardiomyocytes under the designated culturing conditions" pdf
... or with conditional culturing combined with S1P As demonstrated in Figure 1, after induction with conditioned culturing, the cells began to change their morphology with time In cells treated with ... morphology and immunostaining with cardiac myocyte specific markers Immunocytochemistry The medium was first removed and the cells were washed twice with PBS, fixed for 30 with 4% paraformaldehyde ... *P < 0.05 compared with DMEM-only group and day; *P < 0.05 compared with days B statistical difference at *P < 0.05 compared with DMEM-only group and day; *P < 0.05 compared with days indicate...
- 9
- 262
- 0
Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps
... mice treated with UC-MSCs or not were intradermally injected with 10 μg CII/10 μl PBS in the right ear and with 10 μl PBS in the left ear Ear swelling was measured 48 hours later with a spring-loaded ... were treated with osteogenic medium for three weeks with medium changes twice weekly Osteogenesis was assessed at weekly intervals Osteogenic medium consists of MEM-a supplemented with 0.1 μM ... from RA patients Synovial tissues were obtained from patients with RA (n = 5, females, aged from 30 to 60 years) and traumatic patients without arthritis (n = 4) at time of knee replacement surgery...
- 13
- 297
- 0
Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model
... The work of this thesis has been done in several laboratories, with the support of many people I feel lucky to have known and worked with these people and I would like to express my gratitude here ... students and colleagues that I have worked with on this thesis project It is impossible to name them all here But I feel especially grateful to Lin Gen who worked with me on the immunological experiments ... first few drafts of this thesis I benefited a lot from working with Phua Meow Ling, Cindy on the diabetic mouse model and from discussion with Dr Laura Rivino I would also like to thank other people...
- 226
- 362
- 0
Tài liệu Báo cáo khoa học: Binding of N- and C-terminal anti-prion protein antibodies generates distinct phenotypes of cellular prion proteins (PrPC) obtained from human, sheep, cattle and mouse doc
... (SE 1.5; 1.1; 0.5); 30 gels with antibody P4 (SE 0.9; 0.7; 1.2); five gels with antibody 6H4 (SE 4.6; 3.9; 4.7); seven gels with antibody SAF60 (SE 1.1; 1.0; 1.4); 30 gels with antibody SAF70 ... revealed high signal intensities with antibodies SAF60, SAF70 and 6H4 compared with full-length PrPC (Fig 3C) Observations similar to human PrPC were also observed with PrPC from cattle, sheep and ... were found with antibodies binding to the central region of PrPC, 1494 as the signal intensities at the size of the nonglycosylated full-length PrPC (at 27 kDa) were high with monoclonal antibodies...
- 11
- 536
- 0
Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf
... or commercial antibodies to actin and NRF2 (1 : 1000 dilution) in TBST containing 5% BSA The membrane was washed for 15 with TBST and incubated with goat anti-mouse IgG conjugated with horseradish ... Bach1 competes with Nrf2 leading to negative regulation of the antioxidant response element (ARE)-mediated NAD(P)H:quinone oxidoreductase Transcription of noncoding DNA 25 26 27 28 29 30 31 32 33 ... size markers The expected size of the product with ASAT primers is 132 bp and with S3T primers )160 bp (B, right section) Kinetics of amplification with ASAT primers of identical amounts of cDNA...
- 11
- 463
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 chỉ tiêu chất lượng 9 tr 25