naming in normal aging and dementia of the alzheimer apos s type
Báo cáo khoa học: In vitro gamma-secretase cleavage of the Alzheimer’s amyloid precursor protein correlates to a subset of presenilin complexes and is inhibited by zinc potx
... IC50 values of 22 and lm Zn Using two assay systems, these results show that zinc can inhibit in vitro c-secretase cleavage of an APP substrate Discussion The amyloid hypothesis of AD states that ... overexpress the c-secretase complex components These assays are used to test the effects of detergent concentration, solvents and metals on the c-secretase cleavage of APP substrates These studies show ... discovering c-secretase inhibitors is the development of assays that monitor c-secretase activity This paper describes two novel in vitro c-secretase assays During the development of these assays...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... corresponding to the wild -type laccase from P cinnabarinus Immunodetection of the laccase was performed using antibodies raised against the P cinnabarinus laccase The Western blot analysis showed ... approach, and the cloned PCR products were checked by sequencing Table shows the primers, vectors, and restriction sites used in the cloning strategy, and Table lists the primer sequences Constructs ... Glycosylation was 0.5% of the molecular mass of the native laccase and and 10% for the recombinant laccase In the heterologous production of the P cinnabarinus laccase in P pastoris [14] or the...
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Báo cáo khoa học: "Distribution of trkA in cerebral cortex and diencephalon of the mongolian gerbil after birth" docx
... diaminobenzidine and 0.05% H2O2 in PBS) After staining the sections were mounted on silane-coated slides (3-amino propyltriethoxy silane, Sigma, USA) Results We only describe those areas in which ... to synthesize NGF TrkA in hippocampus has been shown to increase progressively after birth and peak at P21 [15] Thus it appears that expression of NGF in the target-fields of the NGF-responsive ... 100 in PBS at 4oC for 12-24 hours Sections were immunostained using a standard biotine-avidin detection system (Vectastain, USA) Visualization of immunobinding was carried out with DAB solution...
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Báo cáo khoa học: "Characterization and localization of the unique Marek''''s disease virus type 2 ORF873 gene product" ppsx
... Buckmaster AE, Scott SD, Sanderson MJ, Boursnell MEG, Ross LJN, Binns MM Gene sequence and mapping data from Marek s disease virus and herpesvirus of turkeys: implication for herpesvirus classification ... three MDV serotypes In this communication, it is described that the unique MDV2 ORF873 protein is being expressed and localized in cytoplasm of the virus-infected cells as the molecular mass of a ... nature of the virus The goles in the present and previous studies [8-10,14,18] have been to dissect the genomic structure and to understand the functions of the different viral proteins among the...
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Báo cáo y học: "Mirror-Image Arachnoid Cysts in a Pair of Monozygotic Twins: A Case Report and Review of the Literature"
... by themselves However, we have only found one such case of MZ with mirror-imaging of AC in the literature In this case, we describe the second case of MZ with mirror-imaging of AC and discuss the ... important in the predisposition to cystogenesis in some patients Previously, both the familial occurrence of AC and the presence of AC in patients with genetically determined cystic diseases, such as ... wall These finding indicated NKCC1 gene might play an important role in cystogenesis The association of AC and other diseases in families suggested that PAPB2, SPG4 and SOX2 gene might be linked...
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Báo cáo y học: "Aplasia and Agenesis of the Frontal Sinus in Turkish Individuals: A Retrospective Study Using Dental Volumetric Tomograph"
... frontal sinuses [1] CT scans of agenetic patients show almost normal frontal recesses and sinuses, although there is only one frontal sinus ostium The size of the sinus and, therefore, its anatomic ... Occasionally, one or both sinuses may be absent The prominence of supercilliary arcs does not indicate the absence, presence or size of the frontal sinus [3] The objective of this study was to investigate ... with a smooth superior margin and an absence of the sinus septa [20] http://www.medsci.org Int J Med Sci 2011, 280 Table Frequency of frontal sinus agenesis Discussion Sex The variations in the...
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Đo kiểm đánh giá can nhiễu mạng truyền hình cáp The Measurement and Analysis of the effect of interference in Television Cable network
... generation framing structure, channel coding and modulation systems for Broadcasting, Interactive Services, News Gathering and other broadband satellite applications (DVB – S2 ) 14 Tim Williams – EMC ... CCIR and FFC tv standards – Printed in the federal republic of Germany 18 3GPP2 C .S0 011-C, version 2.0 – Recommended Minimum Performance Standards for cdma2000 Spread Spectrum Mobile Stations ... Designers – Newnes Editor –Fourth Edition 15 V.Prasad Koladi – Engineering Electromagnetic Compatibility: Principles, Measurements and Technologies -1996 IEEE Press 16 Walter Ciciora, James Farmer,...
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Tài liệu Báo cáo khoa học: Interaction between very-KIND Ras guanine exchange factor and microtubule-associated protein 2, and its role in dendrite growth – structure and function of the second kinase noncatalytic C-lobe domain docx
... 1B) The results obtained indicate that the KIND2 domain is necessary and sufficient for the targeting of v-KIND to dendrites of neurons To investigate the MAP2-binding characteristics of the KIND ... conserved in the KIND2 of v-KIND in all species analyzed, although not in the other KINDs To investigate the possible involvement of these conserved Leu residues in the interaction between v-KIND ... v-KIND KIND2 binding resides within residues 702–744 of MAP2 Residues 456–487 in the KIND2 domain of v-KIND contain the core MAP2 binding module To identify the core MAP2-binding site within the...
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Tài liệu Báo cáo khoa học: Identification and characterization of the transcription factors involved in T-cell development, t-bet, stat6 and foxp3, within the zebrafish, Danio rerio docx
... variety of tissues and cells in the Ginbuna crucian carp [27] and, in mammals, in the lung tissue of mouse and spleen and thymus of human and mouse [11,37] Foxp3 is also highly expressed in lymphoid ... and skin tissue of healthy fish (data not shown) Previous studies on the expression of these genes within humans and mice, of T-bet within Ginbuna crucian carp [27] and STAT6 in mandarin fish [28] ... characterized in this investigation, as they are either involved in mammalian Th1, Th2 or Treg responses or are good markers of an antiviral response 10 11 Statistical analysis The assessment of statistical...
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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
... opposite effect To address this question, we first assessed the ability of insulin administered in vivo to increase the level of RCAM-lysozyme in cell fractions, and then assayed the fractions of ... overexpression of Grb14 in CHO-IR cells impairs Akt and ERK insulin signaling pathways and inhibits distal effects of insulin on glycogen and DNA synthesis [6–9], and microinjection of Grb14 into ... have shown that Grb14 binds as a pseudosubstrate inhibitor to the peptide-binding groove of the kinase and thus acts as a selective inhibitor of insulin signaling Consistent with this observation,...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... synthesis Nevertheless, this decrease was lesser by comparison to the one observed in the presence of FSH and bFGF ()49%) This result suggested that bFGF action could induce, in part, a Statistical ... biological signi®cance of the different regulation of their expression As a ®rst element step towards understanding, a recent demonstration indicated that the synthesis of cell surface HSPG and FSH-stimulated ... partners bFGF inhibits the FSH-stimulated estradiol synthesis in Sertoli cells Sertoli cells from 20-days-old-rats were incubated for 24 h with FSH (100 ngámL)1) and increasing concentrations of...
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Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... proteins (UspA family) As shown previously [12,14,17], the synthesis of UspA and three of its paralogues is greatly increased under various stress conditions that cause the arrest of growth In ... requires the assistance of two chaperone systems The identification of UP12 as a putative in vivo substrate of GroEL was interesting, because this protein belongs to a family of universal stress proteins ... previously shown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably, but only transiently [2,18] Similarly, we observed that the steady-state...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... antibody was raised against a sequence between the N-terminal and the first transmembrane domain of the protein of the human and rat CB2 receptor The specificity of the CB1 and CB2 antibodies was described ... hydrolase (FAAH), we used two independent methods RT-PCR was used to determine the presence of the messenger RNAs, and Western immunoblot analysis was used to determine the presence of the corresponding ... supported by the observation that preabsorbing of the CB1 antibody with its corresponding blocking peptide eliminated almost all of the staining of these bands (Fig 3A) The most abundant band in human...
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Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... shown) Therefore, it appears that Spatzle-C108 signal¨ ing may stimulate expression of a subset of the genes whose expression is induced by microbial exposure in M sexta Discussion Progress in ... The mixtures were subjected to SDS-PAGE and immunoblotting using Spatzle antibodies The ¨ ¨ sizes and positions of molecular weight standards are indicated on the left Bands representing proSpatzle ... which is most similar to Drosophila SPE and Easter [31] Injection of either of these M sexta proteinases into larvae stimulated the expression of antimicrobial peptide genes, suggesting that they...
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Gentle Measures in the Management and Training of the Young docx
... were the scenes of his infantile occupations and joys, and sees the now useless playthings and books, and the various objects of curiosity and interest with which he was so often and so busily ... goodness devises for the benefit of the future Theologians have lost themselves in endless speculations on the question how far, in the government of God, punishment is to be considered as possessing ... Mary 's suspicions are awakened and children habitually managed on these principles soon learn to be extremely distrustful and suspicious and she insists on going into the house, and thus discovers...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... membranes After successful import, the transit peptide is cleaved off by the stromal processing peptidase (SPP), resulting in the mature form of the protein The entire process is superficially reminiscent ... translocation steps happening on both sides of the IEM This includes the assembly of Toc–Tic ‘supercomplexes’ [7–9], preprotein recognition [10], translocation, and folding steps of Translocation across the ... domain and a soluble C-terminus protruding into the stroma Conserved regions of the C-terminus are the TPR domain, consisting of seven predicted a-helices (blue), and the Sti1-like Hip/Hop domain...
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Báo cáo khoa học: Eukaryotic class 1 translation termination factor eRF1 ) the NMR structure and dynamics of the middle domain involved in triggering ribosome-dependent peptidyl-tRNA hydrolysis pptx
... chemical shifts of signals from the vast majority of the residues of the M domain (Fig 1B) This is strong evidence that there is no substantial change in the conformation of the protein or in the distribution ... domains may have structural in uences on the M domain within the whole eRF1 protein The pairwise comparison of the solution structures with the X-ray crystal structure of the M domain using the superposition ... values of the internal motion correlation times sfast were between and 20 ps The alignment of the amino acid sequences of the M domains of eRF 1s and aRF 1s (archaeal RFs) from diverse organisms, including...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt
... Vasconcellea spp.: (1) a higher protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these two hypotheses, the protein concentration ... pGEM-T plasmids (Promega Benelux, Leiden, The Netherlands) following the manufacturer s instructions Insert sequencing was carried out in both directions using T7 and SP6 primers Inserts were sequenced ... N-terminus of papain-like cysteine proteinases, leaving no doubt that these proteins belong to the papain superfamily Sequencing of VXH-I and VXH-III yielded two signals of equal intensity at positions...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
... to the right Conserved amino acid residues forming the active site of the A-chain are marked with asterisks [27] The residues participating in galactose binding in the 1a and 2c subdomains of the ... modifications cause the differences between the proteins [15] The present work describes the full-size encoding sequences of the mistletoe toxic lectin genes Analysis of the primary structure of the ... very similar pattern of five bands, one of which was also more intensive than the others Thus, the different intensity of the bands may be the result of a different copy number of the genes in the...
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Spanishness in the Spanish Novel and Cinema of the 20th – 21st Century docx
... Spanishness in the Spanish Novel and Cinema of the 20th – 21st Century Edited by Cristina S nchez-Conejero CAMBRIDGE SCHOLARS PUBLISHING Spanishness in the Spanish Novel and Cinema of the ... any SPANISHNESS IN THE SPANISH NOVEL AND CINEMA OF THE 20TH-21ST CENTURY individual who is a native speaker of Spanish or is of Spanish descent— including Hispanic-Americans As a consequence, the ... racism in Spain, whether conscious or not, and thus racism must be considered as part of the social fabric of Spanish identity, as discoursed in the 199 0s by such musical acts as Amistades Peligrosas...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008