một số vấn đề lý luận về vi phạm pháp luật
Báo cáo y học: "JNK suppression is essential for 17b-Estradiol inhibits prostaglandin E2-Induced uPA and MMP9 expressions and cell migration in human LoVo colon cancer cells" ppsx
Ngày tải lên :
10/08/2014, 10:20
... purchased from TOCRIS (Ellisville, Missouri, USA) 6-Amino-4-(4-phenoxyphenylethylamino) quinazoline (QNZ), NFB activation inhibitor was purchased from Peptides International (Louisville, Kentucky, USA) ... AdTIMP-2treated mice showed a significantly prolonged survival [31], which emphasizes the importance of endogenous regulation of MMPs activity by TIMPs In the present study, we further detected ... extracellular matrix Author details Division of Colorectal Surgery, Mackay Memorial Hospital, Taipei, Taiwan Mackay Medicine, Nursing and Management College, Taipei, Taiwan Division of Cardiology, Taipei...
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Báo cáo khoa học: Expression of heme oxygenase-1 is repressed by interferon-c and induced by hypoxia in human retinal pigment epithelial cells pot
Ngày tải lên :
23/03/2014, 13:20
... the occurrence of lung metastasis [53] These results provide the in vivo evidence suggesting the beneficial role of the reduced HO-1 activity To explore the physiological significance of the reduced ... RPE cell lines, ARPE-19 [37] and D407 [38], were provided by L M Hjelmeland (Department of Biological Chemistry, University of California, Davis, CA, USA) and R C Hunt (Department of Microbiology, ... containing 10% fetal bovine serum, mM L-glutamine, and antibiotics (100 UÆmL)1 penicillin and 0.1 mgÆmL)1 streptomycin) [37] D407 cells were cultured in DMEM containing 10% fetal bovine serum, mM L-glutamine...
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Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 04:20
... (Gibco-BRL, Rockville, MD, USA) The culture medium containing the recombinant virus generated by homologous recombination was collected days after transfection The titers of recombinant viruses were ... 1C), suggesting that the processing activity of MIG-17 is specifically upregulated during L3 and L4, when DTCs actively migrate Consistent with our in vitro experiments [17], when MIG-17–GFP with ... distributions of these proteins remain to be investigated This article presents the first evidence of in vivo activation of an ADAMTS protein that is essential for correct organ morphogenesis Monoclonal...
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Báo cáo khoa học: Cas utilizes Nck2 to activate Cdc42 and regulate cell polarization during cell migration in response to wound healing docx
Ngày tải lên :
23/03/2014, 03:20
... experiments were performed to measure Cdc42 activity in the absence of Nck2 and the graph indicates the relative activity of Cdc42 (mean ± SD) biological activities, including actin cytoskeleton reorganization ... transduction pathways J Cell Sci 118, 2579–2587 38 Osmani N, Vitale N, Borg JP & Etienne-Manneville S (2006) Scrib controls Cdc42 localization and activity to promote cell polarization during astrocyte ... with Cas siRNA exhibited approximately half of the Cdc42 activity found in control transfectants h after wounding Cdc42 was also evident at the ends of cell protrusions on the wound edge in control...
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Báo cáo Y học: Bax-induced cell death in yeast depends on mitochondrial lipid oxidation doc
Ngày tải lên :
23/03/2014, 21:20
... A titration curve of the activity was first built with commercial bovine erythrocytes SOD so that the activity of SOD in proteic extract could be calculated SOD activity was also qualitatively ... plates with a solution of 0.001% primuline in 80% acetone and vizualized under UV light The silica gel zones corresponding to individual lipids were scrapped and methyl ester fatty acids were ... same buffer containing 28 mM TEMED and 28 lM riboflavine After a brief washing in water, the gel was revealed on a light table Catalase activity was measured on the same proteic extracts Proteins...
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Báo cáo khoa học: Activation of p21-activated kinase 1 is required for lysophosphatidic acid-induced focal adhesion kinase phosphorylation and cell motility in human melanoma A2058 cells potx
Ngày tải lên :
30/03/2014, 13:20
... recruits other proteins, including paxillin, p130Cas, vinculin and talin in focal adhesions These protein complexes anchor the actin cytoskeleton and provide structural integrity to cells [16] The importance ... PAK and FAK in cell motility and LPA-induced FAK activation via RhoA or Rho-associated kinase (ROCK) [19], there has been no direct evidence showing the roles of PAK activation on LPA-induced ... line A2058 was maintained as described previously [20] The cells were maintained at 37 °C in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum in a humidified 5% CO2 For analysis...
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cell migration in inflammation and immunity
Ngày tải lên :
10/04/2014, 11:13
... fiocs and transfer to vials (fraction C) 13 Transfer the double filter system to vials together with the medium of the lower compartment (fraction D) 14 Measure radioactivity in each fraction ... magnification, an area of 0.2 mm2 is visualized at video; phase-contrast optics improves the image quality The syringe is placed on the digital pump Set the digital pump to provide a flow rate of 75 mL/h, ... activity were dialyzed against 50 mM formate, pH 4.0, and loaded on a Mono S column Proteins were eluted in a linear NaCl gradient and MCP-1 immunoreactivity was measured with a RIA (B) The activity...
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Báo cáo y học: " Vasoactive intestinal peptide inhibits TNF-α-induced apoptotic events in acinar cells from nonobese diabetic mice submandibular glands" docx
Ngày tải lên :
09/08/2014, 14:20
... & Therapy Vol 11 No Calafat et al ease activity Evidence obtained with the nonobese diabetic (NOD) Sjögren's mouse model also supports this view providing a feasible approach for this search ... previously been reported [16] Vasoactive intestinal peptide (VIP) is a neuroimmunopeptide with several actions on exocrine glands In addition to inducing vasodilation and exocrine secretion, VIP ... syndrome-like stage, treatment with VIP in vivo from the fourth week of age reduced Th1 cytokine levels in the serum and increased IL-10 [21] In line with this, VIP was proposed as one of the promising...
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Báo cáo y học: "Hypoxia upregulates angiogenesis and synovial cell migration in rheumatoid arthritis" docx
Ngày tải lên :
09/08/2014, 14:20
... tenosynovium was significantly more hypoxic (median oxygen tension 3%, 26 mmHg) than either noninvasive tenosynovium or joint synovium in the same RA patients, suggesting that hypoxia might be driving ... balance in RA, and the in vivo relevance of such changes to synovial cell migration, however, have not been investigated Previous studies have demonstrated that RA tenosynovial cultures, obtained ... encapsulating tenosynovium [21,22] RA tenosynovium was subsequently reported more vascular (assessed by measuring CD31 expression) than RA joint synovial lining [23], although the driving force behind...
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Báo cáo y học: "Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo" doc
Ngày tải lên :
13/08/2014, 15:21
... study and revised the manuscript ESMC and LL conducted the in vivo experiments WKL designed the in vivo experiment and prepared the human colon cancer cells CMNY designed the in vitro experiment ... HT-29 cell lines grown in vitro and compared those results with its effect on tumor growth in vivo We found that TXL attenuated the growth of xenografted HT-29 tumors in vivo The injection of 5-fluorouracil ... apoptosis in colon cancer via the mitochondrial pathway The above concomitant molecular events in TXLtreated HT-29 cells result in remarkable apoptosis process Further in vivo and in vitro studies are...
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Báo cáo y học: "Directed cell migration in the presence of obstacles" doc
Ngày tải lên :
13/08/2014, 16:21
... of chemotactic behavior have been studied from both an experimental and a theoretical point of view (e.g [3-9]) In this article we study the efficiency of chemotaxis in achieving controlled cell ... requires an approach involving an individual-based model (IBM) of cell movement In this article we construct a minimal IBM of chemotactic cell movement in an obstacle-ridden environment Our aim is ... of a multi-obstacle environment This leads to an expression for the distance over which chemotaxis is viable as a means of directing cells from one point to another in vivo Chemotactic motion...
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Genetic analysis of the role of ARP2 3 complex in border cell migration in drosophila melanogaster
Ngày tải lên :
10/09/2015, 15:48
... cell culture studies However, there are obvious differences between in vitro and in vivo cell migrations Cells crawl on substratum in a 2-D pattern in vitro, as one side contacts the substratum ... NPFs and Arp2/3 have been elusive Although cofilin promote debranching in vitro, there is no supporting evidence of this in vivo (Blanchoin et al 2000) Recently, two negative regulators of Arp2/3, ... control of movies All the best for her future academic career Thank Dr Inaki Mikiko for sharing of stocks and movies on cytochalasin-D drug treated border cells Thank Dr Cliffe and Dr Smitha Vishnu...
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Cancer cell migration in 3d collagen gel matrix system
Ngày tải lên :
12/09/2015, 09:22
... collagen model reconstituted in vitro Chapter provides the literature review of current in vitro 2D and 3D cancer metastasis assays followed by a summary of in vitro 3D anti-cancer drug assays ... surfaces, which inevitably have several drawbacks Although convenient to set up, 2D cultures cannot reproduce geometrically the environments that house cells in vivo, nor provide cells with cues ... Review Chapter 2: Literature Review In the first part of this chapter, we will review the commonly used techniques that scientists employed to study cell migration on 2D surfaces or in 3D environments,...
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CHARACTERISATION OF DRUG INDUCED CELL DEATH IN MYCOBACTERIUM SMEGMATIS
Ngày tải lên :
02/10/2015, 17:15
... Aerobic organisms require oxygen for respiration and driving their cellular activity; unlike anaerobes that fail to survive in oxygenated environments However hyperoxia, a condition of excess oxygen, ... to live and proliferate Food, space and environment play an important role in survival Even oxygen, a life nurturing chemical could be toxic for survival While anaerobes cease to thrive in the ... just being there ii TABLE OF CONTENTS ABSTRACT v LIST OF TABLES vi LIST OF FIGURES vii INTRODUCTION 1.1 CELLULAR ENVIRONMENT INDUCED DEATH 1.2 ANTIBIOTIC INDUCED CELL DEATH 1.3 THE COMMON DEATH PATHWAY-...
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Tài liệu Báo cáo khoa học: Staphylococcal enterotoxin C1-induced pyrogenic cytokine production in human peripheral blood mononuclear cells is mediated by NADPH oxidase and nuclear factor-kappa B doc
Ngày tải lên :
19/02/2014, 00:20
... SEC1 induces pyrogenicity via NADPH oxidase 3637 SEC1 induces pyrogenicity via NADPH oxidase C.-L Su et al nuclear translocation (Fig 2C, lanes 5–7) The DNAbinding activity of NF-jB induced by ... scavenging activity by nonsteroidal anti-inflammatory drugs Life Sci 76, 2841– 2848 68 Leo R, Pratico D, Iuliano L, Pulcinelli FM, Ghiselli A, Pignatelli P, Colavita AR, FitzGerald GA & Violi F (1997) ... The NF-jB-DNA binding activity (lanes 2–8) was quantified by densitometry The time-course groups were compared with the Ctl group to obtain the relative binding activity (C) Western blot analysis...
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Báo cáo khoa học: Identification of carbonic anhydrase 9 as a contributor to pingyangmycin-induced drug resistance in human tongue cancer cells ppt
Ngày tải lên :
06/03/2014, 22:21
... were then transferred to a poly(vinylidene difluoride) membrane, and immunoblotting 4516 Caspase activity assay Caspase activity was determined with a caspase activity kit (Beyotime), through cleavage ... antiapoptotic activity of Tca8113 ⁄ PYM cells to enhance the PYM effect To further observe the enhanced effect of CA9 interference on PYM-induced Tca8113 ⁄ PYM apoptosis, caspase activity was investigated ... PYM, P < 0.01 (C, D) CA9 ASO significantly decreased cell viability, with a marked reduction in IC50 value, and increased the apoptotic activity induced by PYM on Tca8113 ⁄ PYM cells (E) Western...
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Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for amplification of mitochondrial apoptotic cell death in human cervical cancer cells pptx
Ngày tải lên :
07/03/2014, 05:20
... of MAP kinases activated primarily by cytokines and exposure to environmental stress [19,20] Numerous reports have provided evidence that JNK can function as a pro-apoptotic kinase in response ... cervical cancer cells We suggest that ionizing radiation utilizes the JNK signaling pathway to amplify mitochondrial dysfunction and subsequent apoptotic cell death Many reports have provided evidence ... human cervical carcinoma cell line (HeLa) was obtained from the American Type Culture Collection (Rockville, MD, USA) Cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum...
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Báo cáo Y học: Requirement of caspase and p38MAPK activation in zinc-induced apoptosis in human leukemia HL-60 cells potx
Ngày tải lên :
23/03/2014, 21:20
... (1984) Release of endogenous Zn2+ from brain tissue during activity Nature 308, 734–736 17 Charton, G., Rovira, C., Ben-Ari, Y & Leviel, V (1985) Spontaneous and evoked release of endogenous Zn2+ ... transferred to the apoptotic apparatus via various signal transduction pathways [53,54] Recent studies have suggested that apoptotic stimuli are transferred to caspases via a MAPK cascade such as p38MAPK ... results suggest that labile Zn released from Zn pools is a potent regulator of apoptosis via regulation of the activities of caspases It has been reported that execution of caspase activation is preceded...
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Báo cáo Y học: SF2/ASF protein inhibits camptothecin-induced DNA cleavage by human topoisomerase I potx
Ngày tải lên :
24/03/2014, 03:21
... (b) loss of sensitivity to camptothecin for htopo I, or (c) a direct effect of SF2/ASF on the reaction catalysed by htopo I We excluded first two possibilities and provided evidence pointing to ... gels Relaxation activity was calculated from decrease of the amount of supercoiled DNA One unit relaxed 50% of the substrate DNA after 30 at 30 °C Kinase assay Htopo I kinase activity was measured ... drug-sensitivity for the enzyme remaining in the complex with SF2/ASF Such an effect, appearing as an elevated concentration of camptothecin needed to induce DNA cleavage, has been previously observed...
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