mφ with a single tracer
Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
Ngày tải lên :
23/03/2014, 09:21
... graded transactivation potential Nucleic Acids Res 25, 2723–2729 13 Akagi K, Kanai M, Saya H, Kozu T & Berns A (2001) A novel tetracycline-dependent transactivator with E2F4 transcriptional activation ... CGGGCCCGGGATCCATC gccacc ATGG TGA; the 3¢ interface is CAAGTAAA GCGGCCGC For pEBTetD ⁄ eGFP, the 5¢ interface is identical; the 3¢ interface is CAAGTAAA GCGGCCGCGG Cell culture, transfection, and flow ... and with a GAPDH probe (cf Fig 2) Relative background transcription was calculated from the ratios of signals for transporter mRNA and GAPDH mRNA With OCT1, OCT2, and CAT4 both vectors were analyzed...
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Báo cáo Y học: LRP130, a protein containing nine pentatricopeptide repeat motifs, interacts with a single-stranded cytosine-rich sequence of mouse hypervariable minisatellite Pc-1 docx
Ngày tải lên :
31/03/2014, 23:20
... respectively a- tubulin was used as a marker for cytoplasmic extraction The same membrane was used for immunoblot analysis with aF-N, aF-C and anti- (a- tubulin) Ig Lanes and 4, whole cell lysate; lanes and ... accordance with the embryonal develop- Nuclear and cytoplasmic fractions were prepared from NIH3T3 cells and analyzed by immunoblot analysis As shown in Fig 3A, a major band with a molecular mass ... and the first strand cDNAs were synthesized with avian myeblastoma virus (AMV) reverse transcriptase (Takara Biomedicals) at 45 °C for 60 Partial cDNA fragments for the mLRP130 were amplified with...
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Báo cáo y học: "Identification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti-Sm antibodies" ppsx
Ngày tải lên :
09/08/2014, 06:22
... with an offset of two amino acids (13 amino acid overlap) Each arginine-containing peptide was synthesized as three variants, one with natural arginine, one with sDMA and one with asymmetrical ... dimethylated arginine residues for epitopes assay Assay performance characteristics of the anti-SmD3 peptide (SMP) assay (a) Intra-assay and interassay variability, (b) linearity, and (c) receiver operating ... 41.3 and 13.9 units, respectively R24 Correlation with racial and clinical parameters When correlating autoantibody specificities with race, there was a statistically significant association of autoantibodies...
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Báo cáo y học: "Phase delaying the human circadian clock with a single light pulse and moderate delay of the sleep/dark episode: no influence of iris color" potx
Ngày tải lên :
10/08/2014, 09:20
... African Americans had larger phase advances than Caucasians Because we found that African Americans had a shorter tau than Caucasians, which would augment phase advances relative to the Caucasians ... manuscript we also re-analyzed data from our previous phase-advancing study with daily light pulses [42] that included both light and dark-eyed Caucasians as well as dark-eyed African Americans, and thus ... circadian adaptation J Biol Rhythms 1997, 12:5-15 Yang CM, Spielman AJ, D'Ambrosio P, Serizawa S, Nunes J, Birnbaum J: A single dose of melatonin prevents the phase delay associated with a delayed...
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application of real coded genetic algorithm for ship hull surface fitting with a single non-uniform b-spline surface
Ngày tải lên :
13/11/2014, 08:18
... Chiyokura and Kimura (1984) As a result, boundary interpolating patch has several drawbacks for modification a large number of small surfaces that are bonded together to create a larger surface 2.2 ... rectangular patches and triangular patches A flexible CAD system should perform a good quality of shape and an interactive manipulation The B-spline vertex point modification actually works with ... tolerance Fitting process is also expected that the surface is obtained in a reasonable way with high quality of shape Many previous researches approached to automated B-spline surface modeling mathematically...
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Narrowband photon pairs from a cold atomic vapour for interfacing with a single atom
Ngày tải lên :
09/09/2015, 08:15
... work with him and Sandako while doing HOM measurements Gleb, for always teasing me I still miss that Dzmitry, for his great ideas One can approach him anytime and any day and he is always ready ... available in the market 87 Rb is a naturally occurring isotope of Rubidium with atomic number 37 It has a natural abundance of 28%, mass of 86.9 amu with a nuclear spin I of 3/2 Rubidium has another ... with relevant transitions used for cooling the atoms is indicated (Right) Magneto-Optical Trap set up: a glass cuvette attached to a vacuum chamber, quadruple coils and circular polarized beams...
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Comparison of the obstetric anesthesia activity index with total delivery numbers as a single denominator of workload demand in Israeli maternity units doc
Ngày tải lên :
05/03/2014, 15:20
... anesthesia activity index (OAAI) The majority of anesthesia workload in the labor ward comprises epidural labor analgesia and cesarean delivery The OAAI is a formula composite comprising data ... cesarean anesthesia to the total OAAI in individual hospitals Data for 2005 (left) and 2007 (right) The OAAI correlated with epidural rates and cesarean rates more closely than did the annual ... the OAAI (OAAI EPI and OAAI CD) was also calculated as follows: OAAICD/EPI = ( no of cesareans per yr *1.5 ) / ( no of epidurals per yr *0.75) (2) Statistical methods Quantitative variables are...
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Báo cáo khoa học: Biosynthesis of platelet glycoprotein V expressed as a single subunit or in association with GPIb-IX doc
Ngày tải lên :
23/03/2014, 13:20
... Immunoprecipitation and SDS/PAGE Materials and cell lines The mAbs V.1 and V.5 against GPV, ALMA.12 against GPIba, ALMA.16 against GPIX and RAM.1 against GPIbb, were developed in our laboratory [19] ... mass forms Parallel analysis of the supernatants revealed a positive signal starting at 60 of chase and having a molecular mass (82 kDa) consistent with a fully mature form This band was not observed ... Strassel, C., Baas, M.J., Salamero, J., ChasserotGolaz, S., Cazenave, J.P., De La Salle, C & Lanza, F (2001) Biosynthesis and intracellular post-translational processing of normal and mutant platelet...
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Báo cáo khoa học: Solution structure of an M-1 conotoxin with a novel disulfide linkage pdf
Ngày tải lên :
30/03/2014, 09:20
... zation of a novel conus peptide with apparent antinociceptive activity J Biol Chem 275, 32391–32397 18 Sharpe IA, Gehrmann J, Loughnan ML, Thomas L, Adams DA, Atkins A, Palant E, Craik DJ, Adams DJ, ... outcome was a set of 20 ˚ structures with a mean global rmsd of 0.56 ± 0.16 A and a ˚ mean global heavy atom rmsd of 1.30 ± 0.28 A Structural refinement was carried out using amber and structure quality ... resonance of 4,4-dimethyl-4-silapentane-1-sulfonic acid used as an internal standard Distance restraints and structure calculations An initial survey of distance constraints was performed on a series...
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báo cáo hóa học: " Psychometric properties of a single-item scale to assess sleep quality among individuals with fibromyalgia" potx
Ngày tải lên :
18/06/2014, 18:20
... clinical trials During the baseline phase, study patients had to have an average daily diary pain score of at least (within the last days) on a numeric rating scale ranging from ("no pain") to ... medication up to and including Day1 If fewer than scores are available then baseline consists of all scores that are available Page of (page number not for citation purposes) Health and Quality ... Quality Scale were obtained from all available study patients across all treatment groups Test re-test reliability of the Sleep Quality scores were evaluated using pre-treatment data Intraclass...
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báo cáo hóa học: " Boosting with intranasal dendrimeric Aβ1–15 but not Aβ1–15 peptide leads to an effective immune response following a single injection of Aβ1–40/42 in APP-tg mice" ppt
Ngày tải lên :
19/06/2014, 22:20
... has been demonstrated that non-B cell mechanisms may also have a role in clearing cerebral A [8] A multi-center A vaccine human clinical trial (AN1792) was initiated but was suspended when approximately ... each treatment group Statistical significance was determined using Kruskal-Wallis nonparametric one-way ANOVA (B) Immunostaining with the anti -A rabbit polyclonal antibody, R1282, demonstrates ... brain These data provide further data towards the safety and efficacy of dAβ1–15 immunization as a potential A vaccine, although it is acknowledged that the adverse events observed in the AN1792...
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MODELLING AIDS EPIDEMIC AND TREATMENT WITH DIFFERENCE EQUATIONS K. M. TAMIZHMANI, A. RAMANI, B. pot
Ngày tải lên :
23/06/2014, 00:20
... is accompanied again by an increase The T-cell count after an initial fast decrease stabilizes at levels which are lower than those of healthy individuals but not alarmingly so Finally, a fast ... simulations with finite-precision arithmetic are, in fact, based on generalized cellular automata The advantage of the ultradiscretization procedure is that it is a systematic, algorithmic approach which, ... repulsive and we have appearance of a limit cycle: the populations vary periodically with an asymptotically constant amplitude We have carried out numerical simulations (which are essentially the...
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Problem M - Flat Plate In The X-Y Plane With A Twist doc
Ngày tải lên :
06/07/2014, 09:20
... Materials to display the Define Materials dialog box 18 Highlight the CONC material and click the Modify/Show Material button to display the Material Property Data dialog box 19 In this dialog ... the resultant of all the applied loads lies on a line connecting the support points labeled A and “B”, and thus by simple statics the reaction at support point “C” must be zero Note that you could ... Shells to display the Mesh Selected Shells dialog box 11 Fill in this dialog box as shown in the adjacent figure and click the OK button 12 Select the joints that are labeled A , “B” and “C” in...
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Báo cáo khoa học: " RadioImmunotherapy for adenoid cystic carcinoma: a single-institution series of combined treatment with cetuximab" docx
Ngày tải lên :
09/08/2014, 09:20
... pts Table patient characteristics patient characteristics median age [40 - 77] Epipharynx pts Fossa pterygopalatina pts Maxilla pt tuba auditiva tumour stage pts base of skull Analysis Treatment ... neoplasms with fast neutron radiotherapy Arch Otolaryngol Head Neck Surg 2003, 129(9):944-8 Mizoe JE, Tsujii H, Kamada T, Matsuoka Y, Tsuji H, Osaka Y, Hasegawa A, Yamamoto N, Ebihara S, Konno A: ... EK, Ang KK: Radiotherapy plus cetuximab for locoregionally advanced head and neck cancer: 5-year survival data from a phase randomised trial, and relation between cetuximab-induced rash and survival...
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Báo cáo khoa học: "A single centre experience with sequential and concomitant chemoradiotherapy in locally advanced stage IV tonsillar cancer" doc
Ngày tải lên :
09/08/2014, 09:20
... KK: Data analysis, interpretation, manuscript preparation and approval; DCO: Data analysis, interpretation, manuscript approval; DW: Data collection, Page of 10 analysis, manuscript approval; ... experienced an isolated local failure, one an isolated regional failure, one local and regional failure, one locoregional failure with distant metastases Among the three patients with isolated local and/or ... Chemotherapy added to locoregional treatment for head and neck squamous-cell carcinoma: three meta-analyses of updated individual data MACH-NC Collaborative Group Meta-Analysis of Chemotherapy on Head...
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Báo cáo khoa học: " Long term outcome of adolescent and adult patients with pineal parenchymal tumors treated with fractionated radiotherapy between 1982 and 2003 - a single institution’s experience" pot
Ngày tải lên :
09/08/2014, 09:20
... cases Brain Pathol 2000, 10(1):49-60, Review Anan M, Ishii K, Nakamura T, Yamashita M, Katayama S, Sainoo M, Nagatomi H, Kobayashi H: Postoperative adjuvant treatment for pineal parenchymal tumour ... craniospinal tract was treated Irradiation of the whole brain or the craniospinal tract was performed with a median dose of 35 Gy (30-40 Gy) The craniospinal axis was also treated in both patients with ... lobe at the time of fractionated radiation treatment Radiotherapy All patients were treated with percutaneous fractionated radiotherapy, none with radiosurgery Single doses varied between 1,8 and...
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Báo cáo y học: "Association of a single nucleotide polymorphism in growth differentiate factor 5 with congenital dysplasia of the hip: a case-control study" ppt
Ngày tải lên :
09/08/2014, 13:22
... T, Takatori Y, Saito S, Fujioka M, Sudo A, Uchida A, Yamamoto S, Ozaki K, Takigawa M, Tanaka T, Nakamura Y, Jiang Q, Ikegawa S: A functional polymorphism in the 5'UTR of GDF5 is associated with ... Yoshida A, Tominaga S, Nagano J, Shimizu A, Wakana S, Gondo Y, Noda T, Shiroishi T, Ikegawa S: A novel dominant-negative mutation in Gdf5 generated by ENU mutagenesis impairs joint formation and causes ... JR, Savarirayan R, White SM, Graham JM Jr, Gale RP, Svarch E, Newman WG, Kleckers AR, Francomano CA, Govindaiah V, Singh L, Morrison S, Thomas JT, Warman ML: The mutational spectrum of brachydactyly...
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báo cáo khoa học: "A homoharringtonine-based induction regimen for the treatment of elderly patients with acute myeloid leukemia: a single center experience from China" pptx
Ngày tải lên :
10/08/2014, 22:20
... Coso D, Bardou VJ, Stoppa AM, Braud AC, Bouabdallah R, Sainty D, Mozziconacci MJ, Lafage M, Damaj G, Blaise D, Gastaut JA, Maraninchi D: The benefit of induction chemotherapy in patients age > or ... years Cancer 2004, 101:325-331 Alymara V, Tzouvara E, Vartholomatos G, Chaidos A, Tsiara S, Bourantas KL: A single- center, retrospective study of management Page of (page number not for citation ... 1.3 HA: homoharringtonine + cytarabine; DA: daunorubicin + cytarabine; IDA: idarubicin + cytarabine CR: complete remission; OR: overall response; ED: early death; OS: overall survival treated with...
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Báo cáo y học: " Severe refractory autoimmune hemolytic anemia with both warm and cold autoantibodies that responded completely to a single cycle of rituximab: a case report" pps
Ngày tải lên :
11/08/2014, 00:23
... tachycardia of 110 beats/min The patient was confused, lethargic and pale His physical examination was remarkable for scleral icterus, shifting dullness, hepatosplenomegaly and bilateral lower-extremity ... indicating an anecdotal benefit of rituximab treatment for immune cytopenias, plasmapheresis was discontinued and our patient was placed on rituximab therapy at a dose of 375 mg/m2 every week A ... the basis of the clinical presentation and laboratory analysis, our patient was diagnosed with mixed AIHA Concomitant features of liver dysfunction and nephrotic syndrome secondary to nodular glomerulosclerosis...
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báo cáo khoa học:" Reliability and validity of a single item measure of quality of life scale for adult patients with cystic fibrosis" pot
Ngày tải lên :
11/08/2014, 23:22
... scale measure and body image may suggest that adult patients with CF may have adopted a level of negative image (stigma) of the disease in manner that is different from an adaptation to physical ... patients with a ‘health status worsening’ in order to provide and target appropriate intervention Conclusions The single- item quality of scale is acceptable, valid and repeatable for adult patients ... to the statistical analyses and editing of the manuscripts KW participated in the study design, preparation and the editing of the article All authors have read and approved the final manuscript...
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