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morpholino injection in xenopus

An experimental investigation on hydrogen fuel injection in intake port and manifold with different EGR rates

An experimental investigation on hydrogen fuel injection in intake port and manifold with different EGR rates

Môi trường

... by times in port injection and times in manifold injection however there is an increase in smoke by 36 % in port injection and 44 % in manifold injection The CO emission is found to be increases ... performance of dual injection hydrogen fueled engine by using in- cylinder injection and external fuel injection techniques An increase in thermal efficiency by about 22 % was noted for dual injection at ... for the injector opening Based on the preset timing and duration the injector was opened for injection and closed after injection The injection timing and injection duration was varied within the...
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Tài liệu Báo cáo khoa học: Mosquito (Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes docx

Tài liệu Báo cáo khoa học: Mosquito (Aedes aegypti ) aquaporin, present in tracheolar cells, transports water, not glycerol, and forms orthogonal arrays in Xenopus oocyte membranes docx

Báo cáo khoa học

... AeaAQP is simultaneously expressed in these serotonin receptorexpressing tracheolar cells and if serotonin is in any way involved in modulating water transport in tracheolar cells Hormonal regulation ... (1999) Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice J Clin Invest 103, 555–561 14 Nielsen, S., King, L.S., Christensen, B.M & Agre, P (1997) Aquaporins in complex tissues II Subcellular ... contain BglII restriction sites (bold) used to clone into the pXG-ev1 vector Functional analysis in Xenopus oocytes AQPcic, GlpF and AeaAQP cRNA were prepared in vitro using the mCap mRNA capping...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot

Báo cáo khoa học

... expected initiation methionine by translating the fusion protein GST-XN73 and His6-XN73 As can be seen in Fig 2B, these fusion proteins run at the expected height, proving that no internal methionine ... cell cycle was initially suggested through experiments using okadaic acid (OA), an inhibitor of PP1 and PP2A Induction of meiotic oocyte maturation occurred after injection of OA into Xenopus [16] ... expression is obtained as a consequence of inserting the cDNAs between the 5¢- and 3¢-UTR of b-globin, a protein that is highly expressed in Xenopus laevis oocytes GST-fusion proteins of XPR130,...
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Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt

Báo cáo khoa học: Cell type-specific transgene expression of the prion protein in Xenopus intermediate pituitary cells ppt

Báo cáo khoa học

... protein in Xenopus intermediate pituitary cells In the Xenopus intermediate pituitary, melanotrope cells produce vast amounts of POMC Confocal microscopy using an anti-POMC IgG recognizing only intact ... Belgium [56]), and against the protein-folding chaperones calnexin and binding protein BiP by K Geering (University of Lausanne, Switzerland [57]) Monoclonal anti-tubulin IgG E7 has been described ... construct encoding PrP fused amino-terminally to GFP For the preparation of PrP cDNA, a DNA fragment containing the Xenopus PrP ORF (+ 69 to +648 [42]; was amplified by PCR using primers containing EcoRI...
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Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx

Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx

Báo cáo khoa học

... two-step PCR using HindIII site-containing sense primer and BamHI site-containing antisense primer (Table S1, see Supporting information) [49] The HindIII-BamHI DNA fragments were ligated into the ... the Ca2+-independent ABA-activated SnRK2.6 kinase [21] In addition, the SnRK2.6 kinase could phosphorylate T306 in an in vitro kinase assay This evidence suggests that multiple protein kinases ... membrane voltage which, in turn, results in the opening of inward-rectifying K+ channels, allowing the in ux of K+ ions [4] For stomatal closure, increased levels of cytosolic Ca2+ inhibit plasma membrane...
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Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt

Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt

Báo cáo khoa học

... process they join the localization complex Interestingly, Staufen has been found to interact with kinesin in X laevis oocytes, and might therefore provide the link between the localizing particle ... expressing the TAP-tag only (TAP) was included Proteins contained in these preparations were separated by SDS ⁄ PAGE and visualized by colloidal Coomassie staining (Fig 1A); the arrays of proteins interacting ... ribosomes; sensitivity to RNAse treatment indicates that the RNA serves as a scaffold for protein binding rather than protein–protein interactions providing the driving force for the formation of these...
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Modeling CO2 Injection in Fractured Reservoirs Using Single Matrix Block Systems ppt

Modeling CO2 Injection in Fractured Reservoirs Using Single Matrix Block Systems ppt

Tự động hóa

... of injection rate on 0.8 md core during CO gas injection for Haft Kel lab system at 1000 psia 135 Figure 7.14 – Effect of injection rate on 0.8 md single matrix block during CO gas injection ... of injection rate on 0.8 md core during CO gas injection for C -C lab system at 1000 psia 134 xxiv List of Figures Figure 7.12 – Effect of injection rate on md core during CO gas injection ... publication in the Journal of Petroleum Science and Engineering) viii List of Paper Alavian, S.A., and Whitson C.H 2011 Modeling CO Injection Including Diffusion in a Fractured-Chalk Experiment with Initial...
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Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc

Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc

Báo cáo khoa học

... homeodomain motifs It is a 124 kDa DNA-binding protein which was initially characterized as a negative regulatory factor involved in the lens-specific regulation of the avian gene encoding d-crystallin ... microinjection into X laevis embryos To investigate whether either or both XlMGP transcripts are present during gastrulation, a series of reporter constructs were injected radially into the marginal ... difference in increase in luciferase activity is consistent with the other results obtained, namely the intensity of the RT-PCR bands and the weak in situ hibridization signal at stage 12 Injection...
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Báo cáo khoa học: Apoptosis-inhibiting activities of BIR family proteins in Xenopus egg extracts ppt

Báo cáo khoa học: Apoptosis-inhibiting activities of BIR family proteins in Xenopus egg extracts ppt

Báo cáo khoa học

... repeat, in which IAP stands for inhibitor of apoptosis protein) domain is an evolutionarily conserved zinc-binding motif Many, but not all, proteins containing the BIR domain (BIR family proteins) ... for Xenopus survivin2, encodes a similar but distinct molecule of 157 aminoacid residues containing a single BIR domain As a very similar protein, named SIX, was recently identified as an RXRa-interacting ... are distinct proteins rather than the nonallelic isoforms of a single protein in pseudotetraploid X laevis Another cDNA encodes a 401-residue protein containing two N-terminal BIR domains and...
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Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf

Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf

Báo cáo khoa học

... mPER1 contains an additional NLS in its C-terminal domain (A) Mapping of NLS function in murine Per proteins Fragments corresponding to different portions of mPER1, mPER2 and mPER3 (as indicated) ... phosphorimaging Microinjection into Xenopus laevis oocytes Oocytes were prepared for microinjection as described previously [41] All measures were taken to minimise pain and discomfort of the frogs in ... responsible for the masking of the NLS in mPER3 remains to be elucidated The NLS in mPER3 may be masked by intramolecular protein folding or by interaction with an unknown inhibitory factor With...
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FUEL INJECTION IN AUTOMOTIVE ENGINEERING pptx

FUEL INJECTION IN AUTOMOTIVE ENGINEERING pptx

Kĩ thuật Viễn thông

... of ethanol, in the normal fuel injection system for a single cylinder, DI (Direct Injection) oriented engine 8 Fuel Injection in Automotive Engineering Another point to be noted in this case ... the engine at 50% blending of ethanol, in the normal fuel injection system for a single cylinder, DI (Direct Injection) oriented engine Also, point to be noted in this case is that, the engine gives ... the engine gets increased Fig 20 Different layers of deposition based on Alodine EC coating 28 Fuel Injection in Automotive Engineering Fig 21 Engine parts with alodine EC coating Fig 22 Engine...
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Modeling CO2 Injection in Fractured Reservoirs Using Single Matrix Block Systems pptx

Modeling CO2 Injection in Fractured Reservoirs Using Single Matrix Block Systems pptx

Tự động hóa

... of injection rate on 0.8 md core during CO gas injection for Haft Kel lab system at 1000 psia 135 Figure 7.14 – Effect of injection rate on 0.8 md single matrix block during CO gas injection ... of injection rate on 0.8 md core during CO gas injection for C -C lab system at 1000 psia 134 xxiv List of Figures Figure 7.12 – Effect of injection rate on md core during CO gas injection ... publication in the Journal of Petroleum Science and Engineering) viii List of Paper Alavian, S.A., and Whitson C.H 2011 Modeling CO Injection Including Diffusion in a Fractured-Chalk Experiment with Initial...
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Báo cáo khoa học:

Báo cáo khoa học: "Disposition kinetics and urinary excretion of cefpirome after intravenous injection in buffalo calves" doc

Báo cáo khoa học

... pihsralohcS tireM ytisrevinU a fo mrof eht ni ytisrevinU secneicS laminA dna yranireteV veD dagnA uruG morf deviecer ecnatsissa laicnanif eht segdelwonkca ylud rohtua roines ehT stnemgdelwonkcA ... noisnepsus a dna ,h 42 rof Co73 ta oN muidem no derutluc erew smsinagro tset ehT msinagro tset eht sa )937 CCTM( gnisu ]2[ euqinhcet yassaoib lacigoloiborcim dradnats a yb denimreted erew selpmas ... rednu aera ;CUA ,noitartnecnoc yrotibihni muminim ;CIM :etontooF ss ,B aera snegohtap lamina nommoc tsniaga emoripfec fo noitartnecnoc yrotibihni muminim eht si ∝)nim(pC dna ,lavretni egasod eht...
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Báo cáo y học:

Báo cáo y học: "Therapeutic efficacy of intra-articular adrenomedullin injection in antigen-induced arthritis in rabbits" ppt

Báo cáo khoa học

... adrenomedullin following arthritis adrenomedullin injection in rabbits with antigen-induced articular Sequential concentrations plasma adrenomedullin following intraintra-articular adrenomedullin injection ... treated with daily injections of adrenomedullin (AM) or saline (control) into the knee joint spaces beginning 24 hours after arthritis onset Joint swelling was defined as the increase in knee diameter ... treated with saline (a) Joint swelling progress in rabbits with AIA treated with daily intra-articular injections of AM or saline for days (n = in each group) Daily intra-articular injections of...
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Báo cáo y học:

Báo cáo y học: "Early response predicts subsequent response to olanzapine long-acting injection in a randomized, double-blind clinical trial of treatment for schizophrenia" potx

Báo cáo khoa học

... baseline-to-Week 12 improvement across all functional subdomains of the Schizophrenia Objective Functioning Instrument, a validated functional measure including subdomains of living situation, instrumental ... scale that consists of a semi-structured interview administered by a trained clinician and provides information on symptoms and functioning during the preceding weeks Each item is scored Page of ... treatment In this instance, the later time point was chosen because for many of the patients in this study, weeks represented the interval between injections, and was therefore the earliest point at...
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Báo cáo y học:

Báo cáo y học: "Dose-associated changes in safety and efficacy parameters observed in a 24-week maintenance trial of olanzapine long-acting injection in patients with schizophrenia" pdf

Báo cáo khoa học

... prolactin, both of which exhibited mean increases with increasing dose The resulting scatterplots, trendlines, and regression equations are shown in Figure for weight and in Figure for prolactin ... (HDL) cholesterol normal at baseline to low at endpoint and fasting triglycerides normal at baseline to high at endpoint, the incidence of which increased with increasing dose Efficacy and Effectiveness ... 003), indicating declining relapse rates with increasing dose A statistically significant dose association based on regression analysis was identified for baseline-to-endpoint mean change in PANSS...
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báo cáo khoa học:

báo cáo khoa học: " Assisted injection in outdoor venues: an observational study of risks and implications for service delivery and harm reduction programming" pps

Báo cáo khoa học

... living on the street, in a shelter or hostel Drug use variables of interest included: years injecting (per year), police presence (yes/no), daily heroin injection (yes/no), daily cocaine injection ... ability of IDU to administer their own injections Such infections have been also associated with frequent cocaine injection [20,21] In addition, daily cocaine injection remains a strong predictor ... engage in this practice in outdoor venues In the present study, we demonstrated that being female was associated with receiving assistance with injecting in outdoor settings This finding is consistent...
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Báo cáo y học:

Báo cáo y học: " Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression" pptx

Báo cáo khoa học

... transcripts identified in this work was unaffected in morpholino -cateninventralized embryos Similar results were obtained by macroarray analysis, indicating that novel signaling pathways contribute ... plays an essential role in establishing dorso-ventral patterning in Xenopus embryos [1] Activation of this signaling pathway in the whole embryo produces dorsalization and its inhibition generates ... expression in the dorso-ventral axis Interestingly, the expression of three transcripts was independent of -catenin signaling To the best of our knowledge, this is the first SAGE experiment in Xenopus...
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IDENTIFICATION OF PUTATIVE TARGETS OF NKX2-5 IN XENOPUS LAEVIS USING CROSS-SPECIES ANNOTATION AND MICROARRAY GENE EXPRESSION ANALYSIS

IDENTIFICATION OF PUTATIVE TARGETS OF NKX2-5 IN XENOPUS LAEVIS USING CROSS-SPECIES ANNOTATION AND MICROARRAY GENE EXPRESSION ANALYSIS

Y khoa - Dược

... microinjection of synthetic mRNA Directly injecting mRNA into the developing embryo allows a researcher to perturb a developmental gene network by overexpressing a gene, introducing a dominant-negative, ... et al 1999) The binding sites of murine Nkx2-5 has been characterized by Chen and Schwartz and been shown to have two distinct binding sites: an NK2 domain and a homeodomain binding site(Chen et ... from the Xenopus laevis "-globin gene which aids with mRNA stability and translation in vivo Inserts into this plasmid can be easily transcribed into RNA in vitro using a T7 RNA polymerase In microarray...
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