... by times in port injection and times in manifold injection however there is an increase in smoke by 36 % in port injection and 44 % in manifold injection The CO emission is found to be increases ... performance of dual injection hydrogen fueled engine by using in- cylinder injection and external fuel injection techniques An increase in thermal efficiency by about 22 % was noted for dual injection at ... for the injector opening Based on the preset timing and duration the injector was opened for injection and closed after injection The injection timing and injection duration was varied within the...
... AeaAQP is simultaneously expressed in these serotonin receptorexpressing tracheolar cells and if serotonin is in any way involved in modulating water transport in tracheolar cells Hormonal regulation ... (1999) Lung fluid transport in aquaporin-1 and aquaporin-4 knockout mice J Clin Invest 103, 555–561 14 Nielsen, S., King, L.S., Christensen, B.M & Agre, P (1997) Aquaporins in complex tissues II Subcellular ... contain BglII restriction sites (bold) used to clone into the pXG-ev1 vector Functional analysis inXenopus oocytes AQPcic, GlpF and AeaAQP cRNA were prepared in vitro using the mCap mRNA capping...
... expected initiation methionine by translating the fusion protein GST-XN73 and His6-XN73 As can be seen in Fig 2B, these fusion proteins run at the expected height, proving that no internal methionine ... cell cycle was initially suggested through experiments using okadaic acid (OA), an inhibitor of PP1 and PP2A Induction of meiotic oocyte maturation occurred after injection of OA into Xenopus [16] ... expression is obtained as a consequence of inserting the cDNAs between the 5¢- and 3¢-UTR of b-globin, a protein that is highly expressed inXenopus laevis oocytes GST-fusion proteins of XPR130,...
... protein inXenopus intermediate pituitary cells In the Xenopus intermediate pituitary, melanotrope cells produce vast amounts of POMC Confocal microscopy using an anti-POMC IgG recognizing only intact ... Belgium [56]), and against the protein-folding chaperones calnexin and binding protein BiP by K Geering (University of Lausanne, Switzerland [57]) Monoclonal anti-tubulin IgG E7 has been described ... construct encoding PrP fused amino-terminally to GFP For the preparation of PrP cDNA, a DNA fragment containing the Xenopus PrP ORF (+ 69 to +648 [42]; was amplified by PCR using primers containing EcoRI...
... two-step PCR using HindIII site-containing sense primer and BamHI site-containing antisense primer (Table S1, see Supporting information) [49] The HindIII-BamHI DNA fragments were ligated into the ... the Ca2+-independent ABA-activated SnRK2.6 kinase [21] In addition, the SnRK2.6 kinase could phosphorylate T306 in an in vitro kinase assay This evidence suggests that multiple protein kinases ... membrane voltage which, in turn, results in the opening of inward-rectifying K+ channels, allowing the in ux of K+ ions [4] For stomatal closure, increased levels of cytosolic Ca2+ inhibit plasma membrane...
... process they join the localization complex Interestingly, Staufen has been found to interact with kinesin in X laevis oocytes, and might therefore provide the link between the localizing particle ... expressing the TAP-tag only (TAP) was included Proteins contained in these preparations were separated by SDS ⁄ PAGE and visualized by colloidal Coomassie staining (Fig 1A); the arrays of proteins interacting ... ribosomes; sensitivity to RNAse treatment indicates that the RNA serves as a scaffold for protein binding rather than protein–protein interactions providing the driving force for the formation of these...
... of injection rate on 0.8 md core during CO gas injection for Haft Kel lab system at 1000 psia 135 Figure 7.14 – Effect of injection rate on 0.8 md single matrix block during CO gas injection ... of injection rate on 0.8 md core during CO gas injection for C -C lab system at 1000 psia 134 xxiv List of Figures Figure 7.12 – Effect of injection rate on md core during CO gas injection ... publication in the Journal of Petroleum Science and Engineering) viii List of Paper Alavian, S.A., and Whitson C.H 2011 Modeling CO Injection Including Diffusion in a Fractured-Chalk Experiment with Initial...
... homeodomain motifs It is a 124 kDa DNA-binding protein which was initially characterized as a negative regulatory factor involved in the lens-specific regulation of the avian gene encoding d-crystallin ... microinjection into X laevis embryos To investigate whether either or both XlMGP transcripts are present during gastrulation, a series of reporter constructs were injected radially into the marginal ... difference in increase in luciferase activity is consistent with the other results obtained, namely the intensity of the RT-PCR bands and the weak in situ hibridization signal at stage 12 Injection...
... repeat, in which IAP stands for inhibitor of apoptosis protein) domain is an evolutionarily conserved zinc-binding motif Many, but not all, proteins containing the BIR domain (BIR family proteins) ... for Xenopus survivin2, encodes a similar but distinct molecule of 157 aminoacid residues containing a single BIR domain As a very similar protein, named SIX, was recently identified as an RXRa-interacting ... are distinct proteins rather than the nonallelic isoforms of a single protein in pseudotetraploid X laevis Another cDNA encodes a 401-residue protein containing two N-terminal BIR domains and...
... mPER1 contains an additional NLS in its C-terminal domain (A) Mapping of NLS function in murine Per proteins Fragments corresponding to different portions of mPER1, mPER2 and mPER3 (as indicated) ... phosphorimaging Microinjection into Xenopus laevis oocytes Oocytes were prepared for microinjection as described previously [41] All measures were taken to minimise pain and discomfort of the frogs in ... responsible for the masking of the NLS in mPER3 remains to be elucidated The NLS in mPER3 may be masked by intramolecular protein folding or by interaction with an unknown inhibitory factor With...
... of ethanol, in the normal fuel injection system for a single cylinder, DI (Direct Injection) oriented engine 8 Fuel Injectionin Automotive Engineering Another point to be noted in this case ... the engine at 50% blending of ethanol, in the normal fuel injection system for a single cylinder, DI (Direct Injection) oriented engine Also, point to be noted in this case is that, the engine gives ... the engine gets increased Fig 20 Different layers of deposition based on Alodine EC coating 28 Fuel Injectionin Automotive Engineering Fig 21 Engine parts with alodine EC coating Fig 22 Engine...
... of injection rate on 0.8 md core during CO gas injection for Haft Kel lab system at 1000 psia 135 Figure 7.14 – Effect of injection rate on 0.8 md single matrix block during CO gas injection ... of injection rate on 0.8 md core during CO gas injection for C -C lab system at 1000 psia 134 xxiv List of Figures Figure 7.12 – Effect of injection rate on md core during CO gas injection ... publication in the Journal of Petroleum Science and Engineering) viii List of Paper Alavian, S.A., and Whitson C.H 2011 Modeling CO Injection Including Diffusion in a Fractured-Chalk Experiment with Initial...
... adrenomedullin following arthritis adrenomedullin injectionin rabbits with antigen-induced articular Sequential concentrations plasma adrenomedullin following intraintra-articular adrenomedullin injection ... treated with daily injections of adrenomedullin (AM) or saline (control) into the knee joint spaces beginning 24 hours after arthritis onset Joint swelling was defined as the increase in knee diameter ... treated with saline (a) Joint swelling progress in rabbits with AIA treated with daily intra-articular injections of AM or saline for days (n = in each group) Daily intra-articular injections of...
... baseline-to-Week 12 improvement across all functional subdomains of the Schizophrenia Objective Functioning Instrument, a validated functional measure including subdomains of living situation, instrumental ... scale that consists of a semi-structured interview administered by a trained clinician and provides information on symptoms and functioning during the preceding weeks Each item is scored Page of ... treatment In this instance, the later time point was chosen because for many of the patients in this study, weeks represented the interval between injections, and was therefore the earliest point at...
... prolactin, both of which exhibited mean increases with increasing dose The resulting scatterplots, trendlines, and regression equations are shown in Figure for weight and in Figure for prolactin ... (HDL) cholesterol normal at baseline to low at endpoint and fasting triglycerides normal at baseline to high at endpoint, the incidence of which increased with increasing dose Efficacy and Effectiveness ... 003), indicating declining relapse rates with increasing dose A statistically significant dose association based on regression analysis was identified for baseline-to-endpoint mean change in PANSS...
... living on the street, in a shelter or hostel Drug use variables of interest included: years injecting (per year), police presence (yes/no), daily heroin injection (yes/no), daily cocaine injection ... ability of IDU to administer their own injections Such infections have been also associated with frequent cocaine injection [20,21] In addition, daily cocaine injection remains a strong predictor ... engage in this practice in outdoor venues In the present study, we demonstrated that being female was associated with receiving assistance with injecting in outdoor settings This finding is consistent...
... transcripts identified in this work was unaffected inmorpholino -cateninventralized embryos Similar results were obtained by macroarray analysis, indicating that novel signaling pathways contribute ... plays an essential role in establishing dorso-ventral patterning inXenopus embryos [1] Activation of this signaling pathway in the whole embryo produces dorsalization and its inhibition generates ... expression in the dorso-ventral axis Interestingly, the expression of three transcripts was independent of -catenin signaling To the best of our knowledge, this is the first SAGE experiment in Xenopus...
... microinjection of synthetic mRNA Directly injecting mRNA into the developing embryo allows a researcher to perturb a developmental gene network by overexpressing a gene, introducing a dominant-negative, ... et al 1999) The binding sites of murine Nkx2-5 has been characterized by Chen and Schwartz and been shown to have two distinct binding sites: an NK2 domain and a homeodomain binding site(Chen et ... from the Xenopus laevis "-globin gene which aids with mRNA stability and translation in vivo Inserts into this plasmid can be easily transcribed into RNA in vitro using a T7 RNA polymerase In microarray...