molecular aspects of immunoglobulin expression by human b cell leukemias and lymphomas

Báo cáo khoa học: Control of transferrin expression by b-amyloid through the CP2 transcription factor pdf

Ngày tải lên : 23/03/2014, 03:20

... IA, Weksler B, Stanimirovic DB & Zhang W (2009) Expression of inﬂammatory genes induced by beta-amyloid peptides in human brain endothelial cells and in Alzheimer’s brain is mediated by the JNK-AP1 ... compared to the control The level of CP2 expression in each group of cells was conﬁrmed by Western blotting, an example of which is shown (bottom panel) Expression of b- tubulin was used as a loading ... amyloid b- protein (Ab) in human neuroblastoma cells Biochemistry 39, 6951–6959 18 Blass JP, Baker AC, Ko L & Black RS (1990) Induction of Alzheimer antigens by an uncoupler of oxidative phosphorylation...

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Báo cáo y học: " Augmentation of Pulmonary Epithelial Cell IL-8 Expression and Permeability by Pre-B-cell Colony Enhancing Factor" docx

Ngày tải lên : 11/08/2014, 08:22

... A549 cell permeability is significantly attenuated by the knockdown of PBEF expression by PBEF siRNA Overexpression of PBEF increased A549 cell permeability in both basal and TNFα Page 12 of 15 ... Figure A549 of PBEF expression on in vitro cell permeability of Effectscells and HPAEC Effects of PBEF expression on in vitro cell permeability of A549 cells and HPAEC A549 cells or HPAEC cells were ... described in the Methods A&C Effects of PBEF underexpression on in vitro cell permeability of A549 cells and HPAEC; B& D Effects of PBEF overexpression on in vitro cell permeability of A549 cells and...

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Retrovirology Research BioMed Central Open Access Induction of p21CIP1/WAF1 expression by human potx

Ngày tải lên : 12/08/2014, 23:20

... described in METHODS The beads were collected by centrifugation and washed with the binding buffer The bound proteins were eluted in SDS sample buffer and analyzed by immunoblotting (Sp1) and Coomassie ... activation of APC/C and inactivation of SCFSkp2 by Tax By contrast, even though the Tax-induced increase in p21 and p27 correlated with APC/C activation, the up-regulation of p21 by Tax is achieved by ... in Fig 5B, the stability of p21 is not significantly altered by Tax (Fig 5B) These results confirm the notion that p27 is a substrate of SCFSkp2 and becomes stabilized as a consequence of premature...

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Tài liệu Báo cáo khoa học: Novel aspects of heat shock factors: DNA recognition, chromatin modulation and gene expression ppt

Ngày tải lên : 18/02/2014, 04:20

... transcription-competent state, thereby enabling robust activation by HSF1 if cells in the early G1 phase are exposed to a stressor [66] The bookmarking function of HSF2 is achieved by recruitment of protein phosphatase ... the binding of HSF, general transcription factors and Pol II to the promoters, and subsequent RNA synthesis by polymerase In mammalian cells, HSF1 target promoters are also bound by HSF2 and ... phosphatase 2A to inhibit nearby condesin complexes by dephosphorylation The bookmarking of the HSP70 promoter and other HSE-containing promoters by HSF2 is different from that of many active promoters,...

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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

Ngày tải lên : 18/02/2014, 18:20

... The expression of Mig ⁄ CXCL9 by cultured FLS is stimulated by IFN-c [39] Stromal cell- derived factor (SDF)-1 ⁄ CXCL12, a ligand of CXCR4, is expressed in the RA synovium and is increased by CD40 ... number variations of CCL3-like (CCL3L1), a nonallelic isoform of CCL3 encoded by different genes and a potent ligand for CCR1 and CCR5, inﬂuenced susceptibility to RA, and genetic interaction between ... Increased expression of MIP-3a ⁄ CCL20 and CCR6 has also been conﬁrmed in tissue biopsies from RA subchondral bone [27] Our group recently found that the mRNA expression of MCP-4 ⁄ CCL13 (a ligand of...

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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of environmental factors pdf

Ngày tải lên : 18/02/2014, 18:20

... amplification of both NS1 and VP genes of B1 9 DNA in synovial membranes has revealed that the levels of B1 9 DNA are similar in RA and control synovial membranes [11] Thus, the contribution of B1 9 infection ... investigators observed that incubation with B1 9-containing serum induces an invasive phenotype in normal human synovial fibroblasts and that B1 9 serum-treated synovial fibroblasts exhibit an increase ... invasive phenotype by human parvovirus B1 9 in normal human synovial fibroblasts Arthritis Rheum 44, 1582–1586 Lu J, Zhi N, Wong S & Brown KE (2006) Activation of synoviocytes by the secreted phospholipase...

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Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: role of transcription factors ppt

Ngày tải lên : 18/02/2014, 18:20

... alteration of the NF-jB p52 ⁄ p100 subunit encoded by the NFKB2 gene has been reported in certain T -cell lymphomas, chronic lymphocytic leukemias, myelomas and B- cell lymphomas Ampliﬁcation of the ... of T cells has been shown to have roles in the bone destruction of RA Bone destruction has been shown to be caused by an abnormal activation of the immune system in RA Osteoclasts are cells of ... time-dependent and dosedependent expression of intercellular adhesion molecule-1 and vascular cell adhesion molecule as IL- 1b and TNF-a, and that their expression was partially mediated by NF-jB signaling...

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Báo cáo khoa học: Structural and biological effects of a b2- or b3-amino acid insertion in a peptide Application to molecular recognition of substance P by the neurokinin-1 receptor ppt

Ngày tải lên : 08/03/2014, 08:20

... conformers of Ac -b2 -HAlaNHMe obtained with DISCOVER and AMBER forcefield Table S3 Minimum-energy conformers of Ac -b3 -HAlaNHMe obtained with DISCOVER and AMBER forcefield Table S4 NMR Parameters of SP and ... the substitution of the flexible Gly by the even more flexible HGly causes a 30- to 40-fold decrease in affinity and biological activity Substitution by b3 -HAla has similar effects Only the b2 -HAla ... screening of 4Ær and no cut-off was used Minimum-energy conformers of b- and a-amino acids were generated by molecular dynamics at high temperature followed by energy minimization Two thousand structures...

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Báo cáo khoa học: Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosaminidase pot

Ngày tải lên : 14/03/2014, 23:20

... structure of human b- hexosaminidase B: understanding the molecular basis of Sandhoff and Tay–Sachs disease J Mol Biol 327, 1093–1109 Brumer H, Sims PFG & Sinnott ML (1999) Lignocellulose degradation by ... stronger ﬂuctuation of residues at pH (Fig S2) The lower stability of the protein at pH could be explained by the protonation of Glu, Asp and His residues and by the loss of some stabilizing interactions ... and standard (magenta) substrates after ns of MD (C) Overlay of positions of N-propionyl modiﬁed (light blue) and standard (magenta) substrates after ns of MD (D) Molecular surface of the binding...

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Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot

Ngày tải lên : 16/03/2014, 12:20

... [22] To get a better understanding of the importance of Asn130 in substrate binding and recognition by arginase I, this residue was changed to aspartate by sitedirected mutagenesis, and the kinetic ... determined by staining with Coomassie Brilliant Blue R250 and western blotting using an antibody to human liver arginase Site-directed mutagenesis The N130D and N130Q mutant forms were obtained by a ... markers is indicated by pencil marks on the membrane The arrow indicates the position of the wild-type and N130D variants of human arginase, as detected by an antibody to human arginase I 0.45...

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Secrets of Closing Sales by Charles B. Roth and Roy Alexander pot

Ngày tải lên : 23/03/2014, 11:20

... whereby Chambers can rent a meeting site at less money by bartering closed-out solar products in exchange for space Says Boue: "This will reduce your cash outlay by $4,200." Just then, Chambers' boss, ... how Boue closed throughout: Boue adds: "You've been reaping the benefits of that Off the cuff? Far from it Dave Boue went in loaded for 10 bear Canned presentation? No Boue stored a number of ... you coffee?" But Boue says: "No, thanks I brought my own." From his briefcase, he takes a small compact coffee maker and a small silver cup He tells Chambers, "I have a special blend made in Brazil...

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Molecular Regulation of Endocytosis Edited by Brian Ceresa pot

Ngày tải lên : 24/03/2014, 02:21

... Endophilin has been detected by immunogold labeling at the rim of shallow coated pits (32) These obervations are possibly compatible with a membrane bending role of endophilin at an early stage of endocytosis, ... comprise of Sla1p, Lsb3p, Pan1p, and End3p The NPF module proteins consist of Las17p, type I myosins Myo3p and Myo5p, and Vrp1p, Bzz1p and Bbc1p With the exception of the type I myosins, it should be ... membrane resulted in digestion of FBPase by proteinase K Thus, a portion of FBPase is sequestered inside Vid vesicles However, these observations not rule out the prospect of low amounts of FBPase...

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Báo cáo Y học: Repression of FasL expression by retinoic acid involves a novel mechanism of inhibition of transactivation function of the nuclear factors of activated T-cells pptx

Ngày tải lên : 24/03/2014, 03:21

... that is mediated by a subset of the retinoid receptors All-trans-RA inhibits the DNA binding activity of NFAT To understand the molecular mechanism of RA-induced inhibition of NFAT activity, ... was reduced by approximately 70% when the cells received cotreatment with all-trans-RA, and was almost completely inhibited by the addition of CsA Ó FEBS 2002 Repression of NFAT by retinoic ... activation-induced Fas ligand mediate apoptosis of T cell hybridomas: Inhibition of Fas Ligand expression by retinoic acid and glucocorticoids J Exp Med 181, 1673–1682 Mangelsdorf, D.J., Thummel, C., Beato, M.,...

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Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx

Ngày tải lên : 31/03/2014, 07:20

... ADH3 substrate repertoire and here we investigate the enzymatic activity of the human form of ADH3 for this compound Recombinant expression and puriﬁcation of wild-type and mutated forms of the ... for Windows; Biosoft) kcat values are based on a molecular mass of 40 kDa for all proteins Standard errors for Km and kcat values were ADH3–Arg115Ser and GSNO reduction ... by human alcohol dehydrogenase (Eur J Biochem 270) 1251 Table Steady state kinetic constants for the coenzymes NAD+, NADH, NADP+ and NADPH at pH 7.5, catalyzed by wild-type and mutant forms of...

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Báo cáo hóa học: " Characteristics of functionalized nanohydroxyapatite and internalization by human epithelial cell" pdf

Ngày tải lên : 20/06/2014, 22:20

... http://www.nanoscalereslett.com/content/6/1/600 Page of Figure Cell viability assay Cell viability assay showing the effect of varying concentrations of nanoparticles on growth inhibition of human lung epithelial (A549) cancer cells cultured ... internalized within the A549 cells after h and were observed in the cytoplasm of most cells (Figure 8b) Figure 8b (A magnified and B magnified) shows an accumulation of luminescent nanoparticles ... illustrating actual existence of amino acid residue Europium ion (Eu3+) could be used as a luminescent probe in the bimolecular system And Ca ions on the HAP surface could be replaced by the other metal...

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The Project Gutenberg EBook of The GoGetter, by Peter B. Kyne ppt

Ngày tải lên : 28/06/2014, 17:21

... sort of back-fire I can think of to put the Wobblies on the run." "Every office and ship and retail yard could be run by a first-sergeant," Skinner complained "I'm thinking of having reveille and ... straddled the neck of industry and throttled it with absurd theories that a man's back must be bent like an ox-bow and his locks snowy white before he can be entrusted with responsibility and a living ... START OF THIS PROJECT GUTENBERG EBOOK THE GO-GETTER *** Produced by John Hagerson, Kevin Handy, and the Online Distributed Proofreading Team The Go-Getter A Story That Tells You How to be One By...

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Báo cáo y học: " Molecular mechanisms of autoimmunity triggered by microbial infection" potx

Ngày tải lên : 09/08/2014, 07:20

... mice and humans include: the expression of TLR3 by murine but not human macrophages (Table 2); the expression of TLR8 by human but not murine myeloid dendritic cells; and the expression of TLR9 by ... October 2005 Vol No Anders et al Table Toll-like receptor expression on human and mouse immune cell subsets Monocyte/ macrophages Plasmacytoid dendritic cells Myeloid dendritic cells B cells T cells ... dendritic cells and control of regulatory T cells In vertebrates, autoreactive T cells are kept under tight control by a number of mechanisms, including the suppressive effect of regulatory T cells...

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Báo cáo y học: "Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1β-induced NF-κB-mediated inflammation and apoptosis" potx

Ngày tải lên : 09/08/2014, 14:22

... RG: Stimulation by human interleukin of cartilage breakdown and production of collagenase and proteoglycanase by human chondrocytes but not by human osteoblasts in vitro Biochim Biophys Acta 1984, ... by immunoblotting with anti-Bcl-2, anti-Bcl-xL and anti-TNF-α receptor-associated factor (anti-TRAF1) antibodies A time-dependent downregulation of the expression of Bcl-2, Bcl-xL and TRAF1 by ... activation of NF- B is the phosphorylation and degradation of I B , the natural blocker of NF- B [53,54] To test whether inhibition of IL-1β-induced NF- B activation occurs through inhibition of I B ...

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Global orchestration of gene expression by the biological clock of cyanobacteria Carl Hirschie Johnson pot

Ngày tải lên : 09/08/2014, 20:20

... suggests a broadly global mechanism for the cyanobacterial clock system But what is the basis of this global regulation? One possibility could be rhythmic control by RNA polymerase sigma subunits, ... expression of KaiA) and kaiBCp (driving expression of a dicistronic mRNA encoding KaiB and KaiC) KaiA promotes the phosphorylation of KaiC and inhibits its dephosphorylation, while KaiB antagonizes ... component is abolished by KaiC overexpression, but a significant non-rhythmic basal level remains These results indicate that KaiC (probably as part of a complex) coordinates genome-wide gene expression; ...

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Báo cáo y học: "Genome-wide assessment of imprinted expression in human cells." potx

Ngày tải lên : 09/08/2014, 22:24

... were found to be biallelic in lymphoblasts and/ or fibroblasts (Table S1 in Additional file 1) and the AE observed for the genes ZNF215 and GABRG3 was found to be heritable in both cell types (data ... Yes IBD, identical -by- descent; NA, not available; NI, not informative Table Allelic expression observed in identical -by- descent siblings Condition Number of loci Concordant AE in independent IBD ... evidence as defined by Morrison et al [19]; FB, fibroblast cell lines; I, imprinted genes with previously observed parent -of- origin-dependent expression bias; LCL, lymphoblast cell lines; M, maternal;...

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