molecular and biochemical characteristics of the intracellular ca2 handling proteins in the heart
Báo cáo Y học: Molecular and biochemical characteristics of a gene encoding an alcohol acyl-transferase involved in the generation of aroma volatile esters during melon ripening pptx
Ngày tải lên :
31/03/2014, 21:21
... analysis Both CM-AAT1 and CM-AAT2 encode proteins of 461 amino acids with a theoretical molecular mass of 51.5 kDa and 51.8 and a pI of and 8.5, respectively, and 87% identity at the amino acid sequence ... transformed following the instructions provided by the manufacturer (Invitrogen) The strain harbouring the correct construction was incubated in selecting liquid medium according to Invitrogen recommendations, ... [23,24] The production of recombinant proteins was then attempted in yeast In that case, the CM-AAT1-transformed yeast in culture evolved, in the absence of any exogenous precursor, a strong aroma of...
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Tài liệu Báo cáo khoa học: The structure and biological characteristics of the Spirochaeta aurantia outer membrane glycolipid LGLB pdf
Ngày tải lên :
19/02/2014, 16:20
... [46] Interestingly, a decrease in the fluidity of Re LPS, instigated by a Zn2+induced increase in acyl chain order, elevated the production of TNF-a from human monocytes The increase in acyl chain ... corresponded to the loss of the nonreducing end C-G-A unit and ammonium from the molecular ion Further fragmentation gave the fragment ion at m/z 1436.42, owing to the loss of the branching xylose ... Fragments of HSQC and HMBC spectra of compound Labels illustrate assignment of the amide linkage between the amino group of Asp and the carboxyl group of GalA residue E Scheme The structures of oligosaccharides...
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Báo cáo khoa học: An a-proteobacterial type malate dehydrogenase may complement LDH function in Plasmodium falciparum Cloning and biochemical characterization of the enzyme potx
Ngày tải lên :
16/03/2014, 18:20
... containing the enzyme and the inhibitors were preincubated for 10 before initiating the reaction by addition of NADH Bovine heart LDH (j/solid line); Pf LDH (d/broken line); Pf MDH (h/solid line); ... due to the insertion of five amino acids [14,15] The N-terminal glycine motif GXGXXG is similar to the cofactor binding motif found in most of the LDHs and a-proteobacterial MDHs [12,13,48] In addition, ... the parasite The broken lines/arrows show inhibition and the double lines/ arrows indicate induction of the enzyme/enzymatic reaction in the presence of gossypol the LDH/MDH inhibitor The scheme...
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Báo cáo khoa học: Identification and biochemical characterization of the Anopheles gambiae 3-hydroxykynurenine transaminase pot
Ngày tải lên :
16/03/2014, 23:20
... highlighted the essential role of XA in the gametogenesis and fertility of the malaria parasite [23,24] Very recently, the molecular details of the signalling cascade triggered by XA and resulting in the ... obtained by adding increasing concentrations of the molecule to the reaction samples containing mm d,l-3HK as the amino donor The amount of XA produced after a 5-min incubation was measured and ... other amino acids present in the blood meal The effect of pH and temperature on Ag-HKT catalysed synthesis of XA We examined next the pH optimum and temperature dependence of the 3-HK transamination...
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Báo cáo sinh học: "Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" pot
Ngày tải lên :
18/06/2014, 22:20
... results and drafted the manuscript GT participated in the design and coordination of the study and in the discussion of the results RSA conceived the study, and participated in its design and coordination ... molecules was analyzed in the presence and in the absence of CIITA In the absence of CIITA, Tax-2 localizes both and in the cytoplasm and in the nucleus of 293T cells often with a punctuated ... of Tax-2 and CIITA both in the cytoplasm and the nucleus, and the role of CIITA in redirecting, upon binding, Tax-2 molecules mostly in the cytoplasm These results are discussed within the present...
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o cáo hóa học:" Molecular and cellular correlates of the CIITA-mediated inhibition of HTLV-2 Tax-2 transactivator function resulting in loss of viral replication" potx
Ngày tải lên :
20/06/2014, 04:20
... results and drafted the manuscript GT participated in the design and coordination of the study and in the discussion of the results RSA conceived the study, and participated in its design and coordination ... molecules was analyzed in the presence and in the absence of CIITA In the absence of CIITA, Tax-2 localizes both and in the cytoplasm and in the nucleus of 293T cells often with a punctuated ... of Tax-2 and CIITA both in the cytoplasm and the nucleus, and the role of CIITA in redirecting, upon binding, Tax-2 molecules mostly in the cytoplasm These results are discussed within the present...
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Báo cáo lâm nghiệp:"Water relations and hydraulic characteristics of three woody species co-occurring in the same habitat" pptx
Ngày tải lên :
08/08/2014, 01:21
... (see Fig 7) The centre solid line is the linear regression and the curved dotted lines are 95% confidence intervals The coefficients of the straight line are reported together with the regression ... extensive understanding of their short-term and longterm changes The typical dynamic sequence of encroachment of abandoned grazing areas of the Karstic region [see above and 9, 10, 24] is in accordance ... 24] and the typical climate has been defined as a pre-Alpine continental climate Mean rainfall is about 1150 mm with a maximum in autumn and a minimum in winter (between January and March) Winter...
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Báo cáo lâm nghiệp: "Sexual reproduction in Populus I. Some physiological and biochemical events of the progamic phase" pdf
Ngày tải lên :
09/08/2014, 04:20
... concanavalin A-peroxidase reaction allowed the visualization of 15 stigmatic and pollinic glycoproteins according Differences to the glycoprotein type of increases were observed, distinct in the compat- ... to the presence of P nigra and P alba pollen tubes inside stigmatic tissues However, increasing protein bands were detectable, 0-20 h after pollination, only in compatible pollinated stigmas The ... callose Biochemical studies focused on pollinic and stigmatic proteins, known to be involved in male-female interactions (Gaude and Dumas, 1987) After silver nitrate staining, qualitative and quantitative...
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Molecular and cellular functions of the alternatively spliced isoforms of GDNF receptor complex in neuronal differentiation
Ngày tải lên :
09/09/2015, 10:13
... this thesis to those who find inspiration and use in its findings and analyses It has been a truly enjoyable and rewarding experience making the observations, generating the hypotheses and uncovering ... that these receptor isoforms have distinct biochemical and neuritogenic functions This thesis details the discoveries of distinct signaling pathways involved in the activation of specific proteins, ... further shown to be intimately involved in NTN and NGF induced neurite outgrowth Collectively, these findings demonstrated the hitherto unrecognized role of specific ligands and receptor isoforms...
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Growth and luminescence characteristics of indium nitride for optoelectronics applications in the 1 55 micron region
Ngày tải lên :
09/09/2015, 11:06
... applications in mind, we have identified and addressed several important growth challenges involving InN Our findings are discussed in the rest of the thesis in the following order: In Chapter 2, the main ... than the corresponding value of 8.5% for the InGaAsP/InP system, enabling better optical confinement and waveguiding These advantages in terms of effective masses, conduction band offset, and ... mismatches of -10% and -12% respectively with InN, resulting in the generation of many misfit dislocations in the growing InN film with densities in the order of 1010 cm-2 Growing an additional InGaN...
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MOLECULAR AND FUNCTIONAL CHARACTERISATION OF THE ROLE OF HYDROGEN SULPHIDE IN SEXUAL MEDICINE
Ngày tải lên :
12/10/2015, 17:33
... muscles of the helicine arteries are relaxed, blood inflow to the lacunar spaces increases Relaxation of the smooth muscle of the trabeculae then dilates the lacunae, allowing for the expansion of the ... tissue against the tunica albuginea which in the process, compresses the subtunical venules against the tunica (the stretching of the tunica also compresses the emissary veins), restricting the venous ... effects of H2S may be twofold; being involved in 1) the relaxation of the corporal smooth muscle; and 2) the inhibition of the penile basal tone The nature and site of H2S effects (molecular/ cellular/neurovascular)...
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Molecular and morphological phylogenetics of the faviidae (scleractinia) in singapore
Ngày tải lên :
26/11/2015, 12:47
... use of the COI barcode becomes a standard practice among coral scientists The increasing ease and falling cost of DNA sequencing has not only generated interest in the barcoding of the Scleractinia—reconstructions ... zooxanthellate species, i.e those containing symbiotic dinoflagellates, and these form the basis of coral reefs along the tropical and subtropical coasts of the Indo-Pacific and Atlantic Azooxanthellate ... further away from the mainland, likely due to a declining gradient of sedimentation However, no clarification of species limits in these marginal habitats was attempted In the recent update of...
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Báo cáo khoa học: Proteomic and biochemical analysis of 14-3-3-binding proteins during C2-ceramide-induced apoptosis pot
Ngày tải lên :
06/03/2014, 22:21
... apoptosis With the aim of further analyzing the role of 14-3-3 proteins in apoptosis, an evaluation of the ability of proteins to bind and to be regulated by 14-3-3 proteins during C2-ceramide-induced ... staining of proteins in cell extracts suggests that some proteins could be cleaved during apoptosis initiation; this fact suggests an interesting hypothesis concerning the role of 14-3-3-binding ... overlay assay and with antibodies against the indicated proteins The molecular masses of the proteins indicated by western blotting were in agreement with the masses predicted from the gene sequences...
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Báo cáo Y học: A comparative biochemical and structural analysis of the intracellular chorismate mutase (Rv0948c) from Mycobacterium tuberculosis H37Rv and the secreted chorismate mutase (y2828) from Yersinia pestis pptx
Ngày tải lên :
17/03/2014, 17:20
... cleavage by subtilisin between the prodomain and the target protein, thus releasing the target protein in its native form, beginning with the initiator methionine Cloning of Rv0948c and MT0975 genes ... also report on the cloning of the gene encoding the secreted CM from Yersinia pestis (*YpCM, y2828), purification of the protein, investigation of the properties of the enzyme, and the crystal structure ... in stick form, and the rest of the structure is in cartoon form In EcCM, one polypeptide chain is gray and the other is rose In 90-MtCM, one polypeptide chain is blue and the other is green The...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
Ngày tải lên :
18/02/2014, 18:20
... bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then recording the CD spectra of the incubated samples at indicated temperatures Thermophilicity ... the interface between the DNA binding and the active site subdomains, we managed to model the entire domain without it Another significant finding concerns the nature of the acid residues within ... b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure However, because the Zn-stem loop is a fairly self-standing structure protruding from...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
Ngày tải lên :
21/02/2014, 15:20
... the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding ... illustrates the overall saddle shape of the dimeric structure and the key heparin-binding residues in the N-terminal domain held coordinately by the two subunits on the top of the hollow (Fig 1A) The ... hypothesis was suggested when Razzaghi et al demonstrated in a molecular modeling experiment that the C-terminal domain of apoCII interacts with the interface of the N- and C-terminal domains of...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
Ngày tải lên :
07/03/2014, 15:20
... that an insertion of 37 amino acid peptide segment between the ligand binding domain and the 2826 A Pisarchik and A Slominski (Eur J Biochem 271) Table Molecular mass of the CRFR1 proteins expressed ... the ligand binding domain and the first transmembrane domain (Fig 1B) Protein expression To verify that the constructs produce proteins of the expected masses, we attached the V5 epitope to the C ... urocortin The artificial isoform (CRFR1h2) with the insertion of 37 amino acids between ligand binding domain and the first extracellular loop was able to produce 2828 A Pisarchik and A Slominski...
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Báo cáo khoa học: Molecular and functional characterization of a novel splice variant of ANKHD1 that lacks the KH domain and its role in cell survival and apoptosis docx
Ngày tải lên :
07/03/2014, 21:20
... that the interaction between VBARP and Vpr is specific The input panel represents the amount of protein used in this assay indicating that an equal amount of protein was used in all our samples and ... transmembrane domains in VBARP Also, the use of unsynchronized cells in these analyses further confirmed that the cytoplasmic distribution of VBARP is independent of the cycling stage of the cells Identification ... ankyrin repeat proteins, using the consensus established by Kohl et al [10] and Mosavi et al [11], which contains ankyrin repeats from over 4000 proteins, indicated that the ankyrin repeat domains...
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Báo cáo Y học: Purification and biochemical characterization of some of the properties of recombinant human kynureninase pptx
Ngày tải lên :
08/03/2014, 22:20
... by the Lineweaver– Burk plot in Fig corroborates this with its reduced Vmax and increased Km The shape of the plot is consistent with binding of the inhibitor to both the free enzyme (E) and the ... [15] and hence it can be Scheme inferred that an additional ligand-binding site must be present on the human enzyme When the lines intersect above the x-axis then Ki < Ki¢, and when the lines intersect ... intersect below the x-axis then Ki > K 0i (in both instances the lines have to intersect to the left of the y-axis) The data obtained for L-kynurenine gave Ki ¼ 20 lM and Ki¢ ¼ 55 lM, while the D-isomer...
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