modification of pre mrna splicing patterns by antisense oligonucleotides
Tài liệu Báo cáo Y học: Inhibition of nuclear pre-mRNA splicing by antibiotics in vitro pot
Ngày tải lên :
21/02/2014, 15:20
... inhibited by erythromycin, resulting in speci®c inhibition of the second step of pre- mRNA splicing This ®nding, together with recent data on macrolide-mediated inhibition of eukaryotic pre- mRNA transcription ... range 0±500 lM (Fig 2B,C) Conversion of pre- mRNA into excised lariat RNA was reduced by 60% in the presence of 500 lM Cl-tetracycline and by 95% in the presence of 500 lM erythromycin and streptomycin ... splicing of mRNA precursors Nature (London) 313, 552±557 10 Sharp, P.A (1987) Splicing of mRNA precursors Science 235, 766±771 11 von Ahsen, U & Schroeder, R (1991) Streptomycin inhibits splicing of...
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Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
Ngày tải lên :
16/03/2014, 02:20
... Human Ki-1 ⁄ 57 and pre- mRNA splicing G C Bressan et al Effect of SFRS9 on E1A pre- mRNA splicing in the presence of Ki-1 ⁄ 57 Colocalization analysis of Ki-1 ⁄ 57 to nucleoli and splicing speckles ... the functional involvement of Ki-1 ⁄ 57 in regulatory mechanisms of pre- mRNA splicing Although we also observed a significant modification of the E1A mRNA splicing pattern by Ki-1 ⁄ 57(1–222) and ... 2009 FEBS Human Ki-1 ⁄ 57 and pre- mRNA splicing G C Bressan et al A B C D E F Fig Ki-1 ⁄ 57 influences the splicing pattern of the E1A pre- mRNA (A) Diagram showing the splicing events that generate...
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Báo cáo y học: "The ASRG database: identification and survey of Arabidopsis thaliana genes involved in pre-mRNA splicing" doc
Ngày tải lên :
14/08/2014, 14:21
... is that some splicing- related genes might regulate the splicing of other splicing- related genes For example, overexpression of AtGRP7 and atSRp30 is known to affect the splicing of AtGRP8 and ... fraction of genes with any type of alternative splicing, respectively ping is an important post-transcriptional method for controlling the expression of splicing factor coding genes but not the splicing ... genome-wide studies of pre- mRNA splicing in plants ASRG: a database of Arabidopsis splicing- related genes Our up-to-date web-accessible database comprising the Arabidopsis splicing- related genes...
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Báo cáo y học: "Intronic motif pairs cooperate across exons to promote pre-mRNA splicing" docx
Ngày tải lên :
09/08/2014, 20:22
... Mechanisms of fidelity in pre- mRNA splicing Curr Opin Cell Biol 2000, 12:340-345 Carothers AM, Urlaub G, Grunberger D, Chasin LA: Splicing mutants and their second-site suppressors at the dihydrofolate ... combination of regions also co-occurred in another combination of regions Motif pairs found in the UpDp combination all have P-values less than the P-value cutoff of 10-6 by definition; very few of these ... dependence has previously been seen for exonic splicing enhancers [18] and represents a major problem in deciphering the rules governing the regulation of splicing The regional specificities of all individual...
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báo cáo khoa học: " Modification of tobacco plant development by sense and antisense expression of the tomato viroid-induced AGC VIIIa protein kinase PKV suggests involvement in gibberellin signaling" doc
Ngày tải lên :
12/08/2014, 03:21
... transgenic tobacco plants expressing the sense (XS) or antisense (XAS) copy of pkv Growth of Growth of transgenic tobacco plants expressing the sense (XS) or antisense (XAS) copy of pkv (A) Three weekold ... (QPCR) genes of transgenes on the expression of hostanalysis of the effects Real-time Real-time quantitative RT-PCR (QPCR) analysis of the effects of transgenes on the expression of host genes ... silencing of the endogenous tobacco PKV gene by antisense expression resulted in a taller stature and increased numbers of influorescences in transgenic plants The combined results of reversal of stunting...
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Tài liệu Effect modification of air pollution on Urinary 8-Hydroxy-2’-Deoxyguanosine by genotypes: an application of the multiple testing procedure to identify significant SNP interactions ppt
Ngày tải lên :
17/02/2014, 22:20
... variants of the significant genes identified by MTP with significant interactions using the bootstrap method with the combination of coefficients of the main effect and the interaction [6] Page of ... Different findings of effect modification by GSTM1 variation across studies may reflect differences of exposure, outcome and population, measurement errors in exposure or phenotype, and by chance Similar ... inflammation of New Zealand rabbits There are several strengths in this study First, we used MTP model to identify the significance of a group of candidate genes while we examined effect modification by...
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Báo cáo khoa học: Expression and physiological role of CCN4⁄Wnt-induced secreted protein 1 mRNA splicing variants in chondrocytes potx
Ngày tải lên :
07/03/2014, 10:20
... was supported by the fact that overexpression of WISP1v enhanced the gene expression of one of the mineralization markers in chondrocytic cells Moreover, the presence of a novel splicing variant ... for that of gastrointestinal cancers reported by Tanaka et al [17,20] ++, enhanced expression evaluated by real-time RT-PCR; ±, modest expression evaluated by real-time RT-PCR; –, expression ... using overexpression experiments FLAG epitope-tagged WISP1v and WISP1vx were overexpressed by DNA transfection of mammalian expression vectors into HCS-2 ⁄ cells First, overexpression of WISP1v...
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Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
Ngày tải lên :
16/03/2014, 04:20
... GCN5 preferentially participate in control of the apoptotic cell death induced by PMA/ionomycin treatment of the DT40 cell line Up- and downregulation of expression of the E2A or Aiolos genes by ... caspase-6 mRNA level was decreased gradually by PMA/ionomycin treatment by 24 h, and the caspase-10 mRNA level was increased by h and thereafter decreased dramatically by 24 h Expression of caspase-3 ... alternative processing of its pre- mRNA [21] Recently, we revealed not only that HDAC2 upregulates gene expression of EBF1, Pax5, Aiolos, Ikaros and HDAC7, and down-regulates those of E2A, p300/CBP-associated...
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Báo cáo khoa học: Deadenylation of interferon-b mRNA is mediated by both the AU-rich element in the 3¢-untranslated region and an instability sequence in the coding region pot
Ngày tải lên :
17/03/2014, 10:20
... mRNA supplemented by a carrier tRNA) and 10 lL of lipofectine in a total volume of 200 lL of serum free-medium In both cases, the culture media were harvested to measure IFN-b concentration by ... transcripts differed by the length of their poly(A) tail Therefore, total RNA of Hec-1B cells infected by the Sendaı¨ virus was treated (or not) by RNAse H in the presence of oligo(dT) before ... to a marked increase of IFN-b mRNA accumulation at later times of infection resulting from mRNA stabilization and/or absence of transcriptional repression [10,12] Treatment by both actinomycin...
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Báo cáo khoa học: Identification of a novel alternative splicing variant of RGS5 mRNA in human ocular tissues ppt
Ngày tải lên :
23/03/2014, 13:20
... regulation of G protein and G protein-coupled receptors Results Identification of alternative splicing of RGS5 mRNA in human, monkey and rat ocular tissues Identification of up-regulation of RGS5 mRNA ... Alternative splicing of human RGS5 mRNA (RGS5s) Translation start site (ATG) defined as +1 Blocks represent exons and lines represent introns Exon1 Exon RGS mRNA Exon aaaaaa RGS 5-s mRNA Splicing ... Sequence analysis of alternative splicing of RGS5 mRNA (RGS5-s) (A) Blue color (ga) indicates the splicing site Red color ATG is the translation start site (B) Alternative splicing of RGS5 mRNA encodes...
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Báo cáo khoa học: Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome ppt
Ngày tải lên :
30/03/2014, 04:20
... Analysis of complementation of Dnop53 ⁄ GAL::A-NOP53 by truncated mutants of Nop53p (A) Analysis of complementation of strain Dnop53 ⁄ GAL::A-NOP53 by truncated Nop53p mutants, under the control of ... pGAD, expression of N-Nop53p led to lower accumulation of the 7S pre- rRNA intermediate (Fig 5B) The strain expressing C-Nop53p showed higher levels of 7S pre- rRNA and lower levels of the mature ... Fig Truncated mutants of Nop53p still associate with pre- 60S (A) Co-immunoprecipitation of RNA with full-length or truncated Nop53 Northern blot hybridization of RNA co-immunoprecipitated with protein...
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Báo cáo hóa học: " Fabrication of HfO2 patterns by laser interference nanolithography and selective dry etching for III-V CMOS application" pdf
Ngày tải lên :
21/06/2014, 03:20
... Page of beam system (FEI Co.) In order to protect the surface of interest from milling by the Ga + ion beam during sample preparation, a Pt layer was deposited in the FIB on the HfO2/GaAs nanopatterns ... sidewall due to directional chemical etching and the presence of re-deposited reaction by- products on the edges of the HfO2 mesa stripes The values of the r.m.s line roughness (R a ) measured along ... http://www.nanoscalereslett.com/content/6/1/400 Page of Figure AFM images of the HfO2 nanopattern (a) Three-dimensional view of the nanostructured HfO2/GaAs surface morphology (b) Crosssection scan profile of an etched trench...
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Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot
Ngày tải lên :
09/08/2014, 09:22
... and by further 10 of (left) and incubation ation with single doses between h pretreatment with genistein24 hμM hormonal with estradiol 10 μM (right), followed by irradiSurvival of Survival of ... by immediate-plating with estradiol 10 μM (right), followed by irradiaSurvival of PC-3 cells between pretreatment with genistein 10 μM (left) and tion with Survival of PC-3 cells after 24 h pretreatment ... results are explained by differences in the passages of the studied cell lines, previous and present growth conditions, and in the applied methodologies In the interpretation of our data on clonogenic...
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Báo cáo y học: "Genome-wide analysis of mRNA decay patterns during early Drosophila development" pdf
Ngày tải lên :
09/08/2014, 22:23
... http://genomebiology.com/2010/11/9/R93 Page of 27 Figure Diversity of mRNA decay patterns in Drosophila embryos (a) Clusters of mRNA decay profiles in early embryos (E1, E2 and E3 (Figure 1a)) We show a selection of profiles with ... were independently validated by a comprehensive quantitative PCR experiment monitoring the expression of 24 genes chosen to represent the wide spectrum of expression patterns seen in our dataset ... representing a total of 6,342 genes (c) Transcriptome of the early embryo: proportion and gene numbers of non-expressed, purely transcribed and maternally provided mRNAs representing a total of...
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Báo cáo y học: " Genetic modification of Bluetongue virus by uptake of “synthetic” genome segments" potx
Ngày tải lên :
12/08/2014, 01:22
... differentiation; the presence of a PstI site in S7 of BTV8, and the absence of a BglII site in case of S7 of BTV6 (Table 1, and Figure 2, lane and 9) Furthermore, the sequence of this segment S7 ... to be useful to generate mutants of BTV by genetic manipulation of one or more of genome segments [15] This system needs, however, a set of ten complete cDNAs of genome segments to rescue bluetongue ... slopes of the different growth curves were detected, the observed enrichment of this reassortant by passaging (6 times) of a mixture of BTV6/Net08 and BTV6/net08/ S78 could be the result of factors...
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Báo cáo y học: "Efficient inhibition of HIV-1 expression by LNA modified antisense oligonucleotides and DNAzymes targeted to functionally selected binding sites" pdf
Ngày tải lên :
13/08/2014, 09:21
... depends on the nature of the target Blocking expression of HIV-1 in vivo To evaluate the capacity of the antisense LNA to inhibit cellular HIV-1 expression the expression of the viral Gag derived ... %) Figure RNA The effect of antisense LNA on reverse transcription of HIVThe effect of antisense LNA on reverse transcription of HIV1 RNA An equimolar amount (1pmol) of HIV RNA and LNA oligo ... 150 200 Time of reaction (in minutes) Figure The effect of antisense LNA versus RNA and DNA on dimerization of HIV-1 RNA The effect of antisense LNA versus RNA and DNA on dimerization of HIV-1 RNA...
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Báo cáo y học: "The Nordic back pain subpopulation program - individual patterns of low back pain established by means of text messaging: a longitudinal pilot study" pdf
Ngày tải lên :
13/08/2014, 14:20
... course -patterns of LBP in patients treated in a primary care setting when collecting self-reported data once a week Information regarding the number of LBP days the previous week, number of days off ... previously that the identification of distinct subgroups of LBP patients is a necessary step in order to advance our understanding of the causes of LBP and thereby also the indications for treatment ... days off work (question 3) were too infrequent to be suitable for the analysis of course profiles For these reasons, this report deals only with the number of days that subjects were bothered by...
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Báo cáo y học: " Risk assessment of pre-hospital trauma airway management by anaesthesiologists using the predictive Bayesian approach" pptx
Ngày tải lên :
13/08/2014, 23:21
... stay Death Prediction of more than 10 incidences during one year Prediction of 1- 10 incidences during one year 10-50 % probability of an incidence during one year 1-10 % probability of an incidence ... were assigned by the assessor as an expression of the assessor's uncertainty about the occurrence of the top event [22,25,26] These probabilities are not estimates or predictions of some true ... survey of Norwegian helicopter emergency medical service (HEMS) physicians' own perceptions of the safety and quality of pre- hospital airway management in their system [19] iii) Audit of data...
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Báo cáo y học: "Global analysis of alternative splicing regulation by insulin and wingless signaling in Drosophila cells" ppt
Ngày tải lên :
14/08/2014, 21:20
... gene expression values represent the average of log2 ratios for all the probes of a locus The net expression of a splice junction was calculated by subtracting the average log2 expression of all ... [4-8]) The process of alternative pre- mRNA splicing expands the information content of higher eukaryotic genomes by generating multiple mature mRNAs from a single primary transcript, often with functional ... alternative splicing of CD45 pre- mRNA in T cells implicates protein kinase C and Ras Mol Cell Biol 2000, 20:70-80 Konig H, Ponta H, Herrlich P: Coupling of signal transduction to alternative pre- mRNA splicing...
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