mixing in chemical reactors

GraphITA 2011: Selected Papers from the Workshop on Fundamentals and Applications of Graphene pptx

Ngày tải lên : 24/03/2014, 01:20

... particle-hole continuum, resulting in a Fano-like asymmetry of the phonon peak lineshape [12] encoded in a ﬁnite imaginary part χ jv (ωv ) = Using indeed the relation χA† j (ω) = χ jA (ω), and reminding that ... Brillouin zone corners (K and K ) The possibility of engineering graphene band structure by introducing defects, strains or external potentials has gained importance in the recent past, in particular ... respect to the material contained herein Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Preface This volume contains selected papers presented...

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ditorial: hypotheses about protein folding - the proteomic code and wonderfolds" pot

Ngày tải lên : 13/08/2014, 16:20

... boundaries" are physicochemically defined and that protein-folding information is contained in the redundant exon bases Theor Biol Med Model 3:28 Biró JC: Protein folding information in nucleic acids ... conditions of protein folding FEBS Lett 2000, 466:75-79 Pintar A, Pongor S: The "first in- last out" hypothesis on protein folding revisited Proteins 2005, 60:584-590 Biró JC: Indications that "codon ... [GADV]-proteins and origin of life Int J Mol Sci 2009, 10:1525-1537 Rodin SN, Rodin AS: On the origin of the genetic code: signatures of its primordial complementarity in tRNAs and aminoacyl-tRNA...

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Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulﬁde bond formation and their contribution to venom complexity pdf

Ngày tải lên : 18/02/2014, 18:20

... a cysteinyl residue in the PIII disintegrin-like domain, residing in what is called the RGD loop in disintegrin domains (Fig 6; residue 468, VAP1 numbering) The potent integrin-binding inhibitor ... venom Interesting structural features in disintegrin, disintegrin-like and cysteine-rich domains There are several interesting structural features associated with SVMP disintegrin, disintegrin-like ... the protein indicated that it was the processed disintegrin-like and cysteine-rich domains from the PIIIb hemorrhagic toxin jararhagin [36] Since then, several other processed disintegrin-like...

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Tài liệu Báo cáo khoa học: Unfolding of human proinsulin Intermediates and possible role of its C-peptide in folding/unfolding pptx

Ngày tải lên : 19/02/2014, 12:20

... linkages of insulin have been shown to be important in maintaining its native conformation and biological activity [20–25] The double-chain insulin is synthesized in vivo as a single-chain precursor ... (preproinsulin) and folded as proinsulin, in which a connecting peptide of 35 residues links the C terminus of the B chain and N terminus of the A chain After digestion by a speciﬁc set of protein ... protein enzymes in the B-cell granule, proinsulin is converted into insulin and C-peptide of 31 amino acids [26] Previous studies completed on the unfolding process of insulin or proinsulin were often...

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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx

Ngày tải lên : 20/02/2014, 02:21

... corresponding to K364 in MCAD there is an arginine residue, R356 in the SCAD sequence It is striking that this conservative substitution is identical to the clinical mutation in the present case Interestingly, ... MCAD protein detected in the western blots (Fig 1B) Comparison of activity levels of the mutant proteins, as determined by activity staining The apparent inactivity of R256T was further investigated ... procedure proving very effective in securing a pure protein product The results of kinetic analysis are displayed as Michaelis–Menten plots in Fig 3A The Km, Vmax (as determined by the Direct Linear...

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Báo cáo khoa học: Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding pot

Ngày tải lên : 07/03/2014, 00:20

... of incorrect disulfide bonds, especially those within kinetically trapped, structured folding intermediates [13] In recent years, interest in protein folding in vitro has expanded rapidly, mainly ... of intrasubunit disulfide bridges have been assigned to PfPNP by integrating biochemical methodologies with MS [12] It is interesting to note that SsMTAPII and PfPNP contain, in their C-terminal ... oxidative folding mechanism involving the other structural disulfides of the enzyme Although rare in nature, few examples of oxidized CXC have been reported in the literature, including CSC in the Mengo...

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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc

Ngày tải lên : 07/03/2014, 05:20

... folding investigations, stopped-ﬂow mixing instruments are often necessary, which have a mixing dead time of 1–2 ms Experimental analyses of the kinetic and thermodynamic origin of protein thermostability ... unfolding (ku), faster folding (kf), or a combination of the two (Fig 1A) In vitro folding ⁄ unfolding experiments in solution often involve chemical (i.e urea or guanidine hydrochloride, GuHCl) ... and will be discussed in the ﬁnal section Co-chaperonin proteins Co-chaperonin protein 10 (cpn10) works in conjunction with cpn60 to fold substrate proteins in most organisms in nature [19–21] The...

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Báo cáo khoa học: Protein folding includes oligomerization – examples from the endoplasmic reticulum and cytosol doc

Ngày tải lên : 07/03/2014, 06:20

... with associated proteins These subcomplexes can be seen as pre-assembled folding machines, capable of assisting a specific folding step Keeping the machines intact whilst maintaining the freedom to ... chains linked by disulfide bonds The heavy and light chains consist of several domains, each containing one disulfide bond Constant domains are indicated in light blue and variable domains in dark ... bonds in client proteins [82] (Fig 3) PDI has four thioredoxin domains and a C-terminal acidic domain which binds calcium [83] The thioredoxin domains are labeled ‘a’, ‘b’, ‘b¢’ and ‘a¢’ in order...

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Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

Ngày tải lên : 07/03/2014, 10:20

... of IRP1 was visualized in the ﬁrst fractions (Fig 5B, lanes 2–4) The gel in Fig 5A shows many other proteins in each fraction, including those rich in IRP1 To gain insight into the possibility ... analysed on SDS ⁄ PAGE with silver staining [25] or Coomassie Brilliant Blue staining for MS analysis Immunoblotting and protein transfer for amino acid sequencing Proteins were transferred on nitrocellulose ... reference to the recombinant protein (lane 1), little staining in the size region of hIRP1 was observed in the soluble fractions of mitochondria (lanes 2–4) In contrast, protein fractions separated...

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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

Ngày tải lên : 07/03/2014, 15:20

... s/94 °C; min/56 °C; min/72 °C min/94 °C; min/54 °C; min/72 °C min/94 °C; min/57 °C; min/72 °C min/94 °C; min/54 °C; min/72 °C 30 s/94 °C; min/56 °C; min/72 °C 30 s/94 °C; min/57 °C; min/72 °C ... min/56 °C; min/72 °C 30 s/94 °C; min/56 °C; min/72 °C min/94 °C; min/57 °C; min/72 °C in complete medium [SF900II + 10% (v/v) fetal bovine serum] supplemented with 400 lgÆmL)1 Hygromycin B (Invitrogen) ... the GCPII-binding pocket and proposed/ analyzed the amino acid residues involved in the ligand– protein interactions All of the residues identiﬁed are situated within the segment spanning Arg212...

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Báo cáo khoa học: Context-dependent effects of proline residues on the stability and folding pathway of ubiquitin docx

Ngày tải lên : 07/03/2014, 16:20

... determined protein stability, and their involvement in the folding pathway from studies of refolding/unfolding kinetics While the single point mutation P37A is destabilizing by 4.5 kJÆmol)1, in ... to the stabilizing effect of this mutation not being realized in the folding TSE, indicative of the loop between the N-terminal b-hairpin and the main a-helix remaining ﬂexible in the TSE, with ... [56] using a combination of w-value analysis and protein engineering methods to introduce bis-His metal coordination sites to identify native noncovalent interactions involved in the folding TSE...

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Báo cáo Y học: The folding of dimeric cytoplasmic malate dehydrogenase Equilibrium and kinetic studies doc

Ngày tải lên : 08/03/2014, 23:20

... dimer The time points at which the cross-linking was done are as follows: Lane 1, min; lane 2, min; lane 3, min; lane 4, min; lane 5, 10 min; lane 6–15 min; lane 7–25 min; lane 8, 45 min; lane 9, ... understanding the folding and cooperativity of globular protein structure Proteins: Struct Funct Genet 6, 87–103 Ptitsyn, O.B (1994) Kinetic and equilibrium intermediates in protein folding Protein ... that it serves as a universal kinetic intermediate in protein folding and is involved in a number of physiological processes emphasizes its important role in the folding pathway [8] There is now...

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Báo cáo khoa học: Multiple-probe analysis of folding and unfolding pathways of human serum albumin docx

Ngày tải lên : 16/03/2014, 16:20

... Bilirubin binds to HSA at drug binding site I of the domain II [37,38] As shown in Fig 2, bilirubin binding was minimally altered at low concentrations (< M) of GdnHCl but the binding of bilirubin ... Mechanism of folding and unfolding of HSA (Eur J Biochem 271) 1793 Fig GdnHCl-induced unfolding of HSA was probed by the intrinsic tryptophan ﬂuorescence (s) and bilirubin binding (d) Intrinsic tryptophan ... occurred during the folding–unfolding of HSA An intermediate state was identiﬁed in both the unfolding and folding pathways of HSA and we obtained evidence suggesting that the folding of HSA follows...

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Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identiﬁcation of residues essential for glucanosyltransferase activity and folding pot

Ngày tải lên : 16/03/2014, 18:20

... multidomain proteins [28], or for the sequential and independent folding of the single domains – assuming a vectorial mode of folding for Gas1p In any case, the replacement of C74 with a serine could ... segment in the Gas1 protein [13] The glycosylation proﬁle of the proteins was examined Aliquots of medium containing the recombinant proteins were treated with Endo H and analysed by Western blotting ... glycosylated form, indicating that N-linked chains are not required for activity (data not shown) Essential role of C74 in the folding and stability of the GPI-anchored Gas1p To gain insight into the putative...

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Báo cáo khoa học: Abstract P1 – FEBS Datta Plenary Lectureship Award P1-001 Peptide bond formation, cotranslational folding and antibiotics synergism docx

Ngày tải lên : 16/03/2014, 22:20

... tyrosine kinase through its SH2-domains We found that neutrophils lacking Src-family kinases, the ITAM-bearing adapters DAP12 and FcRc or the Syk tyrosine kinase failed to initiate integrin-induced ... nonhistone proteins in a dynamic polymer called chromatin The discoveries that nucleosome remodelling machines and histonemodifying enzymes organize chromatin into accessible (euchromatic) and inaccessible ... bacterial pathogenesis The key determinant of broad spectrum b-lactam resistance in Methicillin superbug strains is the membrane spanning penicillin binding protein 2a (PBP2a), a transpeptidase that...

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Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot

Ngày tải lên : 22/03/2014, 16:21

... intrinsic relationship between the RNA binding and protein folding in RNAUDE Interestingly, treatment of RNAUDE with RNase A did not result in signicant changes of the melting curve, indicating ... component to UDE, potentially indicating that the conformation of RNAUDE may originate in de novo protein folding during translation In summary, the results obtained in complexation experiments ... possible impact of RNA binding on protein structure and substrate-binding ability Proteins that bind and process nucleic acids are often capable of binding to both RNA and singlestranded or double-stranded...

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A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions pptx

Ngày tải lên : 22/03/2014, 18:20

... extend single (I27SÀS)8 polyproteins The constant force caused individual I27 proteins in the chain to unfold, resulting in stepwise increases in length of the molecule following each unfolding event ... combined with polyprotein engineering has become a powerful approach to studying proteins Using this technique, we have investigated the force-dependency of protein folding,[46, 47] unfolding[37, ... conditions, stretching a polyprotein results in a well-defined series of step increases in length, marking the unfolding and extension of the individual modules in the chain.[37] The size of...

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Specification for Casing and Tubing doc

Ngày tải lên : 23/03/2014, 00:20

... by machining or grinding The area affected by grinding or machining shall be blended smoothly into the contour of the coupling The outside diameter of the finished coupling shall be within the ... on the information contained in this document Sound business, scientific, engineering, and safety judgment should be used in employing the information contained herein API is not undertaking to ... nondestructive examination, fully machined coupling blanks, upset casing, electric-welded casing, tubing and pup joints, impact testing, seal ring couplings, test certificates, tensile testing and sulfide...

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Báo cáo khoa học: ThermoFAD, a ThermoﬂuorÒ-adapted ﬂavin ad hoc detection system for protein folding and ligand binding pdf

Ngày tải lên : 23/03/2014, 04:21

... unfolding Binding of the dye to the unfolded protein results in a significant increase in its intrinsic fluorescence (B) Schematic representation of ThermoFAD In this case, the increase in fluorescence ... binding to these regions, masking the signal associated with protein unfolding In both prokaryotic and eukaryotic organisms, genes encoding proteins that contain flavins as prosthetic group are ... most studied protein classes In particular, the fluorescence of the flavin is an intrinsic property that is widely used for biochemical characterisation of flavoproteins By comparing the emission...

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Báo cáo khoa học: The Yin and Yang of protein folding doc

Ngày tải lên : 23/03/2014, 11:20

... any protein, resulting in a currently missing link between the folding and aggregation funnels (Fig 1) Sculpting the energy surface in vivo In the living cell, a large machinery of proteins forms ... formation [46] Interestingly, this was achieved by increasing protein stability and restoring the cooperativity between the two structural domains in the native protein, reducing the number of ... (monitoring the effect of amino acid substitutions on the kinetics of folding and unfolding) have been shown to be unique in their ability to probe the role of individual residues in stabilizing...

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