microscale technologies for tissue engineering ali khademhosseini yanan du bimal rajalingam joseph p vacanti and robert s langer
Biodegradable Polymers as Scaffolds for Tissue Engineering
... Biodegradable Polymers as Scaffolds for Tissue Engineering Stricture of engineered tissue Porous scaffold Tubular tissue Implantation Engineered tissue Bioabsorbable stent Implantation (Stent resorbed) ... tissues is a hard task, because the supply is often disturbed by the tissues produced The ideal route for oxygen and nutrient supply to cells is through capillaries, but sufficient capillary formation ... oxygen and nutrient supply, the pore size is less important than the porosity Another reason, especially for Scaffold type II, is to fill cells as many as possible in the limited space of scaffold...
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CULTURE OF CELLS FOR TISSUE ENGINEERING potx
... osteogenic protein (BMP-7) porcine aortic endothelial cells List of Abbreviations PBSA PECAM PEG PEGDA Pen/strep PEO PET PGA PITC PLA PLGA PNMT RCCS RGD RWV SA SC SDS-PAGE SMC SNAC SR SSEA-3 and ... system is covered by a combination of the key principles, step-by-step protocols for representative established methods, and extensions to other cell types and tissue engineering applications To ... the principles and basic procedures of cell and tissue culture However, it is recognized that people enter a specialized field, such as tissue engineering, from many different disciplines and, for...
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báo cáo hóa học:" Gelatin-layered and multi-sized porous beta-tricalcium phosphate for tissue engineering scaffold" pptx
... hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite-PCL composites Biomaterials 2010, 31:5498-5509 15 Kobayashi S, Yamadi S: ... properties of gelatin itself are not satisfactory for hard tissue applications Hence, the purpose of the present study was to create a 3D scaffold with enhanced mechanical and biological properties ... types of sample were prepared by the above processes and designated with a code for the purpose of this paper [see Additional file 1] Characterization of the β-TCP scaffold The surface morphologies...
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Applying side effects of anti fibrotic compounds to promote neovascularization for tissue engineering
... Phosphoglycerate kinase 6-Phosphofructo-2-kinase/fructose-2,6bisphosphatase-3 (PFKFB3) 6-Phosphofructo-2-kinase/fructose-2,6bisphosphatase-4 (PFKFB4) Plasminogen activator inhibitor Procollagen prolyl-4-hydroxylase ... transcription factor that is upregulated in response to hypoxia Hypoxia is a common physiological stress imposed by low oxygen conditions to cells or tissues HIF-1 is a heterodimer consisting ... cells and myoblasts to engineer vascularized skeletal muscle tissue 12 in vitro Perfusion, vascularization and survival of the muscle tissue constructs after transplantation was improved iv) Co-seeding...
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Applying side effects of anti fibrotic compounds to promote neovascularization for tissue engineering 1
... to assess their potential use in tissue engineering applications vi A sequential co-culture using fibroblasts and endothelial cells was modified and optimized for assessment of in vitro pro-angiogenesis ... Biosynthesis in vitro and in vivo 99 4.8 Applications in Tissue Engineering: Pre-vascularizing PLLA Scaffolds 101 4.9 Advantages of Using PHi in Tissue Engineering 105 CONCLUSIONS 108 REFERENCES ... dimensional scaffolds This demonstrated a proof of concept that PHi could improve neovascularization for tissue engineering applications by pre-vascularization of three dimensional scaffolds viii...
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Development of chitin based materials for tissue engineering applications
... transfer and possibility of foreign body response and (e) provision of a temporary window for incorporation of a micro-vascular system into the tissues As the field of tissue engineering transpires ... THERAPY Organ transplantation SHORTCOMINGS Costly, operative risk, donor shortage and immunosuppression Surgical reconstruction Insufficient graft tissue, inappropriate graft tissue and poor graft function ... dynamic tissue culture environment where tissue- specific mechanical forces such as stretch, pressure and shear forces are applied Gas and nutrient exchange is amplified by a continuous exchange...
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Application of rapid prototyping technology to the fabrication of 3d chitosan scaffold for tissue engineering
... success rate is not as good as in skin 2.1.2 Three-dimensional Bioresorbable Scaffolds in Tissue Engineering The demand for transplant organs and tissues far outpaces the supply, and this gap ... The need to produce tissues and organs for organ transplant due to the acute shortage of tissues and organs [Mooney and Mikos, 1999], and a possible mismatch of tissue types that can result in organ ... manufacturing process of regular shape and irregular scaffolds using the dual dispensing method Chapter Introduction Chapter presents the experimental results and discusses the advantages and improvements...
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Automatic microassembly system for tissue engineering assisted with top view and force control
... sensors, piezoresistive sensors, capacitive sensors and some optical sensors Chapter describes the design and prototype fabrication of the force sensor integrated into the micro probe gripper Chapter ... through scaffold assembly experiments, and a multi-layer scaffold is successfully assembled Finally chapter concludes the work and proposes some research directions for improving the proposed assembly ... assembly speed and short cycle times respectively Good continuous use behavior Suitability for automated assembly Separate gripper control also applicable for sensor processing Table 3-3 Popularly...
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Engineering aggregates with chemical linkers for tissue engineering application
... multicellular structures combining principles from tissue engineering, cell surface engineering and chemistry Instead of using classical tissue engineering approach, which involves seeding cells into polymer ... problems across these spectrums Studies of basic biological and biophysical processes at the molecular and cellular level, are required so that we understand what processes the cells need help with ... dispersed cells into multi-cellular spheres by rotation in suspension, and culture of spheres in regular culture dishes, spinner flasks, or in conical tubes within roller drums [98, 99] Suspension...
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Nanofibrous mat for tissue engineering, wound dressing and dermal reconstitution
... hypodermis comprises a looser connective tissue that lies beneath the dermis The skin possesses sensory receptors, hair follicles for the production and growth of hair, and sweat glands which primarily ... trypsinized and passaged at 1:3 ratios Second to fifth passages of HDFs were used in this study 3.5 HDFs seeding onto PCL/gelatin scaffolds and TG-NF constructs HDFs were detached with 1X trypsin ... scaffolds as control For a separate set of study, HDF cells were also seeded separately onto the PCL/gelatin nanofiber scaffolds as well as tissue culture plastic (TCPS) of the 24-well plates, which...
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báo cáo hóa học: " An overview of tissue engineering approaches for management of spinal cord injuries" docx
... to the host cells [126] These apparently disparate findings may be due to the issues such as the employed technique, the subpopulation of the HSCs used, and the experimental model A phase I clinical ... organized and linear axonal growth into the agarose The implant was also penetrated with Schwann cells, blood vessels and macrophages Agarose did not evoke fibrous tissue encapsulation in host tissue ... of SCI Microcapsules protect fibroblasts from the host immune response and eliminate the need for immunosuppressive therapy These constructs were injected to the spinal cord in a rat model of SCI...
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Báo cáo toán học: " Reverse Engineering Technologies for Remanufacturing of Automotive Systems Communicating via CAN Bus" pot
... steps above, it is possible to setup the desired restbus simulation for the reference system 4.4 Sensors Besides the CAN data, analog inputs of sensors and analog outputs of actuators are important ... is possible to run and test the mechatronic system outside the car as well as to adopt the results for remanufacturing the system in series production scale The same principle can also be applied ... growing new business with these systems; and the customers that are benefitting from cheaper, but as good as new, spare parts Progress is not possible without its challenges, but it is achievable...
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báo cáo khoa học:" Gene-enhanced tissue engineering for dental hard tissue regeneration: (2) dentin-pulp and periodontal regeneration" pptx
... scaling and root planing and surgical procedures such as open flap debridement for access and resective techniques, are designed to remove diseased tissue and promote an ideal environment for periodontal ... tooth-like tissues when grown in kidney capsules [41] Supplementation of autologous tooth-derived progenitor stem cells with supraphysiologic levels of recombinant growth factors appears to hold promise ... effectiveness A systematic review of published clinical trials [63] suggests that Emdogain™ affords results similar to those seen with the use of GTR Platelet-rich plasma (PRP) is a component of...
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Composite controlled release system of nitric oxide for cardiovascular tissue engineering application
... - xv - List of Abbreviations PNPE Polynitrosated Polyesters PP Polypropylene PPB Potassium Phosphate Buffer PPO Poly(propylene oxide) PS Penicillin-Streptomycin PU Polyurethane PVA Poly(vinyl ... techniques are also included Chapter presents the synthesis and properties of the novel NOreleasing gelatin-siloxane nanoparticles (GS-NO NPs) and their effects on vSMCs and ECs Chapter focus on ... Abstract Current synthetic vascular grafts for the reconstructive bypass surgeries face the problem of post-surgery restenosis Localised sustained release of nitric oxide (NO) is a promising strategy...
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Improving the mechanical and functional performance of extrusion based additive manufactured scaffolds for bone tissue engineering
... days on PCL/TCP and PCL/TCP(Si) scaffolds 72 Figure 4.12 Compressive modulus (a) and (b) compressive strength of PCL/TCP and PCL/TCP(POSS) scaffolds with various content of POSS referred to PCL/TCP ... 4.14 XPS spectra of (a) PCL/TCP and (b) PCL/TCP(POSS) scaffolds 79 Figure 4.15 Shear viscosity vs shear rate of PCL, PCL/TCP and PCL/TCP(POSS) with various content of POSS referred to PCL/TCP 79 ... width SBF - Simulated body fluid SES - Screw extrusion system SLA - Stereolithography apparatus SLS - Selective laser sintering ST - Slice thickness TCP - Tri calcium phosphate XPS - X-ray photo...
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Synthesis and characterization of collagen terpolymer fibers for applications in tissue engineering
... acetylene (PA) Poly paraphenylene (PPP) Poly paraphenylene sulfide (PPS) Poly paravinylene (PPv) Poly pyrrole (PPy) Poly thiophene (PT) Poly isothionaphthene (PITN) Poly aniline (PANI) Maximum Conductivity ... fibrous scaffold which possesses collagen proteins for cellular interactions and mechanical properties suitable for tissue engineering applications The polyelectrolyte pair we are using in this study ... and synaptophysin in hMSCs cultured on PPyrPEC fibers in the presence of electrical stimulation (ES PPyrPEC fibers), PPyrPEC fibers without electrical stimulation (PPyrPEC fibers) and on glass...
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An advanced biohybrid nano microscaffold for tendon ligament tissue engineering
... alternatives to harvested tissues, implants, and prostheses Tissue engineering has been defined as “an interdisciplinary field that applies the principles of engineering and the life sciences toward ... demand for transplant organs and tissues far exceeds the supply, and increases in the aging population only serves to widen this gap further Tissue engineering holds tremendous promise for creating ... development A bioreactor-based approach allows better understanding of tissue development and the mechanisms of disease and offers several exciting prospects, such as off-the-shelf provision of essential...
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Tissue engineering approach for annulus fibrosus regeneration
... macrophages enter the disc as a response The machophages produce cytokines such as IL-1, IFN and TNF-α, which inhibits the synthesis of matrix proteins and increases the production of MMPs (Kobayashi ... most notable and overwhelming problem with arthrodesis surgery is, persistant axial back pain That is, there is a persistent disassociation between a radiographically successful fusion and successful ... scaffolds for the NP 10 and AF Most reports use assessment methods like increased DNA, increased 11 proteoglycan synthesis and gene expression to determine the suitability of the scaffold for 12 IVD tissue...
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Differentiation and derivation of lineage committed chondroprogenitors and chondrogenic cells from human embryonic stem cells for cartilage tissue engineering and regeneration
... repair Methods: With the aim to verify the above hypothesis, a step-wise approach was designed They are as follows: Phase I: To establish hESCs as a model system to study chondrogenesis Phase ... sources: mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs) (Lee et al., 2006) Adult MSCs isolated from various adult tissues including the bone marrow, synovium, muscle, adipose tissue ... animal-derived serum replacers and mouse feeders poses the risk of hESCs taking up substantial amounts of potentially immunogenic non-human sialic acid Neu5Gc, making hESCs susceptible to immune attacks upon...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose