microenvironmental factors influence stem cell chondrogenesis
Dynamic mechanical stimulation for mesenchymal stem cell chondrogenesis in an elastomeric scaffold
Ngày tải lên :
09/09/2015, 08:12
... This process is precisely controlled by growth factors, transcriptional factors, cell- cell and cell- matrix interactions and other environmental factors (Goldring et al., 2006; Mahmoudifar & Doran, ... mesenchymal cells into precartilage condensations (Fig 5) Cellular condensation is dependent upon signals initiated by cell cell and cell matrix interactions and is associated with increased cell adhesion, ... following sections, an elaboration on cell sources, in particular that of stem cells, and the biomaterials, for cartilage tissue engineering will be addressed 2.3.1 Stem cell based Approaches for Cartilage...
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Autoimmune Diseases – Contributing Factors, Specific Cases of Autoimmune Diseases, and Stem Cell and Other Therapies pdf
Ngày tải lên :
16/03/2014, 21:20
... infiltrate showed that most cells corresponded to T cells, with T cytotoxic/suppressor cells being most abundant, although helper CD4+ T cells and NK cells Immunologic and Genetic Factors in Type Diabetes ... T cells in human and NOD mice type (DM) Autoimmune Diseases – 32 Contributing Factors, Specific Cases of Autoimmune Diseases, and Stem Cell and Other Therapies Phenotyping the infiltrating cells ... of the infiltrating cells is compatible with a cytotoxic T cell mediated beta -cell destruction, it has not yet been proven that the cells in the insulitic lesion are the cells that are actually...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
Ngày tải lên :
18/06/2014, 16:20
... administration or neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant ... bone marrow stem cells in ischemic brain Acta Neurochir Suppl 2006, 99:123-124 Li J, Sun CR, Zhang H, Tsang KS, Li JH, Zhang SD, An YH: Cotransplantation of neural stem cells and Schwann Cells results ... in the lesioned micro-environment of the CNS Materials and methods Culture of Neural stem cells Neural stem cells were harvested from the cortex of E16 Sprague-Dawley rat embryos The head was...
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Báo cáo sinh học: " Risk factors in the development of stem cell therapy" pdf
Ngày tải lên :
18/06/2014, 19:20
... accompanied by cell sorting to minimise the number of pluri/ multipotent stem cells in the cell preparation [86], or to separate tumourigenic stem cells from non-tumourigenic stem cells (by e.g cell sorting ... cultured cells, including stem cells These risk factors are not unique to stem cells and apply to all cell based medicinal products Donor history is of particular importance for stem cell lines ... Approach for the Generation of Induced Pluripotent Stem Cells Cell Stem Cell 2008, 2:525-528 81 Uccelli A, Moretta L, Pistoia V: Mesenchymal stem cells in health and disease Nature Reviews Immunology...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 2
Ngày tải lên :
11/09/2015, 16:02
... ES cells, shRNAs were used to deplete Lefty2 in E14 cells and the Lefty2 knocked down cells were observed for signs of differentiation This experiment was carried out under normal mouse ES cell ... mouse ES cell cultures, as observed with immunofluorescence microscopy Cell nuclei were stained with DAPI Magnification used was100x To determine what cell types the Lefty2 RNAi knockdown cells ... Lefty2 shRNA knockdown to differentiate the ES cells The cells were then dissociated with trypsin and replated at single cell density to allow the ES cells to expand into colonies It was interesting...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 3
Ngày tải lên :
11/09/2015, 16:02
... implicated in embryonic development and stem cell- specific expression of Lefty has been observed in mouse and human, its functional significance in embryonic stem cells has never been demonstrated ... role(s) in embryonic stem cells To elucidate the biological role(s) of Lefty2, loss of function studies using shRNAs was carried out I was able to show that Lefty2 RNAi induces ES cell differentiation ... that LEFTY2 is secreted by mouse ES cell as a negative feedback mechanism to autoregulate autocrine Nodal signals 157 How Nodal signals dictate embryonic stem cell fates is also not entirely clear...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 4
Ngày tải lên :
11/09/2015, 16:02
... ES cell differentiation assay 4.16.1 Trophoblast stem cell derivation and giant cell differentiation assay Mouse feeder conditioned medium is required as part of the culture condition for TS cells ... transfection 4.5.1 Mouse ES cells and HEK293 Transfection of plasmids into mouse ES cells and HEK293 cells were performed using Lipofectamine2000 (Invitrogen) 167 4.5.2 Human ES cell transfection For ... medium, few cells remained in the untransfected control wells indicating that selection was effective The surviving cells were trypsinized and resuspended in ES cell medium 300 or 500 cells were...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency 5
Ngày tải lên :
11/09/2015, 16:02
... human embryonic stem cells in the absence of feeder layers Stem Cells 23, 489-495 Besser, D (2004) Expression of nodal, lefty-a, and lefty-B in undifferentiated human embryonic stem cells requires ... embryonic stem cells Stem Cells 24, 1476-1486 Xu, R H., Chen, X., Li, D S., Li, R., Addicks, G C., Glennon, C., Zwaka, T P., and Thomson, J A (2002) BMP4 initiates human embryonic stem cell differentiation ... pluripotency sustaining factor in embryonic stem cells Cell 113, 643-655 Chambers, I., and Smith, A (2004) Self-renewal of teratocarcinoma and embryonic stem cells Oncogene 23, 7150-7160 Chen, C.,...
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Identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:02
... human somatic cells into pluripotent stem cells, designated as induced pluripotent stem (iPS) cells Mouse and human iPS cells could be induced by overexpressing the transcription factors quartet ... pluripotent cell lines such as ES cells, EG cells and EC cells It is now known that Nanog is regulated by OCT4, through a direct cooperative interaction with SOX2 in ES cells Nanog null ES cells lose ... microenvironment and also by intracellular regulators such as transcriptional factors Multiple factors are required to act in concert to maintain the embryonic stem cell phenotype Some factors regulate only...
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Appendix identification of factors involved in the maintenance of embryonic stem cell self renewal and pluripotency
Ngày tải lên :
11/09/2015, 16:03
... target in mouse Es cells LeftyB (human Lefty1) is regulated by Wnt pathway High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed ... ES cells LeftyA (human Lefty2) is regulated by Wnt pathway Expression detected in ES cells and F9 murine teratocarcinoma Differential expression pattern between ES cells and differentiated cells ... antagonist Criteria met • • • • High expression in ES cells and ICM Differential expression pattern between ES cells and differentiated cells Co-expressed with ES transcription factor like Nanog...
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Tế bào gốc (Stem cell)
Ngày tải lên :
18/08/2012, 20:56
... loại tế bào gốc trưởng thành giai đoạn biệt hóa thấp c/ Tế bào gốc trưởng thành (Adult stem cells/Somatic stem cells): Còn gọi tế bào gốc thân Là tế bào chưa biệt hóa tìm thấy với số lượng mô người ... tế bào gốc thai, tế bào gốc trưởng thành a/ Tế bào gốc phôi (Embryonic stem cells-ESCs) tế bào mầm phôi (Embryonic germ cells) Tế bào gốc phôi tế bào gốc vạn lấy từ phôi giai đoạn sớm (4-7 ngày ... tế bào, mô định Các tế bào gốc phôi lấy từ khối tế bào bên (inner cell mass) tế bào gốc vạn c/ Tế bào gốc đa (multipotent stem cells): Là tế bào có khả biệt hóa thành nhiều loại tế bào thể từ...
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Tài liệu Review of Chronic Graft- Versus-Host Disease in Children After Allogeneic Stem Cell Transplantation: Nursing Perspective pptx
Ngày tải lên :
12/02/2014, 19:20
... common after peripheral blood stem cell transplantation than BMT because it is generally accepted that peripheral blood stem cells con tain greater numbers of infused T-cells (Bishop, 2009; Schmitz ... graft-versus-host disease; PBSCT, peripheral blood stem cell transplantation; BMT, bone marrow transplantation; UBMT, unrelated bone marrow transplantation; T-UBMT, T -cell- depleted bone marrow transplantation; ... CINAHL, and ProQuest Nursing Allied Health were searched using the keywords “risk factors, ” “chronic GVHD,” and stem cell transplantation.” No limits were set on the searches in terms of date or...
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Tài liệu INNOVATIONS IN STEM CELL TRANSPLANTATION doc
Ngày tải lên :
18/02/2014, 04:20
... any cell type A stem cell does not differentiate directly to a specialized cell, however There are often multiple intermediate stages A stem cell will first differentiate to a progenitor cell ... investigating the role of stem cells in cell transplantation or regenerative medicine increased remarkably across all sections of the stem cell related journals The largest number of stem cell articles was ... aspects of stem cells and cell transplantation Although we are close to finding pathways for stem cell therapies in many medical conditions, scientists need to be careful how they use stem cells ethically...
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Tài liệu New Advances in Stem Cell Transplantation Edited by Taner Demirer ppt
Ngày tải lên :
20/02/2014, 08:20
... remain a stem cell, or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell Because of the unique abilities of stem cells, as ... Keratinocytes ND ND Neural stem cells
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Guidelines for Human Embryonic Stem Cell Research potx
Ngày tải lên :
06/03/2014, 15:20
... this report are common to other areas of human stem cell research, such as research with adult stem cells, fetal stem cells, or embryonic germ cells derived from fetal tissue Institutions and ... Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Introduction Stem cells are capable of self-renewal and also of differentiation into specialized cells Some stem cells are more ... types of cells It is believed that stem cells also form reservoirs of repair cells to replace cells and tissues that degenerate over the life span of the organism The dual capacity of stem cells...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
Ngày tải lên :
07/03/2014, 00:20
... disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells with a retroviral ... Ptb) ⁄ ) ES cells was reduced as compared with wild-type cells (C) Impairment of cell proliferation seen in the Ptb) ⁄ ) ES cells was rescued by ectopic expression of PTB Ptb) ⁄ ) ES cell clones ... ) ES cells exhibit reduced cell proliferation A Relative gene expression although the expression of a part of ES cell- specific markers was reduced in Ptb) ⁄ ) ES cells, both Ptb) ⁄ ) ES cell clones...
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Báo cáo " Objective and Subjective Factors Influence on Demand of Drainage by Pumping in Red River Delta " potx
Ngày tải lên :
14/03/2014, 15:20
... on planning of drainage system The design parameters of the drainage system are affected by a series of factors therefore the consultants seem to choose the negative factors for D.N Hanh / VNU ... drainage coefficient is by the subject factors than objective factors Beside the above analysis, there may be an impact which is media commucation This impact influences in decision making process ... and factors impact on drainage requirement and shows that rainfall seems not to be an objective factor Socio-economic, changing in cropping pattern or other factors might be the subjective factors...
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Commercializing the stem cell sciences pot
Ngày tải lên :
20/03/2014, 06:20
... the stem cell sciences towards long-term goals Several companies are successfully developing with this model, including Stem Cells Inc (http://www stemcellsic.com), Cellartis (http://www.cellartis.com) ... to cell culturing, eight are related to antibodies and twelve are specific stem cell factors that have been differentiated from both human and mouse stem cells Outside of these products, Stem Cells ... technologies 2.1.2 Stem cell tourism Stem cell tourism is another area that is having a significant impact on the potential market share of stem cell products A form of medical tourism, stem cell tourism...
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